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CAPN7 (Homo sapiens) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN7
Gene Name:  calpain 7
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: None
Comments: The ORFeome Collaboration entry clone (IMAGE:100074151)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
HsCD00400039

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2439nts         Open reading frame : 51 to 2489
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGACGCCA
CAGCACTGGAACGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCGC
TAGACAGAGCAGAAGCGCTGGGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
CAAGTGTTAAGCCAAAGCCACCTCCAGAGAGAGCACATTTTCCACTGGGCGCTAATCCCT
TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
GTTGGAAAGGAAGATACAGTGAAAATGATGTAGAAAACTGGACCCCAGAGTTGCAAAAGT
ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
TTATCCCCATCAAGATCACACAACTTCAGTGCCCAACTTTCTTGTACAAAGTTGGCATTA
TAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN7
Symbol Nomenclature : CAPN7
SYNONYM : CALPAIN7; PALBH
Designation : calpain like protease|calpain-7|homolog of Aspergillus Nidulans PALB|palB homolog
Full Nomenclature : calpain 7
GENEID : 23473
GenBank Accession : JF432832
HGNC : 1484
MIM : 606400
Vega : OTTHUMG00000129863
Target GenBank: JF432832

 

Reference Sequence Alignment

  

HsCD00400039      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGACGCCA
NM_014296.2       --------------------------------------------------ATGGACGCCA
                                                                    **********

HsCD00400039      CAGCACTGGAACGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
NM_014296.2       CAGCACTGGAGCGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
                  **********.*************************************************

HsCD00400039      GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
NM_014296.2       GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
                  ************************************************************

HsCD00400039      AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
NM_014296.2       AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
                  ************************************************************

HsCD00400039      TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
NM_014296.2       TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
                  ************************************************************

HsCD00400039      AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
NM_014296.2       AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
                  ************************************************************

HsCD00400039      AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
NM_014296.2       AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
                  ************************************************************

HsCD00400039      CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCGC
NM_014296.2       CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCAC
                  **********************************************************.*

HsCD00400039      TAGACAGAGCAGAAGCGCTGGGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
NM_014296.2       TAGACAGAGCAGAAGCGCTGAGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
                  ********************.***************************************

HsCD00400039      CAAGTGTTAAGCCAAAGCCACCTCCAGAGAGAGCACATTTTCCACTGGGCGCTAATCCCT
NM_014296.2       CAAGTGTTAAGCCAAAGCCACCTCCAGTGAGAGCACATTTTCCACTGGGCGCTAATCCCT
                  ***************************:********************************

HsCD00400039      TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
NM_014296.2       TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
                  ************************************************************

HsCD00400039      ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
NM_014296.2       ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
                  ************************************************************

HsCD00400039      TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
NM_014296.2       TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
                  ************************************************************

HsCD00400039      GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
NM_014296.2       GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
                  ************************************************************

HsCD00400039      CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
NM_014296.2       CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
                  ************************************************************

HsCD00400039      AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
NM_014296.2       AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
                  ************************************************************

HsCD00400039      GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
NM_014296.2       GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
                  ************************************************************

HsCD00400039      CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
NM_014296.2       CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
                  ************************************************************

HsCD00400039      AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
NM_014296.2       AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
                  ************************************************************

HsCD00400039      CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
NM_014296.2       CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
                  ************************************************************

HsCD00400039      GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
NM_014296.2       GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
                  ************************************************************

HsCD00400039      TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
NM_014296.2       TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
                  ************************************************************

HsCD00400039      GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
NM_014296.2       GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
                  ************************************************************

HsCD00400039      TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
NM_014296.2       TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
                  ************************************************************

HsCD00400039      ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
NM_014296.2       ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
                  ************************************************************

HsCD00400039      GTTGGAAAGGAAGATACAGTGAAAATGATGTAGAAAACTGGACCCCAGAGTTGCAAAAGT
NM_014296.2       GTTGGAAAGGAAGATACAGTGAAAATGATGTAAAAAACTGGACTCCAGAGTTGCAAAAGT
                  ********************************.********** ****************

HsCD00400039      ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
NM_014296.2       ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
                  ************************************************************

HsCD00400039      GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
NM_014296.2       GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
                  ************************************************************

HsCD00400039      AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
NM_014296.2       AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
                  ************************************************************

HsCD00400039      ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
NM_014296.2       ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
                  ************************************************************

HsCD00400039      CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
NM_014296.2       CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
                  ************************************************************

HsCD00400039      AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
NM_014296.2       AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
                  ************************************************************

HsCD00400039      CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
NM_014296.2       CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
                  ************************************************************

HsCD00400039      TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
NM_014296.2       TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
                  ************************************************************

HsCD00400039      CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
NM_014296.2       CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
                  ************************************************************

HsCD00400039      CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
NM_014296.2       CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
                  ************************************************************

HsCD00400039      GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
NM_014296.2       GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
                  ************************************************************

HsCD00400039      CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
NM_014296.2       CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
                  ************************************************************

HsCD00400039      TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
NM_014296.2       TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
                  ************************************************************

HsCD00400039      ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
NM_014296.2       ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
                  ************************************************************

HsCD00400039      TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
NM_014296.2       TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
                  ************************************************************

HsCD00400039      TTATCCCCATCAAGATCACACAACTTCAGTGCCCAACTTTCTTGTACAAAGTTGGCATTA
NM_014296.2       TTATCCCCATCAAGATCACACAACTTCAGTGA----------------------------
                  *******************************.                            

HsCD00400039      TAAGAAAGCATTGCTTATCAA
NM_014296.2       ---------------------
                                       

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 574399

Organism


TAX_ID : 9606
Species Specific ID: 23473

Genome

Chromosome : 3
Map Location : 3p24
Ensembl : ENSG00000131375

Reagents

Labome : CAPN7-antibody