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ARAF1 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  ARAF
Gene Name:  v-raf murine sarcoma 3611 viral oncogene homolog
Sequence             
Original Clone ID: FLH205298.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038429

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1830nts         Open reading frame : 1 to 1830
Reference Sequence Alignment

ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGCCCTCAAGGTTCTACCACAGT
GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
CTACTCAGCGCAGCCCGCCTTGTGCCTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1919
Start on reference sequence 90
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARAF
Symbol Nomenclature : ARAF
SYNONYM : A-RAF; ARAF1; PKS2; RAFA1
Designation : A-Raf proto-oncogene serine/threonine-protein kinase|Oncogene ARAF1|Ras-binding protein DA-Raf|proto-oncogene A-Raf-1|proto-oncogene Pks|serine/threonine-protein kinase A-Raf|v-raf murine sarcoma 3611 viral oncogene homolog 1
Full Nomenclature : v-raf murine sarcoma 3611 viral oncogene homolog
GENEID : 369
Locus Tag : RP1-230G1.1
HGNC : 646
MIM : 311010
Vega : OTTHUMG00000021446
Target GenBank: BC007514

 

Reference Sequence Alignment

  

HsCD00038429        ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
NM_001654.4         ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
NM_001256196.1      ATGGAGCCACCACGGGGCCCCCCTGCCAATGGGGCCGAGCCATCCCGGGCAGTGGGCACC
                    ************************************************************

HsCD00038429        GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
NM_001654.4         GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
NM_001256196.1      GTCAAAGTATACCTGCCCAACAAGCAACGCACGGTGGTGACTGTCCGGGATGGCATGAGT
                    ************************************************************

HsCD00038429        GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
NM_001654.4         GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
NM_001256196.1      GTCTACGACTCTCTAGACAAGGCCCTGAAGGTGCGGGGTCTAAATCAGGACTGCTGTGTG
                    ************************************************************

HsCD00038429        GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
NM_001654.4         GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
NM_001256196.1      GTCTACCGACTCATCAAGGGACGAAAGACGGTCACTGCCTGGGACACAGCCATTGCTCCC
                    ************************************************************

HsCD00038429        CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
NM_001654.4         CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
NM_001256196.1      CTGGATGGCGAGGAGCTCATTGTCGAGGTCCTTGAAGATGTCCCGCTGACCATGCACAAT
                    ************************************************************

HsCD00038429        TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
NM_001654.4         TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
NM_001256196.1      TTTGTACGGAAGACCTTCTTCAGCCTGGCGTTCTGTGACTTCTGCCTTAAGTTTCTGTTC
                    ************************************************************

HsCD00038429        CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
NM_001654.4         CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
NM_001256196.1      CATGGCTTCCGTTGCCAAACCTGTGGCTACAAGTTCCACCAGCATTGTTCCTCCAAGGTC
                    ************************************************************

HsCD00038429        CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGCCCTCAAGGTTCTACCACAGT
NM_001654.4         CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAG---------TTCTACCACAGT
NM_001256196.1      CCCACAGTCTGTGTTGACATGAGTACCAACCGCCAACAGCCCTCAAGGTTCTACCACAGT
                    ***************************************         ************

HsCD00038429        GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
NM_001654.4         GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
NM_001256196.1      GTCCAGGATTTGTCCGGAGGCTCCAGACAGCATGAGGCTCCCTCGAACCGCCCCCTGAAT
                    ************************************************************

HsCD00038429        GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
NM_001654.4         GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
NM_001256196.1      GAGTTGCTAACCCCCCAGGGTCCCAGCCCCCGCACCCAGCACTGTGACCCGGAGCACTTC
                    ************************************************************

HsCD00038429        CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
NM_001654.4         CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
NM_001256196.1      CCCTTCCCTGCCCCAGCCAATGCCCCCCTACAGCGCATCCGCTCCACGTCCACTCCCAAC
                    ************************************************************

HsCD00038429        GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
NM_001654.4         GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
NM_001256196.1      GTCCATATGGTCAGCACCACGGCCCCCATGGACTCCAACCTCATCCAGCTCACTGGCCAG
                    ************************************************************

HsCD00038429        AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
NM_001654.4         AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
NM_001256196.1      AGTTTCAGCACTGATGCTGCCGGTAGTAGAGGAGGTAGTGATGGAACCCCCCGGGGGAGC
                    ************************************************************

HsCD00038429        CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
NM_001654.4         CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
NM_001256196.1      CCCAGCCCAGCCAGCGTGTCCTCGGGGAGGAAGTCCCCACATTCCAAGTCACCAGCAGAG
                    ************************************************************

HsCD00038429        CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
NM_001654.4         CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
NM_001256196.1      CAGCGCGAGCGGAAGTCCTTGGCCGATGACAAGAAGAAAGTGAAGAACCTGGGGTACCGG
                    ************************************************************

HsCD00038429        GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
NM_001654.4         GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
NM_001256196.1      GACTCAGGCTATTACTGGGAGGTACCACCCAGTGAGGTGCAGCTGCTGAAGAGGATCGGG
                    ************************************************************

HsCD00038429        ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
NM_001654.4         ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
NM_001256196.1      ACGGGCTCGTTTGGCACCGTGTTTCGAGGGCGGTGGCATGGCGATGTGGCCGTGAAGGTG
                    ************************************************************

HsCD00038429        CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
NM_001654.4         CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
NM_001256196.1      CTCAAGGTGTCCCAGCCCACAGCTGAGCAGGCCCAGGCTTTCAAGAATGAGATGCAGGTG
                    ************************************************************

HsCD00038429        CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
NM_001654.4         CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
NM_001256196.1      CTCAGGAAGACGCGACATGTCAACATCTTGCTGTTTATGGGCTTCATGACCCGGCCGGGA
                    ************************************************************

HsCD00038429        TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
NM_001654.4         TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
NM_001256196.1      TTTGCCATCATCACACAGTGGTGTGAGGGCTCCAGCCTCTACCATCACCTGCATGTGGCC
                    ************************************************************

HsCD00038429        GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
NM_001654.4         GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
NM_001256196.1      GACACACGCTTCGACATGGTCCAGCTCATCGACGTGGCCCGGCAGACTGCCCAGGGCATG
                    ************************************************************

HsCD00038429        GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
NM_001654.4         GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
NM_001256196.1      GACTACCTCCATGCCAAGAACATCATCCACCGAGATCTCAAGTCTAACAACATCTTCCTA
                    ************************************************************

HsCD00038429        CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
NM_001654.4         CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
NM_001256196.1      CATGAGGGGCTCACGGTGAAGATCGGTGACTTTGGCTTGGCCACAGTGAAGACTCGATGG
                    ************************************************************

HsCD00038429        AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
NM_001654.4         AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
NM_001256196.1      AGCGGGGCCCAGCCCTTGGAGCAGCCCTCAGGATCTGTGCTGTGGATGGCAGCTGAGGTG
                    ************************************************************

HsCD00038429        ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
NM_001654.4         ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
NM_001256196.1      ATCCGTATGCAGGACCCGAACCCCTACAGCTTCCAGTCAGACGTCTATGCCTACGGGGTT
                    ************************************************************

HsCD00038429        GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
NM_001654.4         GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
NM_001256196.1      GTGCTCTACGAGCTTATGACTGGCTCACTGCCTTACAGCCACATTGGCTGCCGTGACCAG
                    ************************************************************

HsCD00038429        ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
NM_001654.4         ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
NM_001256196.1      ATTATCTTTATGGTGGGCCGTGGCTATCTGTCCCCGGACCTCAGCAAAATCTCCAGCAAC
                    ************************************************************

HsCD00038429        TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
NM_001654.4         TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
NM_001256196.1      TGCCCCAAGGCCATGCGGCGCCTGCTGTCTGACTGCCTCAAGTTCCAGCGGGAGGAGCGG
                    ************************************************************

HsCD00038429        CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
NM_001654.4         CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
NM_001256196.1      CCCCTCTTCCCCCAGATCCTGGCCACAATTGAGCTGCTGCAACGGTCACTCCCCAAGATT
                    ************************************************************

HsCD00038429        GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
NM_001654.4         GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
NM_001256196.1      GAGCGGAGTGCCTCGGAACCCTCCTTGCACCGCACCCAGGCCGATGAGTTGCCTGCCTGC
                    ************************************************************

HsCD00038429        CTACTCAGCGCAGCCCGCCTTGTGCCTTTG
NM_001654.4         CTACTCAGCGCAGCCCGCCTTGTGCCTTAG
NM_001256196.1      CTACTCAGCGCAGCCCGCCTTGTGCCTTAG
                    ****************************:*

Pathways

Panther : Angiogenesis
Panther : B cell activation
Panther : Integrin signalling pathway
Panther : Interleukin signaling pathway
Panther : PDGF signaling pathway
Panther : Ras Pathway
Panther : T cell activation
Panther : VEGF signaling pathway
WikiPathway : ErbB signaling pathway
WikiPathway : Focal Adhesion
WikiPathway : Integrated Breast Cancer Pathway
WikiPathway : Integrin-mediated cell adhesion
WikiPathway : MAPK Cascade

Clone

Cloning Information : 205298
HIP Master Clone ID : 61557
Original Clone ID : FLH205298.01L

Organism


TAX_ID : 9606
Species Specific ID: 369

Genome

Chromosome : X
Map Location : Xp11.4-p11.2
Ensembl : ENSG00000078061

Reagents

Labome : ARAF-antibody