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GALNACT-2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSGALNACT2
Gene Name:  chondroitin sulfate N-acetylgalactosaminyltransferase 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH177243.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040339

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1629nts         Open reading frame : 1 to 1629
Reference Sequence Alignment

ATGCCTAGAAGAGGACTGATTCTTCACACCCGGACCCACTGGTTGCTGTTGGGCCTTGCT
TTGCTCTGCAGTTTGGTATTATTTATGTACCTCCTGGAATGTGCCCCCCAGACTGATGGA
AATGCATCTCTTCCTGGTGTTGTTGGGGAAAATTATGGTAAAGAGTATTATCAAGCCCTC
CTACAGGAACAAGAAGAACATTATCAGACCAGGGCAACCAGTCTGAAACGCCAAATTGCC
CAACTAAAACAAGAATTACAAGAAATGAGTGAGAAGATGCGGTCACTGCAAGAAAGAAGG
AATGTAGGGGCTAATGGCATAGGCTATCAGAGCAACAAAGAGCAAGCACCTAGTGATCTT
TTAGAGTTTCTTCATTCCCAAATTGACAAAGCTGAAGTTAGCATAGGGGCCAAACTACCC
AGTGAGTATGGGGTCATTCCCTTTGAAAGTTTTACCTTAATGAAAGTATTTCAATTGGAA
ATGGGTCTCACTCGCCATCCTGAAGAAAAGCCAGTTAGAAAAGACAAACGAGATGAATTG
GTGGAAGTTATTGAAGCGGGCTTGGAGGTCATTAATAATCCTGATGAAGATGATGAACAA
GAAGATGAGGAGGGTCCCCTTGGAGAGAAACTGATATTTAATGAAAATGACTTCGTAGAA
GGTTATTATCGCACTGAGAGAGATAAGGGCACACAGTATGAACTCTTTTTTAAGAAAGCA
GACCTTACGGAATATAGACATGTGACCCTCTTCCGCCCTTTTGGACCTCTCATGAAAGTG
AAGAGTGAGATGATTGACATCACTAGATCAATTATTAATATCATTGTGCCACTTGCTGAA
AGAACTGAAGCATTTGTACAATTTATGCAGAACTTCAGGGATGTTTGTATTCATCAAGAC
AAGAAGATTCATCTCACAGTGGTGTATTTTGGTAAAGAAGGACTGTCTAAAGTCAAGTCT
ATCCTAGAATCTGTCACCAGTGAGTCTAATTTTCACAATTACACCTTGGTCTCATTGAAT
GAAGAATTTAATCGTGGACGAGGACTAAATGTGGGTGCCCGAGCTTGGGACAAGGGAGAG
GTCTTGATGTTTTTCTGTGATGTTGATATCTATTTCTCAGCCGAATTCCTTAACAGCTGC
CGGTTAAATGCTGAGCCAGGTAAGAAGGTGTTTTACCCTGTGGTGTTCAGTCTTTACAAT
CCTGCCATTGTTTATGCCAACCAGGAAGTGCCACCACCTGTGGAGCAGCAGCTGGTTCAC
AAAAAGGATTCTGGCTTTTGGCGAGATTTTGGCTTTGGAATGACTTGTCAGTATCGTTCA
GATTTCCTGACCATTGGTGGATTTGACATGGAAGTGAAAGGTTGGGGTGGAGAAGATGTT
CATCTTTATCGAAAATACTTACATGGTGACCTCATTGTGATTCGGACTCCGGTTCCTGGT
CTTTTCCACCTCTGGCATGAAAAGCGCTGTGCTGATGAGCTGACCCCCGAGCAGTACCGC
ATGTGCATCCAGTCTAAAGCCATGAATGAGGCCTCTCACTCCCACCTGGGAATGCTGGTC
TTCAGGGAGGAAATAGAGACGCATCTTCATAAACAGGCATACAGGACAAACAGTGAAGCT
GTTGGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1964
Start on reference sequence 336
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSGALNACT2
Symbol Nomenclature : CSGALNACT2
SYNONYM : CHGN2; GALNACT-2; GALNACT2
Designation : beta 4 GalNAcT-2|chondroitin beta1,4 N-acetylgalactosaminyltransferase 2
Full Nomenclature : chondroitin sulfate N-acetylgalactosaminyltransferase 2
GENEID : 55454
Locus Tag : PRO0082
HGNC : 24292
Vega : OTTHUMG00000018023
Target GenBank: BC030268

 

Reference Sequence Alignment

  

HsCD00040339      ATGCCTAGAAGAGGACTGATTCTTCACACCCGGACCCACTGGTTGCTGTTGGGCCTTGCT
NM_018590.4       ATGCCTAGAAGAGGACTGATTCTTCACACCCGGACCCACTGGTTGCTGTTGGGCCTTGCT
                  ************************************************************

HsCD00040339      TTGCTCTGCAGTTTGGTATTATTTATGTACCTCCTGGAATGTGCCCCCCAGACTGATGGA
NM_018590.4       TTGCTCTGCAGTTTGGTATTATTTATGTACCTCCTGGAATGTGCCCCCCAGACTGATGGA
                  ************************************************************

HsCD00040339      AATGCATCTCTTCCTGGTGTTGTTGGGGAAAATTATGGTAAAGAGTATTATCAAGCCCTC
NM_018590.4       AATGCATCTCTTCCTGGTGTTGTTGGGGAAAATTATGGTAAAGAGTATTATCAAGCCCTC
                  ************************************************************

HsCD00040339      CTACAGGAACAAGAAGAACATTATCAGACCAGGGCAACCAGTCTGAAACGCCAAATTGCC
NM_018590.4       CTACAGGAACAAGAAGAACATTATCAGACCAGGGCAACCAGTCTGAAACGCCAAATTGCC
                  ************************************************************

HsCD00040339      CAACTAAAACAAGAATTACAAGAAATGAGTGAGAAGATGCGGTCACTGCAAGAAAGAAGG
NM_018590.4       CAACTAAAACAAGAATTACAAGAAATGAGTGAGAAGATGCGGTCACTGCAAGAAAGAAGG
                  ************************************************************

HsCD00040339      AATGTAGGGGCTAATGGCATAGGCTATCAGAGCAACAAAGAGCAAGCACCTAGTGATCTT
NM_018590.4       AATGTAGGGGCTAATGGCATAGGCTATCAGAGCAACAAAGAGCAAGCACCTAGTGATCTT
                  ************************************************************

HsCD00040339      TTAGAGTTTCTTCATTCCCAAATTGACAAAGCTGAAGTTAGCATAGGGGCCAAACTACCC
NM_018590.4       TTAGAGTTTCTTCATTCCCAAATTGACAAAGCTGAAGTTAGCATAGGGGCCAAACTACCC
                  ************************************************************

HsCD00040339      AGTGAGTATGGGGTCATTCCCTTTGAAAGTTTTACCTTAATGAAAGTATTTCAATTGGAA
NM_018590.4       AGTGAGTATGGGGTCATTCCCTTTGAAAGTTTTACCTTAATGAAAGTATTTCAATTGGAA
                  ************************************************************

HsCD00040339      ATGGGTCTCACTCGCCATCCTGAAGAAAAGCCAGTTAGAAAAGACAAACGAGATGAATTG
NM_018590.4       ATGGGTCTCACTCGCCATCCTGAAGAAAAGCCAGTTAGAAAAGACAAACGAGATGAATTG
                  ************************************************************

HsCD00040339      GTGGAAGTTATTGAAGCGGGCTTGGAGGTCATTAATAATCCTGATGAAGATGATGAACAA
NM_018590.4       GTGGAAGTTATTGAAGCGGGCTTGGAGGTCATTAATAATCCTGATGAAGATGATGAACAA
                  ************************************************************

HsCD00040339      GAAGATGAGGAGGGTCCCCTTGGAGAGAAACTGATATTTAATGAAAATGACTTCGTAGAA
NM_018590.4       GAAGATGAGGAGGGTCCCCTTGGAGAGAAACTGATATTTAATGAAAATGACTTCGTAGAA
                  ************************************************************

HsCD00040339      GGTTATTATCGCACTGAGAGAGATAAGGGCACACAGTATGAACTCTTTTTTAAGAAAGCA
NM_018590.4       GGTTATTATCGCACTGAGAGAGATAAGGGCACACAGTATGAACTCTTTTTTAAGAAAGCA
                  ************************************************************

HsCD00040339      GACCTTACGGAATATAGACATGTGACCCTCTTCCGCCCTTTTGGACCTCTCATGAAAGTG
NM_018590.4       GACCTTACGGAATATAGACATGTGACCCTCTTCCGCCCTTTTGGACCTCTCATGAAAGTG
                  ************************************************************

HsCD00040339      AAGAGTGAGATGATTGACATCACTAGATCAATTATTAATATCATTGTGCCACTTGCTGAA
NM_018590.4       AAGAGTGAGATGATTGACATCACTAGATCAATTATTAATATCATTGTGCCACTTGCTGAA
                  ************************************************************

HsCD00040339      AGAACTGAAGCATTTGTACAATTTATGCAGAACTTCAGGGATGTTTGTATTCATCAAGAC
NM_018590.4       AGAACTGAAGCATTTGTACAATTTATGCAGAACTTCAGGGATGTTTGTATTCATCAAGAC
                  ************************************************************

HsCD00040339      AAGAAGATTCATCTCACAGTGGTGTATTTTGGTAAAGAAGGACTGTCTAAAGTCAAGTCT
NM_018590.4       AAGAAGATTCATCTCACAGTGGTGTATTTTGGTAAAGAAGGACTGTCTAAAGTCAAGTCT
                  ************************************************************

HsCD00040339      ATCCTAGAATCTGTCACCAGTGAGTCTAATTTTCACAATTACACCTTGGTCTCATTGAAT
NM_018590.4       ATCCTAGAATCTGTCACCAGTGAGTCTAATTTTCACAATTACACCTTGGTCTCATTGAAT
                  ************************************************************

HsCD00040339      GAAGAATTTAATCGTGGACGAGGACTAAATGTGGGTGCCCGAGCTTGGGACAAGGGAGAG
NM_018590.4       GAAGAATTTAATCGTGGACGAGGACTAAATGTGGGTGCCCGAGCTTGGGACAAGGGAGAG
                  ************************************************************

HsCD00040339      GTCTTGATGTTTTTCTGTGATGTTGATATCTATTTCTCAGCCGAATTCCTTAACAGCTGC
NM_018590.4       GTCTTGATGTTTTTCTGTGATGTTGATATCTATTTCTCAGCCGAATTCCTTAACAGCTGC
                  ************************************************************

HsCD00040339      CGGTTAAATGCTGAGCCAGGTAAGAAGGTGTTTTACCCTGTGGTGTTCAGTCTTTACAAT
NM_018590.4       CGGTTAAATGCTGAGCCAGGTAAGAAGGTGTTTTACCCTGTGGTGTTCAGTCTTTACAAT
                  ************************************************************

HsCD00040339      CCTGCCATTGTTTATGCCAACCAGGAAGTGCCACCACCTGTGGAGCAGCAGCTGGTTCAC
NM_018590.4       CCTGCCATTGTTTATGCCAACCAGGAAGTGCCACCACCTGTGGAGCAGCAGCTGGTTCAC
                  ************************************************************

HsCD00040339      AAAAAGGATTCTGGCTTTTGGCGAGATTTTGGCTTTGGAATGACTTGTCAGTATCGTTCA
NM_018590.4       AAAAAGGATTCTGGCTTTTGGCGAGATTTTGGCTTTGGAATGACTTGTCAGTATCGTTCA
                  ************************************************************

HsCD00040339      GATTTCCTGACCATTGGTGGATTTGACATGGAAGTGAAAGGTTGGGGTGGAGAAGATGTT
NM_018590.4       GATTTCCTGACCATTGGTGGATTTGACATGGAAGTGAAAGGTTGGGGTGGAGAAGATGTT
                  ************************************************************

HsCD00040339      CATCTTTATCGAAAATACTTACATGGTGACCTCATTGTGATTCGGACTCCGGTTCCTGGT
NM_018590.4       CATCTTTATCGAAAATACTTACATGGTGACCTCATTGTGATTCGGACTCCGGTTCCTGGT
                  ************************************************************

HsCD00040339      CTTTTCCACCTCTGGCATGAAAAGCGCTGTGCTGATGAGCTGACCCCCGAGCAGTACCGC
NM_018590.4       CTTTTCCACCTCTGGCATGAAAAGCGCTGTGCTGATGAGCTGACCCCCGAGCAGTACCGC
                  ************************************************************

HsCD00040339      ATGTGCATCCAGTCTAAAGCCATGAATGAGGCCTCTCACTCCCACCTGGGAATGCTGGTC
NM_018590.4       ATGTGCATCCAGTCTAAAGCCATGAATGAGGCCTCTCACTCCCACCTGGGAATGCTGGTC
                  ************************************************************

HsCD00040339      TTCAGGGAGGAAATAGAGACGCATCTTCATAAACAGGCATACAGGACAAACAGTGAAGCT
NM_018590.4       TTCAGGGAGGAAATAGAGACGCATCTTCATAAACAGGCATACAGGACAAACAGTGAAGCT
                  ************************************************************

HsCD00040339      GTTGGTTTG
NM_018590.4       GTTGGTTGA
                  ******* .

Pathways

Reactome : Chondroitin sulfate biosynthesis
Reactome : Chondroitin sulfate/dermatan sulfate metabolism
Reactome : Disease
Reactome : Glycogen storage diseases
Reactome : Glycosaminoglycan metabolism
Reactome : MPS I - Hurler syndrome
Reactome : MPS II - Hunter syndrome
Reactome : MPS IIIA - Sanfilippo syndrome A
Reactome : MPS IIIB - Sanfilippo syndrome B
Reactome : MPS IIIC - Sanfilippo syndrome C
Reactome : MPS IIID - Sanfilippo syndrome D
Reactome : MPS IV - Morquio syndrome A
Reactome : MPS IV - Morquio syndrome B
Reactome : MPS IX - Natowicz syndrome
Reactome : MPS VI - Maroteaux-Lamy syndrome
Reactome : MPS VII - Sly syndrome
Reactome : Metabolism
Reactome : Metabolism of carbohydrates
Reactome : Mucopolysaccharidoses
Reactome : Myoclonic epilepsy of Lafora

Protein

UniProt : Q8N6G5
HPRD : 11348

Clone

Cloning Information : 177243
HIP Master Clone ID : 152388
Original Clone ID : FLH177243.01L

Organism


TAX_ID : 9606
Species Specific ID: 55454

Genome

Chromosome : 10
Map Location : 10q11.21
Ensembl : ENSG00000169826

Reagents

Labome : CSGALNACT2-antibody