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CHKA (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CHKA
Gene Name:  choline kinase alpha
Sequence             
Original Clone ID: FLH205319.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038443

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1233nts         Open reading frame : 1 to 1233
Reference Sequence Alignment

ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTG
CCGCTGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGACGAGCAGCCGGAGCCCCGGACG
CGGCGCAGGGCCTATCTGTGGTGCAAGGAGTTCCTGCCCGGCGCCTGGCGGGGCCTCCGC
GAGGACGAGTTCCACATCAGTGTCATCAGAGGCGGCCTTAGCAACATGCTGTTCCAGTGC
TCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCTCGGAAAGTGCTCCTGCGGCTG
TATGGAGCGATTTTGCAGATGAGGTCCTGTAATAAAGAGGGATCCGAACAAGCTCAGAAA
GAAAATGAATTTCAAGGGGCTGAGGCCATGGTTCTGGAGAGCGTTATGTTTGCCATTCTC
GCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTTCCCCAAGGCCGACTGGAGCAG
TTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAAGTTTGCCAGATATTTCTGCAGAA
ATCGCCGAGAAAATGGCTACATTTCATGGTATGAAAATGCCATTCAATAAGGAACCAAAA
TGGCTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTGCTGAGAATTAAATTTACTGAG
GAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTACAATCTGCCCTTGGAACTGGAA
AACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTTGTATTTTGTCATAATGACTGT
CAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAATTCTGAAAAACAGAAACTGATG
CTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGATTCGACATTGGAAATCACTTC
TGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCTTTTTTCAGAGCAAACATCCGG
AAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCCAGTTACTTGCCTGCATTCCAA
AATGACTTTGAAAACCTCAGTACTGAAGAAAAATCCATTATAAAAGAAGAAATGTTGCTT
GAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGGGGACTGTGGTCCATTGTACAA
GCCAAGATTTCATCTATTGAATTTGGGTACATGGACTACGCCCAAGCAAGGTTTGATGCC
TATTTCCACCAGAAGAGGAAGCTTGGGGTGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1588
Start on reference sequence 215
Mutations:
None
Discrepancy:
del@57,141;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CHKA
Symbol Nomenclature : CHKA
SYNONYM : CHK; CK; CKI; EK
Designation : CHETK-alpha|ethanolamine kinase
Full Nomenclature : choline kinase alpha
GENEID : 1119
HGNC : 1937
MIM : 118491
Vega : OTTHUMG00000167424
Target GenBank: NM_001277

 

Reference Sequence Alignment

  

HsCD00038443      ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGG----------
NM_001277.2       ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTG
NM_212469.1       ATGAAAACCAAATTCTGCACCGGGGGCGAGGCGGAGCCCTCGCCGCTCGGGCTGCTGCTG
                  **************************************************          

HsCD00038443      ------------------------------------------------------------
NM_001277.2       AGCTGCGGTAGCGGCAGCGCGGCCCCGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCC
NM_212469.1       AGCTGCGGTAGCGGCAGCGCGGCCCCGGCGCCCGGCGTGGGGCAGCAGCGCGACGCCGCC
                                                                              

HsCD00038443      ------------------------------------------------------------
NM_001277.2       AGCGACCTCGAGTCCAAGCAGCTGGGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCG
NM_212469.1       AGCGACCTCGAGTCCAAGCAGCTGGGCGGCCAACAGCCGCCGCTCGCGCTGCCCCCTCCG
                                                                              

HsCD00038443      -----------GCTGCTGCTGCCGCTGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGAC
NM_001277.2       CCGCCGCTGCCGCTGCCGCTGCCGCTGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGAC
NM_212469.1       CCGCCGCTGCCGCTGCCGCTGCCGCTGCCCCAGCCCCCGCCGCCGCAGCCGCCCGCAGAC
                             ***** *******************************************

HsCD00038443      GAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGTGGTGCAAGGAGTTCCTGCCC
NM_001277.2       GAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGTGGTGCAAGGAGTTCCTGCCC
NM_212469.1       GAGCAGCCGGAGCCCCGGACGCGGCGCAGGGCCTATCTGTGGTGCAAGGAGTTCCTGCCC
                  ************************************************************

HsCD00038443      GGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAGAGGCGGCCTT
NM_001277.2       GGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAGAGGCGGCCTT
NM_212469.1       GGCGCCTGGCGGGGCCTCCGCGAGGACGAGTTCCACATCAGTGTCATCAGAGGCGGCCTT
                  ************************************************************

HsCD00038443      AGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
NM_001277.2       AGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
NM_212469.1       AGCAACATGCTGTTCCAGTGCTCCCTACCTGACACCACAGCCACCCTTGGTGATGAGCCT
                  ************************************************************

HsCD00038443      CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGATGAGGTCCTGTAATAAAGAG
NM_001277.2       CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGATGAGGTCCTGTAATAAAGAG
NM_212469.1       CGGAAAGTGCTCCTGCGGCTGTATGGAGCGATTTTGCAGATGGG----------------
                  ******************************************.*                

HsCD00038443      GGATCCGAACAAGCTCAGAAAGAAAATGAATTTCAAGGGGCTGAGGCCATGGTTCTGGAG
NM_001277.2       GGATCCGAACAAGCTCAGAAAGAAAATGAATTTCAAGGGGCTGAGGCCATGGTTCTGGAG
NM_212469.1       --------------------------------------GGCTGAGGCCATGGTTCTGGAG
                                                        **********************

HsCD00038443      AGCGTTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTT
NM_001277.2       AGCGTTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTT
NM_212469.1       AGCGTTATGTTTGCCATTCTCGCAGAGAGGTCACTTGGGCCAAAACTCTATGGCATCTTT
                  ************************************************************

HsCD00038443      CCCCAAGGCCGACTGGAGCAGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAAGT
NM_001277.2       CCCCAAGGCCGACTGGAGCAGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAAGT
NM_212469.1       CCCCAAGGCCGACTGGAGCAGTTCATCCCGAGCCGGCGATTAGATACTGAAGAATTAAGT
                  ************************************************************

HsCD00038443      TTGCCAGATATTTCTGCAGAAATCGCCGAGAAAATGGCTACATTTCATGGTATGAAAATG
NM_001277.2       TTGCCAGATATTTCTGCAGAAATCGCCGAGAAAATGGCTACATTTCATGGTATGAAAATG
NM_212469.1       TTGCCAGATATTTCTGCAGAAATCGCCGAGAAAATGGCTACATTTCATGGTATGAAAATG
                  ************************************************************

HsCD00038443      CCATTCAATAAGGAACCAAAATGGCTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTG
NM_001277.2       CCATTCAATAAGGAACCAAAATGGCTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTG
NM_212469.1       CCATTCAATAAGGAACCAAAATGGCTTTTTGGCACAATGGAAAAGTATCTAAAGGAAGTG
                  ************************************************************

HsCD00038443      CTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTAC
NM_001277.2       CTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTAC
NM_212469.1       CTGAGAATTAAATTTACTGAGGAATCCAGAATTAAAAAGCTCCACAAATTGCTCAGTTAC
                  ************************************************************

HsCD00038443      AATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTT
NM_001277.2       AATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTT
NM_212469.1       AATCTGCCCTTGGAACTGGAAAACCTGAGATCATTGCTTGAATCTACTCCATCTCCAGTT
                  ************************************************************

HsCD00038443      GTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAAT
NM_001277.2       GTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAAT
NM_212469.1       GTATTTTGTCATAATGACTGTCAAGAAGGTAATATCTTGTTGCTGGAAGGCCGAGAGAAT
                  ************************************************************

HsCD00038443      TCTGAAAAACAGAAACTGATGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGA
NM_001277.2       TCTGAAAAACAGAAACTGATGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGA
NM_212469.1       TCTGAAAAACAGAAACTGATGCTCATTGATTTCGAATACAGCAGTTACAATTACAGGGGA
                  ************************************************************

HsCD00038443      TTCGACATTGGAAATCACTTCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCT
NM_001277.2       TTCGACATTGGAAATCACTTCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCT
NM_212469.1       TTCGACATTGGAAATCACTTCTGTGAGTGGATGTATGATTATAGCTATGAAAAATACCCT
                  ************************************************************

HsCD00038443      TTTTTCAGAGCAAACATCCGGAAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCC
NM_001277.2       TTTTTCAGAGCAAACATCCGGAAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCC
NM_212469.1       TTTTTCAGAGCAAACATCCGGAAGTATCCCACCAAGAAACAACAGCTCCATTTTATTTCC
                  ************************************************************

HsCD00038443      AGTTACTTGCCTGCATTCCAAAATGACTTTGAAAACCTCAGTACTGAAGAAAAATCCATT
NM_001277.2       AGTTACTTGCCTGCATTCCAAAATGACTTTGAAAACCTCAGTACTGAAGAAAAATCCATT
NM_212469.1       AGTTACTTGCCTGCATTCCAAAATGACTTTGAAAACCTCAGTACTGAAGAAAAATCCATT
                  ************************************************************

HsCD00038443      ATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGG
NM_001277.2       ATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGG
NM_212469.1       ATAAAAGAAGAAATGTTGCTTGAAGTTAATAGGTTTGCCCTTGCATCTCATTTCCTCTGG
                  ************************************************************

HsCD00038443      GGACTGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATGGACTAC
NM_001277.2       GGACTGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATGGACTAC
NM_212469.1       GGACTGTGGTCCATTGTACAAGCCAAGATTTCATCTATTGAATTTGGGTACATGGACTAC
                  ************************************************************

HsCD00038443      GCCCAAGCAAGGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTGTTG
NM_001277.2       GCCCAAGCAAGGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTGTGA
NM_212469.1       GCCCAAGCAAGGTTTGATGCCTATTTCCACCAGAAGAGGAAGCTTGGGGTGTGA
                  **************************************************** .

Pathways

Reactome : Glycerophospholipid biosynthesis
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Phospholipid metabolism
Reactome : Synthesis of PC
Reactome : Synthesis of PE
WikiPathway : Acetylcholine Synthesis

Protein

UniProt : P35790
HPRD : 00327

Clone

Cloning Information : 205319
HIP Master Clone ID : 177834
Original Clone ID : FLH205319.01L

Organism


TAX_ID : 9606
Species Specific ID: 1119

Genome

Chromosome : 11
Map Location : 11q13.2
Ensembl : ENSG00000110721

Reagents

Labome : CHKA-antibody