DNASU Plasmid Repository • 480.965.5697 | Email

ZNF350 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ZNF350
Gene Name:  zinc finger protein 350
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH193338.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040462

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1599nts         Open reading frame : 1 to 1599
Reference Sequence Alignment

ATGATCCAGGCCCAGGAATCCATAACACTGGAGGATGTGGCTGTGGACTTCACTTGGGAG
GAGTGGCAACTCCTGGGCGCTGCTCAGAAGGACCTGTACCGGGACGTGATGTTGGAGAAC
TACAGCAACCTGGTGGCAGTGGGGTATCAAGCCAGCAAACCGGATGCACTCTTCAAGTTG
GAACAAGGAGAACAACTGTGGACAATTGAAGATGGAATCCACAGTGGAGCCTGTTCAGAC
ATATGGAAAGTTGATCATGTGCTGGAGCGCTTGCAGAGTGAAAGCCTGGTGAACAGAAGG
AAACCATGTCATGAACATGATGCATTTGAAAATATTGTTCATTGCAGCAAAAGTCAGTTT
CTGTTAGGGCAAAATCATGATATATTTGACTTACGTGGAAAAAGTTTGAAATCCAATTTA
ACTTTAGTTAACCAGAGCAAAGGCTATGAAATAAAGAACTCTGTTGAGTTTACTGGAAAT
GGGGACTCCTTTCTTCATGCTAACCATGAACGACTTCATACTGCAATTAAATTCCCTGCA
AGTCAAAAACTCATCAGCACTAAGTCCCAATTCATCAGTCCCAAGCATCAGAAAACACGA
AAATTAGAGAAGCATCATGTGTGCAGTGAATGTGGGAAAGCCTTCATCAAGAAGTCTTGG
CTAACTGATCACCAGGTAATGCATACAGGAGAGAAACCCCACAGATGTAGTCTATGTGAG
AAAGCCTTCTCCAGAAAGTTCATGCTTACTGAACATCAGCGAACTCATACAGGAGAAAAA
CCTTATGAATGCCCTGAATGTGGCAAAGCCTTTCTCAAGAAATCACGGCTCAACATACAT
CAGAAAACACATACCGGAGAGAAACCCTATATATGCAGTGAATGTGGAAAAGGCTTCATC
CAGAAAGGAAATCTCATTGTACACCAGCGAATTCATACAGGTGAGAAACCTTATATATGC
AATGAATGTGGAAAAGGCTTCATTCAGAAGACGTGTCTCATAGCACATCAGAGATTTCAC
ACAGGAAAGACGCCCTTTGTGTGCAGTGAATGTGGAAAATCCTGTTCTCAGAAATCAGGT
CTCATTAAACATCAAAGAATTCACACAGGAGAGAAACCCTTTGAATGTAGTGAATGTGGG
AAAGCCTTTAGCACAAAGCAAAAGCTCATTGTCCATCAAAGGACTCATACAGGAGAGAGA
CCCTATGGCTGTAACGAGTGTGGGAAAGCGTTTGCGTATATGTCGTGTCTGGTTAAGCAT
AAGAGAATACACACAAGGGAGAAACAAGAGGCAGCCAAGGTGGAAAATCCTCCTGCAGAG
AGGCACAGCTCATTACACACCAGTGATGTCATGCAGGAGAAAAACTCTGCTAACGGGGCG
ACTACACAAGTGCCTTCTGTGGCCCCTCAGACATCATTAAACATCAGCGGCCTCCTCGCA
AACAGGAACGTAGTCCTTGTGGGACAGCCAGTGGTCAGATGTGCAGCCTCAGGAGATAAC
AGAGGATTTGCACAGGACAGAAACCTTGTGAATGCAGTGAATGTGGTTGTGCCTTCCGTG
ATCAATTATGTCTTATTTTATGTTACAGAAAACCCATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1776
Start on reference sequence 178
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZNF350
Symbol Nomenclature : ZNF350
SYNONYM : ZBRK1; ZFQR
Designation : KRAB zinc finger protein ZFQR|zinc finger and BRCA1-interacting protein with a KRAB domain 1|zinc finger protein ZBRK1|zinc-finger protein ZBRK1
Full Nomenclature : zinc finger protein 350
GENEID : 59348
HGNC : 16656
MIM : 605422
Vega : OTTHUMG00000182538
Target GenBank: BC009921

 

Reference Sequence Alignment

  

HsCD00040462      ATGATCCAGGCCCAGGAATCCATAACACTGGAGGATGTGGCTGTGGACTTCACTTGGGAG
NM_021632.3       ATGATCCAGGCCCAGGAATCCATAACACTGGAGGATGTGGCTGTGGACTTCACTTGGGAG
                  ************************************************************

HsCD00040462      GAGTGGCAACTCCTGGGCGCTGCTCAGAAGGACCTGTACCGGGACGTGATGTTGGAGAAC
NM_021632.3       GAGTGGCAACTCCTGGGCGCTGCTCAGAAGGACCTGTACCGGGATGTGATGTTGGAGAAC
                  ******************************************** ***************

HsCD00040462      TACAGCAACCTGGTGGCAGTGGGGTATCAAGCCAGCAAACCGGATGCACTCTTCAAGTTG
NM_021632.3       TACAGCAACCTGGTGGCAGTGGGGTATCAAGCCAGCAAACCGGATGCACTCTTCAAGTTG
                  ************************************************************

HsCD00040462      GAACAAGGAGAACAACTGTGGACAATTGAAGATGGAATCCACAGTGGAGCCTGTTCAGAC
NM_021632.3       GAACAAGGAGAACAACTGTGGACAATTGAAGATGGAATCCACAGTGGAGCCTGTTCAGAC
                  ************************************************************

HsCD00040462      ATATGGAAAGTTGATCATGTGCTGGAGCGCTTGCAGAGTGAAAGCCTGGTGAACAGAAGG
NM_021632.3       ATATGGAAAGTTGATCATGTGCTGGAGCGCTTGCAGAGTGAAAGCCTGGTGAACAGAAGG
                  ************************************************************

HsCD00040462      AAACCATGTCATGAACATGATGCATTTGAAAATATTGTTCATTGCAGCAAAAGTCAGTTT
NM_021632.3       AAACCATGTCATGAACATGATGCATTTGAAAATATTGTTCATTGCAGCAAAAGTCAGTTT
                  ************************************************************

HsCD00040462      CTGTTAGGGCAAAATCATGATATATTTGACTTACGTGGAAAAAGTTTGAAATCCAATTTA
NM_021632.3       CTGTTAGGGCAAAATCATGATATATTTGACTTACGTGGAAAAAGTTTGAAATCCAATTTA
                  ************************************************************

HsCD00040462      ACTTTAGTTAACCAGAGCAAAGGCTATGAAATAAAGAACTCTGTTGAGTTTACTGGAAAT
NM_021632.3       ACTTTAGTTAACCAGAGCAAAGGCTATGAAATAAAGAACTCTGTTGAGTTTACTGGAAAT
                  ************************************************************

HsCD00040462      GGGGACTCCTTTCTTCATGCTAACCATGAACGACTTCATACTGCAATTAAATTCCCTGCA
NM_021632.3       GGGGACTCCTTTCTTCATGCTAACCATGAACGACTTCATACTGCAATTAAATTCCCTGCA
                  ************************************************************

HsCD00040462      AGTCAAAAACTCATCAGCACTAAGTCCCAATTCATCAGTCCCAAGCATCAGAAAACACGA
NM_021632.3       AGTCAAAAACTCATCAGCACTAAGTCCCAATTCATCAGTCCCAAGCATCAGAAAACACGA
                  ************************************************************

HsCD00040462      AAATTAGAGAAGCATCATGTGTGCAGTGAATGTGGGAAAGCCTTCATCAAGAAGTCTTGG
NM_021632.3       AAATTAGAGAAGCATCATGTGTGCAGTGAATGTGGGAAAGCCTTCATCAAGAAGTCTTGG
                  ************************************************************

HsCD00040462      CTAACTGATCACCAGGTAATGCATACAGGAGAGAAACCCCACAGATGTAGTCTATGTGAG
NM_021632.3       CTAACTGATCACCAGGTAATGCATACAGGAGAGAAACCCCACAGATGTAGTCTATGTGAG
                  ************************************************************

HsCD00040462      AAAGCCTTCTCCAGAAAGTTCATGCTTACTGAACATCAGCGAACTCATACAGGAGAAAAA
NM_021632.3       AAAGCCTTCTCCAGAAAGTTCATGCTTACTGAACATCAGCGAACTCATACAGGAGAAAAA
                  ************************************************************

HsCD00040462      CCTTATGAATGCCCTGAATGTGGCAAAGCCTTTCTCAAGAAATCACGGCTCAACATACAT
NM_021632.3       CCTTATGAATGCCCTGAATGTGGCAAAGCCTTTCTCAAGAAATCACGGCTCAACATACAT
                  ************************************************************

HsCD00040462      CAGAAAACACATACCGGAGAGAAACCCTATATATGCAGTGAATGTGGAAAAGGCTTCATC
NM_021632.3       CAGAAAACACATACCGGAGAGAAACCCTATATATGCAGTGAATGTGGAAAAGGCTTCATC
                  ************************************************************

HsCD00040462      CAGAAAGGAAATCTCATTGTACACCAGCGAATTCATACAGGTGAGAAACCTTATATATGC
NM_021632.3       CAGAAAGGAAATCTCATTGTACACCAGCGAATTCATACAGGTGAGAAACCTTATATATGC
                  ************************************************************

HsCD00040462      AATGAATGTGGAAAAGGCTTCATTCAGAAGACGTGTCTCATAGCACATCAGAGATTTCAC
NM_021632.3       AATGAATGTGGAAAAGGCTTCATTCAGAAGACGTGTCTCATAGCACATCAGAGATTTCAC
                  ************************************************************

HsCD00040462      ACAGGAAAGACGCCCTTTGTGTGCAGTGAATGTGGAAAATCCTGTTCTCAGAAATCAGGT
NM_021632.3       ACAGGAAAGACGCCCTTTGTGTGCAGTGAATGTGGAAAATCCTGTTCTCAGAAATCAGGT
                  ************************************************************

HsCD00040462      CTCATTAAACATCAAAGAATTCACACAGGAGAGAAACCCTTTGAATGTAGTGAATGTGGG
NM_021632.3       CTCATTAAACATCAAAGAATTCACACAGGAGAGAAACCCTTTGAATGTAGTGAATGTGGG
                  ************************************************************

HsCD00040462      AAAGCCTTTAGCACAAAGCAAAAGCTCATTGTCCATCAAAGGACTCATACAGGAGAGAGA
NM_021632.3       AAAGCCTTTAGCACAAAGCAAAAGCTCATTGTCCATCAAAGGACTCATACAGGAGAGAGA
                  ************************************************************

HsCD00040462      CCCTATGGCTGTAACGAGTGTGGGAAAGCGTTTGCGTATATGTCGTGTCTGGTTAAGCAT
NM_021632.3       CCCTATGGCTGTAACGAGTGTGGGAAAGCGTTTGCGTATATGTCGTGTCTGGTTAAGCAT
                  ************************************************************

HsCD00040462      AAGAGAATACACACAAGGGAGAAACAAGAGGCAGCCAAGGTGGAAAATCCTCCTGCAGAG
NM_021632.3       AAGAGAATACACACAAGGGAGAAACAAGAGGCAGCCAAGGTGGAAAATCCTCCTGCAGAG
                  ************************************************************

HsCD00040462      AGGCACAGCTCATTACACACCAGTGATGTCATGCAGGAGAAAAACTCTGCTAACGGGGCG
NM_021632.3       AGGCACAGCTCATTACACACCAGTGATGTCATGCAGGAGAAAAACTCTGCTAACGGGGCG
                  ************************************************************

HsCD00040462      ACTACACAAGTGCCTTCTGTGGCCCCTCAGACATCATTAAACATCAGCGGCCTCCTCGCA
NM_021632.3       ACTACACAAGTGCCTTCTGTGGCCCCTCAGACATCATTAAACATCAGCGGCCTCCTCGCA
                  ************************************************************

HsCD00040462      AACAGGAACGTAGTCCTTGTGGGACAGCCAGTGGTCAGATGTGCAGCCTCAGGAGATAAC
NM_021632.3       AACAGGAACGTAGTCCTTGTGGGACAGCCAGTGGTCAGATGTGCAGCCTCAGGAGATAAC
                  ************************************************************

HsCD00040462      AGAGGATTTGCACAGGACAGAAACCTTGTGAATGCAGTGAATGTGGTTGTGCCTTCCGTG
NM_021632.3       AGAGGATTTGCACAGGACAGAAACCTTGTGAATGCAGTGAATGTGGTTGTGCCTTCCGTG
                  ************************************************************

HsCD00040462      ATCAATTATGTCTTATTTTATGTTACAGAAAACCCATTG
NM_021632.3       ATCAATTATGTCTTATTTTATGTTACAGAAAACCCATAG
                  *************************************:*

Pathways

Reactome : Gene Expression
Reactome : Generic Transcription Pathway

Protein

SMART domain : KRAB : krueppel associated box
SMART domain : ZnF_C2H2 : zinc finger
UniProt : Q9GZX5
HPRD : 05663

Clone

Cloning Information : 193338
HIP Master Clone ID : 168049
Original Clone ID : FLH193338.01L

Organism


TAX_ID : 9606
Species Specific ID: 59348

Genome

Chromosome : 19
Map Location : 19q13.41
Ensembl : ENSG00000256683

Reagents

Labome : ZBRK1-antibody