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CAPN7 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN7
Gene Name:  calpain 7
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: generated from ORFeome entry clone (IMAGE:100074151)
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00404981

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2439nts         Open reading frame : 51 to 2489
Reference Sequence Alignment

TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGACGCCA
CAGCACTGGAACGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCGC
TAGACAGAGCAGAAGCGCTGGGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
CAAGTGTTAAGCCAAAGCCACCTCCAGAGAGAGCACATTTTCCACTGGGCGCTAATCCCT
TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
GTTGGAAAGGAAGATACAGTGAAAATGATGTAGAAAACTGGACCCCAGAGTTGCAAAAGT
ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
TTATCCCCATCAAGATCACACAACTTCAGTGCCCAACTTTCTTGTACAAAGTTGGCATTA
TAAGAAAGCATTGCTTATCAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN7
Symbol Nomenclature : CAPN7
SYNONYM : CALPAIN7; PALBH
Designation : calpain like protease|calpain-7|homolog of Aspergillus Nidulans PALB|palB homolog
Full Nomenclature : calpain 7
GENEID : 23473
GenBank Accession : JF432832
HGNC : 1484
MIM : 606400
Vega : OTTHUMG00000129863
Target GenBank: JF432832

 

Reference Sequence Alignment

  

HsCD00404981      TCCATTAGGGATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGGCATGGACGCCA
NM_014296.2       --------------------------------------------------ATGGACGCCA
                                                                    **********

HsCD00404981      CAGCACTGGAACGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
NM_014296.2       CAGCACTGGAGCGGGACGCTGTGCAGTTCGCCCGTCTGGCGGTTCAGCGCGACCACGAAG
                  **********.*************************************************

HsCD00404981      GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
NM_014296.2       GCCGCTACTCCGAGGCGGTGTTTTATTACAAGGAAGCTGCACAAGCCTTAATTTATGCTG
                  ************************************************************

HsCD00404981      AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
NM_014296.2       AGATGGCAGGATCAAGCCTAGAAAATATTCAAGAAAAAATAACTGAGTATCTGGAAAGAG
                  ************************************************************

HsCD00404981      TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
NM_014296.2       TTCAAGCTCTACATTCAGCAGTTCAGTCAAAGAGTGCTGATCCTTTGAAGTCAAAACATC
                  ************************************************************

HsCD00404981      AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
NM_014296.2       AGTTGGACTTAGAGCGTGCTCATTTCCTTGTTACACAAGCTTTTGATGAAGATGAAAAAG
                  ************************************************************

HsCD00404981      AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
NM_014296.2       AGAATGTTGAAGATGCTATAGAATTGTACACAGAAGCTGTGGATCTCTGTCTGAAAACAT
                  ************************************************************

HsCD00404981      CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCGC
NM_014296.2       CTTATGAAACTGCTGATAAAGTCCTGCAAAATAAACTGAAACAGTTGGCTCGACAGGCAC
                  **********************************************************.*

HsCD00404981      TAGACAGAGCAGAAGCGCTGGGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
NM_014296.2       TAGACAGAGCAGAAGCGCTGAGTGAGCCTTTGACCAAGCCAGTTGGCAAAATCAGTTCAA
                  ********************.***************************************

HsCD00404981      CAAGTGTTAAGCCAAAGCCACCTCCAGAGAGAGCACATTTTCCACTGGGCGCTAATCCCT
NM_014296.2       CAAGTGTTAAGCCAAAGCCACCTCCAGTGAGAGCACATTTTCCACTGGGCGCTAATCCCT
                  ***************************:********************************

HsCD00404981      TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
NM_014296.2       TCCTTGAAAGACCTCAGTCATTTATAAGTCCTCAGTCATGTGATGCACAAGGACAGAGAT
                  ************************************************************

HsCD00404981      ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
NM_014296.2       ACACAGCAGAAGAAATAGAAGTACTCAGGACAACATCAAAAATAAATGGTATAGAATATG
                  ************************************************************

HsCD00404981      TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
NM_014296.2       TTCCTTTCATGAATGTTGACCTGAGAGAACGTTTTGCCTATCCAATGCCTTTCTGTGATA
                  ************************************************************

HsCD00404981      GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
NM_014296.2       GATGGGGCAAGCTACCATTATCACCTAAACAAAAAACTACATTTTCCAAGTGGGTACGAC
                  ************************************************************

HsCD00404981      CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
NM_014296.2       CAGAAGACCTCACCAACAATCCTACAATGATATATACTGTGTCCAGTTTTAGCATAAAGC
                  ************************************************************

HsCD00404981      AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
NM_014296.2       AGACAATAGTATCGGATTGCTCCTTTGTGGCATCACTGGCCATCAGTGCAGCTTATGAAA
                  ************************************************************

HsCD00404981      GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
NM_014296.2       GACGTTTTAATAAGAAGTTAATTACCGGCATAATTTACCCTCAAAACAAGGATGGTGAAC
                  ************************************************************

HsCD00404981      CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
NM_014296.2       CAGAATACAATCCATGTGGGAAGTATATGGTAAAACTTCACCTCAATGGTGTCCCAAGAA
                  ************************************************************

HsCD00404981      AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
NM_014296.2       AGGTGATAATTGATGACCAGTTACCTGTTGATCACAAGGGAGAATTGCTCTGTTCTTATT
                  ************************************************************

HsCD00404981      CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
NM_014296.2       CCAACAACAAAAGTGAATTATGGGTTTCTCTCATAGAAAAAGCATACATGAAAGTCATGG
                  ************************************************************

HsCD00404981      GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
NM_014296.2       GAGGATATGATTTTCCAGGATCCAACTCCAATATTGATCTTCATGCACTGACTGGCTGGA
                  ************************************************************

HsCD00404981      TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
NM_014296.2       TACCAGAAAGAATTGCTATGCATTCAGATAGCCAAACTTTCAGTAAGGATAATTCTTTCA
                  ************************************************************

HsCD00404981      GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
NM_014296.2       GAATGCTTTATCAAAGATTTCACAAAGGAGATGTCCTCATCACTGCGTCAACTGGAATGA
                  ************************************************************

HsCD00404981      TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
NM_014296.2       TGACAGAAGCTGAAGGAGAGAAGTGGGGTCTGGTTCCCACACACGCATATGCTGTTTTGG
                  ************************************************************

HsCD00404981      ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
NM_014296.2       ATATTAGAGAGTTCAAGGGGCTGCGATTTATCCAGTTGAAAAATCCTTGGAGTCATTTAC
                  ************************************************************

HsCD00404981      GTTGGAAAGGAAGATACAGTGAAAATGATGTAGAAAACTGGACCCCAGAGTTGCAAAAGT
NM_014296.2       GTTGGAAAGGAAGATACAGTGAAAATGATGTAAAAAACTGGACTCCAGAGTTGCAAAAGT
                  ********************************.********** ****************

HsCD00404981      ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
NM_014296.2       ATTTAAACTTTGATCCCCGAACAGCTCAGAAAATAGACAACGGAATATTTTGGATTTCCT
                  ************************************************************

HsCD00404981      GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
NM_014296.2       GGGATGATCTCTGCCAGTATTATGATGTGATTTATTTGAGTTGGAATCCAGGTCTTTTTA
                  ************************************************************

HsCD00404981      AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
NM_014296.2       AAGAATCAACATGTATTCACAGTACTTGGGATGCTAAGCAAGGACCTGTGAAAGATGCCT
                  ************************************************************

HsCD00404981      ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
NM_014296.2       ATAGCCTGGCCAACAACCCCCAGTACAAACTGGAGGTGCAGTGTCCACAGGGGGGTGCTG
                  ************************************************************

HsCD00404981      CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
NM_014296.2       CAGTTTGGGTTTTGCTTAGTAGACACATAACAGACAAGGATGATTTTGCGAATAATCGAG
                  ************************************************************

HsCD00404981      AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
NM_014296.2       AATTTATCACAATGGTTGTATACAAGACTGATGGGAAAAAAGTTTATTACCCAGCTGACC
                  ************************************************************

HsCD00404981      CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
NM_014296.2       CACCTCCATACATTGATGGAATTCGAATTAACAGCCCTCATTATTTGACTAAGATAAAGC
                  ************************************************************

HsCD00404981      TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
NM_014296.2       TGACCACACCTGGCACCCATACCTTTACATTAGTGGTTTCTCAATATGAAAAACAGAACA
                  ************************************************************

HsCD00404981      CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
NM_014296.2       CAATCCATTACACGGTTCGGGTATATTCAGCATGCAGCTTTACTTTTTCAAAGATTCCTT
                  ************************************************************

HsCD00404981      CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
NM_014296.2       CACCATACACCTTATCAAAACGGATTAATGGAAAGTGGAGTGGTCAGAGTGCTGGAGGAT
                  ************************************************************

HsCD00404981      GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
NM_014296.2       GTGGAAATTTCCAAGAGACTCACAAAAATAACCCCATCTACCAATTCCATATAGAAAAGA
                  ************************************************************

HsCD00404981      CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
NM_014296.2       CTGGGCCGTTACTGATTGAGCTACGAGGACCAAGGCAATATAGCGTTGGATTTGAGGTTG
                  ************************************************************

HsCD00404981      TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
NM_014296.2       TAACAGTTTCTACTCTAGGAGATCCTGGTCCCCATGGCTTTCTGAGGAAATCTAGTGGTG
                  ************************************************************

HsCD00404981      ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
NM_014296.2       ACTATAGGTGTGGGTTTTGCTACCTGGAATTAGAAAATATACCTTCTGGGATCTTCAATA
                  ************************************************************

HsCD00404981      TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
NM_014296.2       TCATTCCTAGTACCTTTTTGCCTAAACAAGAAGGACCTTTTTTCTTGGACTTTAATAGTA
                  ************************************************************

HsCD00404981      TTATCCCCATCAAGATCACACAACTTCAGTGCCCAACTTTCTTGTACAAAGTTGGCATTA
NM_014296.2       TTATCCCCATCAAGATCACACAACTTCAGTGA----------------------------
                  *******************************.                            

HsCD00404981      TAAGAAAGCATTGCTTATCAA
NM_014296.2       ---------------------
                                       

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 581594

Organism


TAX_ID : 9606
Species Specific ID: 23473

Genome

Chromosome : 3
Map Location : 3p24
Ensembl : ENSG00000131375

Reagents

Labome : CAPN7-antibody