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AK1 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  AK1
Gene Name:  adenylate kinase 1
Sequence             
Original Clone ID: FLH205286.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038419

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 585nts         Open reading frame : 1 to 585
Reference Sequence Alignment

ATGGAAGAGAAGCTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGG
AAGGGCACCCAGTGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGG
GACCTCCTGCGGTCCGAGGTCAGCTCAGGCTCGGCCACGGGCAAGAAGCTGTCGGAAATC
ATGGAGAAGGGGCAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATG
GTTGCCAAAGTCAATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAG
CAAGGAGAAGAGTTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCA
GGCCCTGAGACCATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGAC
GACAATGAGGAGACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTC
ATCGCCTTCTATGAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGAC
AGTGTCTTCTCCCAGGTCTGCACCCACCTGGACGCCCTAAAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 659
Start on reference sequence 75
Mutations:
None
Discrepancy:
g158c,R53T; g243t
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : AK1
Symbol Nomenclature : AK1
SYNONYM : HTL-S-58j
Designation : AK 1|ATP-AMP transphosphorylase 1|ATP:AMP phosphotransferase|adenylate kinase isoenzyme 1|adenylate monophosphate kinase|myokinase|testis secretory sperm binding protein Li 58j
Full Nomenclature : adenylate kinase 1
GENEID : 203
Locus Tag : RP11-203J24.1
HGNC : 361
MIM : 103000
Vega : OTTHUMG00000020722
Target GenBank: NM_000476

 

Reference Sequence Alignment

  

HsCD00038419        ------------------------------------------------ATGGAAGAGAAG
XM_005251788.1      ------------------------------------------------------------
NM_000476.2         ------------------------------------------------ATGGAAGAGAAG
XM_005251786.1      ATGGGCTGCTGCTCCTCGAGTGACCCCCGCAGAGAAGACGATCTGAGAGCCAGAGAGAAG
                                                                                

HsCD00038419        CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
XM_005251788.1      ---------ATGAATGGGTGGGCGTTTGCAGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
NM_000476.2         CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
XM_005251786.1      CTGAAGAAAACCAAGATCATCTTTGTGGTGGGTGGGCCTGGCTCAGGGAAGGGCACCCAG
                             *  ** .  :      * * .******************************

HsCD00038419        TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
XM_005251788.1      TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
NM_000476.2         TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
XM_005251786.1      TGTGAGAAGATCGTGCAGAAGTATGGCTACACCCACCTCTCCACCGGGGACCTCCTGCGG
                    ************************************************************

HsCD00038419        TCCGAGGTCAGCTCAGGCTCGGCCACGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
XM_005251788.1      TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
NM_000476.2         TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
XM_005251786.1      TCCGAGGTCAGCTCAGGCTCGGCCAGGGGCAAGAAGCTGTCGGAAATCATGGAGAAGGGG
                    ************************* **********************************

HsCD00038419        CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTTGCCAAAGTC
XM_005251788.1      CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
NM_000476.2         CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
XM_005251786.1      CAGCTGGTTCCACTGGAGACAGTGTTGGACATGCTCCGGGATGCCATGGTGGCCAAAGTC
                    ************************************************** *********

HsCD00038419        AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
XM_005251788.1      AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
NM_000476.2         AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
XM_005251786.1      AATACTTCCAAAGGCTTCCTGATTGATGGCTACCCGCGGGAGGTGCAGCAAGGAGAAGAG
                    ************************************************************

HsCD00038419        TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
XM_005251788.1      TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
NM_000476.2         TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
XM_005251786.1      TTTGAGCGACGGATTGGACAGCCCACACTGCTGCTGTATGTGGACGCAGGCCCTGAGACC
                    ************************************************************

HsCD00038419        ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
XM_005251788.1      ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
NM_000476.2         ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
XM_005251786.1      ATGACCCAGCGGCTCTTGAAACGTGGAGAGACCAGCGGGCGTGTGGACGACAATGAGGAG
                    ************************************************************

HsCD00038419        ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTCATCGCCTTCTAT
XM_005251788.1      ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCTGTCATCGCCTTCTAT
NM_000476.2         ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCCGTCATCGCCTTCTAT
XM_005251786.1      ACCATCAAAAAGCGGCTGGAGACCTATTACAAGGCCACAGAACCTGTCATCGCCTTCTAT
                    ******************************************** ***************

HsCD00038419        GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
XM_005251788.1      GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
NM_000476.2         GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
XM_005251786.1      GAGAAACGTGGCATTGTGCGCAAGGTCAACGCTGAGGGCTCCGTGGACAGTGTCTTCTCC
                    ************************************************************

HsCD00038419        CAGGTCTGCACCCACCTGGACGCCCTAAAGTTG
XM_005251788.1      CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
NM_000476.2         CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
XM_005251786.1      CAGGTCTGCACCCACCTGGACGCCCTAAAGTAG
                    *******************************:*

Pathways

Panther : De novo purine biosynthesis
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Synthesis and interconversion of nucleotide di- and triphosphates

Protein

UniProt : Q6FGX9
UniProt : P00568
HPRD : 00046

Clone

Cloning Information : 205286
HIP Master Clone ID : 36358
Original Clone ID : FLH205286.01L

Organism


TAX_ID : 9606
Species Specific ID: 203

Genome

Chromosome : 9
Map Location : 9q34.1
Ensembl : ENSG00000106992

Reagents

Labome : AK1-antibody