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PSPTO_2317 (P. syringae pv. tomato str. DC3000 ) in pSGX3

TOP

Explanation of Terms


Gene: Gene Symbol:  PSPTO_2317
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: 11221c4BCt8p1
Keyword: None
Species: Pseudomonas syringae pv. tomato str. DC3000
Type: cDNA
Vector Name: pSGX3               Format:  CLOSED
Source: New York SGX Research Center for Structural Genomics
Description: None
Comments: Linker not verified. Actual 5' flanking sequence provided below.
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
New York SGX Research Center for Structural Genomics
PsCD00385788

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1623nts         Open reading frame : 51 to 1673

TCCTTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTCAGAAATTGG
CTTTTTCTTACGTTCGGTTTTCCAGTCTTAAGCAACGCCATGGCGATAGCCTCAAGCGTC
AGACGGACATGGTTGCAAATTGGCTACTGGCAAACCCTGAATACAAGTTAAGCGGGGAGA
CTAGCTACCAAGACCTGGGCAAATCAGGCTTTAGTGGTGCGCACCTCGACAATGCTTTCG
GTCGTCTTTTAGCAGCGATAGAAAACGGCGCTATCCCTAAAGGCTCTGCCATCCTGATTG
AACAGATAGACCGTGCAGGACGTATGGAGCCAATGGAAATGCTCCCTCTACTCTCCCGCA
TTGTGAACGCTGGTGTTGACCTTGTGACACTGGACGACGGAATCACATATGACAGAGCAT
CAGTGAACAGTAATCACTTGTTCCTGCTCGTCGCCAAGGTACAGCAGGCACACCAGTATT
CTGACTCCCTAAGCCGTCGCCTTAGAGCTGCCTATCAGACCAAGCGAGCTAAAGCCGCTG
CTGGGGAAGGTGCTACCAGACGTATGCCACTTTGGATTCGTGACGGAAAGTTGCGTGACG
ATATCGCCCCATTCGTCACTCAGGTTTTTGAAGACTACGCGGCGGGTATTGGTGAGCGTC
GAATTCTTGGAAGGCTCAAAGGACAACATCCAGACTTCGAAACGATCAGTCCTTCGACCT
TCCGCCGCTGGCTGAGAAACCCTGTGGCCGTTGGACGCTGGAACGACATTGAAGACGTAT
GGCCCGCCGTGGTGAGTAAAGAGCTTTGGTTTCGTGTACAGAAGCGACTGACTGATGGTG
TTAAACCCAAAGCTGCCTCATCCAAATATTTGCTATCAGGTTTGGTTAAGTGTGCTCGGT
GCGGTAAAAACTACTGTGTTGGCGGACAAAAAGGAGCATTGACGATGCTCTGTGTGAGCC
GCCACAGGTTTGGTTCTGGCGCAGGTGGTTGCACGAATAAACGATCCATCCCCTACGCCG
TGTTTGACTTCATCAGGTCACAGACGGCACATGCCGCATTAGTAAGGGCATCACAAAGCC
AGAATTTGACAGCAAGTGAAAAGCGTCAGATTGAAATTGACGGAGAGCTAATCGAGTTGC
GTAAGCAGTCATCACATGCGGCTGAAGGTTTGGTGCGCTATGGGATGGTGAAGGAAATCA
CGGATGTTCTTGATCGTGTCACAGACCAGATTGACCGGTTGGAGAAAGAGCGTGTAATGC
TCCAGGCAGTGCCAGCCCCAGCAACGCTTGACGACATGATAGACGTGGAGAATGACCTAC
TTGATGATGATATCGTGAAGCTGAATGCGCTTTTGCAAGGCGTAGGCTACGAGATGGTTG
TGGATGATCGTACAATCGTTGTGTGCGAAGCCAGCGTATTCAGCACGCTTCAAAGTCAAA
CTTATAGATACATTGGTGCGGACCGTGTCACTGGTACTTATAAAGTGACTGCTAACGATG
AAGTGCTCCATCACCTGCCTAATCTTGATTCTAAGGTGCAGCAAGATGAGTACGCAGAAT
TTCTGAAGAAAGAGGCTCGTTACGAGTCTGGCGAGCTTAAGCGAGACGAGTTTAGGTTTA
ATCTTGAGACGGGCGAAATCAATCGCGAAGGGCATCATCACCATCACCACTGATCCGAAT
TCGAGCTCCG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGTCCCTT

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11221c
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSGX3

pSGX3 in advanced viewed

Synonyms: pSGX-3, pSGX3_BC
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments: Modified from pET26b from Novagen
Size (bp): 5339
Parent Vector: None
Empty Vector: None
Properties: TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name: New York SGX Research Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start start initiation start (Met, Ser, Leu) 5072 5079
TOPO recognition site TOPO TOPO cloning site 5076 5085
bacterial origin ori pBR322-type bacterial origin of replication 1421 2103
gene fragment ROP ROP 2527 2703
promoter T7 T7 promoter 4983 5003
repressor protein gene LacI LacI CDS 3515 4531
selectable marker KanR kanamycin resistance gene 563 1375
tag His 6xHis tag 5086 5103
trxn regulatory element lac operator lac operator 5003 5025
trxn termination sequence T7 term T7 terminator 5231 5307

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : PSPTO_2317
SYNONYM : PSPTO2317
GENEID : 1183968
Locus Tag : PSPTO_2317
GI : 28852759
GenBank Accession : AAO55831.1
Target GenBank: None

 

Protein

SMART domain : Resolvase : Resolvase, N terminal domain
Structural Annotation Wiki : TOPSAN
UniProt : Q883N3

Clone

Cloning Information : 552112
HIP Master Clone ID : 462432
Original Clone ID : 11221c4BCt8p1

Experimental

TargetTrack - Experimental data : NYSGXRC-11221c

Organism


TAX_ID : 223283
Species Specific ID: 1183968

Reagents

Labome : PSPTO_2317-cDNA