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CDC37 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CDC37
Gene Name:  cell division cycle 37
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH014458.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00003828

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1137nts         Open reading frame : 1 to 1137
Reference Sequence Alignment

ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1186
Start on reference sequence 50
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CDC37
Symbol Nomenclature : CDC37
SYNONYM : P50CDC37
Designation : CDC37 (cell division cycle 37, S. cerevisiae, homolog)|CDC37 cell division cycle 37 homolog|cell division cycle 37 homolog|hsp90 chaperone protein kinase-targeting subunit|hsp90 co-chaperone Cdc37
Full Nomenclature : cell division cycle 37
GENEID : 11140
GI : 60656136
GenBank Accession : AY889841
HGNC : 1735
MIM : 605065
Vega : OTTHUMG00000180578
Target GenBank: BC008793

 

Reference Sequence Alignment

  

HsCD00003828      ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
NM_007065.3       ATGGTGGACTACAGCGTGTGGGACCACATTGAGGTGTCTGATGATGAAGACGAGACGCAC
                  ************************************************************

HsCD00003828      CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
NM_007065.3       CCCAACATCGACACGGCCAGTCTCTTCCGCTGGCGGCATCAGGCCCGGGTGGAACGCATG
                  ************************************************************

HsCD00003828      GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
NM_007065.3       GAGCAGTTCCAGAAGGAGAAGGAGGAACTGGACAGGGGCTGCCGCGAGTGCAAGCGCAAG
                  ************************************************************

HsCD00003828      GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
NM_007065.3       GTGGCCGAGTGCCAGAGGAAACTGAAGGAGCTGGAGGTGGCCGAGGGCGGCAAGGCAGAG
                  ************************************************************

HsCD00003828      CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
NM_007065.3       CTGGAGCGCCTGCAGGCCGAGGCACAGCAGCTGCGCAAGGAGGAGCGGAGCTGGGAGCAG
                  ************************************************************

HsCD00003828      AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
NM_007065.3       AAGCTGGAGGAGATGCGCAAGAAGGAGAAGAGCATGCCCTGGAACGTGGACACGCTCAGC
                  ************************************************************

HsCD00003828      AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
NM_007065.3       AAAGACGGCTTCAGCAAGAGCATGGTAAATACCAAGCCCGAGAAGACGGAGGAGGACTCA
                  ************************************************************

HsCD00003828      GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
NM_007065.3       GAGGAGGTGAGGGAGCAGAAACACAAGACCTTCGTGGAAAAATACGAGAAACAGATCAAG
                  ************************************************************

HsCD00003828      CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
NM_007065.3       CACTTTGGCATGCTTCGCCGCTGGGATGACAGCCAAAAGTACCTGTCAGACAACGTCCAC
                  ************************************************************

HsCD00003828      CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
NM_007065.3       CTGGTGTGCGAGGAGACAGCCAATTACCTGGTCATTTGGTGCATTGACCTAGAGGTGGAG
                  ************************************************************

HsCD00003828      GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
NM_007065.3       GAGAAATGTGCACTCATGGAGCAGGTGGCCCACCAGACAATCGTCATGCAATTTATCCTG
                  ************************************************************

HsCD00003828      GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
NM_007065.3       GAGCTGGCCAAGAGCCTAAAGGTGGACCCCCGGGCCTGCTTCCGGCAGTTCTTCACTAAG
                  ************************************************************

HsCD00003828      ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
NM_007065.3       ATTAAGACAGCCGATCGCCAGTACATGGAGGGCTTCAACGACGAGCTGGAAGCCTTCAAG
                  ************************************************************

HsCD00003828      GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
NM_007065.3       GAGCGTGTGCGGGGCCGTGCCAAGCTGCGCATCGAGAAGGCCATGAAGGAGTACGAGGAG
                  ************************************************************

HsCD00003828      GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
NM_007065.3       GAGGAGCGCAAGAAGCGGCTCGGCCCCGGCGGCCTGGACCCCGTCGAGGTCTACGAGTCC
                  ************************************************************

HsCD00003828      CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
NM_007065.3       CTCCCTGAGGAACTCCAGAAGTGCTTCGATGTGAAGGACGTGCAGATGCTGCAGGACGCC
                  ************************************************************

HsCD00003828      ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
NM_007065.3       ATCAGCAAGATGGACCCCACCGACGCAAAGTACCACATGCAGCGCTGCATTGACTCTGGC
                  ************************************************************

HsCD00003828      CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
NM_007065.3       CTCTGGGTCCCCAACTCTAAGGCCAGCGAGGCCAAGGAGGGAGAGGAGGCAGGTCCTGGG
                  ************************************************************

HsCD00003828      GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTAG
NM_007065.3       GACCCATTACTGGAAGCTGTTCCCAAGACGGGCGATGAGAAGGATGTCAGTGTGTGA
                  *******************************************************..

Pathways

NCI : Integrin-linked kinase signaling
NCI : LKB1 signaling events
Reactome : Disease
Reactome : Signal Transduction
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by constitutively active EGFR
WikiPathway : TNF alpha Signaling Pathway

Clone

Cloning Information : 14458
HIP Master Clone ID : 28657
Original Clone ID : FLH014458.01X

Organism


TAX_ID : 9606
Species Specific ID: 11140

Genome

Chromosome : 19
Map Location : 19p13.2
Ensembl : ENSG00000105401

Reagents

Labome : CDC37-antibody