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GSK3A (Homo sapiens) in pJP1520 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  GSK3A
Gene Name:  glycogen synthase kinase 3 alpha
Sequence             
Original Clone ID: FLH205947.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1520               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors:
HsCD00038681

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1248nts         Open reading frame : 1 to 1248
Reference Sequence Alignment

ATGAGCGGCGGCGGCGGAGGAGGCAGCGGAGGCCCCGGCGCAGGCACTAGCTTCCCGCCG
CCCGGGGTGAAGCTGGGCCGTGACAGCGGGAAGGTGACCACAGTCGTAGCCACTCTAGGC
CAAGGCCCAGAGCGCTCCCAAGAAGTGGCTTACACGGACATCAAAGTGATTGGCAATGGC
TCATTTGGGGTCGTGTACCAGGCACGGCTGGCAGAGACCAGGGAACTAGTCGCCATCAAG
AAGGTTCTCCAGGACAAGAGGTTCAAGAACCGAGAGCTGCAGATCATGCGTAAGCTGGAC
CACTGCAATATTGTGAGGCTGAGATACTTTTTCTACTCCAGTGGCGAGAAGAAAGACGAG
CTTTACCTAAATCTGGTGCTGGAATATGTGCCCGAGACAGTGTACCGGGTGGCCCGCCAC
TTCACCAAGGCCAAGTTGACCATCCCTATCCTCTATGTCAAGGTGTACATGTACCAGCTC
TTCCGCAGCTTGGCCTACATCCACTCCCAGGGCGTGTGTCACCGCGACATCAAGCCCCAG
AACCTGCTGGTGGACCCTGACACTGCTGTCCTCAAGCTCTGCGATTTTGGCAGTGCAAAG
CAGTTGGTCCGAGGGGAGCCCAATGTCTCCTACATCTGTTCTCGCTACTACCGGGCCCCA
GAGCTCATCTTTGGAGCCACTGATTACACCTCATCCATCGATGTTTGGTCAGCTGGCTGT
GTACTGGCAGAGCTCCTCTTGGGCCAGCCCATCTTCCCTGGGGACAGTGGGGTGGACCAG
CTGGTGGAGATCATCAAGGTGCTGGGAACACCAACCCGGGAACAAATCCGAGAGATGAAC
CCCAACTACACGGAGTTCAAGTTCCCTCAGATTAAAGCTCACCCCTGGACAAAGGTGTTC
AAATCTCGAACGCCGCCAGAGGCCATCGCGCTCTGCTCTAGCCTGCTGGAGTACACCCCA
TCCTCAAGGCTCTCCCCACTAGAGGCCTGTGCGCACAGCTTCTTTGATGAACTGCGATGT
CTGGGAACCCAGCTGCCTAACAACCGCCCACTTCCCCCTCTCTTCAACTTCAGTGCTGGT
GAACTCTCCATCCAACCGTCTCTCAACGCCATTCTCATCCCTCCTCACTTGAGGTCCCCA
GCGGGCACTACCACCCTCACCCCGTCCTCACAAGCTTTAACTGAGACTCCGACCAGCTCA
GACTGGCAGTCGACCGATGCCACACCTACCCTCACTAACTCCTCCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 1560
Start on reference sequence 109
Mutations:
None
Discrepancy:
del@11,204;
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  puromycin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1520

pJP1520 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7894
Parent Vector: None
Empty Vector: None
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 5659 6341
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer JPO113 forward sequencing primer: GCGGTTTTGGCAGTACATCAATGGGCG 3555 3581
primer site PBSQ primer binding sequence (glutamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3879 4005
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GSK3A
Symbol Nomenclature : GSK3A
Designation : GSK-3 alpha|glycogen synthase kinase-3 alpha|serine/threonine-protein kinase GSK3A
Full Nomenclature : glycogen synthase kinase 3 alpha
GENEID : 2931
HGNC : 4616
MIM : 606784
Vega : OTTHUMG00000150722
Target GenBank: BC051865

 

Reference Sequence Alignment

  

HsCD00038681      ------------------------------------------------------------
NM_019884.2       ATGAGCGGCGGCGGGCCTTCGGGAGGCGGCCCTGGGGGCTCGGGCAGGGCGCGGACTAGC
                                                                              

HsCD00038681      ------------------------------------------------------------
NM_019884.2       TCGTTCGCGGAGCCCGGCGGCGGAGGCGGAGGAGGCGGCGGCGGCCCCGGAGGCTCGGCC
                                                                              

HsCD00038681      ------------------------------------------------------------
NM_019884.2       TCCGGCCCAGGCGGCACCGGCGGCGGAAAGGCATCTGTCGGGGCCATGGGTGGGGGCGTC
                                                                              

HsCD00038681      ------------------------ATGAGCGGCGGCGGCGGAGGAGGCAGCGGAGGCCCC
NM_019884.2       GGGGCCTCGAGCTCCGGGGGTGGACCCGGCGGCAGCGGCGGAGGAGGCAGCGGAGGCCCC
                                          .  .*****.**************************

HsCD00038681      GGCGCAGGCACTAGCTTCCCGCCGCCCGGGGTGAAGCTGGGCCGTGACAGCGGGAAGGTG
NM_019884.2       GGCGCAGGCACTAGCTTCCCGCCGCCCGGGGTGAAGCTGGGCCGTGACAGCGGGAAGGTG
                  ************************************************************

HsCD00038681      ACCACAGTCGTAGCCACTCTAGGCCAAGGCCCAGAGCGCTCCCAAGAAGTGGCTTACACG
NM_019884.2       ACCACAGTCGTAGCCACTCTAGGCCAAGGCCCAGAGCGCTCCCAAGAAGTGGCTTACACG
                  ************************************************************

HsCD00038681      GACATCAAAGTGATTGGCAATGGCTCATTTGGGGTCGTGTACCAGGCACGGCTGGCAGAG
NM_019884.2       GACATCAAAGTGATTGGCAATGGCTCATTTGGGGTCGTGTACCAGGCACGGCTGGCAGAG
                  ************************************************************

HsCD00038681      ACCAGGGAACTAGTCGCCATCAAGAAGGTTCTCCAGGACAAGAGGTTCAAGAACCGAGAG
NM_019884.2       ACCAGGGAACTAGTCGCCATCAAGAAGGTTCTCCAGGACAAGAGGTTCAAGAACCGAGAG
                  ************************************************************

HsCD00038681      CTGCAGATCATGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGAGATACTTTTTCTAC
NM_019884.2       CTGCAGATCATGCGTAAGCTGGACCACTGCAATATTGTGAGGCTGAGATACTTTTTCTAC
                  ************************************************************

HsCD00038681      TCCAGTGGCGAGAAGAAAGACGAGCTTTACCTAAATCTGGTGCTGGAATATGTGCCCGAG
NM_019884.2       TCCAGTGGCGAGAAGAAAGACGAGCTTTACCTAAATCTGGTGCTGGAATATGTGCCCGAG
                  ************************************************************

HsCD00038681      ACAGTGTACCGGGTGGCCCGCCACTTCACCAAGGCCAAGTTGACCATCCCTATCCTCTAT
NM_019884.2       ACAGTGTACCGGGTGGCCCGCCACTTCACCAAGGCCAAGTTGACCATCCCTATCCTCTAT
                  ************************************************************

HsCD00038681      GTCAAGGTGTACATGTACCAGCTCTTCCGCAGCTTGGCCTACATCCACTCCCAGGGCGTG
NM_019884.2       GTCAAGGTGTACATGTACCAGCTCTTCCGCAGCTTGGCCTACATCCACTCCCAGGGCGTG
                  ************************************************************

HsCD00038681      TGTCACCGCGACATCAAGCCCCAGAACCTGCTGGTGGACCCTGACACTGCTGTCCTCAAG
NM_019884.2       TGTCACCGCGACATCAAGCCCCAGAACCTGCTGGTGGACCCTGACACTGCTGTCCTCAAG
                  ************************************************************

HsCD00038681      CTCTGCGATTTTGGCAGTGCAAAGCAGTTGGTCCGAGGGGAGCCCAATGTCTCCTACATC
NM_019884.2       CTCTGCGATTTTGGCAGTGCAAAGCAGTTGGTCCGAGGGGAGCCCAATGTCTCCTACATC
                  ************************************************************

HsCD00038681      TGTTCTCGCTACTACCGGGCCCCAGAGCTCATCTTTGGAGCCACTGATTACACCTCATCC
NM_019884.2       TGTTCTCGCTACTACCGGGCCCCAGAGCTCATCTTTGGAGCCACTGATTACACCTCATCC
                  ************************************************************

HsCD00038681      ATCGATGTTTGGTCAGCTGGCTGTGTACTGGCAGAGCTCCTCTTGGGCCAGCCCATCTTC
NM_019884.2       ATCGATGTTTGGTCAGCTGGCTGTGTACTGGCAGAGCTCCTCTTGGGCCAGCCCATCTTC
                  ************************************************************

HsCD00038681      CCTGGGGACAGTGGGGTGGACCAGCTGGTGGAGATCATCAAGGTGCTGGGAACACCAACC
NM_019884.2       CCTGGGGACAGTGGGGTGGACCAGCTGGTGGAGATCATCAAGGTGCTGGGAACACCAACC
                  ************************************************************

HsCD00038681      CGGGAACAAATCCGAGAGATGAACCCCAACTACACGGAGTTCAAGTTCCCTCAGATTAAA
NM_019884.2       CGGGAACAAATCCGAGAGATGAACCCCAACTACACGGAGTTCAAGTTCCCTCAGATTAAA
                  ************************************************************

HsCD00038681      GCTCACCCCTGGACAAAGGTGTTCAAATCTCGAACGCCGCCAGAGGCCATCGCGCTCTGC
NM_019884.2       GCTCACCCCTGGACAAAGGTGTTCAAATCTCGAACGCCGCCAGAGGCCATCGCGCTCTGC
                  ************************************************************

HsCD00038681      TCTAGCCTGCTGGAGTACACCCCATCCTCAAGGCTCTCCCCACTAGAGGCCTGTGCGCAC
NM_019884.2       TCTAGCCTGCTGGAGTACACCCCATCCTCAAGGCTCTCCCCACTAGAGGCCTGTGCGCAC
                  ************************************************************

HsCD00038681      AGCTTCTTTGATGAACTGCGATGTCTGGGAACCCAGCTGCCTAACAACCGCCCACTTCCC
NM_019884.2       AGCTTCTTTGATGAACTGCGATGTCTGGGAACCCAGCTGCCTAACAACCGCCCACTTCCC
                  ************************************************************

HsCD00038681      CCTCTCTTCAACTTCAGTGCTGGTGAACTCTCCATCCAACCGTCTCTCAACGCCATTCTC
NM_019884.2       CCTCTCTTCAACTTCAGTGCTGGTGAACTCTCCATCCAACCGTCTCTCAACGCCATTCTC
                  ************************************************************

HsCD00038681      ATCCCTCCTCACTTGAGGTCCCCAGCGGGCACTACCACCCTCACCCCGTCCTCACAAGCT
NM_019884.2       ATCCCTCCTCACTTGAGGTCCCCAGCGGGCACTACCACCCTCACCCCGTCCTCACAAGCT
                  ************************************************************

HsCD00038681      TTAACTGAGACTCCGACCAGCTCAGACTGGCAGTCGACCGATGCCACACCTACCCTCACT
NM_019884.2       TTAACTGAGACTCCGACCAGCTCAGACTGGCAGTCGACCGATGCCACACCTACCCTCACT
                  ************************************************************

HsCD00038681      AACTCCTCCTTG
NM_019884.2       AACTCCTCCTGA
                  ********** .

Pathways

NCI : Canonical Wnt signaling pathway
NCI : Class I PI3K signaling events mediated by Akt
NCI : Degradation of beta catenin
NCI : FOXM1 transcription factor network
Panther : Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway
Panther : Insulin/IGF pathway-protein kinase B signaling cascade
Panther : PDGF signaling pathway
Panther : Ras Pathway
Reactome : AKT phosphorylates targets in the cytosol
Reactome : Adaptive Immune System
Reactome : Constitutive PI3K/AKT Signaling in Cancer
Reactome : DAP12 interactions
Reactome : DAP12 signaling
Reactome : Disease
Reactome : Downstream signal transduction
Reactome : Downstream signaling events of B Cell Receptor (BCR)
Reactome : Downstream signaling of activated FGFR
Reactome : Fc epsilon receptor (FCERI) signaling
Reactome : GAB1 signalosome
Reactome : IRE1alpha activates chaperones
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Metabolism of proteins
Reactome : NGF signalling via TRKA from the plasma membrane
Reactome : PI-3K cascade
Reactome : PI3K events in ERBB2 signaling
Reactome : PI3K events in ERBB4 signaling
Reactome : PI3K/AKT Signaling in Cancer
Reactome : PI3K/AKT activation
Reactome : PIP3 activates AKT signaling
Reactome : Role of LAT2/NTAL/LAB on calcium mobilization
Reactome : Signal Transduction
Reactome : Signaling by EGFR
Reactome : Signaling by EGFR in Cancer
Reactome : Signaling by ERBB2
Reactome : Signaling by ERBB4
Reactome : Signaling by FGFR
Reactome : Signaling by FGFR in disease
Reactome : Signaling by PDGF
Reactome : Signaling by SCF-KIT
Reactome : Signaling by the B Cell Receptor (BCR)
Reactome : Signalling by NGF
Reactome : Unfolded Protein Response (UPR)
Reactome : XBP1(S) activates chaperone genes
WikiPathway : B Cell Receptor Signaling Pathway
WikiPathway : Glycogen Metabolism
WikiPathway : IL-5 signaling pathway
WikiPathway : Insulin Signaling
WikiPathway : Integrated Breast Cancer Pathway

Clone

Cloning Information : 205947
HIP Master Clone ID : 178231
Original Clone ID : FLH205947.01L

Organism


TAX_ID : 9606
Species Specific ID: 2931

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000105723

Reagents

Labome : GSK3-antibody