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TK2 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TK2
Gene Name:  thymidine kinase 2, mitochondrial
Sequence             
Original Clone ID: FLH138568.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038003

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 705nts         Open reading frame : 1 to 705
Reference Sequence Alignment

ATGGGTGCGTTCTGCCAGCGTCCTAGCAGTGATAAAGAACAGGAAAAAGAGAAAAAATCA
GTGATCTGTGTCGAGGGCAATATTGCAAGTGGGAAGACGACATGCCTGGAATTCTTCTCC
AACGCGACAGACGTCGAGGTGTTAACGGAGCCTGTGTCCAAGTGGAGAAATGTCCGTGGC
CACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACAGACT
TATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACGGTTG
ATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAGTGGG
AAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAGGAAC
ATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTACCAG
AGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGAAGCA
ATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGCCCCT
GTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACAAAAT
CGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 713
Start on reference sequence 9
Mutations:
None
Discrepancy:
g88a,G30S
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TK2
Symbol Nomenclature : TK2
SYNONYM : MTDPS2; MTTK
Designation : mt-TK
Full Nomenclature : thymidine kinase 2, mitochondrial
GENEID : 7084
HGNC : 11831
MIM : 188250
Target GenBank: NM_004614

 

Reference Sequence Alignment

  

HsCD00038003        ------------------------------------------------------------
NM_004614.4         ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCGGGCGCTGCGCTGCTTTGGGCCGGGA
NM_001172645.1      ------------------------------------------------------------
NM_001172643.1      ------------------------------------------------------------
NM_001271934.1      ------------------------------------------------------------
NM_001172644.1      ------------------------------------------------------------
                                                                                

HsCD00038003        ---------------------------------ATGGGTGCGTTCTGCCAGCGTCCTAGC
NM_004614.4         AGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCGGAGGGTGCAGCGCCGGGCCTGGCCT
NM_001172645.1      -------------------------ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCG
NM_001172643.1      ---------------------------------ATGGGTGCGTTCTGCCAGCGTCCTAGC
NM_001271934.1      ------------------------------------------------------------
NM_001172644.1      -------------------------ATGCTGCTGTGGCCGCTGCGGGGCTGGGCCGCCCG
                                                                                

HsCD00038003        AGTGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_004614.4         CCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_001172645.1      GGCGCTGCGCTGCTTTGGGCCGGGAAGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCG
NM_001172643.1      AGTGATAAAGAACAGGAAAAAGAGAAAAAATCAGTGATCTGTGTCGAGGGCAATATTGCA
NM_001271934.1      ---------------------------ATGACAAGTATCTGTGTCGAGGGCAATATTGCA
NM_001172644.1      GGCGCTGCGCTGCTTTGGGCCGGGAAGTCGCGGGAGCCCGGCCTCAGGCCCCGGGCCGCG
                                               : .  ..  . * *  **..*  *.. .  **.

HsCD00038003        AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_004614.4         AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001172645.1      GAGGGTGCAGCGCCGGGCCTGGCCTCCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGT
NM_001172643.1      AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001271934.1      AGTGGGAAGACGACATGCCTGGAATTCTTCTCCAACGCGACAGACGTCGAGGTGTTAACG
NM_001172644.1      GAGGGTGCAGCGCCGGGCCTGGCCTCCCGATAAAGAACAGGAAAAAGAGAAAAAATCAGT
                    .. ** ....**.*. ******..* *  .*..*...*.. *.*.. .**..:.:*.*  

HsCD00038003        -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_004614.4         -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001172645.1      GATCTGTGTCGAGGGCAATATTGCAAGTGGGAAGACGACATGCCTGGAATTCTTCTCCAA
NM_001172643.1      -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001271934.1      -------------------------------GAGCCTGTGTCCAAGTGGAGAAATGTCCG
NM_001172644.1      GGTGTTAACGGAG---------------------CCTGTGTCCAAGTGGAGAAATGTCCG
                                                      .* . .* *.:* ..: .::   *..

HsCD00038003        TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_004614.4         TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172645.1      CGCGACAGACGTCGAGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172643.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001271934.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
NM_001172644.1      TGGCCACAATCCTCTGGGCCTGATGTACCACGATGCCTCTCGCTGGGGTCTTACGCTACA
                     *  ....*     :*********************************************

HsCD00038003        GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_004614.4         GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172645.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172643.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001271934.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
NM_001172644.1      GACTTATGTGCAGCTCACCATGCTGGACAGGCATACTCGTCCTCAGGTGTCATCTGTACG
                    ************************************************************

HsCD00038003        GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_004614.4         GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172645.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172643.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001271934.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
NM_001172644.1      GTTGATGGAGAGGTCGATTCACAGCGCAAGATACATTTTTGTAGAAAACCTGTATAGAAG
                    ************************************************************

HsCD00038003        TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_004614.4         TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172645.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172643.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001271934.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
NM_001172644.1      TGGGAAGATGCCAGAAGTGGACTATGTAGTTCTGTCGGAATGGTTTGACTGGATCTTGAG
                    ************************************************************

HsCD00038003        GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_004614.4         GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172645.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172643.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001271934.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
NM_001172644.1      GAACATGGACGTGTCTGTTGATTTGATAGTTTACCTTCGGACCAATCCTGAGACTTGTTA
                    ************************************************************

HsCD00038003        CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_004614.4         CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172645.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172643.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001271934.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
NM_001172644.1      CCAGAGGTTAAAGAAGAGATGCAGGGAAGAGGAGAAGGTCATTCCGCTGGAATACCTGGA
                    ************************************************************

HsCD00038003        AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_004614.4         AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172645.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172643.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001271934.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
NM_001172644.1      AGCAATTCACCATCTCCATGAGGAGTGGCTCATCAAAGGCAGCCTTTTCCCCATGGCAGC
                    ************************************************************

HsCD00038003        CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_004614.4         CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172645.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172643.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001271934.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
NM_001172644.1      CCCTGTTCTGGTGATTGAGGCTGACCACCACATGGAGAGGATGTTAGAACTCTTTGAACA
                    ************************************************************

HsCD00038003        AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATTG
NM_004614.4         AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172645.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172643.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001271934.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
NM_001172644.1      AAATCGGGATCGAATATTAACTCCAGAGAATCGGAAGCATTGCCCATAG
                    ***********************************************:*

Pathways

NCI : E2F transcription factor network
NCI : Validated targets of C-MYC transcriptional activation
Panther : Salvage pyrimidine deoxyribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : P04183
HPRD : 01770

Clone

Cloning Information : 138568
HIP Master Clone ID : 87232
Original Clone ID : FLH138568.01L

Organism


TAX_ID : 9606
Species Specific ID: 7083

Genome

Chromosome : 16
Map Location : 16q22-q23.1
Ensembl : ENSG00000166548

Reagents

Labome : TK2-antibody