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RNASE12 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RNASE12
Gene Name:  ribonuclease, RNase A family, 12 (non-active)
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100072391)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00398978

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 441nts         Open reading frame : 1 to 441
Reference Sequence Alignment

ATGATAATAATGGTGATAATTTTCTTGGTGCTTCTGTTCTGGGAAAATGAGGTGAATGAT
GAAGCAGTGATGTCAACTTTAGAACACTTGCATGTGGACTACCCTCAGAATGACGTTCCC
GTTCCTGCAAGGTACTGCAACCACATGATCATACAAAGAGTTATCAGGGAACCTGACCAC
ACTTGTAAAAAGGAGCATGTCTTCATCCATGAGAGGCCTCGAAAAATCAATGGTATTTGC
ATTTCTCCCAAGAAGGTTGCTTGCCAAAACCTTTCGGCCATTTTCTGCTTTCAGAGTGAG
ACAAAGTTCAAAATGACAGTCTGTCAGCTCATTGAAGGCACAAGATACCCTGCCTGCAGG
TACCACTATTCCCCCACAGAGGGGTTTGTTCTTGTCACTTGTGATGACTTGAGGCCAGAT
AGTTTCCTTGGCTATGTTAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNASE12
Symbol Nomenclature : RNASE12
SYNONYM : HEL-S-85p; RAI1
Designation : epididymis secretory sperm binding protein Li 85p|probable inactive ribonuclease-like protein 12|ribonuclease A I1|ribonuclease-like protein 12
Full Nomenclature : ribonuclease, RNase A family, 12 (non-active)
GENEID : 493901
GenBank Accession : HQ258082
HGNC : 24211
Vega : OTTHUMG00000170991
Target GenBank: HQ258082

 

Reference Sequence Alignment

  

HsCD00398978        ATGATAATAATGGTGATAATTTTCTTGGTGCTTCTGTTCTGGGAAAATGAGGTGAATGAT
NM_001024822.2      ATGATAATAATGGTGATAATTTTCTTGGTGCTTCTGTTCTGGGAAAATGAGGTGAATGAT
                    ************************************************************

HsCD00398978        GAAGCAGTGATGTCAACTTTAGAACACTTGCATGTGGACTACCCTCAGAATGACGTTCCC
NM_001024822.2      GAAGCAGTGATGTCAACTTTAGAACACTTGCATGTGGACTACCCTCAGAATGACGTTCCC
                    ************************************************************

HsCD00398978        GTTCCTGCAAGGTACTGCAACCACATGATCATACAAAGAGTTATCAGGGAACCTGACCAC
NM_001024822.2      GTTCCTGCAAGGTACTGCAACCACATGATCATACAAAGAGTTATCAGGGAACCTGACCAC
                    ************************************************************

HsCD00398978        ACTTGTAAAAAGGAGCATGTCTTCATCCATGAGAGGCCTCGAAAAATCAATGGTATTTGC
NM_001024822.2      ACTTGTAAAAAGGAGCATGTCTTCATCCATGAGAGGCCTCGAAAAATCAATGGTATTTGC
                    ************************************************************

HsCD00398978        ATTTCTCCCAAGAAGGTTGCTTGCCAAAACCTTTCGGCCATTTTCTGCTTTCAGAGTGAG
NM_001024822.2      ATTTCTCCCAAGAAGGTTGCTTGCCAAAACCTTTCGGCCATTTTCTGCTTTCAGAGTGAG
                    ************************************************************

HsCD00398978        ACAAAGTTCAAAATGACAGTCTGTCAGCTCATTGAAGGCACAAGATACCCTGCCTGCAGG
NM_001024822.2      ACAAAGTTCAAAATGACAGTCTGTCAGCTCATTGAAGGCACAAGATACCCTGCCTGCAGG
                    ************************************************************

HsCD00398978        TACCACTATTCCCCCACAGAGGGGTTTGTTCTTGTCACTTGTGATGACTTGAGGCCAGAT
NM_001024822.2      TACCACTATTCCCCCACAGAGGGGTTTGTTCTTGTCACTTGTGATGACTTGAGGCCAGAT
                    ************************************************************

HsCD00398978        AGTTTCCTTGGCTATGTTAAA---
NM_001024822.2      AGTTTCCTTGGCTATGTTAAATAA
                    *********************   

Protein

SMART domain : RNAse_Pc : Pancreatic ribonuclease
HPRD : 18667

Clone

Cloning Information : 571516

Organism


TAX_ID : 9606
Species Specific ID: 493901

Genome

Chromosome : 14
Map Location : 14q11.1
Ensembl : ENSG00000258436

Reagents

Labome : RNASE12-antibody