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SUDD (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RIOK3
Gene Name:  RIO kinase 3
Sequence             
Original Clone ID: FLH138572.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 24807708
Title: RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production.
Authors:
HsCD00038007

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1560nts         Open reading frame : 1 to 1560
Reference Sequence Alignment

ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGC
AAGTGTCCATGGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGT
GAACAGCTGGCCAAAGAATTGCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCT
GTTGCTGAAGGACCATTTATTACTGGAGAAAACATTGATACTTCCAGTGACCTTATGCTG
GCTCAGATGCTACAGATGGAATATGACAGAGAATATGATGCACAGCTTAGGCGTGAAGAA
AAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCGAAAAGTGCAT
CCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAA
GATATCACCACCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAA
AATTTTGCACCTGAGTTTCAGGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAAC
CATGTTTTCAATGCTTTAAAACAACATGCCTACTCAGAAGAACGTCGAAGTGCCCGCCTA
CATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTGATCCTAAGACACGTTTACTT
ATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTATTAGTACAGGA
AAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAG
AATCGTGACAAATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAAT
CCACGTAAGATCATCCGCATGTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAG
AGAGCTGGAATTCCTTGTCCAACAGTTGTACTACTGAAGAAACACATTTTAGTTATGTCT
TTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAGAAGTAAAGCTCAATAGTGAA
GAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATATCATGAATGT
ACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG
TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTC
CGGGACTGCAGGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGT
GAACGAGAACTCTTCAATGCTGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGAT
TTTTTAGCTGAGATAGAAGCTTTGGAGAAAATGAATGAAGATCACGTTCAGAAGAATGGA
AGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCACCACTACTATATGATGAATTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1653
Start on reference sequence 94
Mutations:
None
Discrepancy:
c973a,a974t,H325I
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: EvNO00023114
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RIOK3
Symbol Nomenclature : RIOK3
SYNONYM : SUDD
Designation : homolog of the Aspergillus nidulans sudD gene product|serine/threonine-protein kinase RIO3|sudD (suppressor of bimD6, Aspergillus nidulans) homolog|sudD homolog|sudD suppressor of Aspergillus nidulans bimD6 homolog|sudD suppressor of bimD6 homolog
Full Nomenclature : RIO kinase 3
GENEID : 8780
HGNC : 11451
MIM : 603579
Vega : OTTHUMG00000131813
Target GenBank: NM_003831

 

Reference Sequence Alignment

  

HsCD00038007      ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGC
NM_003831.3       ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGC
                  ************************************************************

HsCD00038007      AAGTGTCCATGGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGT
NM_003831.3       AAGTGTCCATGGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGT
                  ************************************************************

HsCD00038007      GAACAGCTGGCCAAAGAATTGCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCT
NM_003831.3       GAACAGCTGGCCAAAGAATTGCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCT
                  ************************************************************

HsCD00038007      GTTGCTGAAGGACCATTTATTACTGGAGAAAACATTGATACTTCCAGTGACCTTATGCTG
NM_003831.3       GTTGCTGAAGGACCATTTATTACTGGAGAAAACATTGATACTTCCAGTGACCTTATGCTG
                  ************************************************************

HsCD00038007      GCTCAGATGCTACAGATGGAATATGACAGAGAATATGATGCACAGCTTAGGCGTGAAGAA
NM_003831.3       GCTCAGATGCTACAGATGGAATATGACAGAGAATATGATGCACAGCTTAGGCGTGAAGAA
                  ************************************************************

HsCD00038007      AAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCGAAAAGTGCAT
NM_003831.3       AAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCGAAAAGTGCAT
                  ************************************************************

HsCD00038007      CCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
NM_003831.3       CCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT
                  ************************************************************

HsCD00038007      CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAA
NM_003831.3       CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAA
                  ************************************************************

HsCD00038007      GATATCACCACCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAA
NM_003831.3       GATATCACCACCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAA
                  ************************************************************

HsCD00038007      AATTTTGCACCTGAGTTTCAGGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAAC
NM_003831.3       AATTTTGCACCTGAGTTTCAGGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAAC
                  ************************************************************

HsCD00038007      CATGTTTTCAATGCTTTAAAACAACATGCCTACTCAGAAGAACGTCGAAGTGCCCGCCTA
NM_003831.3       CATGTTTTCAATGCTTTAAAACAACATGCCTACTCAGAAGAACGTCGAAGTGCCCGCCTA
                  ************************************************************

HsCD00038007      CATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTGATCCTAAGACACGTTTACTT
NM_003831.3       CATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTGATCCTAAGACACGTTTACTT
                  ************************************************************

HsCD00038007      ATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTATTAGTACAGGA
NM_003831.3       ATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTATTAGTACAGGA
                  ************************************************************

HsCD00038007      AAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
NM_003831.3       AAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT
                  ************************************************************

HsCD00038007      AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAG
NM_003831.3       AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAG
                  ************************************************************

HsCD00038007      AATCGTGACAAATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAAT
NM_003831.3       AATCGTGACAAATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAAT
                  ************************************************************

HsCD00038007      CCACGTAAGATCATCCGCATGTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAG
NM_003831.3       CCACGTAAGATCATCCGCATGTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAG
                  ************************************************************

HsCD00038007      AGAGCTGGAATTCCTTGTCCAACAGTTGTACTACTGAAGAAACACATTTTAGTTATGTCT
NM_003831.3       AGAGCTGGAATTCCTTGTCCAACAGTTGTACTACTGAAGAAACACATTTTAGTTATGTCT
                  ************************************************************

HsCD00038007      TTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAGAAGTAAAGCTCAATAGTGAA
NM_003831.3       TTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAGAAGTAAAGCTCAATAGTGAA
                  ************************************************************

HsCD00038007      GAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATATCATGAATGT
NM_003831.3       GAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATATCATGAATGT
                  ************************************************************

HsCD00038007      ACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG
NM_003831.3       ACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG
                  ************************************************************

HsCD00038007      TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTC
NM_003831.3       TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTC
                  ************************************************************

HsCD00038007      CGGGACTGCAGGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGT
NM_003831.3       CGGGACTGCAGGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGT
                  ************************************************************

HsCD00038007      GAACGAGAACTCTTCAATGCTGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGAT
NM_003831.3       GAACGAGAACTCTTCAATGCTGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGAT
                  ************************************************************

HsCD00038007      TTTTTAGCTGAGATAGAAGCTTTGGAGAAAATGAATGAAGATCACGTTCAGAAGAATGGA
NM_003831.3       TTTTTAGCTGAGATAGAAGCTTTGGAGAAAATGAATGAAGATCACGTTCAGAAGAATGGA
                  ************************************************************

HsCD00038007      AGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCACCACTACTATATGATGAATTG
NM_003831.3       AGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCACCACTACTATATGATGAATAG
                  **********************************************************:*

Protein

SMART domain : RIO : RIO-like kinase
UniProt : B0YJ89
UniProt : O14730
HPRD : 04659

Clone

Cloning Information : 138572
HIP Master Clone ID : 87236
Original Clone ID : FLH138572.01L

Organism


TAX_ID : 9606
Species Specific ID: 8780

Genome

Chromosome : 18
Map Location : 18q11.2
Ensembl : ENSG00000101782

Reagents

Labome : RIOK3-antibody