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STK3 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  STK3
Gene Name:  serine/threonine kinase 3
Sequence             
Original Clone ID: FLH205285.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038418

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1476nts         Open reading frame : 1 to 1476
Reference Sequence Alignment

ATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAAAAGCTGAGTGAAGACAGTTTGACT
AAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAGCTTGGAGAAGGGTCTTATGGAAGT
GTATTTAAAGCAATACACAAGGAATCCGGTCAAGTTGTCGCAATTAAACAAGTACCTGTT
GAATCAGATCTTCAGGAAATAATCAAAGAAATTTCCATAATGCAGCAATGTGACAGCCCA
TATGTTGTAAAGTACTATGGCAGTTATTTTAAGAATACAGACCTCTGGATTGTTATGGAG
TACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGATTACGAAACAAGACATTAATAGAA
GATGAAATTGCAACCATTCTTAAATCTACATTGAAAGGACTAGAATATTTGCACTTTATG
AGAAAAATACACAGAGATATAAAAGCTGGAAATATTCTCCTCAATACAGAAGGACATGCA
AAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACAGATACAATGGCAAAACGCAATACT
GTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTGATTCAAGAAATAGGCTATAACTGT
GTGGCCGACATCTGGTCCCTTGGCATTACTTCTATAGAAATGGCTGAAGGAAAACCTCCT
TATGCTGATATACATCCAATGAGGGCTATTTTTATGATTCCCACAAATCCACCACCAACA
TTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACCGATTTTGTTAAAAAGTGTTTGGTG
AAGAATCCTGAGCAGAGAGCTACTGCAACACAACTTTTACAGCATCCTTTTATCAAGAAT
GCCAAACCTGTATCAATATTAAGAGACCTGATCACAGAAGCTATGGAGATCAAAGCTAAA
AGACATGAGGAACAGCAACGAGAATTGGAAGAGGAAGAAGAAAATTCGGATGAAGATGAG
CTGGATTCCCACACCATGGTGAAGACTAGTGTGGAGAGTGTGGGCACCATGCGGGCCACA
AGCACGATGAGTGAAGGGGCCCAGACCATGATTGAACATAATAGCACGATGTTGGAATCC
GACTTGGGGACCATGGTGATAAACAGTGAGGATGAGGAAGAAGAAGATGGAACTATGAAA
AGAAATGCAACCTCACCACAAGTACAAAGACCATCTTTCATGGACTACTTTGATAAGCAA
GACTTCAAGAATAAGAGTCACGAAAACTGTAATCAGAACATGCATGAACCCTTCCCTATG
TCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCTCAAGATGGAGACTTTGACTTTTTG
AAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTAAAAGCACTGGACCCCATGATGGAA
CGGGAGATAGAAGAACTTCGTCAGAGATACACTGCGAAAAGACAGCCCATTCTGGATGCG
ATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1614
Start on reference sequence 139
Mutations:
None
Discrepancy:
c909g,D303E; g995a,a996g,G332E;
 g997a,a998g,g999t,E333S; t1000g,g1001t,t1002g,C334V
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK3
Symbol Nomenclature : STK3
SYNONYM : KRS1; MST2
Designation : MST-2|STE20-like kinase MST2|mammalian STE20-like protein kinase 2|serine/threonine kinase 3 (STE20 homolog, yeast)|serine/threonine kinase 3 (Ste20, yeast homolog)|serine/threonine-protein kinase 3|serine/threonine-protein kinase Krs-1
Full Nomenclature : serine/threonine kinase 3
GENEID : 6788
HGNC : 11406
MIM : 605030
Vega : OTTHUMG00000164651
Target GenBank: NM_006281

 

Reference Sequence Alignment

  

HsCD00038418        ------------------------------------------------------------
NM_006281.3         ------------------------------------------------------------
NM_001256312.1      ATGTTACAACTCATGGACAGTGGCATTACCATCTGCCTTAGGAACGGAGCAGCCTCTGTC
                                                                                

HsCD00038418        ------------------------ATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAA
NM_006281.3         ------------------------ATGGAGCAGCCGCCGGCGCCTAAGAGTAAACTAAAA
NM_001256312.1      TTCAAGAAAAAAGAATGGAGCACACAAGGAGAAGAAAACAAGCAAGACAGTAAACTAAAA
                                            .:.*.. *. .... ..**.:.* ************

HsCD00038418        AAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAG
NM_006281.3         AAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAG
NM_001256312.1      AAGCTGAGTGAAGACAGTTTGACTAAGCAGCCTGAAGAAGTTTTTGATGTATTAGAGAAG
                    ************************************************************

HsCD00038418        CTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTT
NM_006281.3         CTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTT
NM_001256312.1      CTTGGAGAAGGGTCTTATGGAAGTGTATTTAAAGCAATACACAAGGAATCCGGTCAAGTT
                    ************************************************************

HsCD00038418        GTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCC
NM_006281.3         GTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCC
NM_001256312.1      GTCGCAATTAAACAAGTACCTGTTGAATCAGATCTTCAGGAAATAATCAAAGAAATTTCC
                    ************************************************************

HsCD00038418        ATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAAT
NM_006281.3         ATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAAT
NM_001256312.1      ATAATGCAGCAATGTGACAGCCCATATGTTGTAAAGTACTATGGCAGTTATTTTAAGAAT
                    ************************************************************

HsCD00038418        ACAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGA
NM_006281.3         ACAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGA
NM_001256312.1      ACAGACCTCTGGATTGTTATGGAGTACTGTGGCGCTGGCTCTGTCTCAGACATAATTAGA
                    ************************************************************

HsCD00038418        TTACGAAACAAGACATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAA
NM_006281.3         TTACGAAACAAGACATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAA
NM_001256312.1      TTACGAAACAAGACATTAATAGAAGATGAAATTGCAACCATTCTTAAATCTACATTGAAA
                    ************************************************************

HsCD00038418        GGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATT
NM_006281.3         GGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATT
NM_001256312.1      GGACTAGAATATTTGCACTTTATGAGAAAAATACACAGAGATATAAAAGCTGGAAATATT
                    ************************************************************

HsCD00038418        CTCCTCAATACAGAAGGACATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACA
NM_006281.3         CTCCTCAATACAGAAGGACATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACA
NM_001256312.1      CTCCTCAATACAGAAGGACATGCAAAATTGGCAGATTTTGGAGTGGCTGGTCAGTTAACA
                    ************************************************************

HsCD00038418        GATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTG
NM_006281.3         GATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTG
NM_001256312.1      GATACAATGGCAAAACGCAATACTGTAATAGGAACTCCATTTTGGATGGCTCCTGAGGTG
                    ************************************************************

HsCD00038418        ATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATA
NM_006281.3         ATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATA
NM_001256312.1      ATTCAAGAAATAGGCTATAACTGTGTGGCCGACATCTGGTCCCTTGGCATTACTTCTATA
                    ************************************************************

HsCD00038418        GAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATG
NM_006281.3         GAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATG
NM_001256312.1      GAAATGGCTGAAGGAAAACCTCCTTATGCTGATATACATCCAATGAGGGCTATTTTTATG
                    ************************************************************

HsCD00038418        ATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACC
NM_006281.3         ATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACC
NM_001256312.1      ATTCCCACAAATCCACCACCAACATTCAGAAAGCCAGAACTTTGGTCCGATGATTTCACC
                    ************************************************************

HsCD00038418        GATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTT
NM_006281.3         GATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTT
NM_001256312.1      GATTTTGTTAAAAAGTGTTTGGTGAAGAATCCTGAGCAGAGAGCTACTGCAACACAACTT
                    ************************************************************

HsCD00038418        TTACAGCATCCTTTTATCAAGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACA
NM_006281.3         TTACAGCATCCTTTTATCAAGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACA
NM_001256312.1      TTACAGCATCCTTTTATCAAGAATGCCAAACCTGTATCAATATTAAGAGACCTGATCACA
                    ************************************************************

HsCD00038418        GAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAA
NM_006281.3         GAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAA
NM_001256312.1      GAAGCTATGGAGATCAAAGCTAAAAGACATGAGGAACAGCAACGAGAATTGGAAGAGGAA
                    ************************************************************

HsCD00038418        GAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAG
NM_006281.3         GAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAG
NM_001256312.1      GAAGAAAATTCGGATGAAGATGAGCTGGATTCCCACACCATGGTGAAGACTAGTGTGGAG
                    ************************************************************

HsCD00038418        AGTGTGGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAA
NM_006281.3         AGTGTGGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAA
NM_001256312.1      AGTGTGGGCACCATGCGGGCCACAAGCACGATGAGTGAAGGGGCCCAGACCATGATTGAA
                    ************************************************************

HsCD00038418        CATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAG
NM_006281.3         CATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAG
NM_001256312.1      CATAATAGCACGATGTTGGAATCCGACTTGGGGACCATGGTGATAAACAGTGAGGATGAG
                    ************************************************************

HsCD00038418        GAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCT
NM_006281.3         GAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCT
NM_001256312.1      GAAGAAGAAGATGGAACTATGAAAAGAAATGCAACCTCACCACAAGTACAAAGACCATCT
                    ************************************************************

HsCD00038418        TTCATGGACTACTTTGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAG
NM_006281.3         TTCATGGACTACTTTGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAG
NM_001256312.1      TTCATGGACTACTTTGATAAGCAAGACTTCAAGAATAAGAGTCACGAAAACTGTAATCAG
                    ************************************************************

HsCD00038418        AACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCT
NM_006281.3         AACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCT
NM_001256312.1      AACATGCATGAACCCTTCCCTATGTCCAAAAACGTTTTTCCTGATAACTGGAAAGTTCCT
                    ************************************************************

HsCD00038418        CAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTA
NM_006281.3         CAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTA
NM_001256312.1      CAAGATGGAGACTTTGACTTTTTGAAAAATCTAAGTTTAGAAGAACTACAGATGCGGTTA
                    ************************************************************

HsCD00038418        AAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCG
NM_006281.3         AAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCG
NM_001256312.1      AAAGCACTGGACCCCATGATGGAACGGGAGATAGAAGAACTTCGTCAGAGATACACTGCG
                    ************************************************************

HsCD00038418        AAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTTG
NM_006281.3         AAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTGA
NM_001256312.1      AAAAGACAGCCCATTCTGGATGCGATGGATGCAAAGAAAAGAAGGCAGCAAAACTTTTGA
                    ********************************************************** .

Pathways

Reactome : Signal Transduction
Reactome : Signaling by Hippo
WikiPathway : MAPK signaling pathway

Clone

Cloning Information : 205285
HIP Master Clone ID : 34478
Original Clone ID : FLH205285.01L

Organism


TAX_ID : 9606
Species Specific ID: 6788

Genome

Chromosome : 8
Map Location : 8q22.2
Ensembl : ENSG00000104375

Reagents

Labome : STK3-antibody