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KBTBD2 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KBTBD2
Gene Name:  kelch repeat and BTB (POZ) domain containing 2
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073577)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399349

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1869nts         Open reading frame : 51 to 1919
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGTCCACTC
AAGACGAGAGGCAGATCAATACTGAATATGCTGTGTCATTGTTGGAACAGTTGAAACTGT
TTTATGAACAGCAGTTGTTTACTGACATAGTGTTAATTGTTGAGGGCACTGAATTCCCTT
GTCATAAGATGGTTCTTGCAACATGTAGCTCTTATTTCAGGGCCATGTTTATGAGTGGAC
TAAGTGAAAGCAAACAAACCCATGTACACCTGAGGAATGTCGATGCTGCCACCTTACAGA
TAATAATAACTTATGCATACACGGGTAACTTGGCAATGAATGACAGCACTGTAGAACAGC
TTTATGAAACAGCTTGCTTCCTACAGGTAGAAGATGTGTTACAACGTTGTCGAGAATATT
TAATTAAAAAAATAAATGCAGAGAATTGTGTACGATTGTTGAGTTTTGCTGATCTCTTCA
GTTGTGAGGAATTAAAACAGAGTGCTAAAAGAATGGTGGAGCACAAGTTCACTGCTGTGT
ATCATCAGGACGCGTTCATGCAGCTGTCACATGACCTACTGATAGATATTCTCAGTAGTG
ACAATTTAAATGTAGGAAAGGAAGAAACCGTTCGAGAAGCTGCTATGCTGTGGCTAGAGT
ATAACACAGAATCACGATCCCAGTATTTGTCTTCTGTTCTTAGCCAAATCAGAATTGATG
CACTTTCAGAAGTGACACAGAGAGCTTGGTTTCAAGGTCTGCCACCCAATGATAAGTCAG
TGGTGGTTCAAGGTCTGTATAAGTCCATGCGCAAGTTTTTCAAACCAAGACTTGGGATGA
CTAAAGAGGAAATGATGATTTTCATTGAAGCATCTTCAGAAAATCCTTGTAGTCTTTACT
CTTCTGTCTGTTACAGCCCCCAAGCAGAAAAAGTTTACAAGTTATGTAGCCGACCAGCTG
ATTTGCATAAGGTTGGGACCGTTGTAACTCCTGATAATGATATCTACATAGCAGGGGGTC
AAGTTCCTCTGAAAAACACAAAAACAAATCACAGTAAAACAAGCAAACTTCAGACTGCCT
TCAGAACTGTGAATTGCTTTTATTGGTTTGATGCACAGCAAAATACCTGGTTTCCAAAGA
CCCCAATGCTTTTTGTCCGCATAAAGCCATCTTTGGTTTGCTGTGAAGGCTATATCTATG
CAATTGGAGGAGATAGCGTAGGTGGAGAACTTAATCGGAGGACCGTAGAAAGATACGACA
CTGAGAAAGATGAGTGGACGATGGTAAGCCCTTTACCTTGTGCTTGGCAATGGAGTGCAG
CAGTTGTGGTTCATGACTGCATTTATGTGATGACACTGAACCTCATGTACTGTTATTTTC
CAAGGTCTGACTCATGGGTAGAAATGGCCATGAGACAGACTAGTAGGTCCTTTGCTTCAG
CTGCAGCTTTTGGTGATAAAATTTTCTATATTGGAGGGTTGCATATTGCTACCAATTCCG
GCATAAGACTCCCCTCTGGCACTGTAGATGGGTCTTCAGTAACTGTGGAAATTTATGATG
TGAATAAAAATGAGTGGAAAATGGCAGCCAACATCCCTGCTAAGAGGTACTCTGACCCCT
GTGTTAGAGCTGTTGTGATCTCAAATTCTATATGTGTGTTTATGCGAGAAACCCACTTAA
ATGAGCGAGCTAAATACGTCACCTACCAATATGACCTGGAACTTGACCGGTGGTCTCTGC
GGCAGCATATATCTGAACGTGTACTGTGGGACTTGGGGAGAGATTTTCGATGCACTGTGG
GGAAACTCTATCCATCCTGCCTTGAAGAGTCTCCATGGAAACCACCAACTTATCTTTTTT
CAACGGATGGGACAGAAGAGTTTGAACTGGATGGAGAAATGGTTGCACTACCACCTGTAT
ACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD2
Symbol Nomenclature : KBTBD2
SYNONYM : BKLHD1
Designation : BTB and kelch domain containing 1|BTB and kelch domain-containing protein 1|kelch repeat and BTB domain-containing protein 2
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 2
GENEID : 25948
Locus Tag : CGI-73
GenBank Accession : JF432380
HGNC : 21751
Vega : OTTHUMG00000152984
Target GenBank: JF432380

 

Reference Sequence Alignment

  

HsCD00399349        TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGTCCACTC
NM_015483.2         --------------------------------------------------ATGTCCACTC
XM_005249696.1      --------------------------------------------------ATGTCCACTC
                                                                      **********

HsCD00399349        AAGACGAGAGGCAGATCAATACTGAATATGCTGTGTCATTGTTGGAACAGTTGAAACTGT
NM_015483.2         AAGACGAGAGGCAGATCAATACTGAATATGCTGTGTCATTGTTGGAACAGTTGAAACTGT
XM_005249696.1      AAGACGAGAGGCAGATCAATACTGAATATGCTGTGTCATTGTTGGAACAGTTGAAACTGT
                    ************************************************************

HsCD00399349        TTTATGAACAGCAGTTGTTTACTGACATAGTGTTAATTGTTGAGGGCACTGAATTCCCTT
NM_015483.2         TTTATGAACAGCAGTTGTTTACTGACATAGTGTTAATTGTTGAGGGCACTGAATTCCCTT
XM_005249696.1      TTTATGAACAGCAGTTGTTTACTGACATAGTGTTAATTGTTGAGGGCACTGAATTCCCTT
                    ************************************************************

HsCD00399349        GTCATAAGATGGTTCTTGCAACATGTAGCTCTTATTTCAGGGCCATGTTTATGAGTGGAC
NM_015483.2         GTCATAAGATGGTTCTTGCAACATGTAGCTCTTATTTCAGGGCCATGTTTATGAGTGGAC
XM_005249696.1      GTCATAAGATGGTTCTTGCAACATGTAGCTCTTATTTCAGGGCCATGTTTATGAGTGGAC
                    ************************************************************

HsCD00399349        TAAGTGAAAGCAAACAAACCCATGTACACCTGAGGAATGTCGATGCTGCCACCTTACAGA
NM_015483.2         TAAGTGAAAGCAAACAAACCCATGTACACCTGAGGAATGTCGATGCTGCCACCTTACAGA
XM_005249696.1      TAAGTGAAAGCAAACAAACCCATGTACACCTGAGGAATGTCGATGCTGCCACCTTACAGA
                    ************************************************************

HsCD00399349        TAATAATAACTTATGCATACACGGGTAACTTGGCAATGAATGACAGCACTGTAGAACAGC
NM_015483.2         TAATAATAACTTATGCATACACGGGTAACTTGGCAATGAATGACAGCACTGTAGAACAGC
XM_005249696.1      TAATAATAACTTATGCATACACGGGTAACTTGGCAATGAATGACAGCACTGTAGAACAGC
                    ************************************************************

HsCD00399349        TTTATGAAACAGCTTGCTTCCTACAGGTAGAAGATGTGTTACAACGTTGTCGAGAATATT
NM_015483.2         TTTATGAAACAGCTTGCTTCCTACAGGTAGAAGATGTGTTACAACGTTGTCGAGAATATT
XM_005249696.1      TTTATGAAACAGCTTGCTTCCTACAGGTAGAAGATGTGTTACAACGTTGTCGAGAATATT
                    ************************************************************

HsCD00399349        TAATTAAAAAAATAAATGCAGAGAATTGTGTACGATTGTTGAGTTTTGCTGATCTCTTCA
NM_015483.2         TAATTAAAAAAATAAATGCAGAGAATTGTGTACGATTGTTGAGTTTTGCTGATCTCTTCA
XM_005249696.1      TAATTAAAAAAATAAATGCAGAGAATTGTGTACGATTGTTGAGTTTTGCTGATCTCTTCA
                    ************************************************************

HsCD00399349        GTTGTGAGGAATTAAAACAGAGTGCTAAAAGAATGGTGGAGCACAAGTTCACTGCTGTGT
NM_015483.2         GTTGTGAGGAATTAAAACAGAGTGCTAAAAGAATGGTGGAGCACAAGTTCACTGCTGTGT
XM_005249696.1      GTTGTGAGGAATTAAAACAGAGTGCTAAAAGAATGGTGGAGCACAAGTTCACTGCTGTGT
                    ************************************************************

HsCD00399349        ATCATCAGGACGCGTTCATGCAGCTGTCACATGACCTACTGATAGATATTCTCAGTAGTG
NM_015483.2         ATCATCAGGACGCGTTCATGCAGCTGTCACATGACCTACTGATAGATATTCTCAGTAGTG
XM_005249696.1      ATCATCAGGACGCGTTCATGCAGCTGTCACATGACCTACTGATAGATATTCTCAGTAGTG
                    ************************************************************

HsCD00399349        ACAATTTAAATGTAGGAAAGGAAGAAACCGTTCGAGAAGCTGCTATGCTGTGGCTAGAGT
NM_015483.2         ACAATTTAAATGTAGAAAAGGAAGAAACCGTTCGAGAAGCTGCTATGCTGTGGCTAGAGT
XM_005249696.1      ACAATTTAAATGTAGAAAAGGAAGAAACCGTTCGAGAAGCTGCTATGCTGTGGCTAGAGT
                    ***************.********************************************

HsCD00399349        ATAACACAGAATCACGATCCCAGTATTTGTCTTCTGTTCTTAGCCAAATCAGAATTGATG
NM_015483.2         ATAACACAGAATCACGATCCCAGTATTTGTCTTCTGTTCTTAGCCAAATCAGAATTGATG
XM_005249696.1      ATAACACAGAATCACGATCCCAGTATTTGTCTTCTGTTCTTAGCCAAATCAGAATTGATG
                    ************************************************************

HsCD00399349        CACTTTCAGAAGTGACACAGAGAGCTTGGTTTCAAGGTCTGCCACCCAATGATAAGTCAG
NM_015483.2         CACTTTCAGAAGTAACACAGAGAGCTTGGTTTCAAGGTCTGCCACCCAATGATAAGTCAG
XM_005249696.1      CACTTTCAGAAGTAACACAGAGAGCTTGGTTTCAAGGTCTGCCACCCAATGATAAGTCAG
                    *************.**********************************************

HsCD00399349        TGGTGGTTCAAGGTCTGTATAAGTCCATGCGCAAGTTTTTCAAACCAAGACTTGGGATGA
NM_015483.2         TGGTGGTTCAAGGTCTGTATAAGTCCATGCCCAAGTTTTTCAAACCAAGACTTGGGATGA
XM_005249696.1      TGGTGGTTCAAGGTCTGTATAAGTCCATGCCCAAGTTTTTCAAACCAAGACTTGGGATGA
                    ****************************** *****************************

HsCD00399349        CTAAAGAGGAAATGATGATTTTCATTGAAGCATCTTCAGAAAATCCTTGTAGTCTTTACT
NM_015483.2         CTAAAGAGGAAATGATGATTTTCATTGAAGCATCTTCAGAAAATCCTTGTAGTCTTTACT
XM_005249696.1      CTAAAGAGGAAATGATGATTTTCATTGAAGCATCTTCAGAAAATCCTTGTAGTCTTTACT
                    ************************************************************

HsCD00399349        CTTCTGTCTGTTACAGCCCCCAAGCAGAAAAAGTTTACAAGTTATGTAGCCGACCAGCTG
NM_015483.2         CTTCTGTCTGTTACAGCCCCCAAGCAGAAAAAGTTTACAAGTTATGTAGCCCACCAGCTG
XM_005249696.1      CTTCTGTCTGTTACAGCCCCCAAGCAGAAAAAGTTTACAAGTTATGTAGCCCACCAGCTG
                    *************************************************** ********

HsCD00399349        ATTTGCATAAGGTTGGGACCGTTGTAACTCCTGATAATGATATCTACATAGCAGGGGGTC
NM_015483.2         ATTTGCATAAGGTTGGGACCGTTGTAACTCCTGATAATGATATCTACATAGCAGGGGGTC
XM_005249696.1      ATTTGCATAAGGTTGGGACCGTTGTAACTCCTGATAATGATATCTACATAGCAGGGGGTC
                    ************************************************************

HsCD00399349        AAGTTCCTCTGAAAAACACAAAAACAAATCACAGTAAAACAAGCAAACTTCAGACTGCCT
NM_015483.2         AAGTTCCTCTGAAAAACACAAAAACAAATCACAGTAAAACAAGCAAACTTCAGACTGCCT
XM_005249696.1      AAGTTCCTCTGAAAAACACAAAAACAAATCACAGTAAAACAAGCAAACTTCAGACTGCCT
                    ************************************************************

HsCD00399349        TCAGAACTGTGAATTGCTTTTATTGGTTTGATGCACAGCAAAATACCTGGTTTCCAAAGA
NM_015483.2         TCAGAACTGTGAATTGCTTTTATTGGTTTGATGCACAGCAAAATACCTGGTTTCCAAAGA
XM_005249696.1      TCAGAACTGTGAATTGCTTTTATTGGTTTGATGCACAGCAAAATACCTGGTTTCCAAAGA
                    ************************************************************

HsCD00399349        CCCCAATGCTTTTTGTCCGCATAAAGCCATCTTTGGTTTGCTGTGAAGGCTATATCTATG
NM_015483.2         CCCCAATGCTTTTTGTCCGCATAAAGCCATCTTTGGTTTGCTGTGAAGGCTATATCTATG
XM_005249696.1      CCCCAATGCTTTTTGTCCGCATAAAGCCATCTTTGGTTTGCTGTGAAGGCTATATCTATG
                    ************************************************************

HsCD00399349        CAATTGGAGGAGATAGCGTAGGTGGAGAACTTAATCGGAGGACCGTAGAAAGATACGACA
NM_015483.2         CAATTGGAGGAGATAGCGTAGGTGGAGAACTTAATCGGAGGACCGTAGAAAGATACGACA
XM_005249696.1      CAATTGGAGGAGATAGCGTAGGTGGAGAACTTAATCGGAGGACCGTAGAAAGATACGACA
                    ************************************************************

HsCD00399349        CTGAGAAAGATGAGTGGACGATGGTAAGCCCTTTACCTTGTGCTTGGCAATGGAGTGCAG
NM_015483.2         CTGAGAAAGATGAGTGGACGATGGTAAGCCCTTTACCTTGTGCTTGGCAATGGAGTGCAG
XM_005249696.1      CTGAGAAAGATGAGTGGACGATGGTAAGCCCTTTACCTTGTGCTTGGCAATGGAGTGCAG
                    ************************************************************

HsCD00399349        CAGTTGTGGTTCATGACTGCATTTATGTGATGACACTGAACCTCATGTACTGTTATTTTC
NM_015483.2         CAGTTGTGGTTCATGACTGCATTTATGTGATGACACTGAACCTCATGTACTGTTATTTTC
XM_005249696.1      CAGTTGTGGTTCATGACTGCATTTATGTGATGACACTGAACCTCATGTACTGTTATTTTC
                    ************************************************************

HsCD00399349        CAAGGTCTGACTCATGGGTAGAAATGGCCATGAGACAGACTAGTAGGTCCTTTGCTTCAG
NM_015483.2         CAAGGTCTGACTCATGGGTAGAAATGGCCATGAGACAGACTAGTAGGTCCTTTGCTTCAG
XM_005249696.1      CAAGGTCTGACTCATGGGTAGAAATGGCCATGAGACAGACTAGTAGGTCCTTTGCTTCAG
                    ************************************************************

HsCD00399349        CTGCAGCTTTTGGTGATAAAATTTTCTATATTGGAGGGTTGCATATTGCTACCAATTCCG
NM_015483.2         CTGCAGCTTTTGGTGATAAAATTTTCTATATTGGAGGGTTGCATATTGCTACCAATTCCG
XM_005249696.1      CTGCAGCTTTTGGTGATAAAATTTTCTATATTGGAGGGTTGCATATTGCTACCAATTCCG
                    ************************************************************

HsCD00399349        GCATAAGACTCCCCTCTGGCACTGTAGATGGGTCTTCAGTAACTGTGGAAATTTATGATG
NM_015483.2         GCATAAGACTCCCCTCTGGCACTGTAGATGGGTCTTCAGTAACTGTGGAAATTTATGATG
XM_005249696.1      GCATAAGACTCCCCTCTGGCACTGTAGATGGGTCTTCAGTAACTGTGGAAATTTATGATG
                    ************************************************************

HsCD00399349        TGAATAAAAATGAGTGGAAAATGGCAGCCAACATCCCTGCTAAGAGGTACTCTGACCCCT
NM_015483.2         TGAATAAAAATGAGTGGAAAATGGCAGCCAACATCCCTGCTAAGAGGTACTCTGACCCCT
XM_005249696.1      TGAATAAAAATGAGTGGAAAATGGCAGCCAACATCCCTGCTAAGAGGTACTCTGACCCCT
                    ************************************************************

HsCD00399349        GTGTTAGAGCTGTTGTGATCTCAAATTCTATATGTGTGTTTATGCGAGAAACCCACTTAA
NM_015483.2         GTGTTAGAGCTGTTGTGATCTCAAATTCTCTATGTGTGTTTATGCGAGAAACCCACTTAA
XM_005249696.1      GTGTTAGAGCTGTTGTGATCTCAAATTCTCTATGTGTGTTTATGCGAGAAACCCACTTAA
                    *****************************.******************************

HsCD00399349        ATGAGCGAGCTAAATACGTCACCTACCAATATGACCTGGAACTTGACCGGTGGTCTCTGC
NM_015483.2         ATGAGCGAGCTAAATACGTCACCTACCAATATGACCTGGAACTTGACCGGTGGTCTCTGC
XM_005249696.1      ATGAGCGAGCTAAATACGTCACCTACCAATATGACCTGGAACTTGACCGGTGGTCTCTGC
                    ************************************************************

HsCD00399349        GGCAGCATATATCTGAACGTGTACTGTGGGACTTGGGGAGAGATTTTCGATGCACTGTGG
NM_015483.2         GGCAGCATATATCTGAACGTGTACTGTGGGACTTGGGGAGAGATTTTCGATGCACTGTGG
XM_005249696.1      GGCAGCATATATCTGAACGTGTACTGTGGGACTTGGGGAGAGATTTTCGATGCACTGTGG
                    ************************************************************

HsCD00399349        GGAAACTCTATCCATCCTGCCTTGAAGAGTCTCCATGGAAACCACCAACTTATCTTTTTT
NM_015483.2         GGAAACTCTATCCATCCTGCCTTGAAGAGTCTCCATGGAAACCACCAACTTATCTTTTTT
XM_005249696.1      GGAAACTCTATCCATCCTGCCTTGAAGAGTCTCCATGGAAACCACCAACTTATCTTTTTT
                    ************************************************************

HsCD00399349        CAACGGATGGGACAGAAGAGTTTGAACTGGATGGAGAAATGGTTGCACTACCACCTGTAT
NM_015483.2         CAACGGATGGGACAGAAGAGTTTGAACTGGATGGAGAAATGGTTGCACTACCACCTGTAT
XM_005249696.1      CAACGGATGGGACAGAAGAGTTTGAACTGGATGGAGAAATGGTTGCACTACCACCTGTAT
                    ************************************************************

HsCD00399349        ACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_015483.2         AG-------------------------------------------------
XM_005249696.1      AG-------------------------------------------------
                    *                                                  

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
HPRD : 13756

Clone

Cloning Information : 573447

Organism


TAX_ID : 9606
Species Specific ID: 25948

Genome

Chromosome : 7
Map Location : 7p14.3
Ensembl : ENSG00000170852

Reagents

Labome : KBTBD2-antibody