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MAP3K11 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  MAP3K11
Gene Name:  mitogen-activated protein kinase kinase kinase 11
Sequence             
Original Clone ID: FLH205293.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038425

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2544nts         Open reading frame : 1 to 2544
Reference Sequence Alignment

ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGC
AGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGT
TATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTG
GCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCCATCTCAGGAGAC
GAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG
TCTCGGGGTGGTGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCTGCGGCTGGAG
GAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG
GTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGC
GTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAG
GCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC
CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAACTGGGCTGTG
CAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCCCGTCATCCACCGT
GATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGACATGGAGCAC
AAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG
AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT
AAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTG
CCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACA
CTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGCCGACTGCTGGGCG
CAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAG
GCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG
CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC
GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGG
CGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTG
CTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGC
AGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC
CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTTCGAGGTCGGG
CCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCCTGCAGAGCCA
GGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG
CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGG
AGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA
GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCC
GAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGGGACGCCCAAGCTG
ATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGGCCTTGGCCGCGAC
CTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACG
CCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC
AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTG
GGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCA
CCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCC
CTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGC
TTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA
GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACCTGCCAACCCC
TTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG
TGGGTGCCGGAAGCGGGGCCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 3037
Start on reference sequence 494
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MAP3K11
Symbol Nomenclature : MAP3K11
SYNONYM : MEKK11; MLK-3; MLK3; PTK1; SPRK
Designation : SH3 domain-containing proline-rich kinase|mixed lineage kinase 3|protein-tyrosine kinase PTK1|src-homology 3 domain-containing proline-rich kinase
Full Nomenclature : mitogen-activated protein kinase kinase kinase 11
GENEID : 4296
HGNC : 6850
MIM : 600050
Vega : OTTHUMG00000166529
Target GenBank: BC011263

 

Reference Sequence Alignment

  

HsCD00038425      ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGC
NM_002419.3       ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGC
                  ************************************************************

HsCD00038425      AGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGT
NM_002419.3       AGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGT
                  ************************************************************

HsCD00038425      TATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTG
NM_002419.3       TATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTG
                  ************************************************************

HsCD00038425      GCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCCATCTCAGGAGAC
NM_002419.3       GCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCCATCTCAGGAGAC
                  ************************************************************

HsCD00038425      GAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG
NM_002419.3       GAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG
                  ************************************************************

HsCD00038425      TCTCGGGGTGGTGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCTGCGGCTGGAG
NM_002419.3       TCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCTGCGGCTGGAG
                  *********** ************************************************

HsCD00038425      GAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG
NM_002419.3       GAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG
                  ************************************************************

HsCD00038425      GTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGC
NM_002419.3       GTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGC
                  ************************************************************

HsCD00038425      GTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAG
NM_002419.3       GTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAG
                  ************************************************************

HsCD00038425      GCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC
NM_002419.3       GCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC
                  ************************************************************

HsCD00038425      CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAACTGGGCTGTG
NM_002419.3       CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAACTGGGCTGTG
                  ************************************************************

HsCD00038425      CAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCCCGTCATCCACCGT
NM_002419.3       CAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCCCGTCATCCACCGT
                  ************************************************************

HsCD00038425      GATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGACATGGAGCAC
NM_002419.3       GATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGACATGGAGCAC
                  ************************************************************

HsCD00038425      AAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG
NM_002419.3       AAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG
                  ************************************************************

HsCD00038425      AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT
NM_002419.3       AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT
                  ************************************************************

HsCD00038425      AAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTG
NM_002419.3       AAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTG
                  ************************************************************

HsCD00038425      CCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACA
NM_002419.3       CCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACA
                  ************************************************************

HsCD00038425      CTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGCCGACTGCTGGGCG
NM_002419.3       CTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGCCGACTGCTGGGCG
                  ************************************************************

HsCD00038425      CAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAG
NM_002419.3       CAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAG
                  ************************************************************

HsCD00038425      GCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG
NM_002419.3       GCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG
                  ************************************************************

HsCD00038425      CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC
NM_002419.3       CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC
                  ************************************************************

HsCD00038425      GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGG
NM_002419.3       GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGG
                  ************************************************************

HsCD00038425      CGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTG
NM_002419.3       CGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTG
                  ************************************************************

HsCD00038425      CTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGC
NM_002419.3       CTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGC
                  ************************************************************

HsCD00038425      AGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC
NM_002419.3       AGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC
                  ************************************************************

HsCD00038425      CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTTCGAGGTCGGG
NM_002419.3       CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTTCGAGGTCGGG
                  ************************************************************

HsCD00038425      CCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCCTGCAGAGCCA
NM_002419.3       CCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCCTGCAGAGCCA
                  ************************************************************

HsCD00038425      GGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG
NM_002419.3       GGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG
                  ************************************************************

HsCD00038425      CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGG
NM_002419.3       CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGG
                  ************************************************************

HsCD00038425      AGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA
NM_002419.3       AGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA
                  ************************************************************

HsCD00038425      GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCC
NM_002419.3       GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCC
                  ************************************************************

HsCD00038425      GAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGGGACGCCCAAGCTG
NM_002419.3       GAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGGGACGCCCAAGCTG
                  ************************************************************

HsCD00038425      ATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGGCCTTGGCCGCGAC
NM_002419.3       ATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGGCCTTGGCCGCGAC
                  ************************************************************

HsCD00038425      CTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACG
NM_002419.3       CTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACG
                  ************************************************************

HsCD00038425      CCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC
NM_002419.3       CCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC
                  ************************************************************

HsCD00038425      AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTG
NM_002419.3       AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTG
                  ************************************************************

HsCD00038425      GGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCA
NM_002419.3       GGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCA
                  ************************************************************

HsCD00038425      CCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCC
NM_002419.3       CCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCC
                  ************************************************************

HsCD00038425      CTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGC
NM_002419.3       CTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGC
                  ************************************************************

HsCD00038425      TTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA
NM_002419.3       TTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA
                  ************************************************************

HsCD00038425      GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACCTGCCAACCCC
NM_002419.3       GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACCTGCCAACCCC
                  ************************************************************

HsCD00038425      TTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG
NM_002419.3       TTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG
                  ************************************************************

HsCD00038425      TGGGTGCCGGAAGCGGGGCCTTTG
NM_002419.3       TGGGTGCCGGAAGCGGGGCCTTGA
                  ********************** .

Pathways

NCI : CDC42 signaling events
NCI : IFN-gamma pathway
NCI : RAC1 signaling pathway
NCI : Reelin signaling pathway
WikiPathway : Insulin Signaling
WikiPathway : MAPK signaling pathway

Clone

Cloning Information : 205293
HIP Master Clone ID : 53115
Original Clone ID : FLH205293.01L

Organism


TAX_ID : 9606
Species Specific ID: 4296

Genome

Chromosome : 11
Map Location : 11q13.1-q13.3
Ensembl : ENSG00000173327

Reagents

Labome : MLK3-antibody