DNASU Plasmid Repository • 480.965.5697 | Email

DVU0982 (D. vulgaris str. Hildenborough ) in pSGX3 (His-tagged bacterial expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  DVU0982
Gene Name:  None
Sequence             
Original Clone ID: 9570b1BCt5p1
Keyword: full-length cds
Species: Desulfovibrio vulgaris str. Hildenborough
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified. Actual 5' flanking sequence provided below.
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
DvCD00383390

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 681nts         Open reading frame : 44 to 724

TAATTTTGTTTACTTTAAGAAGGAGATATACAATATGTCCCTTATCGACTTCCACGTCCA
CACCACGTTCAGCGACAGCACCATGACCCCCGCCGTGGCCGTGCGCCACGCCAAGATGGC
CGGGTATCGCGCCGTGGCACTCACCGACCACGCCGACCATGCCAACATGGACCTCATCCT
GCGCCAGACCCTGCCGCTGGCCCGCAAGTACTCACTCTACGCGGGCATCGAGGTATTCGC
GGGTGTGGAACTCACACACGTGCCCCCTGCCCTCATCCCCGAAGCCGTGCGCGAGGCACG
CGCCATGGGCGCGCAGCTTGTGCTGGTGCACGGCGAGACCATCAGCGATGCCGTCGAAAC
CGGCACCAACCTTGCCGCCATCGAAGGTGGTGTCGACATCCTGGCCCACCCCGGACTCGT
CAGTGACGAGGATGCCGCCTATGCCGCCGAACGCGGCGTGCGCTTTGAAATCACGACCCG
TCCCGGACACGCCCTCACCAACGGGCATGTCGCCATGGCGGCGCGAAGGCACGGCGTCAG
GTGCGTCATCAACAACGACGCCCACCACTCCCGTGATTTCGTGAACCCCGACCACCGCCT
TGCCGTGGCACTGGGGGCGGGGCTGACCACCGCAGAGGTGACCGCCGCAGAAGCCGAAAG
CCGCACATTGCTGGGCAGGCTGCTGCGCGAAGTCCGCGAAGGGCATCATCACCATCACCA
CTGATCCGAATTCGAGCTCCGTCGACAAGCTTGC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-9570b
Recommended expression in: BL21(DE3)-Codon+RIL
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Reagents

Gene

Target GenBank: AE017285.1

 

Protein

SMART domain : POLIIIAc : DNA polymerase alpha chain like domain
Structural Annotation Wiki : TOPSAN
UniProt : Q72DE7

Clone

Cloning Information : 486937
HIP Master Clone ID : 402385
Original Clone ID : 9570b1BCt5p1

Experimental

TargetTrack - Experimental data : NYSGXRC-9570b

Reagents

Labome : DVU0982-cDNA