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CAPN11 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN11
Gene Name:  calpain 11
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073459)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404352

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2106nts         Open reading frame : 51 to 2156
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTGGCTC
ACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAACT
TTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTCG
AGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAACT
CCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTAT
TCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGGC
TGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCCA
GAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTTG
GACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTGT
TTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCCA
AGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGACT
TCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGGC
TCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGTG
ATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTGA
CTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAATC
CCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAGG
TGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATGT
CCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGATA
CACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGCA
GAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAGT
TTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTGG
CCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGAG
CCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATTC
AGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAGA
TCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATCA
TTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACCG
AGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAGG
AAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCAG
GAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATCA
AATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAACC
TCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAAA
AACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTGA
ACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTAA
TGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTCA
TCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAGA
ATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATACC
CAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN11
Symbol Nomenclature : CAPN11
SYNONYM : calpain11
Designation : CANP 11|calcium-activated neutral proteinase 11|calpain-11
Full Nomenclature : calpain 11
GENEID : 11131
GenBank Accession : JF432283
HGNC : 1478
MIM : 604822
Vega : OTTHUMG00000014758
Target GenBank: JF432283

 

Reference Sequence Alignment

  

HsCD00404352      ------------------------------------------------------------
NM_007058.3       ATGCTGTACTCCCCAGGGCCGAGTCTTCCGGAGTCAGCAGAGAGCCTGGATGGATCACAG
                                                                              

HsCD00404352      -TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTGGCT
NM_007058.3       GAGGATAAGCCTCGGGGCTCATGTGCGGAGCCCACTTTTACTGATACGGGAATGGTGGCT
                   :  **:**  : *    * :: :. * ...* :  *: *.:.*:.  **.*********

HsCD00404352      CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
NM_007058.3       CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
                  ************************************************************

HsCD00404352      TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
NM_007058.3       TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
                  ************************************************************

HsCD00404352      GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
NM_007058.3       GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
                  ************************************************************

HsCD00404352      TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
NM_007058.3       TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
                  ************************************************************

HsCD00404352      TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
NM_007058.3       TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
                  ************************************************************

HsCD00404352      CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
NM_007058.3       CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
                  ************************************************************

HsCD00404352      AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
NM_007058.3       AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
                  ************************************************************

HsCD00404352      GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
NM_007058.3       GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
                  ************************************************************

HsCD00404352      TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
NM_007058.3       TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
                  ************************************************************

HsCD00404352      AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
NM_007058.3       AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
                  ************************************************************

HsCD00404352      TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
NM_007058.3       TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
                  ************************************************************

HsCD00404352      CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
NM_007058.3       CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
                  ************************************************************

HsCD00404352      GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
NM_007058.3       GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
                  ************************************************************

HsCD00404352      ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
NM_007058.3       ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
                  ************************************************************

HsCD00404352      CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
NM_007058.3       CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
                  ************************************************************

HsCD00404352      GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
NM_007058.3       GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
                  ************************************************************

HsCD00404352      TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
NM_007058.3       TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
                  ************************************************************

HsCD00404352      ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
NM_007058.3       ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
                  ************************************************************

HsCD00404352      AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
NM_007058.3       AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
                  ************************************************************

HsCD00404352      TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
NM_007058.3       TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
                  ************************************************************

HsCD00404352      GCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
NM_007058.3       GTCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
                  * **********************************************************

HsCD00404352      GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
NM_007058.3       GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
                  ************************************************************

HsCD00404352      CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
NM_007058.3       CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
                  ************************************************************

HsCD00404352      ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
NM_007058.3       ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
                  ************************************************************

HsCD00404352      ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
NM_007058.3       ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
                  ************************************************************

HsCD00404352      GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
NM_007058.3       GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
                  ************************************************************

HsCD00404352      GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
NM_007058.3       GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
                  ************************************************************

HsCD00404352      GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
NM_007058.3       GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
                  ************************************************************

HsCD00404352      AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
NM_007058.3       AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
                  ************************************************************

HsCD00404352      CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
NM_007058.3       CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
                  ************************************************************

HsCD00404352      AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
NM_007058.3       AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
                  ************************************************************

HsCD00404352      AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
NM_007058.3       AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
                  ************************************************************

HsCD00404352      ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
NM_007058.3       ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
                  ************************************************************

HsCD00404352      ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
NM_007058.3       ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
                  ************************************************************

HsCD00404352      AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAC
NM_007058.3       AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
                  *********************************************************** 

HsCD00404352      CCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_007058.3       -------------------------------------------------
                                                                   

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : calpain_III :
SMART domain : CysPc : Calpain-like thiol protease family.
HPRD : 05315

Clone

Cloning Information : 579285

Organism


TAX_ID : 9606
Species Specific ID: 11131

Genome

Chromosome : 6
Map Location : 6p12
Ensembl : ENSG00000137225

Reagents

Labome : CAPN11-antibody