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OPN5 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN5
Gene Name:  opsin 5
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072508)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403364

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1062nts         Open reading frame : 1 to 1062
Reference Sequence Alignment

ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN5
Symbol Nomenclature : OPN5
SYNONYM : GPR136; PGR12; TMEM13
Designation : G-protein coupled receptor 136|neuropsin|opsin-5|transmembrane protein 13
Full Nomenclature : opsin 5
GENEID : 221391
GenBank Accession : HQ258199
HGNC : 19992
MIM : 609042
Target GenBank: HQ258199

 

Reference Sequence Alignment

  

HsCD00403364      ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
NM_181744.3       ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
                  ************************************************************

HsCD00403364      GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
NM_181744.3       GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
                  ************************************************************

HsCD00403364      ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
NM_181744.3       ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
                  ************************************************************

HsCD00403364      CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
NM_181744.3       CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
                  ************************************************************

HsCD00403364      GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
NM_181744.3       GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
                  ************************************************************

HsCD00403364      TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
NM_181744.3       TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
                  ************************************************************

HsCD00403364      CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
NM_181744.3       CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
                  ************************************************************

HsCD00403364      GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
NM_181744.3       GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
                  ************************************************************

HsCD00403364      TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
NM_181744.3       TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
                  ************************************************************

HsCD00403364      ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
NM_181744.3       ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
                  ************************************************************

HsCD00403364      CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
NM_181744.3       CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
                  ************************************************************

HsCD00403364      AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
NM_181744.3       AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
                  ************************************************************

HsCD00403364      CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
NM_181744.3       CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
                  ************************************************************

HsCD00403364      TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
NM_181744.3       TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
                  ************************************************************

HsCD00403364      GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
NM_181744.3       GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
                  ************************************************************

HsCD00403364      TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
NM_181744.3       TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
                  ************************************************************

HsCD00403364      TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
NM_181744.3       TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
                  ************************************************************

HsCD00403364      AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA---
NM_181744.3       AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAATAA
                  ******************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

HPRD : 12358

Clone

Cloning Information : 572592

Organism


TAX_ID : 9606
Species Specific ID: 221391

Genome

Chromosome : 6
Map Location : 6p12.3

Reagents

Labome : OPN5-antibody