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ACTL8 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTL8
Gene Name:  actin-like 8
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100073931)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00399970

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1098nts        
Open reading frame : 51 to 1148
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCTTCAA
GAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCCGGATGGAATG
AGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAGAACCCTGGCC
CCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCTGACACCTTTA
GCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTACCTCTGGTCAT
TTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATCACGGAGACAC
CCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAGTTGCTGCATG
TCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCTGGCCTCCTGA
CCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTCCACCAGGGCC
GCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTCTCCGCCTATC
TCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAGCTGGAGACAG
TCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAGGCCCTGGACT
TTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTCCCAGACGGCT
CCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTTAGCCCGCAGG
TGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTGGAGTCCTGCG
AGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGCAACACCCTCT
ATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTCTCCTCCACCA
AGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGAGCGTCCGTGG
TGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTATGGTGAGCATA
TGAGGATGTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTL8
Symbol Nomenclature : ACTL8
SYNONYM : CT57
Designation : actin like protein|actin-like protein 8|cancer/testis antigen 57
Full Nomenclature : actin-like 8
GENEID : 81569
GenBank Accession : JF432692
HGNC : 24018
Vega : OTTHUMG00000002512
Target GenBank: JF432692

 

Reference Sequence Alignment

  

HsCD00399970      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGCTTCAA
NM_030812.2       --------------------------------------------------ATGGCTGCAA
                                                                    ****** ***

HsCD00399970      GAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCCGGATGGAATG
NM_030812.2       GAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCCGGCTGGAATG
                  ****************************************************.*******

HsCD00399970      AGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAGAACCCTGGCC
NM_030812.2       AGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAGAACCCTGGCC
                  ************************************************************

HsCD00399970      CCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCTGACACCTTTA
NM_030812.2       CCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCTGACACCTTTA
                  ************************************************************

HsCD00399970      GCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTACCTCTGGTCAT
NM_030812.2       GCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTACCTCTGGTCAT
                  ************************************************************

HsCD00399970      TTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATCACGGAGACAC
NM_030812.2       TTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATCACGGAGACAC
                  ************************************************************

HsCD00399970      CCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAGTTGCTGCATG
NM_030812.2       CCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAGTTGCTGCATG
                  ************************************************************

HsCD00399970      TCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCTGGCCTCCTGA
NM_030812.2       TCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCTGGCCTCCTGA
                  ************************************************************

HsCD00399970      CCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTCCACCAGGGCC
NM_030812.2       CCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTCCACCAGGGCC
                  ************************************************************

HsCD00399970      GCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTCTCCGCCTATC
NM_030812.2       GCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTCTCCGCCTATC
                  ************************************************************

HsCD00399970      TCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAGCTGGAGACAG
NM_030812.2       TCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAGCTGGAGACAG
                  ************************************************************

HsCD00399970      TCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAGGCCCTGGACT
NM_030812.2       TCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAGGCCCTGGACT
                  ************************************************************

HsCD00399970      TTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTCCCAGACGGCT
NM_030812.2       TTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTCCCAGACGGCT
                  ************************************************************

HsCD00399970      CCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTTAGCCCGCAGG
NM_030812.2       CCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTTAGCCCGCAGG
                  ************************************************************

HsCD00399970      TGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTGGAGTCCTGCG
NM_030812.2       TGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTGGAGTCCTGCG
                  ************************************************************

HsCD00399970      AGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGCAACACCCTCT
NM_030812.2       AGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGCAACACCCTCT
                  ************************************************************

HsCD00399970      ATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTCTCCTCCACCA
NM_030812.2       ATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTCTCCTCCACCA
                  ************************************************************

HsCD00399970      AGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGAGCGTCCGTGG
NM_030812.2       AGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGAGCGTCCGTGG
                  ************************************************************

HsCD00399970      TGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTATGGTGAGCATA
NM_030812.2       TGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTATGGTGAGCATA
                  ************************************************************

HsCD00399970      TGAGGATGTGCCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_030812.2       TGAGGATGTGA-------------------------------------------------
                  **********.                                                 

Protein

SMART domain : ACTIN : Actin
HPRD : 11274

Clone

Cloning Information : 574068

Organism


TAX_ID : 9606
Species Specific ID: 81569

Genome

Chromosome : 1
Map Location : 1p36.2-p35
Ensembl : ENSG00000117148

Reagents

Labome : ACTL8-antibody