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PPIL2 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPIL2
Gene Name:  peptidylprolyl isomerase (cyclophilin)-like 2
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH179043.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040620

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1563nts         Open reading frame : 1 to 1563
Reference Sequence Alignment

ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGATAGCCCCGGAGACCAAA
GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
TTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1628
Start on reference sequence 66
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPIL2
Symbol Nomenclature : PPIL2
SYNONYM : CYC4; CYP60; Cyp-60; UBOX7; hCyP-60
Designation : PPIase|U-box domain containing 7|cyclophilin, 60kDa|cyclophilin-60|cyclophilin-like protein CyP-60|peptidyl-prolyl cis-trans isomerase-like 2|peptidylprolyl cis-trans isomerase|rotamase PPIL2
Full Nomenclature : peptidylprolyl isomerase (cyclophilin)-like 2
GENEID : 23759
HGNC : 9261
MIM : 607588
Vega : OTTHUMG00000030174
Target GenBank: BC028385

 

Reference Sequence Alignment

  

HsCD00040620      ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_148175.2       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_148176.2       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_014337.3       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
                  ************************************************************

HsCD00040620      TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_148175.2       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_148176.2       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_014337.3       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
                  ************************************************************

HsCD00040620      CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_148175.2       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_148176.2       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_014337.3       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
                  ************************************************************

HsCD00040620      TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_148175.2       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_148176.2       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_014337.3       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
                  ************************************************************

HsCD00040620      GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_148175.2       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_148176.2       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_014337.3       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
                  ************************************************************

HsCD00040620      TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_148175.2       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_148176.2       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_014337.3       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
                  ************************************************************

HsCD00040620      ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_148175.2       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_148176.2       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_014337.3       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
                  ************************************************************

HsCD00040620      TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_148175.2       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_148176.2       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_014337.3       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
                  ************************************************************

HsCD00040620      CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_148175.2       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_148176.2       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_014337.3       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
                  ************************************************************

HsCD00040620      ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_148175.2       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_148176.2       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_014337.3       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
                  ************************************************************

HsCD00040620      AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_148175.2       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_148176.2       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_014337.3       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
                  ************************************************************

HsCD00040620      CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
NM_148175.2       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
NM_148176.2       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
NM_014337.3       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
                  ************************************************************

HsCD00040620      TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_148175.2       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_148176.2       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_014337.3       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
                  ************************************************************

HsCD00040620      CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_148175.2       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_148176.2       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_014337.3       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
                  ************************************************************

HsCD00040620      TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_148175.2       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_148176.2       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_014337.3       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
                  ************************************************************

HsCD00040620      CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_148175.2       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_148176.2       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_014337.3       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
                  ************************************************************

HsCD00040620      TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_148175.2       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_148176.2       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_014337.3       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
                  ************************************************************

HsCD00040620      GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_148175.2       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_148176.2       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_014337.3       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
                  ************************************************************

HsCD00040620      ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_148175.2       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_148176.2       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_014337.3       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
                  ************************************************************

HsCD00040620      TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_148175.2       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_148176.2       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_014337.3       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
                  ************************************************************

HsCD00040620      GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_148175.2       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_148176.2       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_014337.3       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
                  ************************************************************

HsCD00040620      CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_148175.2       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_148176.2       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_014337.3       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
                  ************************************************************

HsCD00040620      GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGATAGCCCCGGAGACCAAA
NM_148175.2       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
NM_148176.2       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
NM_014337.3       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
                  ******************************************.*****************

HsCD00040620      GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_148175.2       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_148176.2       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_014337.3       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
                  ************************************************************

HsCD00040620      GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
NM_148175.2       GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
NM_148176.2       GGCAAGTACATCAACCCAGCAGCCACCGAGCAG----CAGAGGAAGAGCCCTCAACCAGT
NM_014337.3       GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
                  ************************** .***..    ***********************

HsCD00040620      GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_148175.2       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_148176.2       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_014337.3       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
                  ************************************************************

HsCD00040620      TTG-------------------------
NM_148175.2       TAG-------------------------
NM_148176.2       TAGCAGCAGGCTGCCTGATGACCACTAG
NM_014337.3       TAG-------------------------
                  *:*                         

Pathways

Reactome : Basigin interactions
Reactome : Cell surface interactions at the vascular wall
Reactome : Hemostasis

Protein

SMART domain : Ubox : Modified RING finger domain
UniProt : Q13356
HPRD : 08472

Clone

Cloning Information : 179043
HIP Master Clone ID : 154144
Original Clone ID : FLH179043.01L

Organism


TAX_ID : 9606
Species Specific ID: 23759

Genome

Chromosome : 22
Map Location : 22q11.21
Ensembl : ENSG00000100023

Reagents

Labome : PPIL2-antibody