| Gene: |
Gene Symbol:
Gene Name: None Sequence |
|---|---|
| Original Clone ID: | AtR139-21.1 |
| Keyword: | None |
| Species: | Agrobacterium tumefaciens |
| Type: | cDNA |
| Vector Name: | pET21_NESG Format: FUSION |
| Source: | Northeast Structural Genomics Consortium |
| Description: | None |
| Comments: | None |
| Mutation/ Discrepancy : |
No / Yes |
| Publications: | None |
| Authors: |
PSI Northeast Structural Genomics Consortium |
|
AtCD00397356 Price: Login for Pricing AVAILABLE No restriction Special MTA: PSI/NESG |
Insert sequence: 1554nts
Open reading frame : 50 to 1603GGGGAGTATTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGAGTGTCAC
CGTTTTTATTCCCGGCGATTCCGCCGCACTTGCCGTTGGCGCAAACCGTGTTGCCGAAGC
GATCGCTCGAGAGGCGGCCAGCCGCAACCTCGACGTCCAGATCGTCCGTAACGGTTCGCG
CGGCATGCTCTGGCTCGAGGTGCTGGTGGAAGTGCGCACCGAGCATGGCCGTATCGCCTA
CGGGCCGGTAAAGGCCGCCGATGTCACGTCACTTTTCGATGCGGATTTTCTTGACGGTGG
CGATCACCGTCTCTGTCTTGGGCCGACGAAGGACATCCCCTTCCTGAAAAACCAGACGCG
CCTGACATTTGCCCGGTGCGGCGTTACCGACCCGCTGTCGCTAGAGGATTACCGCAACTA
TCAGGGCATGAAAGGCCTCGAAAAAGCCGTCACCATGGAGCCGCTCGCCATCGTTGCGCA
GGTGACTGAAAGCGGCCTGCGTGGACGCGGCGGCGCGGGTTTCCCGACCGGCATCAAATG
GAAGACCGTCGCAGATGCGAGGGCAGATCAGAAATATATCGTCTGCGATGCCGATGAAGG
CGATAGCGGCACCTTCGCCGACCGCATGATCATGGAAGGCGACCCCTTCGTGCTGATCGA
GGGCATGGCGATTGCCGGCCTTGCCGTTGGTGCCACCAAGGGCTTCATCTACACCCGGTC
GGAATATCCATACGCCATCAAGGTCATGGAAAAGGCGATCGAGATCGCGAGGCGTGAGGG
CATTCTTGGGTCCTCCGTCCTCGGTTCAGGCCGCGCTTTCGATATGGAAGTACGCATGGG
CGCCGGCGCCTATGTCTGCGGCGAGGAGACGTCGCTCCTGAACAGCCTCGAAGGCAAACG
CGGCACGGTGCGCGCCAAACCGCCGCTGCCGGCATTGCAGGGCCTGTTCGGCAAGCCGAC
CGTCGTCAACAACGCGATTTCGCTGGCGTCCATCCCGGTCATCATGGATCGCGGTGCCGC
TTTCTATCGCGATTTCGGCGTCGGCCGGTCGCATGGCACCATCCCCATCCAGCTCGCCGG
AAACATCAGATATGGCGGGCTTTACGAAACCGCCTTCGGTCTGACGCTCGGGCAACTGGT
GAACGATATCGGCGGCGGCACGATCACCGGCCGCCCCGTTAAGGCGGTGCAGGTGGGCGG
CCCGCTTGGCGCCTATTTTCCGGTGTCGTTGTTCGATACGATCTTCGACTACGAAGCGTT
CACGGCGGCTGGCGGTCTTATCGGACACGCCGGGATCGTGGTTTTCGACGACACCGCCGA
TATGCTGCATCAGGCTCGGTTCGCCCTTGAATTCTGCGCGGTTGAAAGCTGCGGCAAGTG
CACGCCCTGTCGTATCGGCTCGACGCGCGGCGTCGAGACTGTGGACAAGATCGCGCTTGG
CATCGAGCGGGAGAAAAACACGGCGCTGCTCGAAGATCTCTGCGAAACGATGAAATTTGG
GTCGCTTTGCGCGCTGGGCGGTTTCACGCCCTATCCCGTTATGAGCGCGCTCAGACATTT
CCCGCAAGATTTTGCCCCCATCCCACGTGTTGAAGCGGCGGAG
|
| Coding Sequence Details | |
|---|---|
| Insert Sequence Verified?: Y | Verification Method: Sequence Verification |
|
Mutations: None |
Discrepancy:a538g,N180D;t926c,V309A;
c1314t,V438V;c1374g,V458V
|
5' Linker Sequence:
AAGGAGATATACAT |
|
3' Linker Sequence:
GCGGCCGCACTCGAGCACCACCACCACCACCACTGA |
|
| Distributed in bacterial strain : | DH5-alpha T1 phage resistant |
|---|---|
| Antibiotic Selection: |
Host Type:
bacterial
Marker:
ampicillin Bacterial Selection Condition: 100 ug/mL ampicillin Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended. Comments: Commonly used conditions for ampicillin resistant plasmid clones. |
| Protein Expression Results: |
ProteinExpressed: Protein_Confirmed ProteinPurified: Protein_Purified SolubleProtein: Protein_Soluble Find experimental protocols in AtR139 |
| Recommended expression in: | E. coli BL21 |
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||
| Vector Name: | pET21_NESG |
pET21_NESG in advanced viewed |
| Synonyms: | pET21, pET21-modified, pET21Cc, pET21_NESG | |
| Sequencing Primer: | Forward:
T7
Reverse: T7 terminator |
|
| Description: | Bacterial (E. coli) expression vector with T7 lac promoter, adds C-terminal 6xHis tag; ampicillin resistance; restriction enzyme cloning. | |
| Comments: | Modified Novagen pET vector-common MCS | |
| Size (bp): | 5415 | |
| Parent Vector: | None | |
| Empty Vector: | EvNO00336944 | |
| Properties: | bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker | |
| Author Name: |
Northeast Structural Genomics Consortium Gaetano T. Montleione Thomas B. Acton |
|
| Publications: |
PMID:
15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium |
|
| Vector Map: |
Vector Sequence:
|
|
| Sequence: |
Vector Features:
| Type | Name | Description | Start Position | End Position |
|---|---|---|---|---|
| MCS | MCS | MCS (includes SphI, XbaI, BamHI, EcoRI, XhoI) | 157 | 253 |
| bacterial origin | ori | pBR322-type bacterial origin of replication | 3180 | 3862 |
| gene fragment | ROP | ROP | 2580 | 2756 |
| primer | T7 forward | T7 forward sequencing primer | 283 | 298 |
| primer | T7 reverse | T7 reverse sequencing primer | 66 | 84 |
| promoter | T7 | T7 promoter | 280 | 299 |
| repressor binding site | lacO | lacO regulatory sequence | 258 | 280 |
| repressor protein gene | LacI | LacI | 752 | 1768 |
| selectable marker | AmpR | ampicillin resistance gene | 3960 | 4619 |
| ssDNA origin | f1 ori | f1 origin | 5081 | 5387 |
| tag | His | C-terminal 6xHis tag | 140 | 157 |
| trxn termination sequence | T7 term | T7 termination sequence | 68 | 72 |
- Protein
- Clone
- Experimental
Protein
| SMART domain : | NADH_4Fe-4S : NADH-ubiquinone oxidoreductase-F iron-sulfur binding region |
|---|---|
| Structural Annotation Wiki : | TOPSAN |
| UniProt : | Q8U6U9_AGRT5 |
Clone
| Cloning Information : | 534590 |
|---|---|
| HIP Master Clone ID : | 444910 |
| Original Clone ID : | AtR139-21.1 |
Experimental
| TargetTrack - Experimental data : | AtR139 |
|---|
