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TM1545 (Thermotoga maritima) in pMH4 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  TM1545
Gene Name:  hypothetical protein
Sequence             
Original Clone ID: 13109
Keyword: full-length cds
Species: Thermotoga maritima
Type: cDNA
Vector Name: pMH4               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: High number of silent and/or missense mutations in CDS.
Mutation/
Discrepancy :
Yes / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
TmCD00396383

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 657nts         Open reading frame : 51 to 707

AGATATACATACCCATGGGATCTGATAAAATTCATCATCATCATCATCACATGGATATTC
AACTTTTTCAAGGATACGGTGGTAAAGTGATAGTCGCTGTGGATTACGGAGAAAGGAAGT
GTGGAGTCGCATTTGGAGAAATTCTTCCTCAAAAAAGTCTTGTGATACCCACAAAAAACC
TGAAGGAATTCATCAGAAAGCTGAAACCTGATAAAATAATCTTTGGATTGCCTCTTTCAA
TGAGTGGAAAATACACCCAGCAGACGTTCAAAACAGTCGCGGTTGCCTTCAAATTTTCAA
AAGAGTACGAAACATATCTCTGTGACGAAAGACTCACCACGAAAATAGGAGAAAGAATCT
CGAAGAGAGATGACGCCGTGAGCGCCGCTCTGATTTTTCAGTCTTTCTTCGAGAATTCCT
CGGTATGTGAAAAAGTCACAGATCCAAGAAAAAAAGTCGATTTGGCTTTGGAAAAAGTCG
ATGGAGAAGTTCTCCTGTACGAGTTCCCAGATCCTTCTTTGAACATCGAAGCAAGAGAAG
TTGATGTGGTCACGAAAAATCCGGTTCTTGCTTATTTCTACAGTAAAAATGGATACTTCG
TTGGAAGAGAACTTTGGGAAAAGAAGTACGACCTGATCATTTCTGGAAAAAACTGCGAAG
AGTTGAAGAAATACCTGAAAGAAAACGGTAGGCTGGTGTGCCTGTAACGCGACTTAATTA
ACTCTAGATAACTGAGAAGGAGATATACATACCCGGGT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
V1M

Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCAC

3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 359767
Recommended expression in: HK100
       DyNA Vector Map

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Vector Name: pMH4

pMH4 in advanced viewed

Synonyms: pMH-4
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHH tag; amp resistance; restriction enyzme (Pml I/Pac I) or PIPE cloning.
Comments: None
Size (bp): 5489
Parent Vector: None
Empty Vector: None
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1 bacterial origin of replication 3331 4013
inducible trxn activator protein gene araC araC cds 5463 4585
negative selection marker ccdB ccdB negative selection gene (may be gone in with-insert form) 1576 1785
promoter T7 T7 promoter 290 310
promoter araC araC promoter 153 124
selectable marker CmR chloramphenicol resistance gene (CmR) 484 1140
selectable marker AmpR ampicillin resistance gene 2574 3233
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2238 2264

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : TM1545
GENEID : 897559
Locus Tag : TM1545
GI : 15644293
GenBank Accession : AAD36612
Target GenBank: NP_229345

 

Protein

SMART domain : YqgFc : Likely ribonuclease with RNase H fold.
Structural Annotation Wiki : TOPSAN
UniProt : Q9X1N1

Clone

Cloning Information : 453452
HIP Master Clone ID : 382938
Original Clone ID : 13109

Experimental

TargetTrack - Experimental data : 359767

Organism


TAX_ID : 243274
Species Specific ID: 897559

Reagents

Labome : TM1545-cDNA