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IRAK2 (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  IRAK2
Gene Name:  interleukin-1 receptor-associated kinase 2
Sequence             
Original Clone ID: FLH205504.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038585

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1773nts         Open reading frame : 1 to 1773
Reference Sequence Alignment

ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
GCCCCTCATTCGTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
CCGGTTTACCTGAAGGACTTACTCCTCAGTGAAATTCCAAGCAGCACCGCCTCGCTCTGC
TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
GTTGGCCTGGAGCCTCCCCAGGATGTTACATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1782
Start on reference sequence 10
Mutations:
None
Discrepancy:
c492g; t1293a,D431E
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IRAK2
Symbol Nomenclature : IRAK2
SYNONYM : IRAK-2
Designation : interleukin-1 receptor associated kinase-2|interleukin-1 receptor-associated kinase-like 2
Full Nomenclature : interleukin-1 receptor-associated kinase 2
GENEID : 3656
HGNC : 6113
MIM : 603304
Vega : OTTHUMG00000155358
Target GenBank: NM_001570

 

Reference Sequence Alignment

  

HsCD00038585      ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
NM_001570.3       ATGGCCTGCTACATCTACCAGCTGCCCTCCTGGGTGCTGGACGACCTGTGCCGCAACATG
                  ************************************************************

HsCD00038585      GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
NM_001570.3       GACGCGCTCAGCGAGTGGGACTGGATGGAGTTCGCCTCCTACGTGATCACAGACCTGACC
                  ************************************************************

HsCD00038585      CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
NM_001570.3       CAGCTGCGGAAGATCAAGTCCATGGAGCGGGTGCAGGGTGTGAGCATCACGCGGGAGCTG
                  ************************************************************

HsCD00038585      CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
NM_001570.3       CTGTGGTGGTGGGGCATGCGGCAGGCCACCGTCCAGCAACTTGTGGACCTCCTGTGCCGC
                  ************************************************************

HsCD00038585      CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
NM_001570.3       CTGGAGCTCTACCGGGCTGCCCAGATCATCCTGAACTGGAAACCGGCTCCTGAAATCAGG
                  ************************************************************

HsCD00038585      TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
NM_001570.3       TGTCCCATTCCAGCCTTCCCTGACTCTGTGAAGCCAGAAAAGCCTTTGGCAGCTTCTGTA
                  ************************************************************

HsCD00038585      AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
NM_001570.3       AGAAAGGCTGAGGATGAACAGGAAGAGGGGCAGCCTGTGAGGATGGCCACCTTTCCAGGC
                  ************************************************************

HsCD00038585      CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
NM_001570.3       CCAGGGTCCTCTCCAGCCAGAGCCCACCAGCCGGCCTTTCTCCAGCCTCCTGAAGAAGAT
                  ************************************************************

HsCD00038585      GCCCCTCATTCGTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
NM_001570.3       GCCCCTCATTCCTTGAGAAGCGACCTCCCCACTTCGTCTGATTCAAAGGACTTCAGCACC
                  *********** ************************************************

HsCD00038585      TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
NM_001570.3       TCCATTCCTAAGCAGGAAAAACTTTTGAGCTTGGCTGGAGACAGCCTTTTCTGGAGTGAG
                  ************************************************************

HsCD00038585      GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
NM_001570.3       GCAGACGTGGTCCAGGCAACCGATGACTTCAATCAAAACCGCAAAATCAGCCAGGGGACC
                  ************************************************************

HsCD00038585      TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
NM_001570.3       TTTGCTGACGTCTACAGAGGGCACAGGCACGGGAAGCCATTCGTCTTCAAGAAGCTCAGA
                  ************************************************************

HsCD00038585      GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
NM_001570.3       GAGACAGCCTGTTCAAGTCCAGGATCAATCGAAAGATTCTTCCAGGCAGAGTTGCAGATT
                  ************************************************************

HsCD00038585      TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
NM_001570.3       TGTCTTAGATGCTGCCACCCCAATGTCTTACCTGTGCTGGGCTTCTGTGCTGCAAGACAG
                  ************************************************************

HsCD00038585      TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
NM_001570.3       TTTCACAGCTTCATCTACCCCTACATGGCAAATGGTTCCCTACAGGACAGACTGCAGGGT
                  ************************************************************

HsCD00038585      CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
NM_001570.3       CAGGGTGGCTCGGACCCCCTCCCCTGGCCCCAGCGTGTCAGCATCTGCTCAGGGCTGCTC
                  ************************************************************

HsCD00038585      TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
NM_001570.3       TGTGCCGTCGAGTACCTGCATGGTCTGGAGATCATCCACAGCAACGTCAAGAGCTCTAAT
                  ************************************************************

HsCD00038585      GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
NM_001570.3       GTCTTGCTGGACCAAAATCTCACCCCCAAACTTGCTCACCCAATGGCTCATCTGTGTCCT
                  ************************************************************

HsCD00038585      GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
NM_001570.3       GTCAACAAAAGGTCAAAATACACCATGATGAAGACTCACCTGCTCCGGACGTCAGCCGCG
                  ************************************************************

HsCD00038585      TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
NM_001570.3       TATCTGCCAGAGGATTTCATCCGGGTGGGGCAGCTGACAAAGCGAGTGGACATCTTCAGC
                  ************************************************************

HsCD00038585      TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
NM_001570.3       TGTGGAATAGTGTTGGCCGAGGTCCTCACGGGCATCCCTGCAATGGATAACAACCGAAGC
                  ************************************************************

HsCD00038585      CCGGTTTACCTGAAGGACTTACTCCTCAGTGAAATTCCAAGCAGCACCGCCTCGCTCTGC
NM_001570.3       CCGGTTTACCTGAAGGACTTACTCCTCAGTGATATTCCAAGCAGCACCGCCTCGCTCTGC
                  ********************************:***************************

HsCD00038585      TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
NM_001570.3       TCCAGGAAGACGGGCGTGGAGAACGTGATGGCAAAGGAGATCTGCCAGAAGTACCTGGAG
                  ************************************************************

HsCD00038585      AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
NM_001570.3       AAGGGCGCAGGGAGGCTTCCGGAGGACTGCGCCGAGGCCCTGGCCACGGCTGCCTGCCTG
                  ************************************************************

HsCD00038585      TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
NM_001570.3       TGCCTGCGGAGGCGTAACACCAGCCTGCAGGAGGTGTGTGGCTCTGTGGCTGCTGTGGAA
                  ************************************************************

HsCD00038585      GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
NM_001570.3       GAGCGGCTCCGAGGTCGGGAGACGTTGCTCCCTTGGAGTGGGCTTTCTGAGGGTACAGGC
                  ************************************************************

HsCD00038585      TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
NM_001570.3       TCTTCTTCCAACACCCCAGAGGAAACAGACGACGTTGACAATTCCAGCCTTGATGCCTCC
                  ************************************************************

HsCD00038585      TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
NM_001570.3       TCCTCCATGAGTGTGGCACCCTGGGCAGGGGCTGCCACCCCACTTCTCCCCACAGAGAAT
                  ************************************************************

HsCD00038585      GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
NM_001570.3       GGGGAAGGAAGGCTGCGGGTCATCGTGGGAAGGGAGGCTGACTCCTCCTCTGAGGCCTGT
                  ************************************************************

HsCD00038585      GTTGGCCTGGAGCCTCCCCAGGATGTTACATTG---------------------------
NM_001570.3       GTTGGCCTGGAGCCTCCCCAGGATGTTACAGAAACTTCGTGGCAAATTGAGATCAATGAG
                  ****************************** :.                           

HsCD00038585      ------------------------------------------------------------
NM_001570.3       GCCAAAAGGAAACTGATGGAGAATATTCTGCTCTACAAAGAGGAAAAAGTGGACAGCATT
                                                                              

HsCD00038585      ------------------
NM_001570.3       GAGCTCTTTGGCCCCTGA
                                    

Pathways

NCI : Endogenous TLR signaling
Reactome : Activated TLR4 signalling
Reactome : Cytokine Signaling in Immune system
Reactome : IRAK2 mediated activation of TAK1 complex
Reactome : IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interleukin-1 signaling
Reactome : JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1
Reactome : MAP kinase activation in TLR cascade
Reactome : MyD88 cascade initiated on plasma membrane
Reactome : MyD88 dependent cascade initiated on endosome
Reactome : MyD88-independent cascade
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : NOD1/2 Signaling Pathway
Reactome : Nucleotide-binding domain, leucine rich repeat containing receptor (NLR) signaling pathways
Reactome : Signaling by Interleukins
Reactome : TAK1 activates NFkB by phosphorylation and activation of IKKs complex
Reactome : TRAF6 Mediated Induction of proinflammatory cytokines
Reactome : TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
Reactome : TRIF-mediated TLR3/TLR4 signaling
Reactome : Toll Like Receptor 10 (TLR10) Cascade
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 3 (TLR3) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor 5 (TLR5) Cascade
Reactome : Toll Like Receptor 7/8 (TLR7/8) Cascade
Reactome : Toll Like Receptor 9 (TLR9) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
Reactome : activated TAK1 mediates p38 MAPK activation
WikiPathway : IL-1 signaling pathway
WikiPathway : Regulation of toll-like receptor signaling pathway

Clone

Cloning Information : 205504
HIP Master Clone ID : 148036
Original Clone ID : FLH205504.01L

Organism


TAX_ID : 9606
Species Specific ID: 3656

Genome

Chromosome : 3
Map Location : 3p25.3
Ensembl : ENSG00000134070

Reagents

Labome : IRAK2-antibody