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Papd4 (Mus musculus) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Papd4
Gene Name:  PAP associated domain containing 4
Sequence             
Original Clone ID: 11944
Keyword: full-length cds
Species: Mus musculus
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: Linker not verified.
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
MmCD00382513

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1455nts         Open reading frame : 51 to 1505

AGATATACATACCCACGGGATCTGATAAAATTCATCATCATCATCATCACATGTTCCCAA
ACTCAATTTTGGGTCGTCCACCCTTCACTCCAACCCACCAACAGCATAATAACTTCTTTG
CTCTCTCACCAACTCTTTATTCACACCAGCAACTTATAGATGCACAATTCAACTTTCAAA
ATGTAGACTTGTCTAGAGCTGTGTCCTTACAGCCTCTGACATATGGAACTGTCAGTCCAA
TACAGACCTCAACATCTCCATTATTTCGAGGAAGGAAGAGAATAAGCGATGAAAAAGCTT
TTCCTCTTGATGGTAAACGGCAGCGTTTCCATTCACCCCACCAAGAGCCAACTATAATCA
ACCAGCTAGTGCCTTTATCAGGTGACAGAAGATATTCCATGCCACCGTTGTTTCACACGC
ACTATATACCAGATATAGTCAGATGTGTTCCACCTCTTCGAGAAATTCCACTGTTAGAAC
CTCGAGAAATCACACTGCCTGAGGCCAAAGATAAGTTGAGTCAGCAGATACTAGAGTTGT
TTGAAACTTGTCAGCAGCAAGCAAGTGACTTGAAGAAGAAAGAACTCTGTAGAGCACAGC
TGCAGAGAGAGATCCAGCTGCTCTTCCCACAAAGCAGACTTTTTCTCGTTGGATCATCTT
TGAATGGATTTGGTGCCCGAAGCAGTGATGGCGATTTATGCTTAGTTGTTAAAGAAGAAC
CATGTTTTTTCCAGGTAAATCAGAAGACTGAAGCACGACATATACTCACCTTAGTCCATA
AGCACTTCTGTACTAGACTGTCTGGCTATATTGAGAGACCTCAGCTGATTCGAGCAAAAG
TGCCAATTGTGAAGTTCAGGGATAAAGTCAGTTGTGTGGAATTCGACTTAAATGTAAACA
ATACCGTTGGAATTAGAAATACATTCCTTCTCAGAACCTATGCATACCTTGAAAATCGAG
TTCGTCCATTAGTGCTGGTGATTAAGAAGTGGGCAAGTCACCATGACATCAATGATGCCA
GTCGTGGCACATTAAGCAGCTATAGTCTTGTATTGATGGTTTTGCACTATTTACAAACCC
TACCTGAACCCATCCTTCCATCCCTCCAAAAAATTTACCCAGAATCTTTTAGTACTTCTG
TCCAGCTGCACCTTGTTCATCATGCCCCATGTAATGTCCCTCCTTACCTCTCAAAGAATG
AATCAAGTCTTGGGGACCTCTTACTGGGCTTTCTGAAATATTATGCTACAGAATTTGACT
GGAACACTCAGATGATTTCAGTTCGTGAAGCCAAAGCCATCCCAAGGCCTGATGATATGG
AATGGAGAAATAAATACATCTGTGTTGAAGAACCTTTTGATGGAACTAATACCGCCAGAG
CTGTGCATGAGAAGCAGAAGTTTGATATGATCAAGGATCAATTTTTAAAGTCATGGCAGA
GATTGAAAAACAAGAGAGATCTGAACAGTGTGCTGCCTTTGAGAGCTGCTACCCTGAAAA
GATAACGGGACTTAATTAACTCTAGATAACTGAGAAGGAGATATACATACCCGGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 354879
Recommended expression in: BL21Gold(DE3)pLysS
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: JCSG
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 


Cloning Information: 278542
GI: 19527122
GenBank Accession: NM_133905
HIP Master Clone ID: 208028
Labome: Papd4-antibody
Original Clone ID: 11944
Structural Annotation Wiki: TOPSAN
TargetTrack - Experimental data: 354879
UniProt: Q91YI6
Species Specific ID: 100715
Target GenBank: None