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STK17A (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  STK17A
Gene Name:  serine/threonine kinase 17a
Sequence             
Original Clone ID: FLH138535.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00037970

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1245nts         Open reading frame : 1 to 1245
Reference Sequence Alignment

ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1362
Start on reference sequence 118
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK17A
Symbol Nomenclature : STK17A
SYNONYM : DRAK1
Designation : DAP kinase-related apoptosis-inducing protein kinase 1|death-associated protein kinase-related 1|serine/threonine kinase 17a (apoptosis-inducing)|serine/threonine-protein kinase 17A
Full Nomenclature : serine/threonine kinase 17a
GENEID : 9263
HGNC : 11395
MIM : 604726
Vega : OTTHUMG00000022825
Target GenBank: NM_004760

 

Reference Sequence Alignment

  

HsCD00037970      ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
NM_004760.2       ATGATCCCTTTGGAGAAGCCAGGCAGCGGCGGCTCCTCCCCAGGCGCCACCTCAGGCTCG
                  ************************************************************

HsCD00037970      GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
NM_004760.2       GGCCGGGCAGGCCGGGGTCTGAGCGGGCCGTGCCGGCCGCCGCCGCCGCCCCAGGCCCGC
                  ************************************************************

HsCD00037970      GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
NM_004760.2       GGGCTGCTGACAGAGATACGCGCCGTGGTGCGCACCGAGCCCTTCCAGGACGGCTACAGC
                  ************************************************************

HsCD00037970      CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
NM_004760.2       CTGTGCCCGGGCCGGGAGCTGGGCAGGGGGAAATTTGCAGTGGTGAGAAAATGTATAAAG
                  ************************************************************

HsCD00037970      AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
NM_004760.2       AAAGATTCTGGGAAAGAATTTGCTGCAAAGTTCATGAGAAAAAGAAGAAAAGGCCAAGAT
                  ************************************************************

HsCD00037970      TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
NM_004760.2       TGTCGGATGGAAATAATTCATGAGATTGCTGTACTTGAACTAGCACAAGACAATCCTTGG
                  ************************************************************

HsCD00037970      GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
NM_004760.2       GTCATTAATTTACATGAAGTTTATGAGACTGCATCAGAAATGATCTTAGTTCTGGAATAT
                  ************************************************************

HsCD00037970      GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
NM_004760.2       GCTGCTGGGGGTGAAATCTTTGACCAGTGTGTTGCAGACAGAGAAGAAGCCTTTAAAGAA
                  ************************************************************

HsCD00037970      AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
NM_004760.2       AAAGATGTTCAAAGACTTATGCGACAGATTTTAGAAGGTGTTCACTTTTTACACACTCGT
                  ************************************************************

HsCD00037970      GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
NM_004760.2       GATGTAGTTCATCTTGATTTGAAGCCTCAGAATATTCTGTTGACAAGTGAATCTCCATTG
                  ************************************************************

HsCD00037970      GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
NM_004760.2       GGTGACATTAAGATTGTTGATTTTGGCCTTTCAAGAATATTGAAGAACAGTGAAGAGCTC
                  ************************************************************

HsCD00037970      CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
NM_004760.2       CGAGAAATTATGGGTACCCCTGAATATGTGGCTCCTGAAATTCTTAGTTATGATCCTATA
                  ************************************************************

HsCD00037970      AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
NM_004760.2       AGCATGGCAACAGATATGTGGAGCATTGGAGTGTTAACATATGTCATGCTTACAGGAATA
                  ************************************************************

HsCD00037970      TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
NM_004760.2       TCACCTTTCTTAGGCAATGATAAACAAGAAACATTCTTAAACATCTCACAGATGAATTTA
                  ************************************************************

HsCD00037970      AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
NM_004760.2       AGTTATTCTGAGGAAGAATTTGATGTTTTGTCTGAGTCGGCTGTTGATTTCATCAGGACA
                  ************************************************************

HsCD00037970      CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
NM_004760.2       CTTTTAGTTAAGAAACCTGAAGATCGAGCCACTGCTGAAGAATGTCTAAAGCACCCCTGG
                  ************************************************************

HsCD00037970      TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
NM_004760.2       TTGACACAGAGCAGTATTCAAGAGCCTTCTTTCAGGATGGAAAAGGCACTAGAAGAAGCA
                  ************************************************************

HsCD00037970      AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
NM_004760.2       AATGCCCTCCAAGAAGGTCATTCTGTGCCTGAAATTAATTCGGATACCGACAAATCAGAA
                  ************************************************************

HsCD00037970      ACCGAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
NM_004760.2       ACCAAGGAATCCATTGTAACCGAAGAGTTAATTGTAGTTACTTCATATACTCTAGGACAA
                  ***.********************************************************

HsCD00037970      TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
NM_004760.2       TGCAGACAGTCTGAAAAAGAGAAAATGGAGCAAAAGGCCATTTCCAAACGATTTAAATTT
                  ************************************************************

HsCD00037970      GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTTG
NM_004760.2       GAGGAACCTTTGCTACAAGAAATTCCAGGAGAATTTATCTACTGA
                  ******************************************* .

Clone

Cloning Information : 138535
HIP Master Clone ID : 87199
Original Clone ID : FLH138535.01L

Organism


TAX_ID : 9606
Species Specific ID: 9263

Genome

Chromosome : 7
Map Location : 7p13
Ensembl : ENSG00000164543

Reagents

Labome : STK17A-antibody