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FNDC7 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FNDC7
Gene Name:  fibronectin type III domain containing 7
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072859)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00398698

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1500nts         Open reading frame : 1 to 1500
Reference Sequence Alignment

ATGGCTTTGAGCGACTCTTCAGAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATC
TCTTCCCTCCAGTGTGGAACTGAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGA
TCTAGCAAATCATCTTCAGCAATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTG
ACGATCCAAGAAGATCCCCCTGGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGT
GACTACTATGTGGTCTTTGTGAAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCT
CTCACTCAGTGCAACTTCTTATCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTC
TATAACAAGGCAGGGCAAAGTCCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGT
TGTCCTAGTGACATTAACCCCGTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCT
CCTGTCCGTGGTGCCGAACTGTATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAG
TGCAATGACACTACTCCTGCGTGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAAC
ATCACAGTGTATTCATTCAATGAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTC
ATAACCACAGCTCCTTGCAGTCCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATG
ATTAATGTGCACTGGCGATCCACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGG
GAGAAAGGACTGTATCAGTGCAGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCC
TGTGGCTCAGTGTTCTCTGTCACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCC
AGCTACAGTGTGCCCCTGGAAACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAA
ATCACCCAGTCAGTAATCAACGTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTT
GCAGTTCTGGAGTCACACACTGGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTC
CTAGGGTGCATCACATGTGGCATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACC
GGGTTGACTGCAGATTGCTCCTACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGG
GTGAAATTATATAGGCTGGGCCCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGC
TCTGCCAATTACAGCACTGACCTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGT
GCTGGCCTCAGTTTCTGTGATGTCACTGAGATACCCTGTGGGGATGTATACACTGTGATG
GTCTCACCAGTTGCTAAAACAGGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCA
GTAACTTGCTCTGGAAGTACACTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FNDC7
Symbol Nomenclature : FNDC7
SYNONYM : RP11-293A10.2
Designation : fibronectin type III domain-containing protein 7
Full Nomenclature : fibronectin type III domain containing 7
GENEID : 163479
GenBank Accession : HQ258430
HGNC : 26668
Vega : OTTHUMG00000011120
Target GenBank: HQ258430

 

Reference Sequence Alignment

  

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      ATGGCTGGTGGACGAGAGACATGTTTGCCTTTGATTGGATTCATTCTTATCTGTCTTAAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      ATGGTTGCTTCAGCAAAATCAGCTCCTGAAATACCCACTATTGATCAGGCATATTCAAAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      CTCAGCAACAGTATCACTGTAGAATGGGCTACAGTGCCGGGTGCCACCAGTTACCTCCTC
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      ACGGCTGAAGACGGGGACACAGTCATTGAAACCACGGTGGCCAATTCCCCAGGCACTGTG
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      ACGGGACTAAAGGCTGCAACCTGGTATGAAATCACCATCAGATCCATCAGCGCTGCTGGG
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      AGAAGCCAGGCGTCACCTCCAAAGCAGGCAAAGACAGTGTTGGCTGCACCAATTCTAGAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      GTAAGCTCTCCAAGTTCAGACTCCATTCTTGTGCAGTGGGAAGCTGTATATATGGCAATT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      GCATTCTCCGTGTCCATTATGCGAGCCAATGGCTTGGGGAGTATATGGAAAGAGAATACT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      ACAAACACCTCCTTGACATTCACCAGTTTAGAAGCCGGAACTCTCTACACCATAAAGGCC
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      TATGCATGGAATGCCAACAGAATCCCTGGGGATGACTCCACCTGCAATCAGAGAACAAGT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ------------------------------------------------------------
NM_001144937.1      CCTCGGGCCCCTGCCAACATTCAAGTCTCTTTCGATAGTGGAGCTCTGAAGGCATCTTTT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00398698        ---------------------------------------ATGGCTTTGAGCGACTCTTCA
NM_001144937.1      TCCTGGGCACGGGCAGAAGGAGCTTTCAATTATACTGTGATGGCTTTGAGCGACTCTTCA
XM_005270550.1      ---------------------------------------ATGGCTTTGAGCGACTCTTCA
                                                           *********************

HsCD00398698        GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
NM_001144937.1      GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
XM_005270550.1      GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
                    ************************************************************

HsCD00398698        GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
NM_001144937.1      GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
XM_005270550.1      GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
                    ************************************************************

HsCD00398698        ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
NM_001144937.1      ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
XM_005270550.1      ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
                    ************************************************************

HsCD00398698        GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
NM_001144937.1      GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
XM_005270550.1      GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
                    ************************************************************

HsCD00398698        AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
NM_001144937.1      AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
XM_005270550.1      AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
                    ************************************************************

HsCD00398698        TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
NM_001144937.1      TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
XM_005270550.1      TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
                    ************************************************************

HsCD00398698        CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
NM_001144937.1      CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
XM_005270550.1      CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
                    ************************************************************

HsCD00398698        GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
NM_001144937.1      GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
XM_005270550.1      GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
                    ************************************************************

HsCD00398698        TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
NM_001144937.1      TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
XM_005270550.1      TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
                    ************************************************************

HsCD00398698        TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
NM_001144937.1      TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
XM_005270550.1      TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
                    ************************************************************

HsCD00398698        GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
NM_001144937.1      GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
XM_005270550.1      GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
                    ************************************************************

HsCD00398698        CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
NM_001144937.1      CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
XM_005270550.1      CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
                    ************************************************************

HsCD00398698        ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
NM_001144937.1      ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
XM_005270550.1      ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
                    ************************************************************

HsCD00398698        AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
NM_001144937.1      AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
XM_005270550.1      AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
                    ************************************************************

HsCD00398698        ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
NM_001144937.1      ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
XM_005270550.1      ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
                    ************************************************************

HsCD00398698        ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
NM_001144937.1      ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
XM_005270550.1      ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
                    ************************************************************

HsCD00398698        GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
NM_001144937.1      GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
XM_005270550.1      GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
                    ************************************************************

HsCD00398698        GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGGTGCATCACATGTGGC
NM_001144937.1      GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGATGCATCACATGTGGC
XM_005270550.1      GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGATGCATCACATGTGGC
                    ********************************************.***************

HsCD00398698        ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
NM_001144937.1      ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
XM_005270550.1      ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
                    ************************************************************

HsCD00398698        TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
NM_001144937.1      TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
XM_005270550.1      TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
                    ************************************************************

HsCD00398698        CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
NM_001144937.1      CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
XM_005270550.1      CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
                    ************************************************************

HsCD00398698        CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
NM_001144937.1      CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
XM_005270550.1      CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
                    ************************************************************

HsCD00398698        GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
NM_001144937.1      GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
XM_005270550.1      GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
                    ************************************************************

HsCD00398698        GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTAACTTGCTCTGGAAGTACA
NM_001144937.1      GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTAACTTGCTCTGGAAGTACA
XM_005270550.1      GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTGATTTATAGAGGGAAGAGA
                    *****************************************.* **. : :**.*. * *

HsCD00398698        CTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAA---
NM_001144937.1      CTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAATGA
XM_005270550.1      AATGAAGAATGA------------------------------
                    .:**.*.:.  .                              

Protein

SMART domain : FN3 : Fibronectin type 3 domain
HPRD : 08195

Clone

Cloning Information : 571236

Organism


TAX_ID : 9606
Species Specific ID: 163479

Genome

Chromosome : 1
Map Location : 1p13.3
Ensembl : ENSG00000143107

Reagents

Labome : FNDC7-antibody