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SLC6A7 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SLC6A7
Gene Name:  solute carrier family 6 (neurotransmitter transporter), member 7
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072649)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00398457

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1908nts         Open reading frame : 1 to 1908
Reference Sequence Alignment

ATGAAGAAGCTCCAGGGAGCTCACCTCCGCAAGCCTGTCACCCCAGACCTGCTGATGACC
CCCAGTGACCAGGGCGATGTCGACCTGGATGTGGACTTTGCTGCACACCGGGGGAACTGG
ACAGGCAAGCTGGACTTCCTGCTGTCCTGCATTGGCTACTGTGTAGGCCTGGGGAATGTC
TGGCGCTTCCCCTATCGAGCGTACACCAATGGAGGAGGCGCCTTCCTCGTGCCCTACTTC
CTCATGCTGGCCATCTGTGGCATCCCCCTCTTCTTCCTGGAGCTCTCCCTGGGCCAGTTC
TCCAGCCTAGGGCCCCTGGCTGTCTGGAAAATCAGCCCTCTCTTCAAAGGCGCCGGCGCA
GCCATGCTGCTCATCGTGGGCTTGGTGGCCATCTACTACAACATGATCATCGCCTACGTG
CTCTTCTACCTCTTCGCCTCCCTCACCAGCGACCTACCCTGGGAGCACTGTGGCAACTGG
TGGAACACAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGGGGCCCTGCCC
CTCAACCTCACCTGCACCGTCAGCCCCAGCGAGGAGTACTGGAGCCGCTACGTCCTCCAC
ATCCAAGGCAGCCAGGGCATCGGCAGCCCTGGGGAGATCCGCTGGAACCTCTGCCTCTGC
CTGCTGCTGGCCTGGGTCATCGTGTTCCTCTGTATCCTCAAGGGTGTGAAGTCTTCGGGC
AAGGTGGTGTATTTCACGGCCACGTTCCCCTACCTCATCCTGCTCATGCTGCTGGTCCGC
GGAGTCACCCTCCCAGGGGCCTGGAAGGGCATCCAGTTCTATCTCACCCCCCAGTTCCAC
CACTTGTTGTCTTCCAAGGTGTGGATTGAAGCTGCTCTTCAGATCTTCTATTCCCTGGGT
GTGGGCTTCGGGGGGCTCCTCACCTTTGCCTCCTACAACACGTTTCACCAGAACATCTAT
AGAGACACTTTCATCGTCACTCTGGGCAACGCCATCACCAGCATCCTGGCTGGCTTTGCC
ATCTTCTCCGTGCTGGGCTACATGTCTCAGGAGCTGGGCGTGCCTGTGGACCAAGTAGCC
AAAGCAGGCCCTGGCCTGGCCTTTGTCGTCTACCCACAGGCCATGACCATGCTGCCTCTG
TCACCCTTCTGGTCCTTTCTCTTCTTCTTCATGCTTCTGACTCTCGGCCTAGACAGCCAG
TTTGCTTTTCTGGAGACCATTGTGACAGCTGTGACAGATGAGTTCCCATACTACCTGCGG
CCCAAGAAGGCGGTGTTCTCAGGGCTCATCTGCGTGGCCATGTACCTGATGGGGCTGATC
CTCACCACTGATGGGGGCATGTACTGGCTGGTCCTTCTGGATGACTACAGCGCCAGCTTC
GGGCTGATGGTGGTGGTTATCACCACGTGCCTTGCCGTGACACGGGTGTATGGCATTCAG
AGGTTCTGCCGAGACATCCACATGATGCTGGGCTTCAAGCCGGGCCTCTACTTCAGGGCC
TGCTGGCTGTTCCTGTCCCCAGCCACGCTCTTGGCCCTCCTGGTGTATAGCATCGTCAAG
TACCAGCCCTCGGAGTATGGCAGTTACCGCTTCCCGCCCTGGGCTGAGCTGCTGGGCATC
CTGATGGGCCTGCTGTCCTGCCTCATGATCCCAGCTGGCATGCTGGTGGCTGTGCTTCGA
GAAGAGGGCTCACTCTGGGAGCGGCTCCAACAGGCCAGCCGGCCGGCCATGGACTGGGGA
CCATCACTGGAGGAGAACCGGACGGGCATGTATGTGGCCACGCTGGCTGGGAGCCAGTCA
CCAAAGCCACTGATGGTGCACATGCGCAAGTACGGGGGCATCACCAGCTTCGAGAACACG
GCCATCGAGGTGGACCGTGAGATTGCAGAGGAGGAGGAGTCGATGATG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SLC6A7
Symbol Nomenclature : SLC6A7
SYNONYM : PROT
Designation : brain-specific L-proline transporter|sodium-dependent proline transporter|solute carrier family 6 (neurotransmitter transporter, L-proline), member 7|solute carrier family 6 member 7
Full Nomenclature : solute carrier family 6 (neurotransmitter transporter), member 7
GENEID : 6534
GenBank Accession : HQ258340
HGNC : 11054
MIM : 606205
Vega : OTTHUMG00000130046
Target GenBank: HQ258340

 

Reference Sequence Alignment

  

HsCD00398457      ATGAAGAAGCTCCAGGGAGCTCACCTCCGCAAGCCTGTCACCCCAGACCTGCTGATGACC
NM_014228.3       ATGAAGAAGCTCCAGGGAGCTCACCTCCGCAAGCCTGTCACCCCAGACCTGCTGATGACC
                  ************************************************************

HsCD00398457      CCCAGTGACCAGGGCGATGTCGACCTGGATGTGGACTTTGCTGCACACCGGGGGAACTGG
NM_014228.3       CCCAGTGACCAGGGCGATGTCGACCTGGATGTGGACTTTGCTGCACACCGGGGGAACTGG
                  ************************************************************

HsCD00398457      ACAGGCAAGCTGGACTTCCTGCTGTCCTGCATTGGCTACTGTGTAGGCCTGGGGAATGTC
NM_014228.3       ACAGGCAAGCTGGACTTCCTGCTGTCCTGCATTGGCTACTGTGTAGGCCTGGGGAATGTC
                  ************************************************************

HsCD00398457      TGGCGCTTCCCCTATCGAGCGTACACCAATGGAGGAGGCGCCTTCCTCGTGCCCTACTTC
NM_014228.3       TGGCGCTTCCCCTATCGAGCGTACACCAATGGAGGAGGCGCCTTCCTCGTGCCCTACTTC
                  ************************************************************

HsCD00398457      CTCATGCTGGCCATCTGTGGCATCCCCCTCTTCTTCCTGGAGCTCTCCCTGGGCCAGTTC
NM_014228.3       CTCATGCTGGCCATCTGTGGCATCCCCCTCTTCTTCCTGGAGCTCTCCCTGGGCCAGTTC
                  ************************************************************

HsCD00398457      TCCAGCCTAGGGCCCCTGGCTGTCTGGAAAATCAGCCCTCTCTTCAAAGGCGCCGGCGCA
NM_014228.3       TCCAGCCTAGGGCCCCTGGCTGTCTGGAAAATCAGCCCTCTCTTCAAAGGCGCCGGCGCA
                  ************************************************************

HsCD00398457      GCCATGCTGCTCATCGTGGGCTTGGTGGCCATCTACTACAACATGATCATCGCCTACGTG
NM_014228.3       GCCATGCTGCTCATCGTGGGCTTGGTGGCCATCTACTACAACATGATCATCGCCTACGTG
                  ************************************************************

HsCD00398457      CTCTTCTACCTCTTCGCCTCCCTCACCAGCGACCTACCCTGGGAGCACTGTGGCAACTGG
NM_014228.3       CTCTTCTACCTCTTCGCCTCCCTCACCAGCGACCTACCCTGGGAGCACTGTGGCAACTGG
                  ************************************************************

HsCD00398457      TGGAACACAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGGGGCCCTGCCC
NM_014228.3       TGGAACACAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGGGGCCCTGCCC
                  ************************************************************

HsCD00398457      CTCAACCTCACCTGCACCGTCAGCCCCAGCGAGGAGTACTGGAGCCGCTACGTCCTCCAC
NM_014228.3       CTCAACCTCACCTGCACCGTCAGCCCCAGCGAGGAGTACTGGAGCCGCTACGTCCTCCAC
                  ************************************************************

HsCD00398457      ATCCAAGGCAGCCAGGGCATCGGCAGCCCTGGGGAGATCCGCTGGAACCTCTGCCTCTGC
NM_014228.3       ATCCAAGGCAGCCAGGGCATCGGCAGCCCTGGGGAGATCCGCTGGAACCTCTGCCTCTGC
                  ************************************************************

HsCD00398457      CTGCTGCTGGCCTGGGTCATCGTGTTCCTCTGTATCCTCAAGGGTGTGAAGTCTTCGGGC
NM_014228.3       CTGCTGCTGGCCTGGGTCATCGTGTTCCTCTGTATCCTCAAGGGTGTGAAGTCTTCGGGC
                  ************************************************************

HsCD00398457      AAGGTGGTGTATTTCACGGCCACGTTCCCCTACCTCATCCTGCTCATGCTGCTGGTCCGC
NM_014228.3       AAGGTGGTGTATTTCACGGCCACGTTCCCCTACCTCATCCTGCTCATGCTGCTGGTCCGC
                  ************************************************************

HsCD00398457      GGAGTCACCCTCCCAGGGGCCTGGAAGGGCATCCAGTTCTATCTCACCCCCCAGTTCCAC
NM_014228.3       GGAGTCACCCTCCCAGGGGCCTGGAAGGGCATCCAGTTCTATCTCACCCCCCAGTTCCAC
                  ************************************************************

HsCD00398457      CACTTGTTGTCTTCCAAGGTGTGGATTGAAGCTGCTCTTCAGATCTTCTATTCCCTGGGT
NM_014228.3       CACTTGTTGTCTTCCAAGGTGTGGATTGAAGCTGCTCTTCAGATCTTCTATTCCCTGGGT
                  ************************************************************

HsCD00398457      GTGGGCTTCGGGGGGCTCCTCACCTTTGCCTCCTACAACACGTTTCACCAGAACATCTAT
NM_014228.3       GTGGGCTTCGGGGGGCTCCTCACCTTTGCCTCCTACAACACGTTTCACCAGAACATCTAT
                  ************************************************************

HsCD00398457      AGAGACACTTTCATCGTCACTCTGGGCAACGCCATCACCAGCATCCTGGCTGGCTTTGCC
NM_014228.3       AGAGACACTTTCATCGTCACTCTGGGCAACGCCATCACCAGCATCCTGGCTGGCTTTGCC
                  ************************************************************

HsCD00398457      ATCTTCTCCGTGCTGGGCTACATGTCTCAGGAGCTGGGCGTGCCTGTGGACCAAGTAGCC
NM_014228.3       ATCTTCTCCGTGCTGGGCTACATGTCTCAGGAGCTGGGCGTGCCTGTGGACCAAGTAGCC
                  ************************************************************

HsCD00398457      AAAGCAGGCCCTGGCCTGGCCTTTGTCGTCTACCCACAGGCCATGACCATGCTGCCTCTG
NM_014228.3       AAAGCAGGCCCTGGCCTGGCCTTTGTCGTCTACCCACAGGCCATGACCATGCTGCCTCTG
                  ************************************************************

HsCD00398457      TCACCCTTCTGGTCCTTTCTCTTCTTCTTCATGCTTCTGACTCTCGGCCTAGACAGCCAG
NM_014228.3       TCACCCTTCTGGTCCTTTCTCTTCTTCTTCATGCTTCTGACTCTCGGCCTAGATAGCCAG
                  ***************************************************** ******

HsCD00398457      TTTGCTTTTCTGGAGACCATTGTGACAGCTGTGACAGATGAGTTCCCATACTACCTGCGG
NM_014228.3       TTTGCTTTTCTGGAGACCATTGTGACAGCTGTGACAGATGAGTTCCCATACTACCTGCGG
                  ************************************************************

HsCD00398457      CCCAAGAAGGCGGTGTTCTCAGGGCTCATCTGCGTGGCCATGTACCTGATGGGGCTGATC
NM_014228.3       CCCAAGAAGGCGGTGTTCTCAGGGCTCATCTGCGTGGCCATGTACCTGATGGGGCTGATC
                  ************************************************************

HsCD00398457      CTCACCACTGATGGGGGCATGTACTGGCTGGTCCTTCTGGATGACTACAGCGCCAGCTTC
NM_014228.3       CTCACCACTGATGGGGGCATGTACTGGCTGGTCCTTCTGGATGACTACAGCGCCAGCTTC
                  ************************************************************

HsCD00398457      GGGCTGATGGTGGTGGTTATCACCACGTGCCTTGCCGTGACACGGGTGTATGGCATTCAG
NM_014228.3       GGGCTGATGGTGGTGGTTATCACCACGTGCCTTGCCGTGACACGGGTGTATGGCATTCAG
                  ************************************************************

HsCD00398457      AGGTTCTGCCGAGACATCCACATGATGCTGGGCTTCAAGCCGGGCCTCTACTTCAGGGCC
NM_014228.3       AGGTTCTGCCGAGACATCCACATGATGCTGGGCTTCAAGCCGGGCCTCTACTTCAGGGCC
                  ************************************************************

HsCD00398457      TGCTGGCTGTTCCTGTCCCCAGCCACGCTCTTGGCCCTCCTGGTGTATAGCATCGTCAAG
NM_014228.3       TGCTGGCTGTTCCTGTCCCCAGCCACGCTCTTGGCCCTCATGGTGTATAGCATCGTCAAG
                  ***************************************.********************

HsCD00398457      TACCAGCCCTCGGAGTATGGCAGTTACCGCTTCCCGCCCTGGGCTGAGCTGCTGGGCATC
NM_014228.3       TACCAGCCCTCGGAGTATGGCAGTTACCGCTTCCCGCCCTGGGCTGAGCTGCTGGGCATC
                  ************************************************************

HsCD00398457      CTGATGGGCCTGCTGTCCTGCCTCATGATCCCAGCTGGCATGCTGGTGGCTGTGCTTCGA
NM_014228.3       CTGATGGGCCTGCTGTCCTGCCTCATGATCCCAGCTGGCATGCTGGTGGCTGTGCTTCGA
                  ************************************************************

HsCD00398457      GAAGAGGGCTCACTCTGGGAGCGGCTCCAACAGGCCAGCCGGCCGGCCATGGACTGGGGA
NM_014228.3       GAAGAGGGCTCACTCTGGGAGCGGCTCCAACAGGCCAGCCGGCCGGCCATGGACTGGGGA
                  ************************************************************

HsCD00398457      CCATCACTGGAGGAGAACCGGACGGGCATGTATGTGGCCACGCTGGCTGGGAGCCAGTCA
NM_014228.3       CCATCGCTGGAGGAGAACCGGACGGGCATGTATGTGGCCACGCTGGCTGGGAGCCAGTCA
                  *****.******************************************************

HsCD00398457      CCAAAGCCACTGATGGTGCACATGCGCAAGTACGGGGGCATCACCAGCTTCGAGAACACG
NM_014228.3       CCAAAGCCACTGATGGTGCACATGCGCAAGTACGGGGGCATCACCAGCTTCGAGAACACG
                  ************************************************************

HsCD00398457      GCCATCGAGGTGGACCGTGAGATTGCAGAGGAGGAGGAGTCGATGATG---
NM_014228.3       GCCATCGAGGTGGACCGTGAGATTGCAGAGGAGGAGGAGTCGATGATGTGA
                  ************************************************   

Pathways

Reactome : Amine compound SLC transporters
Reactome : Creatine metabolism
Reactome : Metabolism
Reactome : Metabolism of amino acids and derivatives
Reactome : Na+/Cl- dependent neurotransmitter transporters
Reactome : SLC-mediated transmembrane transport
Reactome : Transmembrane transport of small molecules
Reactome : Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds

Protein

HPRD : 09371

Clone

Cloning Information : 570995

Organism


TAX_ID : 9606
Species Specific ID: 6534

Genome

Chromosome : 5
Map Location : 5q32
Ensembl : ENSG00000011083