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ZNF461 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZNF461
Gene Name:  zinc finger protein 461
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH169957.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040053

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1311nts         Open reading frame : 1 to 1311
Reference Sequence Alignment

ATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATTTTCAGCGCTATCTGGGAAGGC
AACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGTTATTTTAGACAACTTATGATT
AACCATGAAAACATGCCCATTTTTAGCCAACATACTTTACTCACTCAAGAATTTTATGAT
AGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATCTTCAGTTACCATTTATTTTTT
AGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAATGTAAAGAATGCACAGAAATT
GTTAATACACCATGCCTTTTTAAACAACAGACAATTCAAAATGGTGACAAATGCAATGAG
TGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAACTTAAACATCTAAGAATTCAT
AATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAGGCCTTTAATTATGGCTCAGAA
CTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCTTATGAATGTAAAGAATGTGGG
AAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAGAGACTTCATACTGGTGAAAAA
CCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGTGGCTTTCAACTTACTGAACAC
CTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAAGAATGTGGAAAGACTTTTAGG
CATCGCTCACATCTTACTATACATCAGAGAATTCATACTGGTGAGAAACCCTATGAATGT
CGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTCTCACACCATCAGAAAATTCAT
TCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAGGCTTTTTGTGATGGCTTACAA
CTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCCTATGAGTGTAAGGAATGTGGG
AAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAGAGAATTCATACTGGTGAGAAA
CCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTTCATTCACACCTTATTCAACAT
CAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAGGAATGTGGGAAGGCCTTTAGC
TATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCTGGAAAGAAACCTTATCAATGC
GGGAAGGCGTTTAATCATAGATTACAACTTAACTTACATCAGACTCTTCATACTGGCGAG
AAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGTCTAGCATCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1730
Start on reference sequence 420
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF461
Symbol Nomenclature : ZNF461
SYNONYM : GIOT-1; GIOT1
Designation : gonadotropin inducible transcription repressor 1|gonadotropin-inducible ovary transcription repressor 1
Full Nomenclature : zinc finger protein 461
GENEID : 92283
HGNC : 21629
MIM : 608640
Vega : OTTHUMG00000180832
Target GenBank: BC028631

 

Reference Sequence Alignment

  

HsCD00040053        ------------------------------------------------------------
NM_153257.2         ATGGCCCATGAGTTGGTGATGTTCAGAGATGTGGCTATAGATGTCTCTCAGGAGGAATGG
XM_005259402.1      ATGGCCCATGAGTTGGTGATGTTCAGAGATGTGGCTATAGATGTCTCTCAGGAGGAATGG
XM_005259403.1      --------------------------------------------------------ATGG
                                                                                

HsCD00040053        ------------------------------------------------------------
NM_153257.2         GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAGGTGATGTTGGAGAATTATAGC
XM_005259402.1      GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAGGTGATGTTGGAGAATTATAGC
XM_005259403.1      GAATGCCTGAACCCAGCGCAGAGGAATTTGTACAAGGAG---------------------
                                                                                

HsCD00040053        ------------------------------------------------------------
NM_153257.2         AACTTGGTGTCACTTGGACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
XM_005259402.1      AACTTGGTGTCACTTGGACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
XM_005259403.1      ----------------GACTTTCTGTTTCTAAGCCAGCCGTAATCTCCTCATTGGAGCAA
                                                                                

HsCD00040053        ------------------------------------------------------------
NM_153257.2         GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAGGTACATGG
XM_005259402.1      GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAG--------
XM_005259403.1      GGGAAGGAGCCCTGGATGGTTGTGAGGGAAGAGACAGGAAGATGGTGCCCAGGTACATGG
                                                                                

HsCD00040053        ------------------------------------------------------------
NM_153257.2         AAAACTTGGGGATTTCACAATAATTTCTTGGACAATAATGAGGCTACAGACATAAATGCA
XM_005259402.1      ------------------------------------------------------------
XM_005259403.1      AAAACTTGGGGATTTCACAATAATTTCTTGGACAATAATGAGGCTACAGACATAAATGCA
                                                                                

HsCD00040053        ------------------------------------------------------------
NM_153257.2         GACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
XM_005259402.1      -ACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
XM_005259403.1      GACTTGGCATCCAGAGATGAGCCCCAGAAGTTATCTCCAAAAAGGGATATTTATGAAACA
                                                                                

HsCD00040053        ---------------------ATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
NM_153257.2         GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
XM_005259402.1      GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
XM_005259403.1      GAATTATCCCAGTGGGTTAACATGGAAGAATTTAAAAGCCATAGTCCTGAGAGATCAATT
                                         ***************************************

HsCD00040053        TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
NM_153257.2         TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
XM_005259402.1      TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
XM_005259403.1      TTCAGCGCTATCTGGGAAGGCAACTGTCATTTTGAGCAACATCAGGGACAAGAGGAGGGT
                    ************************************************************

HsCD00040053        TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
NM_153257.2         TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
XM_005259402.1      TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
XM_005259403.1      TATTTTAGACAACTTATGATTAACCATGAAAACATGCCCATTTTTAGCCAACATACTTTA
                    ************************************************************

HsCD00040053        CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
NM_153257.2         CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
XM_005259402.1      CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
XM_005259403.1      CTCACTCAAGAATTTTATGATAGAGAGAAAATCTCTGAATGTAAAAAGTGTAGAAAAATC
                    ************************************************************

HsCD00040053        TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
NM_153257.2         TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
XM_005259402.1      TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
XM_005259403.1      TTCAGTTACCATTTATTTTTTAGTCACCACAAAAGAACTCATTCTAAAGAACTTTCTGAA
                    ************************************************************

HsCD00040053        TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
NM_153257.2         TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
XM_005259402.1      TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
XM_005259403.1      TGTAAAGAATGCACAGAAATTGTTAATACACCATGCCTTTTTAAACAACAGACAATTCAA
                    ************************************************************

HsCD00040053        AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
NM_153257.2         AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
XM_005259402.1      AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
XM_005259403.1      AATGGTGACAAATGCAATGAGTGTAAAGAATGTTGGAAGGCCTTTGTTCATTGCTCACAA
                    ************************************************************

HsCD00040053        CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
NM_153257.2         CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
XM_005259402.1      CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
XM_005259403.1      CTTAAACATCTAAGAATTCATAATGGTGAAAAACGCTATGAATGTAACGAATGTGGGAAG
                    ************************************************************

HsCD00040053        GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
NM_153257.2         GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
XM_005259402.1      GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
XM_005259403.1      GCCTTTAATTATGGCTCAGAACTTACTCTACATCAAAGAATTCACACTGGTGAGAAACCT
                    ************************************************************

HsCD00040053        TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
NM_153257.2         TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
XM_005259402.1      TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
XM_005259403.1      TATGAATGTAAAGAATGTGGGAAGGCCTTTAGACAGCGATCACAGCTTACTCAACATCAG
                    ************************************************************

HsCD00040053        AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
NM_153257.2         AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
XM_005259402.1      AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
XM_005259403.1      AGACTTCATACTGGTGAAAAACCCTATGAATGTAAGCAATGTGGGAAGGCTTTTATTCGT
                    ************************************************************

HsCD00040053        GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
NM_153257.2         GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
XM_005259402.1      GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
XM_005259403.1      GGCTTTCAACTTACTGAACACCTGCGACTCCATACTGGAGAGAAACCTTATGAATGTAAA
                    ************************************************************

HsCD00040053        GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
NM_153257.2         GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
XM_005259402.1      GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
XM_005259403.1      GAATGTGGAAAGACTTTTAGGCATCGCTCACATCTTACTATACATCAGAGAATTCATACT
                    ************************************************************

HsCD00040053        GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
NM_153257.2         GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
XM_005259402.1      GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
XM_005259403.1      GGTGAGAAACCCTATGAATGTCGGGAATGTGGGAAGGCCTTTAGCTATCACTCAAGCTTC
                    ************************************************************

HsCD00040053        TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
NM_153257.2         TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
XM_005259402.1      TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
XM_005259403.1      TCACACCATCAGAAAATTCATTCTGGCAAGAAACCTTATGAATGTCATGAATGTGGGAAG
                    ************************************************************

HsCD00040053        GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
NM_153257.2         GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
XM_005259402.1      GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
XM_005259403.1      GCTTTTTGTGATGGCTTACAACTAACCCTACATCAGAGGATTCATACTGGTGAGAAACCC
                    ************************************************************

HsCD00040053        TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
NM_153257.2         TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
XM_005259402.1      TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
XM_005259403.1      TATGAGTGTAAGGAATGTGGGAAGACTTTTAGACAGTGTTCACACCTCAAAAGACATCAG
                    ************************************************************

HsCD00040053        AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
NM_153257.2         AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
XM_005259402.1      AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
XM_005259403.1      AGAATTCATACTGGTGAGAAACCTCATGAATGCATGATATGTGGTAAGGCCTTTAGACTT
                    ************************************************************

HsCD00040053        CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
NM_153257.2         CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
XM_005259402.1      CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
XM_005259403.1      CATTCACACCTTATTCAACATCAAAGAATTCATACTGGTGAGAAACCCTATGAATGTAAG
                    ************************************************************

HsCD00040053        GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
NM_153257.2         GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
XM_005259402.1      GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
XM_005259403.1      GAATGTGGGAAGGCCTTTAGCTATCATTCAAGCTTCTCACACCATCAGAGAATTCATTCT
                    ************************************************************

HsCD00040053        GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
NM_153257.2         GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
XM_005259402.1      GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
XM_005259403.1      GGAAAGAAACCTTATCAATGCGGGAAGGCGTTTAATCATAGATTACAACTTAACTTACAT
                    ************************************************************

HsCD00040053        CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
NM_153257.2         CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
XM_005259402.1      CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
XM_005259403.1      CAGACTCTTCATACTGGCGAGAAGCCAGTCAGGTTTCCTCTCCTCCCTCCCCATCCTAGT
                    ************************************************************

HsCD00040053        CTAGCATCATTG
NM_153257.2         CTAGCATCATGA
XM_005259402.1      CTAGCATCATGA
XM_005259403.1      CTAGCATCATGA
                    ********** .

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q8TAF7
HPRD : 16358

Clone

Cloning Information : 169957
HIP Master Clone ID : 143423
Original Clone ID : FLH169957.01L

Organism


TAX_ID : 9606
Species Specific ID: 92283

Genome

Chromosome : 19
Map Location : 19q13.12
Ensembl : ENSG00000197808

Reagents

Labome : ZNF461-antibody