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IRAK3 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  IRAK3
Gene Name:  interleukin-1 receptor-associated kinase 3
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073576)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399341

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1788nts         Open reading frame : 51 to 1838
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCGGGGA
ACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGC
TGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGCTGGCGCGGCC
TGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACC
AAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCG
GTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTACAA
ACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATA
TATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCTGAACATAATG
AAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAA
ATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGA
TTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGA
AGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACA
TACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACA
TGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTT
GGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACG
TTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATC
AGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATC
AGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAG
AGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAA
TAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGC
GGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAG
ATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCC
GGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTG
AAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCA
GGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAA
GCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGA
AAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAG
AGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGT
TCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTT
CTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCA
AATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATACCCAACTTTCTTGTACAAAG
TTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : IRAK3
Symbol Nomenclature : IRAK3
SYNONYM : ASRT5; IRAKM
Designation : IL-1 receptor-associated kinase M
Full Nomenclature : interleukin-1 receptor-associated kinase 3
GENEID : 11213
GenBank Accession : JF432379
HGNC : 17020
MIM : 604459
Vega : OTTHUMG00000169002
Target GenBank: JF432379

 

Reference Sequence Alignment

  

HsCD00399341      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCGGGGA
NM_007199.2       --------------------------------------------------ATGGCGGGGA
                                                                    **********

HsCD00399341      ACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGC
NM_007199.2       ACTGTGGGGCCCGCGGCGCGCTGTCGGCGCACACGCTGCTGTTCGACCTGCCGCCCGCGC
                  ************************************************************

HsCD00399341      TGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGCTGGCGCGGCC
NM_007199.2       TGCTCGGAGAGCTCTGCGCTGTTCTGGACAGCTGCGACGGCGCGCTGGGCTGGCGCGGCC
                  ************************************************************

HsCD00399341      TGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACC
NM_007199.2       TGGCAGAGAGACTTTCAAGCAGCTGGCTGGATGTTCGTCATATTGAAAAGTATGTAGACC
                  ************************************************************

HsCD00399341      AAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCG
NM_007199.2       AAGGTAAAAGTGGAACAAGAGAATTACTTTGGTCCTGGGCACAGAAAAACAAGACCATCG
                  ************************************************************

HsCD00399341      GTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTACAA
NM_007199.2       GTGACCTTTTACAGGTCCTCCAGGAGATGGGACATCGTCGAGCTATTCATTTAATTACAA
                  ************************************************************

HsCD00399341      ACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATA
NM_007199.2       ACTATGGAGCAGTGTTGAGTCCTTCAGAGAAGAGTTATCAGGAAGGTGGATTTCCAAATA
                  ************************************************************

HsCD00399341      TATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCTGAACATAATG
NM_007199.2       TATTATTCAAGGAAACAGCCAATGTCACCGTGGATAATGTTCTTATTCCTGAACATAATG
                  ************************************************************

HsCD00399341      AAAAAGGAGTACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAA
NM_007199.2       AAAAAGGAATACTGCTTAAATCTTCCATCAGCTTTCAAAATATCATAGAAGGAACTAGAA
                  ********.***************************************************

HsCD00399341      ATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGA
NM_007199.2       ATTTCCACAAAGACTTCCTAATTGGAGAAGGAGAGATTTTTGAGGTATACAGAGTGGAGA
                  ************************************************************

HsCD00399341      TTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGA
NM_007199.2       TTCAAAACCTAACATATGCTGTCAAATTATTTAAACAGGAGAAAAAAATGCAGTGTAAGA
                  ************************************************************

HsCD00399341      AGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACA
NM_007199.2       AGCATTGGAAGAGGTTTTTATCTGAGCTTGAAGTTTTACTACTGTTTCATCACCCAAACA
                  ************************************************************

HsCD00399341      TACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACA
NM_007199.2       TACTAGAGTTGGCTGCATATTTTACAGAGACTGAGAAGTTCTGTCTGATTTATCCATACA
                  ************************************************************

HsCD00399341      TGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTT
NM_007199.2       TGAGAAATGGAACACTTTTTGACAGATTGCAGTGTGTAGGTGACACGGCCCCACTCCCTT
                  ************************************************************

HsCD00399341      GGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACG
NM_007199.2       GGCACATTCGAATCGGTATATTAATAGGAATATCCAAAGCCATTCACTACCTGCACAACG
                  ************************************************************

HsCD00399341      TTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATC
NM_007199.2       TTCAACCATGCTCGGTCATCTGTGGCAGTATATCAAGTGCAAACATCCTTTTGGATGATC
                  ************************************************************

HsCD00399341      AGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATC
NM_007199.2       AGTTTCAACCCAAACTAACTGATTTTGCCATGGCACACTTCCGGTCCCACCTAGAACATC
                  ************************************************************

HsCD00399341      AGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAG
NM_007199.2       AGAGTTGTACCATAAATATGACCAGCAGCAGCAGTAAACATCTGTGGTACATGCCAGAAG
                  ************************************************************

HsCD00399341      AGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAA
NM_007199.2       AGTACATCAGACAGGGGAAACTTTCCATTAAAACAGATGTCTACAGCTTTGGAATTGTAA
                  ************************************************************

HsCD00399341      TAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGC
NM_007199.2       TAATGGAAGTTCTAACAGGATGTAGAGTAGTGTTAGATGATCCAAAACATATCCAGCTGC
                  ************************************************************

HsCD00399341      GGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAG
NM_007199.2       GGGATCTCCTTAGAGAATTGATGGAGAAGAGAGGCCTGGATTCATGTCTCTCATTTCTAG
                  ************************************************************

HsCD00399341      ATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCC
NM_007199.2       ATAAGAAAGTGCCTCCCTGCCCTCGGAATTTCTCTGCCAAGCTCTTCTGTTTGGCAGGCC
                  ************************************************************

HsCD00399341      GGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTG
NM_007199.2       GGTGTGCTGCAACGCGGGCAAAGTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTG
                  ************************************************************

HsCD00399341      AAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCA
NM_007199.2       AAAGTACTCAAGCCAGCTTGTATTTTGCTGAAGATCCTCCCACATCACTAAAGTCCTTCA
                  ************************************************************

HsCD00399341      GGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAA
NM_007199.2       GGTGTCCTTCTCCTCTATTCCTGGAGAATGTACCAAGTATTCCAGTGGAAGATGATGAAA
                  ************************************************************

HsCD00399341      GCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGA
NM_007199.2       GCCAGAATAACAATTTACTACCTTCTGATGAAGGCCTGAGGATAGACAGAATGACTCAGA
                  ************************************************************

HsCD00399341      AAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAG
NM_007199.2       AAACTCCTTTTGAATGCAGCCAGTCTGAGGTTATGTTTCTGAGCTTGGACAAAAAGCCAG
                  ************************************************************

HsCD00399341      AGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGT
NM_007199.2       AGAGCAAGAGAAATGAGGAAGCTTGCAACATGCCCAGTTCTTCTTGTGAAGAAAGTTGGT
                  ************************************************************

HsCD00399341      TCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTT
NM_007199.2       TCCCAAAGTATATAGTTCCATCCCAGGACTTAAGGCCCTATAAGGTAAATATAGATCCTT
                  ************************************************************

HsCD00399341      CTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCA
NM_007199.2       CTTCAGAAGCTCCAGGGCATTCTTGCAGGAGCAGGCCAGTGGAGAGCAGCTGTTCCTCCA
                  ************************************************************

HsCD00399341      AATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATACCCAACTTTCTTGTACAAAG
NM_007199.2       AATTTTCCTGGGATGAATATGAACAGTACAAAAAAGAATAA-------------------
                  ****************************************.                   

HsCD00399341      TTGGCATTATAAGAAAGCATTGCTTATCAA
NM_007199.2       ------------------------------
                                                

Pathways

NCI : IL1-mediated signaling events
Panther : Toll receptor signaling pathway
Reactome : Activated TLR4 signalling
Reactome : Cytokine Signaling in Immune system
Reactome : Immune System
Reactome : Innate Immune System
Reactome : Interleukin-1 signaling
Reactome : MyD88:Mal cascade initiated on plasma membrane
Reactome : Signaling by Interleukins
Reactome : Toll Like Receptor 2 (TLR2) Cascade
Reactome : Toll Like Receptor 4 (TLR4) Cascade
Reactome : Toll Like Receptor TLR1:TLR2 Cascade
Reactome : Toll Like Receptor TLR6:TLR2 Cascade
Reactome : Toll-Like Receptors Cascades
WikiPathway : Regulation of toll-like receptor signaling pathway

Clone

Cloning Information : 573439

Organism


TAX_ID : 9606
Species Specific ID: 11213

Genome

Chromosome : 12
Map Location : 12q14.3
Ensembl : ENSG00000090376

Reagents

Labome : IRAK-M-antibody