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NID1 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  NID1
Gene Name:  nidogen 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399373

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 3342nts         Open reading frame : 13 to 3354

CAGTTCGGGAACATGTTGGCCTCGAGCAGCCGGATCCGGGCTGCGTGGACGCGGGCGCTG
CTGCTGCCGCTGCTGCTGGCGGGGCCTGTGGGCTGCCTGAGCCGCCAGGAGCTCTTTCCC
TTCGGCCCCGGACAGGGGGACCTGGAGCTGGAGGACGGGGATGACTTCGTCTCTCCTGCC
CTGGAGCTGCGTGGGGCGCTCCGCTTCTACGACAGATCCGACATCGACGCAGTCTACGTC
ACCACAAATGGCATCATTGCTACGAGTGAACCCCCGGCCAAAGAATCCCATCCCGGGCTC
TTCCCACCAACATTCGGTGCAGTCGCCCCTTTCCTGGCGGACTTGGACACGACCGATGGC
CTGGGGAAGGTTTATTATCGAGAAGACTTATCCCCCTCCATCACTCAGCGAGCAGCAGAG
TGTGTCCACAGAGGGTTCCCGGAGATCTCTTTCCAGCCTAGTAGCGCGGTGGTTGTCACT
TGGGAATCCGTGGCCCCCTACCAAGGGCCCAGCAGGGACCCAGACCAGAAAGGCAAGAGA
AACACGTTCCAGGCTGTTCTAGCCTCCTCTGATTCCAGCTCCTATGCCATTTTCCTTTAT
CCTGAGGATGGTCTGCAGTTCCATACGACATTCTCAAAGAAGGAAAACAACCAAGTTCCT
GCCGTGGTTGCATTCAGTCAAGGTTCAGTGGGATTCTTATGGAAGAGCAACGGAGCTTAT
AACATATTTGCTAATGACAGGGAATCAATTGAAAATTTGGCCAAGAGTAGTAACTCTGGG
CAGCAGGGTGTCTGGGTGTTTGAGATTGGGAGTCCAGCCACCACCAATGGCGTGGTGCCT
GCAGACGTGATCCTCGGAACTGAAGATGGGGCAGAGTATGATGATGAGGATGAAGATTAT
GACCTGGCGACCACTCGTCTGGGCCTGGAGGATGTGGGCACCACGCCCTTCTCCTACAAG
GCTCTGAGAAGGGGAGGTGCTGACACATACAGTGTGCCCAGCGTCCTCTCCCCGCGCCGG
GCAGCTACCGAAAGGCCCCTTGGACCTCCCACAGAGAGAACCAGGTCTTTCCAGTTGGCA
GTGGAGACTTTTCACCAGCAGCACCCTCAGGTCATAGATGTGGATGAAGTTGAGGAAACA
GGAGTTGTTTTCAGCTATAACACGGATTCCCGCCAGACGTGTGCTAACAACAGACACCAG
TGCTCGGTGCACGCAGAGTGCAGGGACTACGCCACGGGCTTCTGCTGCAGCTGTGTCGCT
GGCTATACGGGCAATGGCAGGCAATGTGTTGCAGAAGGTTCCCCCCAGCGAGTCAATGGC
AAGGTGAAAGGAAGGATCTTTGTGGGGAGCAGCCAGGTCCCCATTGTCTTTGAGAACACT
GACCTCCACTCTTACGTAGTAATGAACCACGGGCGCTCCTACACAGCCATCAGCACCATT
CCCGAGACCGTTGGATATTCTCTGCTTCCACTGGCCCCAGTTGGAGGCATCATTGGATGG
ATGTTTGCAGTGGAGCAGGACGGATTCAAGAATGGGTTCAGCATCACCGGGGGTGAGTTC
ACTCGCCAGGCTGAGGTGACCTTCGTGGGGCACCCGGGCAATCTGGTCATTAAGCAGCGG
TTCAGCGGCATCGATGAGCATGGGCACCTGACCATCGACACGGAGCTGGAGGGCCGCGTG
CCGCAGATTCCGTTCGGCTCCTCCGTGCACATTGAGCCCTACACGGAGCTGTACCACTAC
TCCACCTCAGTGATCACTTCCTCCTCCACCCGGGAGTACACGGTGACTGAGCCCGAGCGA
GATGGGGCATCTCCTTCACGCATCTACACTTACCAGTGGCGCCAGACCATCACCTTCCAG
GAATGCGTCCACGATGACTCCCGGCCAGCCCTGCCCAGCACCCAGCAGCTCTCGGTGGAC
AGCGTGTTCGTCCTGTACAACCAGGAGGAGAAGATCTTGCGCTATGCTCTCAGCAACTCC
ATTGGGCCTGTGAGGGAAGGCTCCCCTGATGCTCTTCAGAATCCCTGCTACATCGGCACT
CATGGGTGTGACACCAACGCGGCCTGTCGCCCTGGTCCCAGGACACAGTTCACCTGCGAG
TGCTCCATCGGCTTCCGAGGAGACGGGCGAACCTGCTATGAGGTGGAGAAAACCCGGTGC
CAGCACGAGCGAGAACACATTCTCGGGGCGGCGGGGGCGACAGACCCACAGCGACCCATT
CCTCCGGGGCTGTTCGTTCCTGAGTGCGATGCGCACGGGCACTACGCGCCCACCCAGTGC
CACGGCAGCACCGGCTACTGCTGGTGCGTGGATCGCGACGGCCGCGAGGTGGAGGGCACC
AGGACCAGGCCCGGGATGACGCCCCCGTGTCTGAGTACAGTGGCTCCCCCGATTCACCAA
GGACCTGCGGTGCCTACCGCCGTGATCCCCTTGCCTCCTGGGACCCATTTACTCTTTGCC
CAGACTGGGAAGATTGAGCGCCTGCCCCTGGAGGGAAATACCATGAGGAAGACAGAAGCA
AAGGCGTTCCTTCATGTCCCGGCTAAAGTCATCATTGGACTGGCCTTTGACTGCGTGGAC
AAGATGGTTTACTGGACGGACATCACTGAGCCTTCCATTGGGAGAGCTAGTCTACATGGT
GGAGAGCCAACCACCATCATTAGACAAGATCTTGGAAGTCCAGAAGGTATCGCTGTTGAT
CACCTTGGCCGCAACATCTTCTGGACAGACTCTAACCTGGATCGAATAGAAGTGGCGAAG
CTGGACGGCACGCAGCGCCGGGTGCTCTTTGAGACTGACTTGGTGAATCCCAGAGGCATT
GTAACGGATTCCGTGAGAGGGAACCTTTACTGGACAGACTGGAACAGAGATAACCCCAAG
ATTGAAACTTCCTACATGGACGGCACGAACCGGAGGATCCTTGTGCAGGATGACCTGGGC
TTGCCCAATGGACTGACCTTCGATGCGTTCTCATCTCAGCTCTGCTGGGTGGATGCAGGC
ACCAATCGGGCGGAATGCCTGAACCCCAGTCAGCCCAGCAGACGCAAGGCTCTCGAAGGG
CTCCAGTATCCTTTTGCTGTGACGAGCTACGGGAAGAATCTGTATTTCACAGACTGGAAG
ATGAATTCCGTGGTTGCTCTCGATCTTGCAATTTCCAAGGAGACGGATGCTTTCCAACCC
CACAAGCAGACCCGGCTGTATGGCATCACCACGGCCCTGTCTCAGTGTCCGCAAGGCCAT
AACTACTGCTCAGTGAACAATGGCGGCTGCACCCACCTATGCTTGGCCACCCCAGGGAGC
AGGACCTGCCGTTGCCCTGACAACACCTTGGGAGTTGACTGTATCGAACAGAAATGAAGA
CAAGAGTGCCTTATTTCCTTTCCAAGTATTTCACAGCAACACTCTACTTGAAGCAACTTG
GTCCAGATTGAAAAGTGTCCTCTGGCTGAGTGGCCACTAGGCCCAGACCCAGCCCAGCCT
GAGCCCCAACAACTTTTCCCTCACTGTTCCCCAAAACATGCACCCTGGACTTCTCTAATA
GAAAAGTCTCCACCCCTACACAAGGACAGAACCCTCCACCCCTACCCCCAACCCTCAGAC
AGACTTATACACCCCTGAGTGAGGATTACATGCCCATCCCAGTGTCCTAGGACCTTTTCC
CAATACTAGCCCCCCAGTGGTGAACAGAACCTCCCAAATTTGAGTTGCACCCTTCCCTGT
GGCCTTATGAGCTCAGCCTCGCTTTGAGGTACCCACCGTCCTGTCAGCTCCTTGACCTAT
GAGCCGGGGCCTGACTAGGAAAAGTTGGGAGTTAAGGAGGAAATTAGCATTCCTTAATGT
TTTGTTTTGGTGCTCTGAATTTCTTCTTTATTATAGTCCTATAGTTTTACTCCTCAGTTC
CTCACCATCATCATCTTGTCTAAGACCCCCATTATAATATTCATGCGCTGCTTTTTCATC
AAAACCTACCCTGTCCTAGAGATCTATGGGCATTTGGTGGATGATAATGAGCAGCCCCTC
CCAGATAGAATGTCAATATTTGAGCAGTAGGATATTGGCATTTGTTAGTTAAAGGCTTAA
ATCAAAAGAATGTCCAATGGTAGGAATTTCAAGGTGTAGGTCAGATATTTGAGAATAGGG
GATTTTTTTGATGTGCCTTAAATTATACCAAAGATTACTAATTATTCCTCTTTGCCCAAA
ATACTTGCATCCAAGGTTCTAGTCTCTGTTGCTGTGCTGGTCTTTAGCCCCACTGCTTGC
ACTGATGTCCCTCCTTTTCACGGAGACCTATCTGAGGTACAGGATGGGGCTGGCACCAGA
TGATGTCCCACCACAGTCCCTCACCTCCGGCCTCCACATGACAGAACCAATTTACACTCA
ACCATGACCTCACCCCTCCTTGGTTTCTCCCTCGATCTGTGGCCCTTTTTGGATGTATTC
TTATCTAACAACACAATCCGGAAAGACTGAATTGAATATTTATACTAATGGTTCATATCC
TTTATTGCTCAATGATCTAATTAAAGGGATCATTGCCACATTTCATGTTTATATTTCTAC
AATTTGTTTAGAAAACATCTCCTGACCATATCAGTAGCTCGTGTTATCTTTTTATCAACT
GCTTCCCAGAGTCCTAAAACAATAGAAATTTTGGATTGAAAAGTTCAGCATAAGGAGTTT
GAGTCAGTAAAGGATGGGATAAAGGAGTCGAGATGATTCAATGAAAAGTATCACAAAAAA
GAGATTGATCAACAAGAGAAATAAAAAAGCCCAAGAGGAAGTGGTAGGGGAAGGAATTTA
AGAACAGCAATAAGTAAAACTCTTAAGTAACTCCAAAAAGAAAATGGTACATTTTGCCAA
AGACCACTTATACTTGAGAACATGGAAGAATTTGCCTGATACTCTCTTTGGGGAAAAGAG
TCTCTCCTCTTTTCCTCAAACCCCAGTACACTCAGCCTCTCTGCCCCACCTTCTCCTGAC
TTTGTCCTCACTTGCTTCTGCAGTACATTGGAACCTGAATTGAAAGAAAGTCTTCCTTGA
ATAATTGGAGTTTGTCTTGAGAGGCAAATATAGCCCCAAGAATCACAAGATTCGAGGACC
ATGTAGGTCTTTTACGTAGCCCAAATCCATAAATTAGTCTCACTTTTTGTATTTATCGTT
TCATATTAAACCCTCTATATCAAATGTTCATCATGATTTTGTATGATTTTTATAACTATT
TTATTCATTTTATTAGATTTATTCTAAAATTTTTTAATGGTAAATTCTTAAACTGTGGAA
ACCACTGAAGGTGCTTATTAACTGTTCTCCCAGATTTGTACAAGTATTGGATGATTCCTT
GAGTTTACAGCTGTACAAATAGTGTGGAAAATAAACTTTTTTTAAAAAAGAAAAAAAAAA
AAAAAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : NID1
Symbol Nomenclature : NID1
SYNONYM : NID
Designation : NID-1|enactin|entactin|nidogen-1
Full Nomenclature : nidogen 1
GENEID : 4811
GenBank Accession : BC045606
HGNC : 7821
MIM : 131390
Vega : OTTHUMG00000040071
Target GenBank: BC045606

 

Pathways

NCI : Beta1 integrin cell surface interactions
Reactome : Degradation of the extracellular matrix
Reactome : Extracellular matrix organization
Reactome : Laminin interactions

Clone

Cloning Information : 573471

Organism


TAX_ID : 9606
Species Specific ID: 4811

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000116962

Reagents

Labome : NID1-antibody