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KLHL26 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL26
Gene Name:  kelch-like family member 26
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073736)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399754

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1845nts         Open reading frame : 51 to 1895
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCAGAGT
CCGGCGGTAGCAGCGGTGGTGCTGGTGGCGGCGGCGCTTTCGGCGCGGGCCCGGGCCCCG
AGCGCCCGAACAGCACGGCCGACAAGAACGGGGCCCTCAAGTGCACCTTCTCGGCACCCA
GCCACAGCACCAGCCTCCTGCAGGGCCTGGCCACCCTCCGCGCTCAGGGCCAGCTCCTCG
ATGTTGTGCTGACTATTAACAGAGAGGCCTTTCCTGCACACAAGGTCGTCCTGGCTGCCT
GCAGCGACTACTTCAGGGCCATGTTCACCGGCGGCATGCGGGAGGCAAGCCAGGACGTCA
TCGAGCTGAAGGGCGTGTCGGCCCGTGGCCTGCGGCACATCATCGACTTCGCCTACAGCG
CCGAGGTGACACTGGACCTGGACTGCGTGCAGGACGTGCTGGGCGCGGCCGTGTTCTTGC
AGATGCTGCCCGTGGTGGAGCTGTGCGAGGAGTTCCTGAAGGCGGCCATGAGCGTGGAGA
CCTGCCTCAACATCGGCCAGATGGCCACCACCTTCAGCCTGGCCTCGCTGCGAGAGTCGG
TGGATGCCTTCACCTTCCGGCACTTCCTGCAGATCGCCGAGGAGGAGGATTTCCTGCGCC
TGCCACTGGAGCGCCTGGTCTTCTTCCTGCAGAGCAACCGGCTGCAGAGCTGTGCCGAGA
TCGACCTGTTCCGCGCGGCCGTCCGCTGGCTGCAGCATGACCCGGCCCGGCGGCCGCGCG
CCAGCCACGTGCTCTGCCACATTCGCTTCCCGCTCATGCAGTCGTCCGAGCTGGTGGACA
GCGTGCAGACGCTGGACATCATGGTGGAGGACGTGCTGTGCCGCCAGTATCTGCTGGAGG
CCTTCAACTACCAGGTGCTGCCCTTCCGGCAGCACGAGATGCAGTCTCCGCGCACTGCCG
TGCGCTCGGATGTGCCCTCGCTCGTCACCTTCGGCGGCACGCCCTACACCGACAGCGACC
GCTCGGTCAGCAGCAAGGTCTACCAGCTGCCTGAGCCGGGAGCCCGCCACTTCCGCGAGC
TCACGGAGATGGAGGTAGGCTGCAGCCACACGTGCGTGGCCGTGCTGGACAATTTTGTGT
ACGTGGCCGGGGGGCAGCACCTGCAGTACCGCAGCGGCGAGGGCGCAGTGGACGCCTGCT
ACCGCTACGACCCCCACCTGAATCGCTGGCTGCGCCTGCAGGCCATGCAGGAAAGCCGCA
TCCAGTTCCAGCTGAACGTGCTGTGCGGCATGGTGTACGCCACGGGCGGCCGCAACCGAG
CCGGCAGCCTGGCCTCCGTGGAGCGGTACTGCCCCCGGCGCAATGAGTGGGGCTACGCCT
GCTCGCTGAAGCGCCGTACCTGGGGCCATGCTGGGGCCGCCTCAGGGGGCCGCCTCTACA
TCTCGGGTGGCTACGGGATCTCAGTGGAGGACAAGAAGGCCCTGCACTGCTACGACCCCG
TGGCCGACCAGTGGGAGTTCAAGGCGCCCATGAGCGAACCCCGCGTGCTACACGCCATGG
TGGGTGCCGGCGGCCGCATCTATGCCCTCGGGGGCCGCATGGACCACGTGGACCGCTGCT
TCGACGTGCTGGCTGTGGAGTACTATGTGCCGGAGACGGACCAGTGGACCAGCATGAGCC
CCATGCGGGCCGGCCAGTCAGAGGCCGGCTGCTGCCTGCTGGAGAGGAAGATCTACATCG
TCGGGGGCTACAACTGGCGTCTCAACAACGTCACGGGCATCGTACAGGTGTACAACACGG
ACACCGACGAGTGGGAGCGGGACCTGCACTTCCCGGAGTCCTTCGCAGGCATAGCCTGCG
CCCCCGTCCTGCTGCCCCGGGCCGGGACCAGGAGGTACCCAACTTTCTTGTACAAAGTTG
GCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL26
Symbol Nomenclature : KLHL26
Designation : kelch-like 26|kelch-like protein 26
Full Nomenclature : kelch-like family member 26
GENEID : 55295
GenBank Accession : JF432514
HGNC : 25623
Vega : OTTHUMG00000183114
Target GenBank: JF432514

 

Reference Sequence Alignment

  

HsCD00399754      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCAGAGT
NM_018316.1       --------------------------------------------------ATGGCGGAGT
                                                                    *****.****

HsCD00399754      CCGGCGGTAGCAGCGGTGGTGCTGGTGGCGGCGGCGCTTTCGGCGCGGGCCCGGGCCCCG
NM_018316.1       CCGGCGGTAGCAGCGGTGGTGCTGGTGGCGGCGGCGCTTTCGGCGCGGGCCCGGGCCCCG
                  ************************************************************

HsCD00399754      AGCGCCCGAACAGCACGGCCGACAAGAACGGGGCCCTCAAGTGCACCTTCTCGGCACCCA
NM_018316.1       AGCGCCCGAACAGCACGGCCGACAAGAACGGGGCCCTCAAGTGCACCTTCTCGGCACCCA
                  ************************************************************

HsCD00399754      GCCACAGCACCAGCCTCCTGCAGGGCCTGGCCACCCTCCGCGCTCAGGGCCAGCTCCTCG
NM_018316.1       GCCACAGCACCAGCCTCCTGCAGGGCCTGGCCACCCTCCGCGCTCAGGGCCAGCTCCTCG
                  ************************************************************

HsCD00399754      ATGTTGTGCTGACTATTAACAGAGAGGCCTTTCCTGCACACAAGGTCGTCCTGGCTGCCT
NM_018316.1       ATGTTGTGCTGACTATTAACAGAGAGGCCTTTCCTGCACACAAGGTCGTCCTGGCTGCCT
                  ************************************************************

HsCD00399754      GCAGCGACTACTTCAGGGCCATGTTCACCGGCGGCATGCGGGAGGCAAGCCAGGACGTCA
NM_018316.1       GCAGCGACTACTTCAGGGCCATGTTCACCGGCGGCATGCGGGAGGCAAGCCAGGACGTCA
                  ************************************************************

HsCD00399754      TCGAGCTGAAGGGCGTGTCGGCCCGTGGCCTGCGGCACATCATCGACTTCGCCTACAGCG
NM_018316.1       TCGAGCTGAAGGGCGTGTCGGCCCGTGGCCTGCGGCACATCATCGACTTCGCCTACAGCG
                  ************************************************************

HsCD00399754      CCGAGGTGACACTGGACCTGGACTGCGTGCAGGACGTGCTGGGCGCGGCCGTGTTCTTGC
NM_018316.1       CCGAGGTGACACTGGACCTGGACTGCGTGCAGGACGTGCTGGGCGCGGCCGTGTTCTTGC
                  ************************************************************

HsCD00399754      AGATGCTGCCCGTGGTGGAGCTGTGCGAGGAGTTCCTGAAGGCGGCCATGAGCGTGGAGA
NM_018316.1       AGATGCTGCCCGTGGTGGAGCTGTGCGAGGAGTTCCTGAAGGCGGCCATGAGCGTGGAGA
                  ************************************************************

HsCD00399754      CCTGCCTCAACATCGGCCAGATGGCCACCACCTTCAGCCTGGCCTCGCTGCGAGAGTCGG
NM_018316.1       CCTGCCTCAACATCGGCCAGATGGCCACCACCTTCAGCCTGGCCTCGCTGCGAGAGTCGG
                  ************************************************************

HsCD00399754      TGGATGCCTTCACCTTCCGGCACTTCCTGCAGATCGCCGAGGAGGAGGATTTCCTGCGCC
NM_018316.1       TGGATGCCTTCACCTTCCGGCACTTCCTGCAGATCGCCGAGGAGGAGGATTTCCTGCGCC
                  ************************************************************

HsCD00399754      TGCCACTGGAGCGCCTGGTCTTCTTCCTGCAGAGCAACCGGCTGCAGAGCTGTGCCGAGA
NM_018316.1       TGCCACTGGAGCGCCTGGTCTTCTTCCTGCAGAGCAACCGGCTGCAGAGCTGTGCCGAGA
                  ************************************************************

HsCD00399754      TCGACCTGTTCCGCGCGGCCGTCCGCTGGCTGCAGCATGACCCGGCCCGGCGGCCGCGCG
NM_018316.1       TCGACCTGTTCCGCGCGGCCGTCCGCTGGCTGCAGCATGACCCGGCCCGGCGGCCGCGCG
                  ************************************************************

HsCD00399754      CCAGCCACGTGCTCTGCCACATTCGCTTCCCGCTCATGCAGTCGTCCGAGCTGGTGGACA
NM_018316.1       CCAGCCACGTGCTCTGCCACATTCGCTTCCCGCTCATGCAGTCGTCCGAGCTGGTGGACA
                  ************************************************************

HsCD00399754      GCGTGCAGACGCTGGACATCATGGTGGAGGACGTGCTGTGCCGCCAGTATCTGCTGGAGG
NM_018316.1       GCGTGCAGACGCTGGACATCATGGTGGAGGACGTGCTGTGCCGCCAGTATCTGCTGGAGG
                  ************************************************************

HsCD00399754      CCTTCAACTACCAGGTGCTGCCCTTCCGGCAGCACGAGATGCAGTCTCCGCGCACTGCCG
NM_018316.1       CCTTCAACTACCAGGTGCTGCCCTTCCGGCAGCACGAGATGCAGTCTCCGCGCACCGCCG
                  ******************************************************* ****

HsCD00399754      TGCGCTCGGATGTGCCCTCGCTCGTCACCTTCGGCGGCACGCCCTACACCGACAGCGACC
NM_018316.1       TGCGCTCGGATGTGCCCTCGCTCGTCACCTTCGGCGGCACGCCCTACACCGACAGCGACC
                  ************************************************************

HsCD00399754      GCTCGGTCAGCAGCAAGGTCTACCAGCTGCCTGAGCCGGGAGCCCGCCACTTCCGCGAGC
NM_018316.1       GCTCGGTCAGCAGCAAGGTCTACCAGCTGCCTGAGCCGGGAGCCCGCCACTTCCGCGAGC
                  ************************************************************

HsCD00399754      TCACGGAGATGGAGGTAGGCTGCAGCCACACGTGCGTGGCCGTGCTGGACAATTTTGTGT
NM_018316.1       TCACGGAGATGGAGGTAGGCTGCAGCCACACGTGCGTGGCCGTGCTGGACAATTTTGTGT
                  ************************************************************

HsCD00399754      ACGTGGCCGGGGGGCAGCACCTGCAGTACCGCAGCGGCGAGGGCGCAGTGGACGCCTGCT
NM_018316.1       ACGTGGCCGGGGGGCAGCACCTGCAGTACCGCAGCGGCGAGGGCGCAGTGGACGCCTGCT
                  ************************************************************

HsCD00399754      ACCGCTACGACCCCCACCTGAATCGCTGGCTGCGCCTGCAGGCCATGCAGGAAAGCCGCA
NM_018316.1       ACCGCTACGACCCCCACCTGAATCGCTGGCTGCGCCTGCAGGCCATGCAGGAAAGCCGCA
                  ************************************************************

HsCD00399754      TCCAGTTCCAGCTGAACGTGCTGTGCGGCATGGTGTACGCCACGGGCGGCCGCAACCGAG
NM_018316.1       TCCAGTTCCAGCTGAACGTGCTGTGCGGCATGGTGTACGCCACGGGCGGCCGCAACCGAG
                  ************************************************************

HsCD00399754      CCGGCAGCCTGGCCTCCGTGGAGCGGTACTGCCCCCGGCGCAATGAGTGGGGCTACGCCT
NM_018316.1       CCGGCAGCCTGGCCTCCGTGGAGCGGTACTGCCCCCGGCGCAATGAGTGGGGCTACGCCT
                  ************************************************************

HsCD00399754      GCTCGCTGAAGCGCCGTACCTGGGGCCATGCTGGGGCCGCCTCAGGGGGCCGCCTCTACA
NM_018316.1       GCTCGCTGAAGCGCCGTACCTGGGGCCATGCTGGGGCCGCCTCAGGGGGCCGCCTCTACA
                  ************************************************************

HsCD00399754      TCTCGGGTGGCTACGGGATCTCAGTGGAGGACAAGAAGGCCCTGCACTGCTACGACCCCG
NM_018316.1       TCTCGGGTGGCTACGGGATCTCAGTGGAGGACAAGAAGGCCCTGCACTGCTACGACCCCG
                  ************************************************************

HsCD00399754      TGGCCGACCAGTGGGAGTTCAAGGCGCCCATGAGCGAACCCCGCGTGCTACACGCCATGG
NM_018316.1       TGGCCGACCAGTGGGAGTTCAAGGCGCCCATGAGCGAACCCCGCGTGCTACACGCCATGG
                  ************************************************************

HsCD00399754      TGGGTGCCGGCGGCCGCATCTATGCCCTCGGGGGCCGCATGGACCACGTGGACCGCTGCT
NM_018316.1       TGGGTGCCGGCGGCCGCATCTATGCCCTCGGGGGCCGCATGGACCACGTGGACCGCTGCT
                  ************************************************************

HsCD00399754      TCGACGTGCTGGCTGTGGAGTACTATGTGCCGGAGACGGACCAGTGGACCAGCATGAGCC
NM_018316.1       TCGACGTGCTGGCTGTGGAGTACTATGTGCCGGAGACGGACCAGTGGACCAGCGTGAGCC
                  *****************************************************.******

HsCD00399754      CCATGCGGGCCGGCCAGTCAGAGGCCGGCTGCTGCCTGCTGGAGAGGAAGATCTACATCG
NM_018316.1       CCATGCGGGCCGGCCAGTCAGAGGCCGGCTGCTGCCTGCTGGAGAGGAAGATCTACATCG
                  ************************************************************

HsCD00399754      TCGGGGGCTACAACTGGCGTCTCAACAACGTCACGGGCATCGTACAGGTGTACAACACGG
NM_018316.1       TCGGGGGCTACAACTGGCGTCTCAACAACGTCACGGGCATCGTACAGGTGTACAACACGG
                  ************************************************************

HsCD00399754      ACACCGACGAGTGGGAGCGGGACCTGCACTTCCCGGAGTCCTTCGCAGGCATAGCCTGCG
NM_018316.1       ACACCGACGAGTGGGAGCGGGACCTGCACTTCCCGGAGTCCTTCGCAGGCATAGCCTGCG
                  ************************************************************

HsCD00399754      CCCCCGTCCTGCTGCCCCGGGCCGGGACCAGGAGGTACCCAACTTTCTTGTACAAAGTTG
NM_018316.1       CCCCCGTCCTGCTGCCCCGGGCCGGGACCAGGAGGTAG----------------------
                  *************************************                       

HsCD00399754      GCATTATAAGAAAGCATTGCTTATCAA
NM_018316.1       ---------------------------
                                             

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
HPRD : 07728

Clone

Cloning Information : 573852

Organism


TAX_ID : 9606
Species Specific ID: 55295

Genome

Chromosome : 19
Map Location : 19p13.11
Ensembl : ENSG00000167487

Reagents

Labome : KLHL26-antibody