Gene:
Gene Symbol:
NLRC5
Gene Name:
NLR family, CARD domain containing 5
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
The ORFeome Collaboration entry clone (IMAGE:100069204)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00399102
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGACCCCGTTGGCCTCCAGCTCGGCAACAAGAACCTGTGGAGCTGTCTTGTGAGGCTG
CTCACCAAAGACCCAGAATGGCTGAACGCCAAGATGAAGTTCTTCCTCCCCAACACGGAC
CTGGATTCCAGGAACGAGACCTTGGACCCTGAACAGAGAGTCATCCTGCAACTCAACAAG
CTGCATGTCCAGGGTTCGGACACCTGGCAGTCTTTCATTCATTGTGTGTGCATGCAGCTG
GAGGTGCCTCTGGACCTGGAGGTGCTGCTGCTGAGTACTTTTGGCTATGATGATGGGTTC
ACCAGCCAGCTGGGAGCTGAGGGGAAAAGCCAACCTGAATCTCAGCTCCACCATGGCCTG
AAGCGCCCACATCAGAGCTGTGGGTCCTCACCCCGCCGGAAGCAGTGCAAGAAGCAGCAG
CTAGAGTTGGCCAAGAAGTACCTGCAGCTCCTGCGGACCTCTGCCCAGCAGCGCTACAGG
AGCCAAATCCCTGGGTCAGGGCAGCCCCACGCCTTCCACCAGGTCTATGTCCCTCCAATC
CTGCGCCGGGCCACAGCATCCTTAGACACTCCGGAGGGGGCCATTATGGGGGACGTCAAG
GTGGAAGATGGTGCTGACGTGAGCATCTCGGACCTCTTCAACACCAGGGTTAACAAGGGC
CCGAGGGTGACCGTGCTTTTGGGGAAGGCTGGCATGGGCAAGACCACGCTGGCCCACCGG
CTCTGCCAGAAGTGGGCAGAGGGCCATCTGAACTGTTTCCAGGCCCTGTTCCTTTTTGAA
TTCCGCCAGCTCAACTTGATCACGAGGTTCCTGACACCGTCCGAGCTCCTTTTTGATCTG
TACCTGAGCCCTGAATCGGACCACGACACTGTCTTCCAGTACCTGGAGAAGAACGCTGAC
CAAGTCCTGCTGATCTTTGATGGGCTAGATGAGGCCCTCCAGCCTATGGGTCCTGATGGC
CCAGGCCCAGTCCTCACCCTTTTCTCCCATCTCTGCAATGGGACCCTCCTGCCTGGCTGC
CGGGTGATGGCTACCTCCCGTCCAGGGAAGCTGCCTGCCTGCCTGCCTGCAGAGGCAGCC
ATGGTCCACATGTTGGGCTTTGATGGGCCACGGGTGGAAGAATATGTGAATCACTTCTTC
AGCGCCCAGCCATCGCGGGAGGGGGCCCTGGTGGAGTTACAGACAAATGGACGTCTCCGA
AGCCTGTGTGCGGTGCCCGCACTGTGCCAAGTCGCCTGTCTCTGCCTCCACCATCTGCTT
CCTGACCACGCCCCAGGCCAGTCTGTGGCCCTCCTGCCCAACATGACTCAGCTCTATATG
CAGATGGTGCTCGCCCTCAGCCCCCCTGGGCACTTGCCCACCTCGTCCCTACTGGACCTG
GGGGAGGTGGCCCTGAGGGGCCTGGAGACAGGGAAGGTTATCTTCTATGCAAAAGATATT
GCTCCACCCTTGATAGCTTTTGGGGCCACTCACAGCCTGCTGACTTCCTTCTGCGTCTGC
ACAGGCCCTGGGCACCAGCAGACAGGCTATGCTTTCACCCACCTCAGCCTGCAGGAGTTT
CTTGCTGCCCTGCACCTGATGGCCAGCCCCAAGGTGAACAAAGACACACTTACCCAGTAT
GTTACCCTCCATTCCCGCTGGGTACAGCGGACCAAAGCTAGACTGGGCCTCTCAGACCAC
CTCCCCACCTTCCTGGCGGGCCTGGCATCCTGCACCTGCCGCCCCTTCCTTAGCCACCTG
GCGCAGGGCAATGAGGACTGTGTGGGTGCCAAGCAGGCTGCTGTAGTGCAGGTGTTGAAG
AAGTTGGCCACCCGCAAGCTCACAGGGCCAAAGGTTGTAGAGCTGTGTCACTGTGTGGAT
GAGACACAGGAGCCTGAGCTGGCCAGTCTCACCGCACAAAGCCTCCCCTATCAACTGCCC
TTCCACAATTTCCCACTGACCTGCACCGACCTGGCCACCCTGACCAACATCCTAGAGCAC
AGGGAGGCCCCCATCCACCTGGATTTTGATGGCTGTCCCCTGGAGCCCCACTGCCCTGAG
GCTCTGGTAGGCTGTGGGCAGATAGAGAATCTCAGCTTTAAGAGCAGGAAGTGTGGGGAT
GCCTTTGCAGAAGCCCTCTCCAGGAGCTTGCCGACAATGGGGAGGCTGCAGATGCTGGGG
TTAGCAGGAAGTAAAATCACTGCCCGAGGCATCAGCCACCTGGTGAAAGCTTTGCCTCTC
TGTCCACAGCTGAAAGAAGTCAGTTTTCGGGACAACCAGCTCAGTGACCAGGTGGTGCTG
AACATTGTGGAGGTTCTCCCTCACCTACCACGGCTCCGGAAGCTTGACCTGAGCAGCAAC
AGCATCTGCGTGTCAACCCTACTCTGCTTGGCAAGGGTGGCAGTCACGTGTCCTACCGTC
AGGATGCTTCAGGCCAGGGAGGCGGACCTCATCTTCCTTCTTTCCCCGCCCACAGAGACA
ACTGCAGAGCTACAAAGAGCTCCAGACCTGCAGGAAAGTGACGGCCAGAGGAAAGGGGCT
CAGAGCAGAAGCTTGACGCTCAGGCTGCAGAAGTGTCAGCTCCAGGTCCACGATGCGGAG
GCCCTCATAGCCCTGCTCCAGGAAGGCCCTCACCTGGAGGAAGTGGACCTCTCAGGGAAC
CAGCTGGAAGATGAAGGCTGTCGGCTGATGGCAGAGGCTGCATCCCAGCTGCACATCGCC
AGGAAGCTGGACCTCAGTGACAACGGGCTTTCTGTGGCCGGGGTGCATTGTGTGCTGAGG
GCCGTGAGTGCGTGCTGGACCCTGGCAGAGCTGCACATCAGCCTGCAGCACAAAACTGTG
ATCTTCATGTTTGCCCAGGAGCCAGAGGAGCAGAAGGGGCCCCAGGAGAGGGCTGCATTT
CTTGACAGCCTCATGCTCCAGATGCCCTCTGAGCTGCCTCTGAGCTCCCGAAGGATGAGG
CTGACACATTGTGGCCTCCAAGAAAAGCACCTAGAGCAGCTCTGCAAGGCTCTGGGAGGA
AGCTGCCACCTCGGTCACCTCCACCTCGACTTCTCAGGCAATGCTCTGGGGGATGAAGGT
GCAGCCCGGCTGGCTCAGCTGCTCCCAGGGCTGGGAGCTCTGCAGTCCTTGAACCTCAGT
GAGAACGGTTTGTCCCTGGATGCCGTGTTGGGTTTGGTTCGGTGCTTCTCCACTCTGCAG
TGGCTCTTCCGCTTGGACATCAGCTTTGAAAGCCAACACATCCTCCTGAGAGGGGACAAG
ACAAGCAGGGATATGTGGGCCACTGGATCTTTGCCAGACTTCCCAGCTGCAGCCAAGTTC
TTAGGGTTCCGTCAGCGCTGCATCCCCAGGAGCCTCTGCCTCAGTGAGTGTCCTCTGGAG
CCCCCAAGCCTCACCCGCCTCTGTGCCACTCTGAAGGACTGCCCGGGACCCCTGGAACTG
CAATTGTCCTGTGAGTTCCTGAGTGACCAGAGCCTGGAGACTCTACTGGACTGCTTACCT
CAACTCCCTCAGCTGAGCCTGCTGCAGCTGAGCCAGACGGGACTGTCCCCGAAAAGCCCC
TTCCTGCTGGCCAACACCTTAAGCCTGTGTCCACGGGTTAAAAAGGTGGATCTCAGGTCC
CTGCACCATGCAACTTTGCACTTCAGATCCAACGAGGAGGAGGAAGGCGTGTGCTGTGGC
AGGTTCACAGGCTGCAGCCTCAGCCAGGAGCACGTAGAGTCACTCTGCTGGTTGCTGAGC
AAGTGTAAAGACCTCAGCCAGGTGGATCTCTCAGCAAACCTGCTGGGCGACAGCGGACTC
AGATGCCTTCTGGAATGTCTGCCGCAGGTGCCCATCTCCGGTTTGCTTGATCTGAGTCAC
AACAGCATTTCTCAGGAAAGTGCCCTGTACCTGCTGGAGACACTGCCCTCCTGCCCACGT
GTCCGGGAGGCCTCAGTGAACCTGGGCTCTGAGCAGAGCTTCCGGATTCACTTCTCCAGA
GAGGACCAGGCTGGGAAGACACTCAGGCTAAGTGAGTGCAGCTTCCGGCCAGAGCACGTG
TCCAGGCTGGCCACCGGCTTGAGCAAGTCCCTGCAGCTGACGGAGCTCACGCTGACCCAG
TGCTGCCTGGGCCAGAAGCAGCTGGCCATCCTCCTGAGCTTGGTGGGGCGACCCGCAGGG
CTGTTCAGCCTCAGGGTGCAGGAGCCGTGGGCGGACAGAGCCAGGGTTCTCTCCCTGTTA
GAAGTCTGCGCCCAGGCCTCAGGCAGTGTCACTGAAATCAGCATCTCCGAGACCCAGCAG
CAGCTCTGTGTCCAGCTGGAATTTCCTCGCCAGGAAGAGAATCCAGAAGCTGTGGCACTC
AGGTTGGCTCACTGTGACCTTGGAGCCCACCACAGCCTTCTTGTCGGGCAGCTGATGGAG
ACATGTGCCAGGCTGCAGCAGCTCAGCTTGTCTCAGGTTAACCTCTGTGAGGACGATGAT
GCCAGTTCCCTGCTGCTGCAGAGCCTCCTGCTGTCCCTCTCTGAGCTGAAGACATTTCGG
CTGACCTCCAGCTGTGTGAGCACCGAGGGCCTCGCCCACCTGGCATCTGGTCTGGGCCAC
TGCCACCACTTGGAGGAGCTGGACTTGTCTAACAATCAATTTGATGAGGAGGGCACCAAG
GCGCTGATGAGGGCCCTTGAGGGGAAATGGATGCTAAAGAGGCTGGACCTCAGTCACCTT
CTGCTGAACAGCTCCACCTTGGCCTTGCTTACTCACAGACTAAGCCAGATGACCTGCCTG
CAGAGCCTCAGACTGAACAGGAACAGTATCGGTGATGTCGGTTGCTGCCACCTTTCTGAG
GCTCTCAGGGCTGCCACCAGCCTAGAGGAGCTGGACTTGAGCCACAACCAGATTGGAGAC
GCTGGTGTCCAGCACTTAGCTACCATCCTGCCTGGGCTGCCAGAGCTCAGGAAGATAGAC
CTCTCAGGGAATAGCATCAGCTCAGCCGGGGGAGTGCAGTTGGCAGAGTCTCTCGTTCTT
TGCAGGCGCCTGGAGGAGTTGATGCTTGGCTGCAATGCCCTGGGGGATCCCACAGCCCTG
GGGCTGGCTCAGGAGCTGCCCCAGCACCTGAGGGTCCTACACCTACCATTCAGCCATCTG
GGCCCAGGTGGGGCCCTGAGCCTGGCCCAGGCCCTGGATGGATCCCCCCATTTGGAAGAG
ATCAGCTTGGCGGAAAACAACCTGGCTGGAGGGGTCCTGCGTTTCTGTATGGAGCTCCCG
CTGCTCAGACAGATAGACCTGGTTTCCTGTAAGATTGACAACCAGACTGCCAAGCTCCTC
ACCTCCAGCTTCACGAGCTGCCCTGCCCTGGAAGTAATCTTGCTGTCCTGGAATCTCCTC
GGGGATGAGGCAGCTGCCGAGCTGGCCCAGGTGCTGCCGCAGATGGGCCGGCTGAAGAGA
GTGGACCTGGAGAAGAATCAGATCACAGCTTTGGGGGCCTGGCTCCTGGCTGAAGGACTG
GCCCAGGGGTCTAGCATCCAAGTCATCCGCCTCTGGAATAACCCCATTCCCTGCGACATG
GCCCAGCACCTGAAGAGCCAGGAGCCCAGGCTGGACTTTGCCTTCTTTGACAACCAGCCC
CAGGCCCCTTGGGGTACT
Protein sequence predicted based on nucleotide sequence
MDPVGLQLGNKNLWSCLVRLLTKDPEWLNAKMKFFLPNTDLDSRNETLDPEQRVILQLNK LHVQGSDTWQSFIHCVCMQLEVPLDLEVLLLSTFGYDDGFTSQLGAEGKSQPESQLHHGL KRPHQSCGSSPRRKQCKKQQLELAKKYLQLLRTSAQQRYRSQIPGSGQPHAFHQVYVPPI LRRATASLDTPEGAIMGDVKVEDGADVSISDLFNTRVNKGPRVTVLLGKAGMGKTTLAHR LCQKWAEGHLNCFQALFLFEFRQLNLITRFLTPSELLFDLYLSPESDHDTVFQYLEKNAD QVLLIFDGLDEALQPMGPDGPGPVLTLFSHLCNGTLLPGCRVMATSRPGKLPACLPAEAA MVHMLGFDGPRVEEYVNHFFSAQPSREGALVELQTNGRLRSLCAVPALCQVACLCLHHLL PDHAPGQSVALLPNMTQLYMQMVLALSPPGHLPTSSLLDLGEVALRGLETGKVIFYAKDI APPLIAFGATHSLLTSFCVCTGPGHQQTGYAFTHLSLQEFLAALHLMASPKVNKDTLTQY VTLHSRWVQRTKARLGLSDHLPTFLAGLASCTCRPFLSHLAQGNEDCVGAKQAAVVQVLK KLATRKLTGPKVVELCHCVDETQEPELASLTAQSLPYQLPFHNFPLTCTDLATLTNILEH REAPIHLDFDGCPLEPHCPEALVGCGQIENLSFKSRKCGDAFAEALSRSLPTMGRLQMLG LAGSKITARGISHLVKALPLCPQLKEVSFRDNQLSDQVVLNIVEVLPHLPRLRKLDLSSN SICVSTLLCLARVAVTCPTVRMLQAREADLIFLLSPPTETTAELQRAPDLQESDGQRKGA QSRSLTLRLQKCQLQVHDAEALIALLQEGPHLEEVDLSGNQLEDEGCRLMAEAASQLHIA RKLDLSDNGLSVAGVHCVLRAVSACWTLAELHISLQHKTVIFMFAQEPEEQKGPQERAAF LDSLMLQMPSELPLSSRRMRLTHCGLQEKHLEQLCKALGGSCHLGHLHLDFSGNALGDEG AARLAQLLPGLGALQSLNLSENGLSLDAVLGLVRCFSTLQWLFRLDISFESQHILLRGDK TSRDMWATGSLPDFPAAAKFLGFRQRCIPRSLCLSECPLEPPSLTRLCATLKDCPGPLEL QLSCEFLSDQSLETLLDCLPQLPQLSLLQLSQTGLSPKSPFLLANTLSLCPRVKKVDLRS LHHATLHFRSNEEEEGVCCGRFTGCSLSQEHVESLCWLLSKCKDLSQVDLSANLLGDSGL RCLLECLPQVPISGLLDLSHNSISQESALYLLETLPSCPRVREASVNLGSEQSFRIHFSR EDQAGKTLRLSECSFRPEHVSRLATGLSKSLQLTELTLTQCCLGQKQLAILLSLVGRPAG LFSLRVQEPWADRARVLSLLEVCAQASGSVTEISISETQQQLCVQLEFPRQEENPEAVAL RLAHCDLGAHHSLLVGQLMETCARLQQLSLSQVNLCEDDDASSLLLQSLLLSLSELKTFR LTSSCVSTEGLAHLASGLGHCHHLEELDLSNNQFDEEGTKALMRALEGKWMLKRLDLSHL LLNSSTLALLTHRLSQMTCLQSLRLNRNSIGDVGCCHLSEALRAATSLEELDLSHNQIGD AGVQHLATILPGLPELRKIDLSGNSISSAGGVQLAESLVLCRRLEELMLGCNALGDPTAL GLAQELPQHLRVLHLPFSHLGPGGALSLAQALDGSPHLEEISLAENNLAGGVLRFCMELP LLRQIDLVSCKIDNQTAKLLTSSFTSCPALEVILLSWNLLGDEAAAELAQVLPQMGRLKR VDLEKNQITALGAWLLAEGLAQGSSIQVIRLWNNPIPCDMAQHLKSQEPRLDFAFFDNQP QAPWGT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
