DNASU Plasmid Repository • 480.965.5697 | Email

KBTBD3 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  KBTBD3
Gene Name:  kelch repeat and BTB (POZ) domain containing 3
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100072631)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404044

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1836nts         Open reading frame : 1 to 1836
Reference Sequence Alignment

ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
AAATACAGAGACCCATGGTTTTCTAATCTATGTGCT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KBTBD3
Symbol Nomenclature : KBTBD3
SYNONYM : BKLHD3
Designation : BTB and kelch domain containing 3|BTB and kelch domain-containing protein 3|kelch repeat and BTB domain-containing protein 3
Full Nomenclature : kelch repeat and BTB (POZ) domain containing 3
GENEID : 143879
GenBank Accession : HQ258322
HGNC : 22934
Vega : OTTHUMG00000166251
Target GenBank: HQ258322

 

Reference Sequence Alignment

  

HsCD00404044      ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
NM_198439.2       ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
NM_152433.3       ATGGAATTGGCTATGGATAATTCATATGCTTTCAATCAACGAAGCACATGTAATGGAATT
                  ************************************************************

HsCD00404044      CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
NM_198439.2       CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
NM_152433.3       CCATCTGAGAAGAAAAACAACTTCCTTGTATCAGAAGATCATGGACAAAAAATCTTAAGT
                  ************************************************************

HsCD00404044      GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
NM_198439.2       GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
NM_152433.3       GTACTACAGAATTTTAGAGAACAAAATGTCTTTTATGATTTCAAAATAATTATGAAAGAT
                  ************************************************************

HsCD00404044      GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
NM_198439.2       GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
NM_152433.3       GAAATAATCCCGTGTCATCGTTGTGTGTTAGCAGCATGCAGTGACTTTTTCAGGGCTATG
                  ************************************************************

HsCD00404044      TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
NM_198439.2       TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
NM_152433.3       TTTGAAGTAAACATGAAAGAAAGAGATGATGGAAGTGTTACCATTACTAATTTGTCCTCC
                  ************************************************************

HsCD00404044      AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
NM_198439.2       AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
NM_152433.3       AAGGCAGTAAAAGCATTTCTCGATTATGCCTATACTGGAAAAACAAAAATAACAGATGAT
                  ************************************************************

HsCD00404044      AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
NM_198439.2       AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
NM_152433.3       AATGTGGAAATGTTCTTCCAGTTGTCATCATTTCTTCAAGTTTCCTTCCTATCCAAAGCT
                  ************************************************************

HsCD00404044      TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
NM_198439.2       TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
NM_152433.3       TGCAGTGACTTTTTAATAAAAAGTATTAATCTTGTCAATTGTTTACAGTTATTATCTATA
                  ************************************************************

HsCD00404044      TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
NM_198439.2       TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
NM_152433.3       TCAGATAGCTATGGCTCCACCAGTTTGTTTGATCATGCATTACACTTTGTACAACATCAC
                  ************************************************************

HsCD00404044      TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
NM_198439.2       TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
NM_152433.3       TTTTCTTTATTATTTAAATCCAGTGATTTCTTAGAGATGAATTTTGGAGTACTACAGAAA
                  ************************************************************

HsCD00404044      TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
NM_198439.2       TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
NM_152433.3       TGTCTGGAATCAGATGAATTAAATGTTCCTGAAGAAGAAATGGTACTGAAAGTTGTCCTT
                  ************************************************************

HsCD00404044      AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
NM_198439.2       AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
NM_152433.3       AGTTGGACTAAACATAACTTAGAATCAAGGCAAAAGTATCTGCCTCATTTGATTGAAAAA
                  ************************************************************

HsCD00404044      GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
NM_198439.2       GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
NM_152433.3       GTGAGATTACATCAGTTATCTGAGGAGACACTTCAGGACTGTCTGTTCAATGAAGAGAGT
                  ************************************************************

HsCD00404044      TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
NM_198439.2       TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
NM_152433.3       TTACTCAAAAGCACAAACTGTTTTGACATAATCATGGATGCAATTAAGTGTGTGCAAGGT
                  ************************************************************

HsCD00404044      TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
NM_198439.2       TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
NM_152433.3       TCTGGTGGACTCTTCCCTGATGCTCGACCATCCACAACTGAGAAATACATATTCATTCAC
                  ************************************************************

HsCD00404044      AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
NM_198439.2       AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
NM_152433.3       AAAACTGAGGAAAATGGAGAAAATCAATATACATTTTGCTATAACATTAAATCTGATTCA
                  ************************************************************

HsCD00404044      TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
NM_198439.2       TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
NM_152433.3       TGGAAAATACTGCCGCAATCACACCTGATTGATTTGCCAGGATCTAGTCTTTCGAGTTAC
                  ************************************************************

HsCD00404044      GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
NM_198439.2       GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
NM_152433.3       GGAGAGAAAATATTCTTGACAGGTGGTTGCAAAGGGAAATGTTGTCGAACGGTTCGACTG
                  ************************************************************

HsCD00404044      CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
NM_198439.2       CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
NM_152433.3       CATATTGCCGAGTCATATCATGATGCCACTGATCAAACCTGGTGCTACTGTCCAGTGAAA
                  ************************************************************

HsCD00404044      AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
NM_198439.2       AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
NM_152433.3       AATGATTTCTTCTTGGTATCAACTATGAAAACACCAAGAACCATGCATACATCAGTTATG
                  ************************************************************

HsCD00404044      GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
NM_198439.2       GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
NM_152433.3       GCTCTCGATAGATTATTTGTCATAGGTGGAAAAACTAGAGGATCCCGGGACATTAAAAGT
                  ************************************************************

HsCD00404044      CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
NM_198439.2       CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
NM_152433.3       CTCTTAGATGTTGAATCTTACAATCCTCTTTCCAAAGAATGGATATCTGTTAGCCCATTA
                  ************************************************************

HsCD00404044      CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
NM_198439.2       CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
NM_152433.3       CCCAGAGGCATATACTATCCAGAAGCAAGCACATGCCAAAATGTAATTTATGTTCTTGGA
                  ************************************************************

HsCD00404044      TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
NM_198439.2       TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
NM_152433.3       TCAGAGGTAGAGATTACAGATGCTTTTAACCCATCACTTGATTGCTTTTTTAAATACAAT
                  ************************************************************

HsCD00404044      GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
NM_198439.2       GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
NM_152433.3       GCTACAACTGATCAGTGGTCTGAACTAGTAGCAGAGTTTGGGCAATTTTTTCATGCAACA
                  ************************************************************

HsCD00404044      TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
NM_198439.2       TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
NM_152433.3       TTAATTAAAGCTGTACCAGTAAACTGTACACTGTATATATGTGACCTTTCCACCTATAAG
                  ************************************************************

HsCD00404044      GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
NM_198439.2       GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
NM_152433.3       GTTTATAGTTTTTGTCCAGACACTTGTGTTTGGAAAGGCGAAGGATCTTTTGAGTGTGCA
                  ************************************************************

HsCD00404044      GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
NM_198439.2       GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
NM_152433.3       GGCTTTAATGCAGGTGCAATTGGAATTGAAGATAAAATTTATATATTAGGTGGTGATTAT
                  ************************************************************

HsCD00404044      GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
NM_198439.2       GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
NM_152433.3       GCACCAGATGAAATCACAGATGAAGTGCAGGTCTACCACAGCAACAGGTCTGAATGGGAA
                  ************************************************************

HsCD00404044      GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
NM_198439.2       GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
NM_152433.3       GAAGTTTCACCAATGCCTAGAGCCTTAACAGAATTTTACTGCCAGGTGATTCAGTTTAAT
                  ************************************************************

HsCD00404044      AAATACAGAGACCCATGGTTTTCTAATCTATGTGCT---
NM_198439.2       AAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTGA
NM_152433.3       AAATACAGAGACCCATGGTTTTCTAATCTATGTGCTTGA
                  ************************************   

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
HPRD : 10005

Clone

Cloning Information : 574895

Organism


TAX_ID : 9606
Species Specific ID: 143879

Genome

Chromosome : 11
Map Location : 11q22.3
Ensembl : ENSG00000182359

Reagents

Labome : KBTBD3-antibody