DNASU Plasmid Repository • 480.965.5697 | Email

CAMK1 (Homo sapiens) in pJP1563 (retroviral expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CAMK1
Gene Name:  calcium/calmodulin-dependent protein kinase I
Sequence             
Original Clone ID: FLH205292.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038424

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1113nts         Open reading frame : 1 to 1113
Reference Sequence Alignment

ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
GACTTCCGAGATGTTCTGGGCACGGGGGCCTTCTCGGAGGTGATCCTGGCAGAAGATAAG
AGGACGCAGAAGCTGGTGGCCATCAAATGCATTGCCAAGGAGGCCCTGGAGGGCAAGGAA
GGCAGCATGGAGAATGAGATTGCTGTCCTGCACAAGATCAAGCACCCCAACATTGTAGCC
CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
GTGGCTGGGGGGCCGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACA
GAACTGTCCCCCACACTGCCCCACCAGCTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1252
Start on reference sequence 140
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAMK1
Symbol Nomenclature : CAMK1
SYNONYM : CAMKI
Designation : caM kinase I alpha|caM-KI|caMKI-alpha|calcium/calmodulin-dependent protein kinase type 1
Full Nomenclature : calcium/calmodulin-dependent protein kinase I
GENEID : 8536
HGNC : 1459
MIM : 604998
Vega : OTTHUMG00000128419
Target GenBank: NM_003656

 

Reference Sequence Alignment

  

HsCD00038424        ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
NM_003656.4         ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
XM_005265516.1      ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
XM_005265517.1      ATGCTGGGGGCAGTGGAAGGCCCCAGGTGGAAGCAGGCGGAGGACATTAGAGACATCTAC
                    ************************************************************

HsCD00038424        GACTTCCGAGATGTTCTGGGCACGGGGGCCTTCTCGGAGGTGATCCTGGCAGAAGATAAG
NM_003656.4         GACTTCCGAGATGTTCTGGGCACGGGGGCCTTCTCGGAGGTGATCCTGGCAGAAGATAAG
XM_005265516.1      GACTTCCGAGATGTTCTGGGCACGGGGGCCTTCTCGGAGGTGATCCTGGCAGAAGATAAG
XM_005265517.1      GACTTCCGAGATGTTCTGGGCACG------------------------------------
                    ************************                                    

HsCD00038424        AGGACGCAGAAGCTGGTGGCCATCAAATGCATTGCCAAGGAGGCCCTGGAGGGCAAGGAA
NM_003656.4         AGGACGCAGAAGCTGGTGGCCATCAAATGCATTGCCAAGGAGGCCCTGGAGGGCAAGGAA
XM_005265516.1      AGGACGCAGAAGCTGGTGGCCATCAAATGCATTGCCAAGGAGGCCCTGGAGGGCAAGGAA
XM_005265517.1      ------------------------------------------------------------
                                                                                

HsCD00038424        GGCAGCATGGAGAATGAGATTGCTGTCCTGCACAAGATCAAGCACCCCAACATTGTAGCC
NM_003656.4         GGCAGCATGGAGAATGAGATTGCTGTCCTGCACAAGATCAAGCACCCCAACATTGTAGCC
XM_005265516.1      GGCAGCATGGAGAATGAGATTGCTGTCCTGCACAAGATCAAGCACCCCAACATTGTAGCC
XM_005265517.1      ------------------------------------ATCAAGCACCCCAACATTGTAGCC
                                                        ************************

HsCD00038424        CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
NM_003656.4         CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
XM_005265516.1      CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
XM_005265517.1      CTGGATGACATCTATGAGAGTGGGGGCCACCTCTACCTCATCATGCAGCTGGTGTCGGGT
                    ************************************************************

HsCD00038424        GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
NM_003656.4         GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
XM_005265516.1      GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
XM_005265517.1      GGGGAGCTCTTTGACCGTATTGTGGAAAAAGGCTTCTACACGGAGCGGGACGCCAGCCGC
                    ************************************************************

HsCD00038424        CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
NM_003656.4         CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
XM_005265516.1      CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
XM_005265517.1      CTCATCTTCCAGGTGCTGGATGCTGTGAAATACCTGCATGACCTGGGCATTGTACACCGG
                    ************************************************************

HsCD00038424        GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
NM_003656.4         GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
XM_005265516.1      GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
XM_005265517.1      GATCTCAAGCCAGAGAATCTGCTGTACTACAGCCTGGATGAAGACTCCAAAATCATGATC
                    ************************************************************

HsCD00038424        TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
NM_003656.4         TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
XM_005265516.1      TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
XM_005265517.1      TCCGACTTTGGCCTCTCCAAGATGGAGGACCCGGGCAGTGTGCTCTCCACCGCCTGTGGA
                    ************************************************************

HsCD00038424        ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
NM_003656.4         ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
XM_005265516.1      ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
XM_005265517.1      ACTCCGGGATACGTGGCCCCTGAAGTCCTGGCCCAGAAGCCCTACAGCAAGGCTGTGGAT
                    ************************************************************

HsCD00038424        TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
NM_003656.4         TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
XM_005265516.1      TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
XM_005265517.1      TGCTGGTCCATAGGTGTCATCGCCTACATCTTGCTCTGCGGTTACCCTCCCTTCTATGAC
                    ************************************************************

HsCD00038424        GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
NM_003656.4         GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
XM_005265516.1      GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
XM_005265517.1      GAGAATGATGCCAAACTCTTTGAACAGATTTTGAAGGCCGAGTACGAGTTTGACTCTCCT
                    ************************************************************

HsCD00038424        TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
NM_003656.4         TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
XM_005265516.1      TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
XM_005265517.1      TACTGGGACGACATCTCTGACTCTGCCAAAGATTTCATCCGGCACTTGATGGAGAAGGAC
                    ************************************************************

HsCD00038424        CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
NM_003656.4         CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
XM_005265516.1      CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
XM_005265517.1      CCAGAGAAAAGATTCACCTGTGAGCAGGCCTTGCAGCACCCATGGATTGCAGGAGATACA
                    ************************************************************

HsCD00038424        GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
NM_003656.4         GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
XM_005265516.1      GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
XM_005265517.1      GCTCTAGATAAGAATATCCACCAGTCGGTGAGTGAGCAGATCAAGAAGAACTTTGCCAAG
                    ************************************************************

HsCD00038424        AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
NM_003656.4         AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
XM_005265516.1      AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
XM_005265517.1      AGCAAGTGGAAGCAAGCCTTCAATGCCACGGCTGTGGTGCGGCACATGAGGAAACTGCAG
                    ************************************************************

HsCD00038424        CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
NM_003656.4         CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
XM_005265516.1      CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
XM_005265517.1      CTGGGCACCAGCCAGGAGGGGCAGGGGCAGACGGCGAGCCATGGGGAGCTGCTGACACCA
                    ************************************************************

HsCD00038424        GTGGCTGGGGGG------------------------------------------------
NM_003656.4         GTGGCTGGGGGG------------------------------------------------
XM_005265516.1      GTGGCTGGGGGTGAGGAGCGGGCTCTGCAGAAGGGCATGGGTGGTCCACAAAGGTGCACC
XM_005265517.1      GTGGCTGGGGGTGAGGAGCGGGCTCTGCAGAAGGGCATGGGTGGTCCACAAAGGTGCACC
                    ***********                                                 

HsCD00038424        -----------------------------------------------------------C
NM_003656.4         -----------------------------------------------------------C
XM_005265516.1      CGGGCTGGAGTGGAGGGCCTGCCCCTGCGGCCACCTCTGTTCTGTCTTCCCATGCAGGGC
XM_005265517.1      CGGGCTGGAGTGGAGGGCCTGCCCCTGCGGCCACCTCTGTTCTGTCTTCCCATGCAGGGC
                                                                               *

HsCD00038424        CGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACAGAACTGTCCCCCA
NM_003656.4         CGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACAGAACTGTCCCCCA
XM_005265516.1      CGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACAGAACTGTCCCCCA
XM_005265517.1      CGGCAGCTGGCTGTTGCTGTCGAGACTGCTGCGTGGAGCCGGGCACAGAACTGTCCCCCA
                    ************************************************************

HsCD00038424        CACTGCCCCACCAGCTCTTG----------------------
NM_003656.4         CACTGCCCCACCAGCTCTAG----------------------
XM_005265516.1      CACTGCCCCACCAGCTCTAGGGCCCTGGACCTCGGGTCATGA
XM_005265517.1      CACTGCCCCACCAGCTCTAGGGCCCTGGACCTCGGGTCATGA
                    ******************:*                      

Pathways

WikiPathway : Calcium Regulation in the Cardiac Cell

Protein

SMART domain : TyrKc : Tyrosine kinase, catalytic domain
SMART domain : S_TKc : Serine/Threonine protein kinases, catalytic domain
UniProt : B0YIY3
UniProt : Q14012
HPRD : 05412

Clone

Cloning Information : 205292
HIP Master Clone ID : 39686
Original Clone ID : FLH205292.01L

Organism


TAX_ID : 9606
Species Specific ID: 8536

Genome

Chromosome : 3
Map Location : 3p25.3
Ensembl : ENSG00000134072

Reagents

Labome : CAMK1-antibody