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UCK1 (Homo sapiens) in pJP1563 (retroviral expression vector)

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Explanation of Terms


Gene: Gene Symbol:  UCK1
Gene Name:  uridine-cytidine kinase 1
Sequence             
Original Clone ID: FLH214084.01L
Keyword: potential short variant
Species: Homo sapiens
Type: cDNA
Vector Name: pJP1563               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
HsCD00038807

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 606nts         Open reading frame : 1 to 606
Reference Sequence Alignment

ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
CAGCGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGGAAGTCGACCGTGTGT
GAGAAGATCATGGAGTTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTC
ATCCTGAGCCAGGACAGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTG
AAAGGACAGTACAATTTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACT
CTGAAGAACATCGTGGAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACAC
TCAAGGTTACCAGAGACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATC
TTGGTGTTCTACAGCCAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACC
GACTCCGACGTCAGGCTGTCTCGAAGAGACAAAGAAGTATGCCGATGTGATCATCCCACG
AGGAGTGGACAATATGGTTGCCATCAACCTGATCGTGCAGCACATCCAGGACATTCTGAA
TGGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Insert was fully sequenced in parent vector; Verification by restriction enzyme digest
Reference Sequence Annotations:
End on reference sequence 692
Start on reference sequence 87
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition:  100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:  Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:  Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pJP1563

pJP1563 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  JPO113
Reverse:  Unknown
Description: Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning.
Comments: One in a series of pJP# vectors that includes puroR, blastR, hygR vectors made by J. Pearlberg at HIP
Size (bp): 7692
Parent Vector: None
Empty Vector: EvNO00023115
Properties: Creator, acceptor (destination), loxP, mammalian expression, mammalian transduction, retroviral vector
Author Name: Ed Harlow
Joseph Pearlberg
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
gene fragment gag/pol/env non-functional gag/pol/env for packaging efficiency 1871 2644
primer site PBSQ primer binding site (glytamine tRNA primer binding site) 1140 1156
promoter bacterial bacterial promoter (for chlR ORF in Creator-type inserts) 3677 3803
recombination site LoxP LoxP site for recombinational cloning 3651 3684
selectable marker BlastR blasticidin resistance gene (blasticidin S deaminase) 2428 2823
selectable marker AmpR ampicillin resistance gene (beta-lactamase) 6237 7094
viral LTR 5p LTR 5p viral LTR (MPSV U3, R, U5) 550 1139
viral LTR 3p LTR 3p viral LTR (MPSV U3, R, U5) 3962 4551

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UCK1
Symbol Nomenclature : UCK1
SYNONYM : URK1
Designation : UCK 1|cytidine monophosphokinase 1|uridine monophosphokinase 1
Full Nomenclature : uridine-cytidine kinase 1
GENEID : 83549
Locus Tag : RP11-334J6.5
HGNC : 14859
MIM : 609328
Vega : OTTHUMG00000020823
Target GenBank: BC015547

 

Reference Sequence Alignment

  

HsCD00038807        ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001261451.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
XM_005272224.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
XM_005272225.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001261450.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_001135954.1      ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_031432.2         ATGGCTTCGGCGGGAGGCGAAGACTGCGAGAGCCCCGCGCCGGAGGCCGACCGTCCGCAC
NM_012474.4         ------------ATGGCCGGGGACAGCGAGCAGACCCTGCAGAACCACCAGCAGCCCAAC
                                . .* **..***:*****.. .**  **.*.*  .* * *. ** .**

HsCD00038807        CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_001261451.1      CAG---CGGCCCTTCCTGATAGGGGACGAGCGGTTCCAGGCGGGAATCCCG---------
XM_005272224.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGGAAGGACGAGCGG
XM_005272225.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_001261450.1      CAG---CGGCCCTTCCTGATAGGG------------------------------------
NM_001135954.1      CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_031432.2         CAG---CGGCCCTTCCTGATAGGGGTGAGCGGCGGCACTGCCAGCGGG------------
NM_012474.4         GGCGGCGAGCCCTTCCTTATAGGCGTCAGCGGGGGAACAGCTAGCGGCAAG---------
                     .     .********* *****                                     

HsCD00038807        ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_001261451.1      ------------------CTTCTGTGTCTCCAGTCGACCGTGTGTGAGAAGATCATGGAG
XM_005272224.1      TTCCAGGCGGGAATCCCGCTTCTGTGTCTCCAGTCGACCGTGTGTGAGAAGATCATGGAG
XM_005272225.1      ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_001261450.1      ---------------------------------TCGACCGTGTGTGAGAAGATCATGGAG
NM_001135954.1      ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_031432.2         ------------------------------AAGTCGACCGTGTGTGAGAAGATCATGGAG
NM_012474.4         ---------------------------------TCTTCCGTGTGTGCTAAGATCGTGCAG
                                                     ** :*********. ******.** **

HsCD00038807        TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001261451.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
XM_005272224.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
XM_005272225.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001261450.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_001135954.1      TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_031432.2         TTGCTGGGACAGAACGAGGTGGAACAGCGGCAGCGGAAGGTGGTCATCCTGAGCCAGGAC
NM_012474.4         CTCCTGGGGCAGAATGAGGTGGACTATCGCCAGAAGCAGGTGGTCATCCTGAGCCAGGAT
                     * *****.***** ********. * ** ***..*.********************** 

HsCD00038807        AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001261451.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
XM_005272224.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
XM_005272225.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001261450.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_001135954.1      AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_031432.2         AGGTTCTACAAGGTCCTGACGGCAGAGCAGAAGGCCAAGGCCTTGAAAGGACAGTACAAT
NM_012474.4         AGCTTCTACCGTGTCCTTACCTCGGAGCAGAAGGCCAAAGCCCTGAAGGGCCAGTTCAAC
                    ** ******.. ***** **  *.**************.*** ****.**.****:*** 

HsCD00038807        TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001261451.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
XM_005272224.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
XM_005272225.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001261450.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_001135954.1      TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_031432.2         TTTGACCATCCAGATGCCTTTGATAATGATTTGATGCACAGGACTCTGAAGAACATCGTG
NM_012474.4         TTTGACCACCCGGATGCCTTTGACAATGAACTCATTCTCAAAACACTCAAAGAAATCACT
                    ******** **.*********** *****: * ** *:**..**:** **..*.***.  

HsCD00038807        GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_001261451.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
XM_005272224.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
XM_005272225.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAG----------
NM_001261450.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_001135954.1      GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_031432.2         GAGGGCAAAACGGTGGAGGTGCCGACCTATGATTTTGTGACACACTCAAGGTTACCAGAG
NM_012474.4         GAAGGGAAAACAGTCCAGATCCCCGTGTATGACTTTGTCTCCCATTCCCGGAAGGAGGAG
                    **.** *****.**  **.* ** .  ***** ***** :*.** **..*          

HsCD00038807        ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_001261451.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
XM_005272224.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
XM_005272225.1      -----------------------------------------------------------C
NM_001261450.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_001135954.1      ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_031432.2         ACCACGGTGGTCTACCCTGCGGACGTGGTTCTGTTTGAGGGCATCTTGGTGTTCTACAGC
NM_012474.4         ACAGTTACTGTCTATCCCGCAGACGTGGTGCTCTTTGAAGGGATCCTGGCCTTCTACTCC
                                                                               *

HsCD00038807        CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001261451.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
XM_005272224.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
XM_005272225.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001261450.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_001135954.1      CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_031432.2         CAGGAGATCCGGGACATGTTCCACCTGCGCCTCTTCGTGGACACCGACTCCGACGTCAGG
NM_012474.4         CAGGAGGTACGAGACCTGTTCCAGATGAAGCTTTTTGTGGATACAGATGCGGACACCCGG
                    ******.*.**.***.******* .**.. ** ** ***** **.**  * ***. *.**

HsCD00038807        CTGTCTCGAAGAG-----------------------------------------------
NM_001261451.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
XM_005272224.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
XM_005272225.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_001261450.1      CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_001135954.1      CTGTCTCGAAGAG-----------------------------------------------
NM_031432.2         CTGTCTCGAAGAGTTCTCCGGGACGTGCGC---CGAGGGAGGGACCTGGAGCAGATTCTG
NM_012474.4         CTCTCACGCAGAGTATTAAGGGACATCAGCGAGAGAGGCAGGGATCTTGAGCAGATTTTA
                    ** **:**.****                                               

HsCD00038807        ---------------------------------------------------ACAAAGAAG
NM_001261451.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
XM_005272224.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
XM_005272225.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_001261450.1      ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_001135954.1      ---------------------------------------------------ACAAAGAAG
NM_031432.2         ACGCAGTACACCACCTTCGTGAAGCCGGCCTTCGAGGAGTTCTGCCTGCCGACAAAGAAG
NM_012474.4         TCTCAGTACATTACGTTCGTCAAGCCTGCCTTTGAGGAATTCTGCTTGCCAACAAAGAAG
                                                                       *********

HsCD00038807        TATGCCGATGTGATCATCCCACGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001261451.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
XM_005272224.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
XM_005272225.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001261450.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_001135954.1      TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_031432.2         TATGCCGATGTGATCATCCCGCGAGGAGTGGACAATATGGTTGCCATCAACCTGATCGTG
NM_012474.4         TATGCTGATGTGATCATCCCTAGAGGTGCAGATAATCTGGTGGCCATCAACCTCATCGTG
                    ***** ************** .****:* .** ***.**** *********** ******

HsCD00038807        CAGCACATCCAGGACATTCTGAATGGTTG-------------------------------
NM_001261451.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
XM_005272224.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
XM_005272225.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_001261450.1      CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_001135954.1      CAGCACATCCAGGACATTCTGAATGGTGA-------------------------------
NM_031432.2         CAGCACATCCAGGACATTCTGAATGGTGACATCTGCAAATGGCACCGAGGAGGGTCCAAT
NM_012474.4         CAGCACATCCAGGACATCCTGAATGGAGGGCCCTCCAAACGGCAGACCAATGG-------
                    ***************** ********: .                               

HsCD00038807        ------------------------------------------------------------
NM_001261451.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
XM_005272224.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
XM_005272225.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_001261450.1      GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_001135954.1      ------------------------------------------------------------
NM_031432.2         GGGCGGAGCTACAAGCGGACCTTTTCTGAGCCAGGGGACCACCCTGGGATGCTGACCTCT
NM_012474.4         -----------------------------------CTGTCTCAACGGCTACACCCCTTCA
                                                                                

HsCD00038807        ------------------------------------------
NM_001261451.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
XM_005272224.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
XM_005272225.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_001261450.1      GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_001135954.1      ------------------------------------------
NM_031432.2         GGCAAACGGTCACATTTGGAGTCCAGCAGCAGACCCCACTGA
NM_012474.4         CGCAAGAGGCAGGCATCGGAGTCCAGCAGCAGGCCGCATTGA
                                                              

Pathways

Panther : Salvage pyrimidine ribonucleotides
Reactome : Metabolism
Reactome : Metabolism of nucleotides
Reactome : Pyrimidine metabolism
Reactome : Pyrimidine salvage reactions
WikiPathway : Fluoropyrimidine Activity

Protein

UniProt : Q9HA47
HPRD : 18256

Clone

Cloning Information : 214084
HIP Master Clone ID : 87343
Original Clone ID : FLH214084.01L

Organism


TAX_ID : 9606
Species Specific ID: 83549

Genome

Chromosome : 9
Map Location : 9q34.13
Ensembl : ENSG00000130717

Reagents

Labome : UCK1-antibody