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STX3A (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  STX3
Gene Name:  syntaxin 3
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH014211.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00003803

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 870nts         Open reading frame : 1 to 870
Reference Sequence Alignment

ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
ATTATTGGACTTTCCGTTGGGCTGAATTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1069
Start on reference sequence 200
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STX3
Symbol Nomenclature : STX3
SYNONYM : STX3A
Designation : syntaxin 3A|syntaxin-3
Full Nomenclature : syntaxin 3
GENEID : 6809
GI : 60653056
GenBank Accession : AY892306
HGNC : 11438
MIM : 600876
Vega : OTTHUMG00000167353
Target GenBank: BC007429

 

Reference Sequence Alignment

  

HsCD00003803        ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
XM_005274195.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
NM_001178040.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
NM_004177.4         ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
XM_005274198.1      ATGAAGGACCGTCTGGAGCAGCTGAAGGCCAAGCAGCTGACACAGGATGATGATACTGAT
                    ************************************************************

HsCD00003803        GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
XM_005274195.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
NM_001178040.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
NM_004177.4         GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
XM_005274198.1      GCGGTTGAGATTGCTATCGACAACACGGCTTTTATGGACGAGTTCTTTTCTGAGATTGAG
                    ************************************************************

HsCD00003803        GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
XM_005274195.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
NM_001178040.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
NM_004177.4         GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
XM_005274198.1      GAAACTCGGCTTAACATTGACAAGATCTCAGAACATGTAGAGGAGGCTAAGAAACTCTAC
                    ************************************************************

HsCD00003803        AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
XM_005274195.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
NM_001178040.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
NM_004177.4         AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
XM_005274198.1      AGTATCATTCTCTCTGCACCGATTCCAGAGCCAAAAACCAAGGATGACCTAGAGCAGCTC
                    ************************************************************

HsCD00003803        ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
XM_005274195.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
NM_001178040.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
NM_004177.4         ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
XM_005274198.1      ACGACTGAGATTAAGAAAAGGGCCAACAACGTCCGGAACAAACTGAAGAGCATGGAGAAG
                    ************************************************************

HsCD00003803        CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
XM_005274195.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
NM_001178040.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
NM_004177.4         CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
XM_005274198.1      CATATTGAAGAAGATGAGGTCAGGTCATCGGCAGACCTTCGGATTCGGAAATCCCAGCAC
                    ************************************************************

HsCD00003803        TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
XM_005274195.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
NM_001178040.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
NM_004177.4         TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
XM_005274198.1      TCTGTCCTTTCTCGGAAGTTTGTGGAGGTGATGACCAAATACAATGAAGCTCAAGTGGAC
                    ************************************************************

HsCD00003803        TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
XM_005274195.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
NM_001178040.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
NM_004177.4         TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
XM_005274198.1      TTCCGAGAACGCAGCAAAGGGCGAATCCAGCGGCAGCTCGAAATTACTGGCAAAAAGACA
                    ************************************************************

HsCD00003803        ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
XM_005274195.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
NM_001178040.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
NM_004177.4         ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
XM_005274198.1      ACCGATGAGGAGCTGGAGGAGATGTTGGAGAGTGGCAACCCGGCCATCTTCACTTCTGGG
                    ************************************************************

HsCD00003803        ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
XM_005274195.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
NM_001178040.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
NM_004177.4         ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
XM_005274198.1      ATCATTGACTCACAGATTTCCAAGCAAGCCCTCAGTGAGATTGAGGGACGACACAAGGAC
                    ************************************************************

HsCD00003803        ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
XM_005274195.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
NM_001178040.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
NM_004177.4         ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
XM_005274198.1      ATTGTGAGGCTGGAGAGCAGCATCAAGGAGCTTCACGACATGTTTATGGACATCGCCATG
                    ************************************************************

HsCD00003803        CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
XM_005274195.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
NM_001178040.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
NM_004177.4         CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
XM_005274198.1      CTGGTGGAGAATCAGGGTGAGATGTTAGATAACATAGAGTTGAATGTCATGCACACAGTG
                    ************************************************************

HsCD00003803        GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
XM_005274195.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
NM_001178040.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
NM_004177.4         GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
XM_005274198.1      GACCACGTGGAGAAGGCACGAGATGAAACGAAAAAAGCTGTGAAATACCAGAGTCAGGCC
                    ************************************************************

HsCD00003803        CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
XM_005274195.1      CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
NM_001178040.1      CGGAAGAAACTGATTTCACTCCAGACTGGTGTGGCCACCCTTGTCTTCAGATGA------
NM_004177.4         CGGAAGAAATTGATAATTATCATTGTGCTAGTAGTTGTGTTGCTGGGCATTTTAGCATTG
XM_005274198.1      CGGAAGAAGAAGATCATGATCATGATCTGCTGTATTATCCTTGCGATCATCTTAGCTTCC
                    ********. :*** :  .**.: .        .  .   *      **  * *      

HsCD00003803        ATTATTGGACTTTCCGTTGGGCTGAATTTG
XM_005274195.1      ATTATTGGACTTTCCGTTGGGCTGAATTAA
NM_001178040.1      ------------------------------
NM_004177.4         ATTATTGGACTTTCCGTTGGGCTGAATTAA
XM_005274198.1      ACCATTGGGAGCATATTTGCCTGA------
                                                  

Pathways

Panther : 5HT1 type receptor mediated signaling pathway
Panther : 5HT2 type receptor mediated signaling pathway
Panther : 5HT3 type receptor mediated signaling pathway
Panther : 5HT4 type receptor mediated signaling pathway
Panther : Beta1 adrenergic receptor signaling pathway
Panther : Beta2 adrenergic receptor signaling pathway
Panther : Beta3 adrenergic receptor signaling pathway
Panther : Cortocotropin releasing factor receptor signaling pathway
Panther : Dopamine receptor mediated signaling pathway
Panther : Opioid prodynorphin pathway
Panther : Opioid proenkephalin pathway
Panther : Opioid proopiomelanocortin pathway
Panther : Oxytocin receptor mediated signaling pathway
Panther : Thyrotropin-releasing hormone receptor signaling pathway

Protein

SMART domain : t_SNARE : Helical region found in SNAREs
SMART domain : SynN : Syntaxin N-terminal domain
UniProt : Q13277
UniProt : B4DME0
UniProt : Q53YE2
HPRD : 15985

Clone

Cloning Information : 14211
HIP Master Clone ID : 28410
Original Clone ID : FLH014211.01L

Organism


TAX_ID : 9606
Species Specific ID: 6809

Genome

Chromosome : 11
Map Location : 11q12.1
Ensembl : ENSG00000166900

Reagents

Labome : STX3-antibody