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CCIN (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CCIN
Gene Name:  calicin
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH263631.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00039668

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1767nts         Open reading frame : 1 to 1767
Reference Sequence Alignment

ATGAAATTGGAATTCACGGAGAAAAACTACAATAGCTTCGTGCTGCAGAACCTGAACAGA
CAGAGGAAACGCAAAGAGTACTGGGACATGGCCCTGAGCGTGGACAACCACGTCTTCTTT
GCACATCGCAATGTGCTGGCTGCTGTCTCCCCACTGGTGAGGAGCCTCATCTCCAGCAAT
GACATGAAGACCGCTGATGAGCTTTTCATCACCATTGACACCAGTTACCTGAGCCCGGTC
ACAGTGGACCAGCTTCTGGACTACTTCTATAGCGGCAAGGTGGTGATCTCCGAGCAAAAT
GTGGAGGAGCTGCTTCGTGGGGCTCAGTATTTCAACACACCACGCCTTCGAGTTCACTGT
AACGACTTCCTTATTAAGTCCATCTGCCGTGCCAACTGCTTGCGCTACCTCTTCTTGGCT
GAGCTGTTTGAGCTCAAAGAGGTATCAGACGTAGCTTACTCTGGCATTCGGGACAACTTC
CACTACTGGGCCAGTCCTGAGGGCTCCATGCACTTCATGCGCTGTCCACCTGTTATCTTT
GGCCGCCTGCTCCGTGATGAAAACCTTCACGTGCTCAATGAAGACCAGGCGCTCAGCGCA
CTCATCAATTGGGTGTACTTCCGGAAGGAGGATCGGGAGAAGTATTTCAAGAAGTTCTTC
AATTACATCAATCTCAATGCTGTCTCCAATAAGACGCTGGTGTTTGCCAGCAACAAGCTG
GTGGGCATGGAGAACACCTCATCCCATACAACCCTGATTGAGAGTGTCCTGATGGACCGC
AAGCAGGAGCGGCCATGCAGCCTGCTGGTCTACCAGCGGAAAGGGGCCCTGCTTGATTCC
GTGGTCATCCTCGGTGGCCAGAAGGCCCACGGCCAGTTCAATGATGGAGTGTTTGCTTAT
ATCATCCAGGAGAACCTGTGGATGGAGCTCTCAGACATGCCCTATCGGGCAGCAGCACTT
AGTGCCACCTCTGCTGGTCGCTACATCTACATCTCTGGTGGCACCACTGAGCAGATTTCA
GGGCTGAAGACAGCCTGGCGGTATGACATGGATGACAACTCCTGGACCAAGTTGCCTGAC
CTGCCCATCGGGCTTGTCTTCCACACCATGGTGACCTGTGGGGGAACAGTGTACTCAGTG
GGCGGGAGCATTGCCCCAAGGCGGTATGTCTCCAACATCTATCGCTATGATGAGCGGAAG
GAAGTCTGGTGCCTGGCAGGAAAGATGAGCATCCCCATGGATGGCACCGCCGTGATCACT
AAAGGAGACAGGCATCTGTACATTGTCACTGGACGGTGCTTGGTGAAAGGTTATATCTCC
CGGGTCGGGGTAGTGGACTGCTTTGACACCAGCACTGGGGACGTGGTCCAGTGTATCACC
TTCCCCATTGAGTTCAACCATCGGCCCCTGCTCTCTTTCCAACAGGACAACATCCTCTGC
GTGCACAGCCACCGGCAGAGTGTGGAAATCAATCTGCAGAAGGTGAAGGCAAGCAAGACG
ACCACCTCAGTGCCTGTCTTGCCCAACAGCTGCCCCTTGGATGTGTCCCATGCTATATGC
TCCATTGGAGACAGCAAGGTGTTTGTATGTGGGGGTGTCACCACTGCCAGCGATGTCCAG
ACAAAGGACTACACCATCAATCCAAATGCCTTCTTGCTGGACCAAAAGACAGGCAAGTGG
AAGACCCTGGCTCCTCCACCAGAGGCACTGGACTGTCCTGCCTGCTGTCTAGCCAAGCTA
CCTTGCAAGATTCTTCAAAGGATTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1846
Start on reference sequence 80
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCIN
Symbol Nomenclature : CCIN
SYNONYM : BTBD20; KBTBD14
Full Nomenclature : calicin
GENEID : 881
Locus Tag : RP11-421H8.4
HGNC : 1568
MIM : 603960
Vega : OTTHUMG00000019901
Target GenBank: BC019251

 

Reference Sequence Alignment

  

HsCD00039668      ATGAAATTGGAATTCACGGAGAAAAACTACAATAGCTTCGTGCTGCAGAACCTGAACAGA
NM_005893.2       ATGAAATTGGAATTCACGGAGAAAAACTACAATAGCTTCGTGCTGCAGAACCTGAACAGA
                  ************************************************************

HsCD00039668      CAGAGGAAACGCAAAGAGTACTGGGACATGGCCCTGAGCGTGGACAACCACGTCTTCTTT
NM_005893.2       CAGAGGAAACGCAAAGAGTACTGGGACATGGCCCTGAGTGTGGACAACCACGTCTTCTTT
                  ************************************** *********************

HsCD00039668      GCACATCGCAATGTGCTGGCTGCTGTCTCCCCACTGGTGAGGAGCCTCATCTCCAGCAAT
NM_005893.2       GCACATCGCAATGTGCTGGCTGCTGTCTCCCCACTGGTGAGGAGCCTCATCTCCAGCAAT
                  ************************************************************

HsCD00039668      GACATGAAGACCGCTGATGAGCTTTTCATCACCATTGACACCAGTTACCTGAGCCCGGTC
NM_005893.2       GACATGAAGACCGCTGATGAGCTTTTCATCACCATTGACACCAGTTACCTGAGCCCGGTC
                  ************************************************************

HsCD00039668      ACAGTGGACCAGCTTCTGGACTACTTCTATAGCGGCAAGGTGGTGATCTCCGAGCAAAAT
NM_005893.2       ACAGTGGACCAGCTTCTGGACTACTTCTATAGCGGCAAGGTGGTGATCTCCGAGCAAAAT
                  ************************************************************

HsCD00039668      GTGGAGGAGCTGCTTCGTGGGGCTCAGTATTTCAACACACCACGCCTTCGAGTTCACTGT
NM_005893.2       GTGGAGGAGCTGCTTCGTGGGGCTCAGTATTTCAACACACCACGCCTTCGAGTTCACTGT
                  ************************************************************

HsCD00039668      AACGACTTCCTTATTAAGTCCATCTGCCGTGCCAACTGCTTGCGCTACCTCTTCTTGGCT
NM_005893.2       AACGACTTCCTTATTAAGTCCATCTGCCGTGCCAACTGCTTGCGCTACCTCTTCTTGGCT
                  ************************************************************

HsCD00039668      GAGCTGTTTGAGCTCAAAGAGGTATCAGACGTAGCTTACTCTGGCATTCGGGACAACTTC
NM_005893.2       GAGCTGTTTGAGCTCAAAGAGGTATCAGACGTAGCTTACTCTGGCATTCGGGACAACTTC
                  ************************************************************

HsCD00039668      CACTACTGGGCCAGTCCTGAGGGCTCCATGCACTTCATGCGCTGTCCACCTGTTATCTTT
NM_005893.2       CACTACTGGGCCAGTCCTGAGGGCTCCATGCACTTCATGCGCTGTCCACCTGTTATCTTT
                  ************************************************************

HsCD00039668      GGCCGCCTGCTCCGTGATGAAAACCTTCACGTGCTCAATGAAGACCAGGCGCTCAGCGCA
NM_005893.2       GGCCGCCTGCTCCGTGATGAAAACCTTCACGTGCTCAATGAAGACCAGGCGCTCAGCGCA
                  ************************************************************

HsCD00039668      CTCATCAATTGGGTGTACTTCCGGAAGGAGGATCGGGAGAAGTATTTCAAGAAGTTCTTC
NM_005893.2       CTCATCAATTGGGTGTACTTCCGGAAGGAGGATCGGGAGAAGTATTTCAAGAAGTTCTTC
                  ************************************************************

HsCD00039668      AATTACATCAATCTCAATGCTGTCTCCAATAAGACGCTGGTGTTTGCCAGCAACAAGCTG
NM_005893.2       AATTACATCAATCTCAATGCTGTCTCCAATAAGACGCTGGTGTTTGCCAGCAACAAGCTG
                  ************************************************************

HsCD00039668      GTGGGCATGGAGAACACCTCATCCCATACAACCCTGATTGAGAGTGTCCTGATGGACCGC
NM_005893.2       GTGGGCATGGAGAACACCTCATCCCATACAACCCTGATTGAGAGTGTCCTGATGGACCGC
                  ************************************************************

HsCD00039668      AAGCAGGAGCGGCCATGCAGCCTGCTGGTCTACCAGCGGAAAGGGGCCCTGCTTGATTCC
NM_005893.2       AAGCAGGAGCGGCCATGCAGCCTGCTGGTCTACCAGCGGAAAGGGGCCCTGCTTGATTCC
                  ************************************************************

HsCD00039668      GTGGTCATCCTCGGTGGCCAGAAGGCCCACGGCCAGTTCAATGATGGAGTGTTTGCTTAT
NM_005893.2       GTGGTCATCCTCGGTGGCCAGAAGGCCCACGGCCAGTTCAATGATGGAGTGTTTGCTTAT
                  ************************************************************

HsCD00039668      ATCATCCAGGAGAACCTGTGGATGGAGCTCTCAGACATGCCCTATCGGGCAGCAGCACTT
NM_005893.2       ATCATCCAGGAGAACCTGTGGATGAAGCTCTCAGACATGCCCTATCGGGCAGCAGCACTT
                  ************************.***********************************

HsCD00039668      AGTGCCACCTCTGCTGGTCGCTACATCTACATCTCTGGTGGCACCACTGAGCAGATTTCA
NM_005893.2       AGTGCCACCTCTGCTGGTCGCTACATCTACATCTCTGGTGGCACCACTGAGCAGATTTCA
                  ************************************************************

HsCD00039668      GGGCTGAAGACAGCCTGGCGGTATGACATGGATGACAACTCCTGGACCAAGTTGCCTGAC
NM_005893.2       GGGCTGAAGACAGCCTGGCGGTATGACATGGATGACAACTCCTGGACCAAGTTGCCTGAC
                  ************************************************************

HsCD00039668      CTGCCCATCGGGCTTGTCTTCCACACCATGGTGACCTGTGGGGGAACAGTGTACTCAGTG
NM_005893.2       CTGCCCATCGGGCTTGTCTTCCACACCATGGTGACCTGTGGGGGGACAGTGTACTCAGTG
                  ********************************************.***************

HsCD00039668      GGCGGGAGCATTGCCCCAAGGCGGTATGTCTCCAACATCTATCGCTATGATGAGCGGAAG
NM_005893.2       GGCGGGAGCATTGCCCCAAGGCGGTATGTCTCCAACATCTATCGCTATGATGAGCGGAAG
                  ************************************************************

HsCD00039668      GAAGTCTGGTGCCTGGCAGGAAAGATGAGCATCCCCATGGATGGCACCGCCGTGATCACT
NM_005893.2       GAAGTCTGGTGCCTGGCAGGAAAGATGAGCATCCCCATGGATGGCACCGCCGTGATCACT
                  ************************************************************

HsCD00039668      AAAGGAGACAGGCATCTGTACATTGTCACTGGACGGTGCTTGGTGAAAGGTTATATCTCC
NM_005893.2       AAAGGAGACAGGCATCTGTACATTGTCACTGGACGGTGCTTGGTGAAAGGTTATATCTCC
                  ************************************************************

HsCD00039668      CGGGTCGGGGTAGTGGACTGCTTTGACACCAGCACTGGGGACGTGGTCCAGTGTATCACC
NM_005893.2       CGGGTCGGGGTAGTGGACTGCTTTGACACCAGCACTGGGGACGTGGTCCAGTGTATCACC
                  ************************************************************

HsCD00039668      TTCCCCATTGAGTTCAACCATCGGCCCCTGCTCTCTTTCCAACAGGACAACATCCTCTGC
NM_005893.2       TTCCCCATTGAGTTCAACCATCGGCCCCTGCTCTCTTTCCAACAGGACAACATCCTCTGC
                  ************************************************************

HsCD00039668      GTGCACAGCCACCGGCAGAGTGTGGAAATCAATCTGCAGAAGGTGAAGGCAAGCAAGACG
NM_005893.2       GTGCACAGCCACCGGCAGAGTGTGGAAATCAATCTGCAGAAGGTGAAGGCAAGCAAGACG
                  ************************************************************

HsCD00039668      ACCACCTCAGTGCCTGTCTTGCCCAACAGCTGCCCCTTGGATGTGTCCCATGCTATATGC
NM_005893.2       ACCACCTCAGTGCCTGTCTTGCCCAACAGCTGCCCCTTGGATGTGTCCCATGCTATATGC
                  ************************************************************

HsCD00039668      TCCATTGGAGACAGCAAGGTGTTTGTATGTGGGGGTGTCACCACTGCCAGCGATGTCCAG
NM_005893.2       TCCATTGGAGACAGCAAGGTGTTTGTATGTGGGGGTGTCACCACTGCCAGCGATGTCCAG
                  ************************************************************

HsCD00039668      ACAAAGGACTACACCATCAATCCAAATGCCTTCTTGCTGGACCAAAAGACAGGCAAGTGG
NM_005893.2       ACAAAGGACTACACCATCAATCCAAATGCCTTCTTGCTGGACCAAAAGACAGGCAAGTGG
                  ************************************************************

HsCD00039668      AAGACCCTGGCTCCTCCACCAGAGGCACTGGACTGTCCTGCCTGCTGTCTAGCCAAGCTA
NM_005893.2       AAGACCCTGGCTCCTCCACCAGAGGCACTGGACTGTCCTGCCTGCTGTCTAGCCAAGCTA
                  ************************************************************

HsCD00039668      CCTTGCAAGATTCTTCAAAGGATTTTG
NM_005893.2       CCTTGCAAGATTCTTCAAAGGATTTAA
                  *************************:.

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : Kelch :
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
UniProt : Q13939
UniProt : Q8WX35
UniProt : Q8WWB2
HPRD : 04907

Clone

Cloning Information : 263631
HIP Master Clone ID : 196419
Original Clone ID : FLH263631.01L

Organism


TAX_ID : 9606
Species Specific ID: 881

Genome

Chromosome : 9
Map Location : 9p13.3
Ensembl : ENSG00000185972

Reagents

Labome : calicin-antibody