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SGK2 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SGK2
Gene Name:  serum/glucocorticoid regulated kinase 2
Sequence             
Original Clone ID: FLH205267.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038405

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1101nts         Open reading frame : 1 to 1101
Reference Sequence Alignment

ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
TATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGAGCCACATCATG
GCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGCTAC
TCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAGCTC
TTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCTGCT
GAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTGAAA
CCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTCTGC
AAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTACTTG
GCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTGGGG
GCATTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCCCAG
ATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCCGCC
TGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAAGCA
GACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTGTAC
CACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAGCAT
TTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGACACT
GTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAGGAT
GATGACATCTTGGATTGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1191
Start on reference sequence 88
Mutations:
None
Discrepancy:
del@227,3;g667t,V223F;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SGK2
Symbol Nomenclature : SGK2
SYNONYM : H-SGK2; dJ138B7.2
Designation : serine/threonine-protein kinase Sgk2
Full Nomenclature : serum/glucocorticoid regulated kinase 2
GENEID : 10110
Locus Tag : RP1-138B7.2
HGNC : 13900
MIM : 607589
Vega : OTTHUMG00000033054
Target GenBank: NM_016276

 

Reference Sequence Alignment

  

HsCD00038405        ------------------------------------------------------------
NM_170693.2         ------------------------------------------------------------
NM_001199264.1      ------------------------------------------------------------
NM_016276.3         ATGCAGGGGTTGCTTACCTCGGGTAGGAAACCCTCAGGCGGTGGCAGGTGCACAGGTAGG
                                                                                

HsCD00038405        ------------------------------------------------------------
NM_170693.2         ------------------------------------------------------------
NM_001199264.1      ------------------------------------------------------------
NM_016276.3         GGAGGATGGAGAGGGCAGTGGTGCCTGAAGCCCTGGATGGGCGGAGCTGACCCCCCAACA
                                                                                

HsCD00038405        ------------------------------------------------------------
NM_170693.2         ------------------------------------------------------------
NM_001199264.1      ------------------------------------------------------------
NM_016276.3         CCAACTCTCTCATGCCTGCTCCTCCCTGTCCCCCCAGAGCTGCCTGATCATTGCTACAGA
                                                                                

HsCD00038405        ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
NM_170693.2         ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
NM_001199264.1      ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
NM_016276.3         ATGAACTCTAGCCCAGCTGGGACCCCAAGTCCACAGCCCTCCAGGGCCAATGGGAACATC
                    ************************************************************

HsCD00038405        AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
NM_170693.2         AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
NM_001199264.1      AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
NM_016276.3         AACCTGGGGCCTTCAGCCAACCCAAATGCCCAGCCCACGGACTTCGACTTCCTCAAAGTC
                    ************************************************************

HsCD00038405        ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
NM_170693.2         ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
NM_001199264.1      ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
NM_016276.3         ATCGGCAAAGGGAACTACGGGAAGGTCCTACTGGCCAAGCGCAAGTCTGATGGGGCGTTC
                    ************************************************************

HsCD00038405        TATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGAG---CCACATC
NM_170693.2         TATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGCAGAGCCACATC
NM_001199264.1      TATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGCAGAGCCACATC
NM_016276.3         TATGCAGTGAAGGTACTACAGAAAAAGTCCATCTTAAAGAAGAAAGAGCAGAGCCACATC
                    ************************************************..   *******

HsCD00038405        ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC
NM_170693.2         ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC
NM_001199264.1      ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC
NM_016276.3         ATGGCAGAGCGCAGTGTGCTTCTGAAGAACGTGCGGCACCCCTTCCTCGTGGGCCTGCGC
                    ************************************************************

HsCD00038405        TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG
NM_170693.2         TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG
NM_001199264.1      TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG
NM_016276.3         TACTCCTTCCAGACACCTGAGAAGCTCTACTTCGTGCTCGACTATGTCAACGGGGGAGAG
                    ************************************************************

HsCD00038405        CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
NM_170693.2         CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
NM_001199264.1      CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
NM_016276.3         CTCTTCTTCCACCTGCAGCGGGAGCGCCGGTTCCTGGAGCCCCGGGCCAGGTTCTACGCT
                    ************************************************************

HsCD00038405        GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG
NM_170693.2         GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG
NM_001199264.1      GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG
NM_016276.3         GCTGAGGTGGCCAGCGCCATTGGCTACCTGCACTCCCTCAACATCATTTACAGGGATCTG
                    ************************************************************

HsCD00038405        AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC
NM_170693.2         AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC
NM_001199264.1      AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC
NM_016276.3         AAACCAGAGAACATTCTCTTGGACTGCCAGGGACACGTGGTGCTGACGGATTTTGGCCTC
                    ************************************************************

HsCD00038405        TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC
NM_170693.2         TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC
NM_001199264.1      TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC
NM_016276.3         TGCAAGGAAGGTGTAGAGCCTGAAGACACCACATCCACATTCTGTGGTACCCCTGAGTAC
                    ************************************************************

HsCD00038405        TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG
NM_170693.2         TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG
NM_001199264.1      TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG
NM_016276.3         TTGGCACCTGAAGTGCTTCGGAAAGAGCCTTATGATCGAGCAGTGGACTGGTGGTGCTTG
                    ************************************************************

HsCD00038405        GGGGCATTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC
NM_170693.2         GGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC
NM_001199264.1      GGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC
NM_016276.3         GGGGCAGTCCTCTACGAGATGCTCCATGGCCTGCCGCCCTTCTACAGCCAAGATGTATCC
                    ****** *****************************************************

HsCD00038405        CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC
NM_170693.2         CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC
NM_001199264.1      CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC
NM_016276.3         CAGATGTATGAGAACATTCTGCACCAGCCGCTACAGATCCCCGGAGGCCGGACAGTGGCC
                    ************************************************************

HsCD00038405        GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
NM_170693.2         GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
NM_001199264.1      GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
NM_016276.3         GCCTGTGACCTCCTGCAAAGCCTTCTCCACAAGGACCAGAGGCAGCGGCTGGGCTCCAAA
                    ************************************************************

HsCD00038405        GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG
NM_170693.2         GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG
NM_001199264.1      GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG
NM_016276.3         GCAGACTTTCTTGAGATTAAGAACCATGTATTCTTCAGCCCCATAAACTGGGATGACCTG
                    ************************************************************

HsCD00038405        TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG
NM_170693.2         TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG
NM_001199264.1      TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG
NM_016276.3         TACCACAAGAGGCTAACTCCACCCTTCAACCCAAATGTGACAGGACCTGCTGACTTGAAG
                    ************************************************************

HsCD00038405        CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC
NM_170693.2         CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC
NM_001199264.1      CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC
NM_016276.3         CATTTTGACCCAGAGTTCACCCAGGAAGCTGTGTCCAAGTCCATTGGCTGTACCCCTGAC
                    ************************************************************

HsCD00038405        ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG
NM_170693.2         ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG
NM_001199264.1      ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG
NM_016276.3         ACTGTGGCCAGCAGCTCTGGGGCCTCAAGTGCATTCCTGGGATTTTCTTATGCGCCAGAG
                    ************************************************************

HsCD00038405        GATGATGACATCTTGGATTGCTTG
NM_170693.2         GATGATGACATCTTGGATTGCTAG
NM_001199264.1      GATGATGACATCTTGGATTGCTAG
NM_016276.3         GATGATGACATCTTGGATTGCTAG
                    **********************:*

Pathways

Reactome : Ion channel transport
Reactome : Stimuli-sensing channels
Reactome : Transmembrane transport of small molecules
WikiPathway : Insulin Signaling

Clone

Cloning Information : 205267
HIP Master Clone ID : 11578
Original Clone ID : FLH205267.01L

Organism


TAX_ID : 9606
Species Specific ID: 10110

Genome

Chromosome : 20
Map Location : 20q13.2
Ensembl : ENSG00000101049

Reagents

Labome : SGK2-antibody