DNASU Plasmid Repository • 480.965.5697 | Email

STK24 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  STK24
Gene Name:  serine/threonine kinase 24
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH021500.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00000386

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1296nts         Open reading frame : 1 to 1296
Reference Sequence Alignment

ATGGCTCACTCCCCGGTGCAGTCGGGCCTGCCCGGCATGCAGAACCTAAAGGCAGACCCA
GAAGAGCTTTTTACAAAACTAGAGAAAATTGGGAAGGGCTCCTTTGGAGAGGTGTTCAAA
GGCATTGACAATCGGACTCAGAAAGTGGTTGCCATAAAGATCATTGATCTGGAAGAAGCT
GAAGATGAGATAGAGGACATTCAACAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCA
TATGTAACCAAATATTATGGATCCTATCTGAAGGATACAAAATTATGGATAATAATGGAA
TATCTTGGTGGAGGCTCCGCACTAGATCTATTAGAACCTGGCCCATTAGATGAAACCCAG
ATCGCTACTATATTAAGAGAAATACTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAA
ATCCACAGAGACATTAAAGCGGCCAACGTCCTGCTGTCTGAGCATGGCGAGGTGAAGCTG
GCGGACTTTGGCGTGGCTGGCCAGCTGACAGACACCCAGATCAAAAGGAACACCTTCGTG
GGCACCCCATTCTGGATGGCACCCGAGGTCATCAAACAGTCGGCCTATGACTCGAAGGCA
GACATCTGGTCCCTGGGCATAACAGCTATTGAACTTGCAAGAGGGGAACCACCTCATTCC
GAGCTGCACCCCATGAAAGTTTTATTCCTCATTCCAAAGAACAACCCACCGACGTTGGAA
GGAAACTACAGTAAACCCCTCAAGGAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGC
TTTAGACCCACTGCTAAGGAGTTATTGAAGCACAAGTTTATACTACGCAATGCAAAGAAA
ACTTCCTACTTGACCGAGCTCATCGACAGGTACAAGAGATGGAAGGCCGAGCAGAGCCAT
GACGACTCGAGCTCCGAGGATTCCGACGCGGAAACAGATGGCCAAGCCTCGGGGGGCAGT
GATTCTGGGGACTGGATCTTCACAATCCGAGAAAAAGATCCCAAGAATCTCGAGAATGGA
GCTCTTCAGCCATCGGACTTGGACAGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTC
TCTCAGTGTTTATCTACAATTATTTCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAG
GCGTGCGGAGGGAACTTGGGGTCCATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAG
GAGGCGTGCCCTGGCATCTCCGACACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGA
TACTCTCTAAGTGGTGGAGGAACTTCATCCCACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1374
Start on reference sequence 79
Mutations:
None
Discrepancy:
t1161g; t1205c,V402A
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : STK24
Symbol Nomenclature : STK24
SYNONYM : HEL-S-95; MST3; MST3B; STE20; STK3
Designation : STE20-like kinase 3|STE20-like kinase MST3|epididymis secretory protein Li 95|mammalian STE20-like protein kinase 3|serine/threonine kinase 24 (STE20 homolog, yeast)|serine/threonine-protein kinase 24|sterile 20-like kinase 3
Full Nomenclature : serine/threonine kinase 24
GENEID : 8428
Locus Tag : RP11-111L24.5
GI : 61355569
GenBank Accession : AY888206
HGNC : 11403
MIM : 604984
Vega : OTTHUMG00000017250
Target GenBank: NM_003576

 

Reference Sequence Alignment

  

HsCD00000386        ------------------------------------ATGGCTCACTCCCCGGTGCAGTCG
NM_003576.4         ATGGACTCCAGAGCCCAGCTTTGGGGACTGGCCTTGAATAAAAGGAGGGCCACTCTACCT
NM_001032296.3      ------------------------------------ATGGCTCACTCCCCGGTGCAGTCG
NM_001286649.1      ------------------------------------ATGGCTCACTCCCCGGTGCAGTCG
                                                        *: ..:.. :   * .  *:. * 

HsCD00000386        GGCCTGCCCGGCATGCAGAACCTAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAGAG
NM_003576.4         CATCCTGGAGGGAGCACGAACCTAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAGAG
NM_001032296.3      GGCCTGCCCGGCATGCAGAACCTAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAGAG
NM_001286649.1      GGCCTGCCCGGCATGCAGAACCTAAAGGCAGACCCAGAAGAGCTTTTTACAAAACTAGAG
                     . *    .** *  ..*******************************************

HsCD00000386        AAAATTGGGAAGGGCTCCTTTGGAGAGGTGTTCAAAGGCATTGACAATCGGACTCAGAAA
NM_003576.4         AAAATTGGGAAGGGCTCCTTTGGAGAGGTGTTCAAAGGCATTGACAATCGGACTCAGAAA
NM_001032296.3      AAAATTGGGAAGGGCTCCTTTGGAGAGGTGTTCAAAGGCATTGACAATCGGACTCAGAAA
NM_001286649.1      AAAATTGGGAAGGGCTCCTTTGGAGAGGTGTTCAAAGGCATTGACAATCGGACTCAGAAA
                    ************************************************************

HsCD00000386        GTGGTTGCCATAAAGATCATTGATCTGGAAGAAGCTGAAGATGAGATAGAGGACATTCAA
NM_003576.4         GTGGTTGCCATAAAGATCATTGATCTGGAAGAAGCTGAAGATGAGATAGAGGACATTCAA
NM_001032296.3      GTGGTTGCCATAAAGATCATTGATCTGGAAGAAGCTGAAGATGAGATAGAGGACATTCAA
NM_001286649.1      GTGGTTGCCATAAAGATCATTGATCTGGAAGAAGCTGAAGATGAGATAGAGGACATTCAA
                    ************************************************************

HsCD00000386        CAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCATATGTAACCAAATATTATGGATCC
NM_003576.4         CAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCATATGTAACCAAATATTATGGATCC
NM_001032296.3      CAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCATATGTAACCAAATATTATGGATCC
NM_001286649.1      CAAGAAATCACAGTGCTGAGTCAGTGTGACAGTCCATATGTAACCAAATATTATGGATCC
                    ************************************************************

HsCD00000386        TATCTGAAGGATACAAAATTATGGATAATAATGGAATATCTTGGTGGAGGCTCCGCACTA
NM_003576.4         TATCTGAAGGATACAAAATTATGGATAATAATGGAATATCTTGGTGGAGGCTCCGCACTA
NM_001032296.3      TATCTGAAGGATACAAAATTATGGATAATAATGGAATATCTTGGTGGAGGCTCCGCACTA
NM_001286649.1      TATCTGAAG---------------------------------------------------
                    *********                                                   

HsCD00000386        GATCTATTAGAACCTGGCCCATTAGATGAAACCCAGATCGCTACTATATTAAGAGAAATA
NM_003576.4         GATCTATTAGAACCTGGCCCATTAGATGAAACCCAGATCGCTACTATATTAAGAGAAATA
NM_001032296.3      GATCTATTAGAACCTGGCCCATTAGATGAAACCCAGATCGCTACTATATTAAGAGAAATA
NM_001286649.1      ------TTAGAACCTGGCCCATTAGATGAAACCCAGATCGCTACTATATTAAGAGAAATA
                          ******************************************************

HsCD00000386        CTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAAATCCACAGAGACATTAAAGCGGCC
NM_003576.4         CTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAAATCCACAGAGACATTAAAGCGGCC
NM_001032296.3      CTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAAATCCACAGAGACATTAAAGCGGCC
NM_001286649.1      CTGAAAGGACTCGATTATCTCCATTCGGAGAAGAAAATCCACAGAGACATTAAAGCGGCC
                    ************************************************************

HsCD00000386        AACGTCCTGCTGTCTGAGCATGGCGAGGTGAAGCTGGCGGACTTTGGCGTGGCTGGCCAG
NM_003576.4         AACGTCCTGCTGTCTGAGCATGGCGAGGTGAAGCTGGCGGACTTTGGCGTGGCTGGCCAG
NM_001032296.3      AACGTCCTGCTGTCTGAGCATGGCGAGGTGAAGCTGGCGGACTTTGGCGTGGCTGGCCAG
NM_001286649.1      AACGTCCTGCTGTCTGAGCATGGCGAGGTGAAGCTGGCGGACTTTGGCGTGGCTGGCCAG
                    ************************************************************

HsCD00000386        CTGACAGACACCCAGATCAAAAGGAACACCTTCGTGGGCACCCCATTCTGGATGGCACCC
NM_003576.4         CTGACAGACACCCAGATCAAAAGGAACACCTTCGTGGGCACCCCATTCTGGATGGCACCC
NM_001032296.3      CTGACAGACACCCAGATCAAAAGGAACACCTTCGTGGGCACCCCATTCTGGATGGCACCC
NM_001286649.1      CTGACAGACACCCAGATCAAAAGGAACACCTTCGTGGGCACCCCATTCTGGATGGCACCC
                    ************************************************************

HsCD00000386        GAGGTCATCAAACAGTCGGCCTATGACTCGAAGGCAGACATCTGGTCCCTGGGCATAACA
NM_003576.4         GAGGTCATCAAACAGTCGGCCTATGACTCGAAGGCAGACATCTGGTCCCTGGGCATAACA
NM_001032296.3      GAGGTCATCAAACAGTCGGCCTATGACTCGAAGGCAGACATCTGGTCCCTGGGCATAACA
NM_001286649.1      GAGGTCATCAAACAGTCGGCCTATGACTCGAAGGCAGACATCTGGTCCCTGGGCATAACA
                    ************************************************************

HsCD00000386        GCTATTGAACTTGCAAGAGGGGAACCACCTCATTCCGAGCTGCACCCCATGAAAGTTTTA
NM_003576.4         GCTATTGAACTTGCAAGAGGGGAACCACCTCATTCCGAGCTGCACCCCATGAAAGTTTTA
NM_001032296.3      GCTATTGAACTTGCAAGAGGGGAACCACCTCATTCCGAGCTGCACCCCATGAAAGTTTTA
NM_001286649.1      GCTATTGAACTTGCAAGAGGGGAACCACCTCATTCCGAGCTGCACCCCATGAAAGTTTTA
                    ************************************************************

HsCD00000386        TTCCTCATTCCAAAGAACAACCCACCGACGTTGGAAGGAAACTACAGTAAACCCCTCAAG
NM_003576.4         TTCCTCATTCCAAAGAACAACCCACCGACGTTGGAAGGAAACTACAGTAAACCCCTCAAG
NM_001032296.3      TTCCTCATTCCAAAGAACAACCCACCGACGTTGGAAGGAAACTACAGTAAACCCCTCAAG
NM_001286649.1      TTCCTCATTCCAAAGAACAACCCACCGACGTTGGAAGGAAACTACAGTAAACCCCTCAAG
                    ************************************************************

HsCD00000386        GAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGCTTTAGACCCACTGCTAAGGAGTTA
NM_003576.4         GAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGCTTTAGACCCACTGCTAAGGAGTTA
NM_001032296.3      GAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGCTTTAGACCCACTGCTAAGGAGTTA
NM_001286649.1      GAGTTTGTGGAGGCCTGTTTGAATAAGGAGCCGAGCTTTAGACCCACTGCTAAGGAGTTA
                    ************************************************************

HsCD00000386        TTGAAGCACAAGTTTATACTACGCAATGCAAAGAAAACTTCCTACTTGACCGAGCTCATC
NM_003576.4         TTGAAGCACAAGTTTATACTACGCAATGCAAAGAAAACTTCCTACTTGACCGAGCTCATC
NM_001032296.3      TTGAAGCACAAGTTTATACTACGCAATGCAAAGAAAACTTCCTACTTGACCGAGCTCATC
NM_001286649.1      TTGAAGCACAAGTTTATACTACGCAATGCAAAGAAAACTTCCTACTTGACCGAGCTCATC
                    ************************************************************

HsCD00000386        GACAGGTACAAGAGATGGAAGGCCGAGCAGAGCCATGACGACTCGAGCTCCGAGGATTCC
NM_003576.4         GACAGGTACAAGAGATGGAAGGCCGAGCAGAGCCATGACGACTCGAGCTCCGAGGATTCC
NM_001032296.3      GACAGGTACAAGAGATGGAAGGCCGAGCAGAGCCATGACGACTCGAGCTCCGAGGATTCC
NM_001286649.1      GACAGGTACAAGAGATGGAAGGCCGAGCAGAGCCATGACGACTCGAGCTCCGAGGATTCC
                    ************************************************************

HsCD00000386        GACGCGGAAACAGATGGCCAAGCCTCGGGGGGCAGTGATTCTGGGGACTGGATCTTCACA
NM_003576.4         GACGCGGAAACAGATGGCCAAGCCTCGGGGGGCAGTGATTCTGGGGACTGGATCTTCACA
NM_001032296.3      GACGCGGAAACAGATGGCCAAGCCTCGGGGGGCAGTGATTCTGGGGACTGGATCTTCACA
NM_001286649.1      GACGCGGAAACAGATGGCCAAGCCTCGGGGGGCAGTGATTCTGGGGACTGGATCTTCACA
                    ************************************************************

HsCD00000386        ATCCGAGAAAAAGATCCCAAGAATCTCGAGAATGGAGCTCTTCAGCCATCGGACTTGGAC
NM_003576.4         ATCCGAGAAAAAGATCCCAAGAATCTCGAGAATGGAGCTCTTCAGCCATCGGACTTGGAC
NM_001032296.3      ATCCGAGAAAAAGATCCCAAGAATCTCGAGAATGGAGCTCTTCAGCCATCGGACTTGGAC
NM_001286649.1      ATCCGAGAAAAAGATCCCAAGAATCTCGAGAATGGAGCTCTTCAGCCATCGGACTTGGAC
                    ************************************************************

HsCD00000386        AGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTCTCTCAGTGTTTATCTACAATTATT
NM_003576.4         AGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTCTCTCAGTGTTTATCTACAATTATT
NM_001032296.3      AGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTCTCTCAGTGTTTATCTACAATTATT
NM_001286649.1      AGAAATAAGATGAAAGACATCCCAAAGAGGCCTTTCTCTCAGTGTTTATCTACAATTATT
                    ************************************************************

HsCD00000386        TCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAGGCGTGCGGAGGGAACTTGGGGTCC
NM_003576.4         TCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAGGCGTGCGGAGGGAACTTGGGGTCC
NM_001032296.3      TCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAGGCGTGCGGAGGGAACTTGGGGTCC
NM_001286649.1      TCTCCTCTGTTTGCAGAGTTGAAGGAGAAGAGCCAGGCGTGCGGAGGGAACTTGGGGTCC
                    ************************************************************

HsCD00000386        ATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAGGAGGCGTGCCCTGGCATCTCCGAC
NM_003576.4         ATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAGGAGGCGTGCCCTGGCATCTCCGAC
NM_001032296.3      ATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAGGAGGCGTGCCCTGGCATCTCCGAC
NM_001286649.1      ATTGAAGAGCTGCGAGGGGCCATCTACCTAGCGGAGGAGGCGTGCCCTGGCATCTCCGAC
                    ************************************************************

HsCD00000386        ACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGATACTCTCTAAGTGGTGGAGGAACT
NM_003576.4         ACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGATACTCTCTAAGTGGTGGAGGAACT
NM_001032296.3      ACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGATACTCTCTAAGTGGTGGAGGAACT
NM_001286649.1      ACCATGGTGGCCCAGCTCGTGCAGCGGCTCCAGAGATACTCTCTAAGTGGTGGAGGAACT
                    ************************************************************

HsCD00000386        TCATCCCACTAG
NM_003576.4         TCATCCCACTGA
NM_001032296.3      TCATCCCACTGA
NM_001286649.1      TCATCCCACTGA
                    **********..

Pathways

Reactome : Apoptosis
Reactome : Apoptotic cleavage of cellular proteins
Reactome : Apoptotic execution phase

Clone

Cloning Information : 21500
HIP Master Clone ID : 40060
Original Clone ID : FLH021500.01X

Organism


TAX_ID : 9606
Species Specific ID: 8428

Genome

Chromosome : 13
Map Location : 13q31.2-q32.3
Ensembl : ENSG00000102572

Reagents

Labome : STK24-antibody