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CYP4F11 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP4F11
Gene Name:  cytochrome P450, family 4, subfamily F, polypeptide 11
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177218.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040336

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1575nts         Open reading frame : 1 to 1575
Reference Sequence Alignment

ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
TTATGCCACCCTGACATCATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCCACT
CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
GACCCCTTCCGTTTCAACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
GTCCTGGCGCTCACGCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
GCGAACTCACAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1611
Start on reference sequence 37
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
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  • Protein
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  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP4F11
Symbol Nomenclature : CYP4F11
SYNONYM : CYPIVF11
Designation : cytochrome P450 4F11|cytochrome P450, subfamily IVF, polypeptide 11
Full Nomenclature : cytochrome P450, family 4, subfamily F, polypeptide 11
GENEID : 57834
HGNC : 13265
MIM : 611517
Vega : OTTHUMG00000182294
Target GenBank: BC016853

 

Reference Sequence Alignment

  

HsCD00040336        ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
NM_001128932.1      ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
NM_021187.3         ATGCCGCAGCTGAGCCTGTCCTGGCTGGGCCTCGGGCCCGTGGCAGCATCCCCGTGGCTG
                    ************************************************************

HsCD00040336        CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
NM_001128932.1      CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
NM_021187.3         CTTCTGCTGCTGGTTGGGGGCTCCTGGCTCCTGGCCCGCGTCCTGGCCTGGACCTACACC
                    ************************************************************

HsCD00040336        TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
NM_001128932.1      TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
NM_021187.3         TTCTATGACAACTGCCGCCGCCTCCAGTGTTTTCCTCAACCCCCGAAACAGAACTGGTTT
                    ************************************************************

HsCD00040336        TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
NM_001128932.1      TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
NM_021187.3         TGGGGACACCAGGGCCTGGTCACTCCCACGGAAGAGGGCATGAAGACATTGACCCAGCTG
                    ************************************************************

HsCD00040336        GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
NM_001128932.1      GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
NM_021187.3         GTGACCACATATCCCCAGGGCTTTAAGTTGTGGCTGGGTCCTACCTTCCCCCTCCTCATT
                    ************************************************************

HsCD00040336        TTATGCCACCCTGACATCATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
NM_001128932.1      TTATGCCACCCTGACATTATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
NM_021187.3         TTATGCCACCCTGACATTATCCGGCCTATCACCAGTGCCTCAGCTGCTGTCGCACCCAAG
                    ***************** ******************************************

HsCD00040336        GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
NM_001128932.1      GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
NM_021187.3         GATATGATTTTCTATGGCTTCCTGAAGCCCTGGCTGGGGGATGGGCTCCTGCTGAGTGGT
                    ************************************************************

HsCD00040336        GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
NM_001128932.1      GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
NM_021187.3         GGTGACAAGTGGAGCCGCCACCGTCGGATGTTGACGCCTGCCTTCCATTTCAACATCTTG
                    ************************************************************

HsCD00040336        AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
NM_001128932.1      AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
NM_021187.3         AAGCCTTATATGAAGATTTTCAACAAGAGTGTGAACATCATGCACGACAAGTGGCAGCGC
                    ************************************************************

HsCD00040336        CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
NM_001128932.1      CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
NM_021187.3         CTGGCCTCAGAGGGCAGCGCCAGACTGGACATGTTTGAACACATCAGCCTCATGACCTTG
                    ************************************************************

HsCD00040336        GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
NM_001128932.1      GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
NM_021187.3         GACAGTCTGCAGAAATGTGTCTTCAGCTTTGAAAGCAATTGTCAGGAGAAGCCCAGTGAA
                    ************************************************************

HsCD00040336        TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
NM_001128932.1      TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
NM_021187.3         TATATTGCCGCCATCTTGGAGCTCAGTGCCTTTGTAGAAAAGAGAAACCAGCAGATTCTC
                    ************************************************************

HsCD00040336        TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
NM_001128932.1      TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
NM_021187.3         TTGCACACGGACTTCCTGTATTATCTCACTCCTGATGGGCAGCGCTTCCGCAGGGCCTGC
                    ************************************************************

HsCD00040336        CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCCGCACCCTCCCCACT
NM_001128932.1      CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCTGCACCCTCCCCACT
NM_021187.3         CACCTGGTGCACGACTTCACAGATGCCGTCATCCAGGAGCGGCGCTGCACCCTCCCCACT
                    ********************************************* **************

HsCD00040336        CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
NM_001128932.1      CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
NM_021187.3         CAGGGTATTGATGATTTCCTCAAGAACAAGGCAAAGTCCAAGACTTTAGACTTCATTGAT
                    ************************************************************

HsCD00040336        GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
NM_001128932.1      GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
NM_021187.3         GTGCTTCTGCTGAGCAAGGATGAAGATGGGAAGGAATTGTCTGATGAGGACATAAGAGCA
                    ************************************************************

HsCD00040336        GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
NM_001128932.1      GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
NM_021187.3         GAAGCTGACACCTTCATGTTTGAGGGCCATGACACTACAGCCAGTGGTCTCTCCTGGGTC
                    ************************************************************

HsCD00040336        CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
NM_001128932.1      CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
NM_021187.3         CTATACCACCTTGCAAAGCACCCAGAATACCAGGAACAGTGCCGGCAAGAAGTGCAAGAG
                    ************************************************************

HsCD00040336        CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
NM_001128932.1      CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
NM_021187.3         CTTCTGAAGGACCGTGAACCTATAGAGATTGAATGGGACGACCTGGCCCAGCTGCCCTTC
                    ************************************************************

HsCD00040336        CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
NM_001128932.1      CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
NM_021187.3         CTGACCATGTGCATTAAGGAGAGCCTGCGGTTGCATCCCCCAGTCCCGGTCATCTCCCGA
                    ************************************************************

HsCD00040336        TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
NM_001128932.1      TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
NM_021187.3         TGTTGCACGCAGGACTTTGTGCTCCCAGACGGCCGCGTCATCCCCAAAGGCATTGTCTGC
                    ************************************************************

HsCD00040336        CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
NM_001128932.1      CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
NM_021187.3         CTCATCAATATTATCGGGATCCATTACAACCCAACTGTGTGGCCAGACCCTGAGGTCTAC
                    ************************************************************

HsCD00040336        GACCCCTTCCGTTTCAACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
NM_001128932.1      GACCCCTTCCGTTTCGACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
NM_021187.3         GACCCCTTCCGTTTCGACCAAGAGAACATCAAGGAGAGGTCACCTCTGGCTTTTATTCCC
                    ***************.********************************************

HsCD00040336        TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
NM_001128932.1      TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
NM_021187.3         TTCTCGGCAGGGCCCAGAAACTGCATCGGGCAGGCGTTCGCCATGGCTGAGATGAAGGTG
                    ************************************************************

HsCD00040336        GTCCTGGCGCTCACGCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
NM_001128932.1      GTCCTGGCGCTCACCCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
NM_021187.3         GTCCTGGCGCTCACCCTGCTGCACTTCCGCATCCTGCCGACCCACACTGAACCCCGCAGG
                    ************** *********************************************

HsCD00040336        AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
NM_001128932.1      AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
NM_021187.3         AAACCCGAGCTGATATTGCGCGCAGAGGGTGGACTTTGGCTGCGGGTGGAGCCCCTGGGT
                    ************************************************************

HsCD00040336        GCGAACTCACAGTTG
NM_001128932.1      GCGAACTCACAGTGA
NM_021187.3         GCGAACTCACAGTGA
                    ************* .

Pathways

Reactome : Arachidonic acid metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Eicosanoids
Reactome : Fatty acids
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of Leukotrienes (LT) and Eoxins (EX)
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

UniProt : Q9HBI6
HPRD : 07101

Clone

Cloning Information : 177218
HIP Master Clone ID : 152363
Original Clone ID : FLH177218.01L

Organism


TAX_ID : 9606
Species Specific ID: 57834

Genome

Chromosome : 19
Map Location : 19p13.1
Ensembl : ENSG00000171903

Reagents

Labome : CYP4F11-antibody