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CYP2U1 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP2U1
Gene Name:  cytochrome P450, family 2, subfamily U, polypeptide 1
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073170)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403467

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1632nts         Open reading frame : 1 to 1632
Reference Sequence Alignment

ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
AAGGAGAAGGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTC
TCTCATTCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATT
GAGGAGTTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCT
TTCTCCATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGC
TTTGATTACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAA
ATCTGTCTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCC
TTTGGACCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAA
ATCATCAAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATG
TACCTTCTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAG
TACTTATTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCT
TTGCTCTGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAA
GAAATTGAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATG
CCCTACACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCC
ATTCCTCATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACA
TTGATCTTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAG
GATTTCTACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTT
ATTCCTTTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTA
TTCCTAATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAG
AAGCCCCTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACT
ATTTCAAGGAGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP2U1
Symbol Nomenclature : CYP2U1
SYNONYM : P450TEC; SPG49; SPG56
Designation : cytochrome P450 2U1|spastic paraplegia 49
Full Nomenclature : cytochrome P450, family 2, subfamily U, polypeptide 1
GENEID : 113612
GenBank Accession : HQ258621
HGNC : 20582
MIM : 610670
Vega : OTTHUMG00000161084
Target GenBank: HQ258621

 

Reference Sequence Alignment

  

HsCD00403467        ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
NM_183075.2         ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
XM_005262717.1      ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
                    ************************************************************

HsCD00403467        CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
NM_183075.2         CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
XM_005262717.1      CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
                    ************************************************************

HsCD00403467        GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
NM_183075.2         GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
XM_005262717.1      GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
                    ************************************************************

HsCD00403467        CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
NM_183075.2         CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
XM_005262717.1      CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
                    ************************************************************

HsCD00403467        CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
NM_183075.2         CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
XM_005262717.1      CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
                    ************************************************************

HsCD00403467        ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
NM_183075.2         ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
XM_005262717.1      ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
                    ************************************************************

HsCD00403467        TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
NM_183075.2         TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
XM_005262717.1      TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
                    ************************************************************

HsCD00403467        GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
NM_183075.2         GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
XM_005262717.1      GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
                    ************************************************************

HsCD00403467        AAGGAGAAGG--------------------------------------------------
NM_183075.2         AAGGAGAAGG--------------------------------------------------
XM_005262717.1      AAGGAGAAGGGTGAGCGGGAGGTCGTGGGCTGTGGGTACGCGGATGCCGCGGATGAGTCT
                    **********                                                  

HsCD00403467        ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
NM_183075.2         ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
XM_005262717.1      CCAGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
                        ********************************************************

HsCD00403467        TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
NM_183075.2         TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
XM_005262717.1      TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
                    ************************************************************

HsCD00403467        TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
NM_183075.2         TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
XM_005262717.1      TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
                    ************************************************************

HsCD00403467        ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
NM_183075.2         ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
XM_005262717.1      ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
                    ************************************************************

HsCD00403467        TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
NM_183075.2         TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
XM_005262717.1      TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
                    ************************************************************

HsCD00403467        CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
NM_183075.2         CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
XM_005262717.1      CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
                    ************************************************************

HsCD00403467        CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
NM_183075.2         CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
XM_005262717.1      CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
                    ************************************************************

HsCD00403467        AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
NM_183075.2         AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
XM_005262717.1      AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
                    ************************************************************

HsCD00403467        CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
NM_183075.2         CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
XM_005262717.1      CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
                    ************************************************************

HsCD00403467        TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
NM_183075.2         TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
XM_005262717.1      TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
                    ************************************************************

HsCD00403467        TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
NM_183075.2         TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
XM_005262717.1      TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
                    ************************************************************

HsCD00403467        GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
NM_183075.2         GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
XM_005262717.1      GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
                    ************************************************************

HsCD00403467        ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
NM_183075.2         ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
XM_005262717.1      ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
                    ************************************************************

HsCD00403467        CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
NM_183075.2         CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
XM_005262717.1      CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
                    ************************************************************

HsCD00403467        TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
NM_183075.2         TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
XM_005262717.1      TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
                    ************************************************************

HsCD00403467        TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
NM_183075.2         TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
XM_005262717.1      TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
                    ************************************************************

HsCD00403467        TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
NM_183075.2         TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
XM_005262717.1      TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
                    ************************************************************

HsCD00403467        ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
NM_183075.2         ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
XM_005262717.1      ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
                    ************************************************************

HsCD00403467        CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
NM_183075.2         CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
XM_005262717.1      CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
                    ************************************************************

HsCD00403467        AGGAGA---
NM_183075.2         AGGAGATGA
XM_005262717.1      AGGAGATGA
                    ******   

Pathways

Reactome : Arachidonic acid metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 13109

Clone

Cloning Information : 572723

Organism


TAX_ID : 9606
Species Specific ID: 113612

Genome

Chromosome : 4
Map Location : 4q25
Ensembl : ENSG00000155016

Reagents

Labome : CYP2U1-antibody