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FNDC7 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  FNDC7
Gene Name:  fibronectin type III domain containing 7
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072859)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00403779

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1500nts         Open reading frame : 1 to 1500
Reference Sequence Alignment

ATGGCTTTGAGCGACTCTTCAGAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATC
TCTTCCCTCCAGTGTGGAACTGAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGA
TCTAGCAAATCATCTTCAGCAATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTG
ACGATCCAAGAAGATCCCCCTGGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGT
GACTACTATGTGGTCTTTGTGAAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCT
CTCACTCAGTGCAACTTCTTATCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTC
TATAACAAGGCAGGGCAAAGTCCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGT
TGTCCTAGTGACATTAACCCCGTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCT
CCTGTCCGTGGTGCCGAACTGTATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAG
TGCAATGACACTACTCCTGCGTGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAAC
ATCACAGTGTATTCATTCAATGAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTC
ATAACCACAGCTCCTTGCAGTCCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATG
ATTAATGTGCACTGGCGATCCACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGG
GAGAAAGGACTGTATCAGTGCAGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCC
TGTGGCTCAGTGTTCTCTGTCACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCC
AGCTACAGTGTGCCCCTGGAAACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAA
ATCACCCAGTCAGTAATCAACGTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTT
GCAGTTCTGGAGTCACACACTGGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTC
CTAGGGTGCATCACATGTGGCATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACC
GGGTTGACTGCAGATTGCTCCTACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGG
GTGAAATTATATAGGCTGGGCCCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGC
TCTGCCAATTACAGCACTGACCTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGT
GCTGGCCTCAGTTTCTGTGATGTCACTGAGATACCCTGTGGGGATGTATACACTGTGATG
GTCTCACCAGTTGCTAAAACAGGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCA
GTAACTTGCTCTGGAAGTACACTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FNDC7
Symbol Nomenclature : FNDC7
SYNONYM : RP11-293A10.2
Designation : fibronectin type III domain-containing protein 7
Full Nomenclature : fibronectin type III domain containing 7
GENEID : 163479
GenBank Accession : HQ258430
HGNC : 26668
Vega : OTTHUMG00000011120
Target GenBank: HQ258430

 

Reference Sequence Alignment

  

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      ATGGCTGGTGGACGAGAGACATGTTTGCCTTTGATTGGATTCATTCTTATCTGTCTTAAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      ATGGTTGCTTCAGCAAAATCAGCTCCTGAAATACCCACTATTGATCAGGCATATTCAAAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      CTCAGCAACAGTATCACTGTAGAATGGGCTACAGTGCCGGGTGCCACCAGTTACCTCCTC
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      ACGGCTGAAGACGGGGACACAGTCATTGAAACCACGGTGGCCAATTCCCCAGGCACTGTG
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      ACGGGACTAAAGGCTGCAACCTGGTATGAAATCACCATCAGATCCATCAGCGCTGCTGGG
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      AGAAGCCAGGCGTCACCTCCAAAGCAGGCAAAGACAGTGTTGGCTGCACCAATTCTAGAA
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      GTAAGCTCTCCAAGTTCAGACTCCATTCTTGTGCAGTGGGAAGCTGTATATATGGCAATT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      GCATTCTCCGTGTCCATTATGCGAGCCAATGGCTTGGGGAGTATATGGAAAGAGAATACT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      ACAAACACCTCCTTGACATTCACCAGTTTAGAAGCCGGAACTCTCTACACCATAAAGGCC
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      TATGCATGGAATGCCAACAGAATCCCTGGGGATGACTCCACCTGCAATCAGAGAACAAGT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ------------------------------------------------------------
NM_001144937.1      CCTCGGGCCCCTGCCAACATTCAAGTCTCTTTCGATAGTGGAGCTCTGAAGGCATCTTTT
XM_005270550.1      ------------------------------------------------------------
                                                                                

HsCD00403779        ---------------------------------------ATGGCTTTGAGCGACTCTTCA
NM_001144937.1      TCCTGGGCACGGGCAGAAGGAGCTTTCAATTATACTGTGATGGCTTTGAGCGACTCTTCA
XM_005270550.1      ---------------------------------------ATGGCTTTGAGCGACTCTTCA
                                                           *********************

HsCD00403779        GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
NM_001144937.1      GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
XM_005270550.1      GAGCTGACCTGCAGTACAACTTTCAGTTCCTGCACCATCTCTTCCCTCCAGTGTGGAACT
                    ************************************************************

HsCD00403779        GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
NM_001144937.1      GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
XM_005270550.1      GAATACTTGATTTCAGTTTTAGCAAGTAATGATGCTGGATCTAGCAAATCATCTTCAGCA
                    ************************************************************

HsCD00403779        ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
NM_001144937.1      ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
XM_005270550.1      ATGACCCTGAAAACTGTTGCTTGTGCACCCGGAAGAGTGACGATCCAAGAAGATCCCCCT
                    ************************************************************

HsCD00403779        GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
NM_001144937.1      GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
XM_005270550.1      GGCCACCTGTCTGTGGCTTGGTCCAGTGTAGATCTGGGTGACTACTATGTGGTCTTTGTG
                    ************************************************************

HsCD00403779        AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
NM_001144937.1      AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
XM_005270550.1      AAGAGTGATGATGGCTTGGAAGTACACTGCAACACATCTCTCACTCAGTGCAACTTCTTA
                    ************************************************************

HsCD00403779        TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
NM_001144937.1      TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
XM_005270550.1      TCTGAGTGTGGCTTCACTTATTTTATTAGTGTTTTTGTCTATAACAAGGCAGGGCAAAGT
                    ************************************************************

HsCD00403779        CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
NM_001144937.1      CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
XM_005270550.1      CCTTTGGGTGACATATTCAATTATACCACAGCTCCCTGTTGTCCTAGTGACATTAACCCC
                    ************************************************************

HsCD00403779        GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
NM_001144937.1      GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
XM_005270550.1      GTGTTGGTGTCCAGTGACAGAGTTGAGATTGTCTGGTCTCCTGTCCGTGGTGCCGAACTG
                    ************************************************************

HsCD00403779        TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
NM_001144937.1      TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
XM_005270550.1      TATGAAACCAAGGCTGTAGATGGGTACAACATGGTTGAGTGCAATGACACTACTCCTGCG
                    ************************************************************

HsCD00403779        TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
NM_001144937.1      TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
XM_005270550.1      TGCACCCTTTCGGCTCTAGAGTGTGACACCAAGTACAACATCACAGTGTATTCATTCAAT
                    ************************************************************

HsCD00403779        GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
NM_001144937.1      GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
XM_005270550.1      GAAGTCCGAGGCAGCAATATGTCATGTACTCCCCAGTTCATAACCACAGCTCCTTGCAGT
                    ************************************************************

HsCD00403779        CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
NM_001144937.1      CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
XM_005270550.1      CCTGAAATAAAAAATGTTTCAAGGGATGCATTCTCCATGATTAATGTGCACTGGCGATCC
                    ************************************************************

HsCD00403779        ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
NM_001144937.1      ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
XM_005270550.1      ACTAATGATGATGCTACTTACACGGTGACTGCCCAAGGGGAGAAAGGACTGTATCAGTGC
                    ************************************************************

HsCD00403779        AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
NM_001144937.1      AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
XM_005270550.1      AGCAGCACAGGAGAGTCCTGCACCATGCGGGGCTTGCCCTGTGGCTCAGTGTTCTCTGTC
                    ************************************************************

HsCD00403779        ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
NM_001144937.1      ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
XM_005270550.1      ACTGCTGTGGCCGAAACACAGGCAGGACGGAGCCTGCCCAGCTACAGTGTGCCCCTGGAA
                    ************************************************************

HsCD00403779        ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
NM_001144937.1      ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
XM_005270550.1      ACAGTGCCATGCTGTCCAACCGGTCTGACAGTAACTCAAATCACCCAGTCAGTAATCAAC
                    ************************************************************

HsCD00403779        GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
NM_001144937.1      GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
XM_005270550.1      GTGAGCTGGACTATTGGGAGAGTGGCTCAAACCCATGTTGCAGTTCTGGAGTCACACACT
                    ************************************************************

HsCD00403779        GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGGTGCATCACATGTGGC
NM_001144937.1      GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGATGCATCACATGTGGC
XM_005270550.1      GGACAGTCTAAGTGTCACACTCATCAAAACCACTGCCTCCTAGGATGCATCACATGTGGC
                    ********************************************.***************

HsCD00403779        ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
NM_001144937.1      ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
XM_005270550.1      ATCAATTACACAGTGACATTAAAAGCAATTAGTGCCACCGGGTTGACTGCAGATTGCTCC
                    ************************************************************

HsCD00403779        TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
NM_001144937.1      TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
XM_005270550.1      TACCAAAGTTATTTCTCTGGTGCCTGCTGCCCTTTGGGGGTGAAATTATATAGGCTGGGC
                    ************************************************************

HsCD00403779        CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
NM_001144937.1      CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
XM_005270550.1      CCTAATGGCATCCGGATCTACTGGCAAGCCTCCAGGGGCTCTGCCAATTACAGCACTGAC
                    ************************************************************

HsCD00403779        CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
NM_001144937.1      CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
XM_005270550.1      CTCTATGGCTCCAAAGGCATTTTCACGTGCACCCCGAGTGCTGGCCTCAGTTTCTGTGAT
                    ************************************************************

HsCD00403779        GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
NM_001144937.1      GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
XM_005270550.1      GTCACTGAGATACCCTGTGGGGATGTATACACTGTGATGGTCTCACCAGTTGCTAAAACA
                    ************************************************************

HsCD00403779        GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTAACTTGCTCTGGAAGTACA
NM_001144937.1      GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTAACTTGCTCTGGAAGTACA
XM_005270550.1      GGATTGAAGCTTACTTTCTGTCCAAAAAAAATATATTCAGTGATTTATAGAGGGAAGAGA
                    *****************************************.* **. : :**.*. * *

HsCD00403779        CTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAA---
NM_001144937.1      CTTGGAATGGTGATTTATAGAGGGAAGAGAAATGAAGAATGA
XM_005270550.1      AATGAAGAATGA------------------------------
                    .:**.*.:.  .                              

Protein

SMART domain : FN3 : Fibronectin type 3 domain
HPRD : 08195

Clone

Cloning Information : 574617

Organism


TAX_ID : 9606
Species Specific ID: 163479

Genome

Chromosome : 1
Map Location : 1p13.3
Ensembl : ENSG00000143107

Reagents

Labome : FNDC7-antibody