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GTF2H1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2H1
Gene Name:  general transcription factor IIH, polypeptide 1, 62kDa
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH176643.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040270

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1647nts         Open reading frame : 1 to 1647
Reference Sequence Alignment

ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAG
GATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGA
TTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAA
GCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTCCATTTTTCC
AATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAGCAGCTGCTG
CCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATGCTGCAAGAA
GATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG
GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCAT
AAGCAGGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGC
TGTAACGGTCTAAGATATAATTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTAT
CCAGCAGTAAAAATGAAATATGCAGAAAATGTTCCCCACAACATGACAGAGAAGGAATTC
TGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATCGGCTGAATACAGGGTCAAAG
GATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAATGGTTTCATTA
GGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC
TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAAT
GCTGCCATCATCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGA
AAACAAGAAGCACAAAATGAACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGA
GATGCAGACTGCTTTCAGCCAGCAGTCAAAAGGGCGAAATTACAAGAGTCCATTGAATAT
GAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCACTAAACCTCAAGAAGTCAGAT
AGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAGTCAGGACATT
ATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG
GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTT
ATGCAGGGAGGAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAA
TTGAAACACTTATATGTAGCTGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCT
GTTAATACGCCATTCCTAGAAGAAAAGGTAGTGAAAATGAAAAGTAATTTGGAACGATTC
CAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGAGACAGTATTTAAGCACAAAT
TTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCACACATGGCAG
TCACGGCGTCTGATGAAGAAAACGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1816
Start on reference sequence 170
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
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  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2H1
Symbol Nomenclature : GTF2H1
SYNONYM : BTF2; P62; TFB1; TFIIH
Designation : BTF2 p62|TFIIH basal transcription factor complex p62 subunit|basic transcription factor 2 62 kDa subunit|general transcription factor IIH polypeptide 1|general transcription factor IIH subunit 1
Full Nomenclature : general transcription factor IIH, polypeptide 1, 62kDa
GENEID : 2965
HGNC : 4655
MIM : 189972
Vega : OTTHUMG00000167690
Target GenBank: BC000365

 

Reference Sequence Alignment

  

HsCD00040270        ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAG
NM_005316.3         ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAG
NM_001142307.1      ATGGCAACCTCATCTGAAGAAGTTTTGCTGATTGTAAAGAAAGTGCGTCAAAAGAAGCAG
                    ************************************************************

HsCD00040270        GATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGA
NM_005316.3         GATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGA
NM_001142307.1      GATGGAGCTCTGTACCTCATGGCAGAAAGAATTGCTTGGGCACCTGAAGGCAAAGATAGA
                    ************************************************************

HsCD00040270        TTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAA
NM_005316.3         TTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAA
NM_001142307.1      TTTACAATCAGCCATATGTATGCAGATATTAAATGCCAGAAAATTAGTCCAGAAGGAAAA
                    ************************************************************

HsCD00040270        GCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTCCATTTTTCC
NM_005316.3         GCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTCCATTTTTCC
NM_001142307.1      GCTAAAATTCAGCTTCAGCTGGTCCTACATGCAGGGGACACAACTAACTTCCATTTTTCC
                    ************************************************************

HsCD00040270        AATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAGCAGCTGCTG
NM_005316.3         AATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAGCAGCTGCTG
NM_001142307.1      AATGAAAGCACAGCAGTGAAAGAGCGAGATGCAGTAAAAGACCTTCTTCAGCAGCTGCTG
                    ************************************************************

HsCD00040270        CCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATGCTGCAAGAA
NM_005316.3         CCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATGCTGCAAGAA
NM_001142307.1      CCCAAATTCAAGAGGAAAGCAAATAAAGAACTGGAAGAGAAGAACAGAATGCTGCAAGAA
                    ************************************************************

HsCD00040270        GATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG
NM_005316.3         GATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG
NM_001142307.1      GATCCTGTTTTGTTTCAGCTTTATAAAGACCTTGTTGTGAGTCAAGTGATCAGTGCTGAG
                    ************************************************************

HsCD00040270        GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCAT
NM_005316.3         GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCAT
NM_001142307.1      GAATTCTGGGCCAATCGTTTAAATGTGAATGCAACAGATAGTTCTTCCACATCCAATCAT
                    ************************************************************

HsCD00040270        AAGCAGGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGC
NM_005316.3         AAGCAGGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGC
NM_001142307.1      AAGCAGGATGTTGGCATTTCTGCTGCATTTCTGGCTGATGTCCGGCCCCAAACTGATGGC
                    ************************************************************

HsCD00040270        TGTAACGGTCTAAGATATAATTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTAT
NM_005316.3         TGTAACGGTCTAAGATATAATTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTAT
NM_001142307.1      TGTAACGGTCTAAGATATAATTTAACTTCTGATATCATTGAGTCCATATTTAGGACCTAT
                    ************************************************************

HsCD00040270        CCAGCAGTAAAAATGAAATATGCAGAAAATGTTCCCCACAACATGACAGAGAAGGAATTC
NM_005316.3         CCAGCAGTAAAAATGAAATATGCAGAAAATGTTCCCCACAACATGACAGAGAAGGAATTC
NM_001142307.1      CCAGCAGTAAAAATGAAATATGCAGAAAATGTTCCCCACAACATGACAGAGAAGGAATTC
                    ************************************************************

HsCD00040270        TGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATCGGCTGAATACAGGGTCAAAG
NM_005316.3         TGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATCGGCTGAATACAGGGTCAAAG
NM_001142307.1      TGGACACGTTTTTTCCAGTCCCATTATTTTCACAGGGATCGGCTGAATACAGGGTCAAAG
                    ************************************************************

HsCD00040270        GATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAATGGTTTCATTA
NM_005316.3         GATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAATGGTTTCATTA
NM_001142307.1      GATCTCTTTGCAGAATGTGCCAAAATAGATGAAAAAGGCCTAAAAACAATGGTTTCATTA
                    ************************************************************

HsCD00040270        GGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC
NM_005316.3         GGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC
NM_001142307.1      GGAGTGAAAAACCCACTACTAGATTTAACAGCTTTGGAAGATAAACCATTAGATGAGGGC
                    ************************************************************

HsCD00040270        TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAAT
NM_005316.3         TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAAT
NM_001142307.1      TATGGCATTTCCTCTGTGCCATCTGCTTCCAATTCTAAATCCATAAAAGAGAATAGTAAT
                    ************************************************************

HsCD00040270        GCTGCCATCATCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGA
NM_005316.3         GCTGCCATCATCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGA
NM_001142307.1      GCTGCCATCATCAAGAGATTTAACCATCACAGTGCCATGGTCCTGGCAGCTGGACTCAGA
                    ************************************************************

HsCD00040270        AAACAAGAAGCACAAAATGAACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGA
NM_005316.3         AAACAAGAAGCACAAAATGAACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGA
NM_001142307.1      AAACAAGAAGCACAAAATGAACAAACTAGTGAGCCCAGCAACATGGATGGAAATTCCGGA
                    ************************************************************

HsCD00040270        GATGCAGACTGCTTTCAGCCAGCAGTCAAAAGGGCGAAATTACAAGAGTCCATTGAATAT
NM_005316.3         GATGCAGACTGCTTTCAGCCAGCAGTCAAAAGGGCGAAATTACAAGAGTCCATTGAATAT
NM_001142307.1      GATGCAGACTGCTTTCAGCCAGCAGTCAAAAGGGCGAAATTACAAGAGTCCATTGAATAT
                    ************************************************************

HsCD00040270        GAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCACTAAACCTCAAGAAGTCAGAT
NM_005316.3         GAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCACTAAACCTCAAGAAGTCAGAT
NM_001142307.1      GAAGACTTGGGGAAAAATAATTCTGTAAAAACGATTGCACTAAACCTCAAGAAGTCAGAT
                    ************************************************************

HsCD00040270        AGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAGTCAGGACATT
NM_005316.3         AGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAGTCAGGACATT
NM_001142307.1      AGGTATTATCATGGTCCAACTCCAATCCAGTCACTACAGTATGCAACAAGTCAGGACATT
                    ************************************************************

HsCD00040270        ATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG
NM_005316.3         ATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG
NM_001142307.1      ATTAATTCTTTTCAAAGTATTAGACAAGAAATGGAAGCTTATACACCCAAGTTAACTCAG
                    ************************************************************

HsCD00040270        GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTT
NM_005316.3         GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTT
NM_001142307.1      GTTCTCTCAAGTAGTGCTGCCAGTAGTACCATCACAGCACTGTCACCTGGAGGGGCACTT
                    ************************************************************

HsCD00040270        ATGCAGGGAGGAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAA
NM_005316.3         ATGCAGGGAGGAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAA
NM_001142307.1      ATGCAGGGAGGAACACAGCAAGCCATAAACCAGATGGTGCCAAATGATATTCAATCTGAA
                    ************************************************************

HsCD00040270        TTGAAACACTTATATGTAGCTGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCT
NM_005316.3         TTGAAACACTTATATGTAGCTGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCT
NM_001142307.1      TTGAAACACTTATATGTAGCTGTTGGAGAACTTCTACGACATTTCTGGTCCTGCTTTCCT
                    ************************************************************

HsCD00040270        GTTAATACGCCATTCCTAGAAGAAAAGGTAGTGAAAATGAAAAGTAATTTGGAACGATTC
NM_005316.3         GTTAATACGCCATTCCTAGAAGAAAAGGTAGTGAAAATGAAAAGTAATTTGGAACGATTC
NM_001142307.1      GTTAATACGCCATTCCTAGAAGAAAAGGTAGTGAAAATGAAAAGTAATTTGGAACGATTC
                    ************************************************************

HsCD00040270        CAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGAGACAGTATTTAAGCACAAAT
NM_005316.3         CAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGAGACAGTATTTAAGCACAAAT
NM_001142307.1      CAAGTTACGAAGCTCTGTCCATTCCAAGAAAAGATTCGGAGACAGTATTTAAGCACAAAT
                    ************************************************************

HsCD00040270        TTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCACACATGGCAG
NM_005316.3         TTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCACACATGGCAG
NM_001142307.1      TTGGTAAGTCACATAGAAGAGATGCTCCAGACAGCCTACAACAAGCTCCACACATGGCAG
                    ************************************************************

HsCD00040270        TCACGGCGTCTGATGAAGAAAACGTTG
NM_005316.3         TCACGGCGTCTGATGAAGAAAACGTGA
NM_001142307.1      TCACGGCGTCTGATGAAGAAAACGTGA
                    ************************* .

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : DNA Repair
Reactome : Disease
Reactome : Dual incision reaction in GG-NER
Reactome : Dual incision reaction in TC-NER
Reactome : Epigenetic regulation of gene expression
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of incision complex in GG-NER
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Formation of transcription-coupled NER (TC-NER) repair complex
Reactome : Gene Expression
Reactome : Global Genomic NER (GG-NER)
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : Late Phase of HIV Life Cycle
Reactome : Negative epigenetic regulation of rRNA expression
Reactome : NoRC negatively regulates rRNA expression
Reactome : Nucleotide Excision Repair
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase I Chain Elongation
Reactome : RNA Polymerase I Promoter Clearance
Reactome : RNA Polymerase I Promoter Escape
Reactome : RNA Polymerase I Transcription
Reactome : RNA Polymerase I Transcription Initiation
Reactome : RNA Polymerase I Transcription Termination
Reactome : RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Transcription-coupled NER (TC-NER)
Reactome : mRNA Capping
WikiPathway : Eukaryotic Transcription Initiation

Clone

Cloning Information : 176643
HIP Master Clone ID : 151788
Original Clone ID : FLH176643.01L

Organism


TAX_ID : 9606
Species Specific ID: 2965

Genome

Chromosome : 11
Map Location : 11p15.1-p14
Ensembl : ENSG00000110768

Reagents

Labome : GTF2H1-antibody