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SRP54 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  SRP54
Gene Name:  signal recognition particle 54kDa
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100073933)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404869

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1512nts         Open reading frame : 51 to 1562
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTTCTAG
CAGACCTTGGAAGAAAAATAACATCAGCATTACGCTCATTGAGCAATGCCACCATTATCA
ATGAAGAGGTATTGAATGCTATGCTAAAAGAAGTCTGTACCGCTTTGTTGGAAGCAGATG
TTAATATTAAACTAGTGAAGCAACTAAGAGAAAATGTTAAGTCTGCTATTGATCTTGAAG
AGATGGCATCTGGTCTTAACAAAAGAAAAATGATTCAGCATGCTGTATTTAAAGAACTTG
TGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACCCACTAAAGGAAAACAAAATGTGA
TTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCAAAGCTAGCATATT
ATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGAGCAGGGG
CTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATA
CAGAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATT
TTGAAATTATTATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAG
AAATGCTTCAAGTTGCTAATGCTATACAACCTGATAACATTGTTTATGTGATGGATGCCT
CCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTTTAAAGATAAAGTAGATGTAGCCT
CAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCACTCAGTGCAGTCG
CTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGATGACTTTGAAC
CTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGACTGA
TAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAAC
ATGGTCAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCC
CCTTCAGTCAGATCTTGGGGATGATCCCTGGTTTTGGGACAGATTTTATGAGCAAAGGAA
ATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAATGACAATAATGGATAGTATGAATG
ATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAACCAGGAAGAATCC
AAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTTTTGACACAAT
ATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGTG
GCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCCA
AAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGA
TGAGGCAGTTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGATTCAATAATA
TGTACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRP54
Symbol Nomenclature : SRP54
Designation : signal recognition particle 54 kDa protein
Full Nomenclature : signal recognition particle 54kDa
GENEID : 6729
GenBank Accession : JF432694
HGNC : 11301
MIM : 604857
Vega : OTTHUMG00000140214
Target GenBank: JF432694

 

Reference Sequence Alignment

  

HsCD00404869      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGTTCTAG
NM_003136.3       --------------------------------------------------ATGGTTCTAG
                                                                    **********

HsCD00404869      CAGACCTTGGAAGAAAAATAACATCAGCATTACGCTCATTGAGCAATGCCACCATTATCA
NM_003136.3       CAGACCTTGGAAGAAAAATAACATCAGCATTACGCTCGTTGAGCAATGCCACCATTATCA
                  *************************************.**********************

HsCD00404869      ATGAAGAGGTATTGAATGCTATGCTAAAAGAAGTCTGTACCGCTTTGTTGGAAGCAGATG
NM_003136.3       ATGAAGAGGTATTGAATGCTATGCTAAAAGAAGTCTGTACCGCTTTGTTGGAAGCAGATG
                  ************************************************************

HsCD00404869      TTAATATTAAACTAGTGAAGCAACTAAGAGAAAATGTTAAGTCTGCTATTGATCTTGAAG
NM_003136.3       TTAATATTAAACTAGTGAAGCAACTAAGAGAAAATGTTAAGTCTGCTATTGATCTTGAAG
                  ************************************************************

HsCD00404869      AGATGGCATCTGGTCTTAACAAAAGAAAAATGATTCAGCATGCTGTATTTAAAGAACTTG
NM_003136.3       AGATGGCATCTGGTCTTAACAAAAGAAAAATGATTCAGCATGCTGTATTTAAAGAACTTG
                  ************************************************************

HsCD00404869      TGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACCCACTAAAGGAAAACAAAATGTGA
NM_003136.3       TGAAGCTTGTAGACCCTGGAGTTAAGGCATGGACACCCACTAAAGGAAAACAAAATGTGA
                  ************************************************************

HsCD00404869      TTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCAAAGCTAGCATATT
NM_003136.3       TTATGTTTGTTGGATTGCAAGGGAGTGGTAAAACAACAACATGTTCAAAGCTAGCATATT
                  ************************************************************

HsCD00404869      ATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGAGCAGGGG
NM_003136.3       ATTACCAGAGGAAAGGTTGGAAGACCTGTTTAATATGTGCAGACACATTCAGAGCAGGGG
                  ************************************************************

HsCD00404869      CTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATA
NM_003136.3       CTTTTGACCAACTAAAACAGAATGCTACCAAAGCAAGAATTCCATTTTATGGAAGCTATA
                  ************************************************************

HsCD00404869      CAGAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATT
NM_003136.3       CAGAAATGGATCCTGTCATCATTGCTTCTGAAGGAGTAGAGAAATTTAAAAATGAAAATT
                  ************************************************************

HsCD00404869      TTGAAATTATTATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAG
NM_003136.3       TTGAAATTATTATTGTTGATACAAGTGGCCGCCACAAACAAGAAGACTCTTTGTTTGAAG
                  ************************************************************

HsCD00404869      AAATGCTTCAAGTTGCTAATGCTATACAACCTGATAACATTGTTTATGTGATGGATGCCT
NM_003136.3       AAATGCTTCAAGTTGCTAATGCTATACAACCTGATAACATTGTTTATGTGATGGATGCCT
                  ************************************************************

HsCD00404869      CCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTTTAAAGATAAAGTAGATGTAGCCT
NM_003136.3       CCATTGGGCAGGCTTGTGAAGCCCAGGCTAAGGCTTTTAAAGATAAAGTAGATGTAGCCT
                  ************************************************************

HsCD00404869      CAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCACTCAGTGCAGTCG
NM_003136.3       CAGTAATAGTGACAAAACTTGATGGCCATGCAAAAGGAGGTGGTGCACTCAGTGCAGTCG
                  ************************************************************

HsCD00404869      CTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGATGACTTTGAAC
NM_003136.3       CTGCCACAAAAAGTCCGATTATTTTCATTGGTACAGGGGAACATATAGATGACTTTGAAC
                  ************************************************************

HsCD00404869      CTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGACTGA
NM_003136.3       CTTTCAAAACACAGCCTTTTATTAGCAAACTTCTTGGTATGGGCGACATTGAAGGACTGA
                  ************************************************************

HsCD00404869      TAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAAC
NM_003136.3       TAGATAAAGTCAACGAGTTGAAGTTGGATGACAATGAAGCACTTATAGAGAAGTTGAAAC
                  ************************************************************

HsCD00404869      ATGGTCAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCC
NM_003136.3       ATGGTCAGTTTACGTTGCGAGACATGTATGAGCAATTTCAAAATATCATGAAAATGGGCC
                  ************************************************************

HsCD00404869      CCTTCAGTCAGATCTTGGGGATGATCCCTGGTTTTGGGACAGATTTTATGAGCAAAGGAA
NM_003136.3       CCTTCAGTCAGATCTTGGGGATGATCCCTGGTTTTGGGACAGATTTTATGAGCAAAGGAA
                  ************************************************************

HsCD00404869      ATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAATGACAATAATGGATAGTATGAATG
NM_003136.3       ATGAACAGGAGTCAATGGCAAGGCTAAAGAAATTAATGACAATAATGGATAGTATGAATG
                  ************************************************************

HsCD00404869      ATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAACCAGGAAGAATCC
NM_003136.3       ATCAAGAACTAGACAGTACGGATGGTGCCAAAGTTTTTAGTAAACAACCAGGAAGAATCC
                  ************************************************************

HsCD00404869      AAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTTTTGACACAAT
NM_003136.3       AAAGAGTAGCAAGAGGATCGGGTGTATCAACAAGAGATGTTCAAGAACTTTTGACACAAT
                  ************************************************************

HsCD00404869      ATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGTG
NM_003136.3       ATACCAAGTTTGCACAGATGGTAAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGTG
                  ************************************************************

HsCD00404869      GCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCCA
NM_003136.3       GCGACATGTCTAAGAATGTGAGCCAGTCACAGATGGCAAAATTGAACCAACAAATGGCCA
                  ************************************************************

HsCD00404869      AAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGA
NM_003136.3       AAATGATGGATCCTAGGGTTCTTCATCACATGGGTGGTATGGCAGGACTTCAGTCAATGA
                  ************************************************************

HsCD00404869      TGAGGCAGTTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGATTCAATAATA
NM_003136.3       TGAGGCAGTTTCAACAGGGTGCTGCTGGCAACATGAAAGGCATGATGGGATTCAATAATA
                  ************************************************************

HsCD00404869      TGTACCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAA
NM_003136.3       TGTAA-------------------------------------------------
                  ****.                                                 

Pathways

Reactome : Gene Expression
Reactome : Metabolism of proteins
Reactome : SRP-dependent cotranslational protein targeting to membrane
Reactome : Translation
WikiPathway : mRNA processing

Clone

Cloning Information : 580309

Organism


TAX_ID : 9606
Species Specific ID: 6729

Genome

Chromosome : 14
Map Location : 14q13.2
Ensembl : ENSG00000100883

Reagents

Labome : SRP54-antibody