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MGC33182 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CSNK1A1L
Gene Name:  casein kinase 1, alpha 1-like
Sequence             
Original Clone ID: FLH178137.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038201

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1014nts         Open reading frame : 1 to 1014
Reference Sequence Alignment

ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
GAGGAAGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
TGGCAAGGACTAAAGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1429
Start on reference sequence 416
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSNK1A1L
Symbol Nomenclature : CSNK1A1L
SYNONYM : CK1
Designation : CKI-alpha-like|casein kinase I alpha S-like|casein kinase I isoform alpha-like
Full Nomenclature : casein kinase 1, alpha 1-like
GENEID : 122011
HGNC : 20289
Vega : OTTHUMG00000016748
Target GenBank: BC028723

 

Reference Sequence Alignment

  

HsCD00038201      ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
NM_145203.5       ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
                  ************************************************************

HsCD00038201      CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
NM_145203.5       CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
                  ************************************************************

HsCD00038201      GAGGAAGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
NM_145203.5       GAGGACGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
                  *****.******************************************************

HsCD00038201      AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
NM_145203.5       AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
                  ************************************************************

HsCD00038201      CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
NM_145203.5       CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
                  ************************************************************

HsCD00038201      TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
NM_145203.5       TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
                  ************************************************************

HsCD00038201      ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
NM_145203.5       ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
                  ************************************************************

HsCD00038201      AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
NM_145203.5       AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
                  ************************************************************

HsCD00038201      GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
NM_145203.5       GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
                  ************************************************************

HsCD00038201      CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
NM_145203.5       CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
                  ************************************************************

HsCD00038201      CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
NM_145203.5       CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
                  ************************************************************

HsCD00038201      TGGCAAGGACTAAAGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
NM_145203.5       TGGCAAGGACTAAGGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
                  *************.**********************************************

HsCD00038201      ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
NM_145203.5       ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
                  ************************************************************

HsCD00038201      AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
NM_145203.5       AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
                  ************************************************************

HsCD00038201      TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
NM_145203.5       TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
                  ************************************************************

HsCD00038201      TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
NM_145203.5       TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
                  ************************************************************

HsCD00038201      ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTTG
NM_145203.5       ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTAA
                  ****************************************************:.

Pathways

Panther : Hedgehog signaling pathway
Panther : Parkinson disease
Panther : Wnt signaling pathway

Clone

Cloning Information : 178137
HIP Master Clone ID : 134788
Original Clone ID : FLH178137.01L

Organism


TAX_ID : 9606
Species Specific ID: 122011

Genome

Chromosome : 13
Map Location : 13q13.3
Ensembl : ENSG00000180138

Reagents

Labome : CSNK1A1L-antibody