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SRBD1 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SRBD1
Gene Name:  S1 RNA binding domain 1
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073423)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404581

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1860nts         Open reading frame : 51 to 1910
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGATTGCTA
AAGACAAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCC
AGTCATCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTC
TGCTCTACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAA
TTAACCGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGA
AGGATGAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGC
CAGAGTTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATC
CTCTTCTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAA
TGATGTTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCT
TAATGGGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTA
GTCAGATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGG
AGAAAATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAA
CTGCCTGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCAC
CACTGGATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCC
CTGAAGCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAG
CAAGGCGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAG
TTGGAATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTG
TAGAAGAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAA
GGCATATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAA
ATGGACCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCT
TCCAACAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTC
AGCAAACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTG
AGGTCACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGA
AGCCAAATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGT
TTTTGTCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAA
TAAATTCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAG
TACACACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAA
CAGATTTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTG
GGACAGTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAG
GAGTGGGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAA
CAAAGAAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCA
ACATTGACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTATGCCCAACTT
TCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRBD1
Symbol Nomenclature : SRBD1
Designation : H_NH0576F01.1|S1 RNA-binding domain-containing protein 1|WUGSC:H_NH0576F01.1
Full Nomenclature : S1 RNA binding domain 1
GENEID : 55133
GenBank Accession : JF432253
HGNC : 25521
Vega : OTTHUMG00000128814
Target GenBank: JF432253

 

Reference Sequence Alignment

  

HsCD00404581      ------------------------------------------------------------
NM_018079.4       ATGTCATCATTGCCAAGAAGAGCGAAAGTACAGGTCCAGGATGTGGTACTGAAAGATGAA
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       TTTTCTTCATTCTCTGAGTTATCATCTGCCTCTGAAGAAGATGACAAGGAAGATAGTGCC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       TGGGAGCCCCAAAAGAAAGTTCCCAGAAGCCGTAAACAGCCCCCTCCCAAGGAATCCAAA
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       CCAAAGAGGATGCCTCGGGTGAAGAAGAATGCCCCACAGATCAGTGATGGCTCAGAAGTC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       GTTGTTGTTAAGGAGGAGCTGAATAGCTCTGTGGCTATTGCTGATACTGCTTTAGAAGAC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       AGAAAAAATAAATTGGATACTGTACAGACTCTGAAAACAGCCAAGACAAAACAGAAATGT
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       GCAGCGCAGCCACATACAGTTCGAAGGACTAAAAAGCTGAAAGTTGAAGAAGAAACCAGC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       AAAGCCAGCAACTTAGAGGGTGAGAGTAATAGTTCAGAGACACCATCCACAAGCACTGTG
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       TGGGGAGGTACATGCAAGAAGGAAGAGAATGACGATGACTTTACATTTGGTCAGTCCGCT
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       TTAAAGAAAATCAAGACTGAGACATATCCTCAGGGGCAGCCTGTCAAGTTTCCAGCAAAT
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       GCCAATAGTACTAAAGAGGAGGTGGAAATGAATTGGGACATGGTACAGGTTTTATCTGAG
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       AGAACTAATATTGAACCTTGGGTTTGTGCCAACATCATTCGTCTCTTTAATGATGATAAC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       ACAATTCCCTTCATTATACGTTATAGAAAAGAGCTCATTAATAACCTTGATGCTGATTCC
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       TTGAGAGAAGTTCAGCAAACCCTAGAAGAGCTACGGGCTGTTGCAAAGAAAGTTCATAGT
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       ACAATCCAGAAAATTAAGAAGGAAGGGAAGATGTCTGAGTGCTTGTTAAAAGCCATGCTG
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       AATTGTAAAACTTTTGAAGAACTAGAACACGTGTCTGCTCCATATAAAACTGGAAGCAAA
                                                                              

HsCD00404581      ------------------------------------------------------------
NM_018079.4       GGGACTAAAGCCCAGAGAGCAAGACAGTTGGGCTTAGAAGGAGCAGCCAGGGCACTGCTT
                                                                              

HsCD00404581      -------------------------------------------------------TCCAT
NM_018079.4       GAGAAACCAGGGGAGCTCAGTCTGCTATCGTACATTAGGCCTGACGTTAAAGGGCTTTCA
                                                                         *  .:

HsCD00404581      TAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGATTGCTAAAGAC
NM_018079.4       ACGCTTCAGGATATTGAAATAGGAGTGCAGCATATTTTAGCAGATATGATTGCTAAAGAC
                  :.*  : .  :*:**.:***.  *.   :* * *::::** :*. ***************

HsCD00404581      AAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCCAGTCA
NM_018079.4       AAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCCAGTCA
                  ************************************************************

HsCD00404581      TCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTCTGCTC
NM_018079.4       TCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTCTGCTC
                  ************************************************************

HsCD00404581      TACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAATTAAC
NM_018079.4       TACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAATTAAC
                  ************************************************************

HsCD00404581      CGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGAAGGAT
NM_018079.4       CGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGAAGGAT
                  ************************************************************

HsCD00404581      GAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGCCAGAG
NM_018079.4       GAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGCCAGAG
                  ************************************************************

HsCD00404581      TTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATCCTCTT
NM_018079.4       TTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATCCTCTT
                  ************************************************************

HsCD00404581      CTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAATGATG
NM_018079.4       CTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAATGATG
                  ************************************************************

HsCD00404581      TTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCTTAATG
NM_018079.4       TTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCTTAATG
                  ************************************************************

HsCD00404581      GGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTAGTCAG
NM_018079.4       GGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTAGTCAG
                  ************************************************************

HsCD00404581      ATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGGAGAAA
NM_018079.4       ATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGGAGAAA
                  ************************************************************

HsCD00404581      ATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAACTGCC
NM_018079.4       ATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAACTGCC
                  ************************************************************

HsCD00404581      TGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCACCACTG
NM_018079.4       TGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCACCACTG
                  ************************************************************

HsCD00404581      GATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCCCTGAA
NM_018079.4       GATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCCCTGAA
                  ************************************************************

HsCD00404581      GCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAGCAAGG
NM_018079.4       GCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAGCAAGG
                  ************************************************************

HsCD00404581      CGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAGTTGGA
NM_018079.4       CGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAGTTGGA
                  ************************************************************

HsCD00404581      ATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTGTAGAA
NM_018079.4       ATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTGTAGAA
                  ************************************************************

HsCD00404581      GAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAAGGCAT
NM_018079.4       GAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAAGGCAT
                  ************************************************************

HsCD00404581      ATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAAATGGA
NM_018079.4       ATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAAATGGA
                  ************************************************************

HsCD00404581      CCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCTTCCAA
NM_018079.4       CCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCTTCCAA
                  ************************************************************

HsCD00404581      CAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTCAGCAA
NM_018079.4       CAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTCAGCAA
                  ************************************************************

HsCD00404581      ACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTGAGGTC
NM_018079.4       ACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTGAGGTC
                  ************************************************************

HsCD00404581      ACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGAAGCCA
NM_018079.4       ACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGAAGCCA
                  ************************************************************

HsCD00404581      AATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGTTTTTG
NM_018079.4       AATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGTTTTTG
                  ************************************************************

HsCD00404581      TCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAATAAAT
NM_018079.4       TCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAATAAAT
                  ************************************************************

HsCD00404581      TCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAGTACAC
NM_018079.4       TCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAGTACAC
                  ************************************************************

HsCD00404581      ACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAACAGAT
NM_018079.4       ACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAACAGAT
                  ************************************************************

HsCD00404581      TTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTGGGACA
NM_018079.4       TTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTGGGACA
                  ************************************************************

HsCD00404581      GTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAGGAGTG
NM_018079.4       GTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAGGAGTG
                  ************************************************************

HsCD00404581      GGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAACAAAG
NM_018079.4       GGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAACAAAG
                  ************************************************************

HsCD00404581      AAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCAACATT
NM_018079.4       AAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCAACATT
                  ************************************************************

HsCD00404581      GACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTATGCCCAACTTTCTTG
NM_018079.4       GACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTATGA------------
                  ***********************************************.            

HsCD00404581      TACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_018079.4       -------------------------------------
                                                       

Clone

Cloning Information : 579578

Organism


TAX_ID : 9606
Species Specific ID: 55133

Genome

Chromosome : 2
Map Location : 2p21
Ensembl : ENSG00000068784

Reagents

Labome : SRBD1-antibody