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ESAM (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ESAM
Gene Name:  endothelial cell adhesion molecule
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH179749.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040726

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1173nts         Open reading frame : 1 to 1173
Reference Sequence Alignment

ATGATTTCCCTCCCGGGGCCCCTGGTGACCAACTTGCTGCGGTTTTTGTTCCTGGGGCTG
AGTGCCCTCGCGCCCCCCTCGCGGGCCCAGCTGCAACTGCACTTGCCCGCCAACCGGTTG
CAGGCGGTGGAGGGAGGGGAAGTGGTGCTTCCAGCGTGGTACACCTTGCACGGGGAGGTG
TCTTCATCCCAGCCATGGGAGGTGCCCTTTGTGATGTGGTTCTTCAAACAGAAAGAAAAG
GAGGATCAGGTGTTGTCCTACATCAATGGGGTCACAACAAGCAAACCTGGAGTATCCTTG
GTCTACTCCATGCCCTCCCGGAACCTGTCCCTGCGGCTGGAGGGTCTCCAGGAGAAAGAC
TCTGGCCCCTACAGCTGCTCCGTGAATGTGCAAGACAAACAAGGCAAATCTAGGGGCCAC
AGCATCAAAACCTTAGAACTCAATGTACTGGTTCCTCCAGCTCCTCCATCCTGCCGTCTC
CAGGGTGTGCCCCATGTGGGGGCAAACGTGACCCTGAGCTGCCAGTCTCCAAGGAGTAAG
CCCGCTGTCCAATACCAGTGGGATCGGCAGCTTCCATCCTTCCAGACTTTCTTTGCACCA
GCATTAGATGTCATCCGTGGGTCTTTAAGCCTCACCAACCTTTCGTCTTCCATGGCTGGA
GTCTATGTCTGCAAGGCCCACAATGAGGTGGGCACTGCCCAATGTAATGTGACGCTGGAA
GTGAGCACAGGGCCTGGAGCTGCAGTGGTTGCTGGAGCTGTTGTGGGTACCCTGGTTGGA
CTGGGGTTGCTGGCTGGGCTGGTCCTCTTGTACCACCGCCGGGGCAAGGCCCTGGAGGAG
CCAGCCAATGATATCAAGGAGGATGCCATTGCTCCCCGGACCCTGCCCTGGCCCAAGAGC
TCAGACACAATCTCCAAGAATGGGACCCTTTCCTCTGTCACCTCCGCACGAGCCCTCCGG
CCACCCCATGGCCCTCCCAGGCCTGGTGCATTGACCCCCACGCCCAGTCTCTCCAGCCAG
GCCCTGCCCTCACCAAGACTGCCCACGACAGATGGGGCCCACCCTCAACCAATATCCCCC
ATCCCTGGTGGGGTTTCTTCCTCTGGCTTGAGCCGCATGGGTGCTGTGCCTGTGATGGTG
CCTGCCCAGAGTCAAGCTGGCTCTCTGGTATTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1180
Start on reference sequence 8
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ESAM
Symbol Nomenclature : ESAM
SYNONYM : W117m
Designation : 2310008D05Rik|HUEL (C4orf1)-interacting protein|LP4791 protein|endothelial cell-selective adhesion molecule
Full Nomenclature : endothelial cell adhesion molecule
GENEID : 90952
Locus Tag : UNQ220/PRO246
HGNC : 17474
MIM : 614281
Vega : OTTHUMG00000151986
Target GenBank: BC016868

 

Reference Sequence Alignment

  

HsCD00040726      ATGATTTCCCTCCCGGGGCCCCTGGTGACCAACTTGCTGCGGTTTTTGTTCCTGGGGCTG
NM_138961.2       ATGATTTCCCTCCCGGGGCCCCTGGTGACCAACTTGCTGCGGTTTTTGTTCCTGGGGCTG
                  ************************************************************

HsCD00040726      AGTGCCCTCGCGCCCCCCTCGCGGGCCCAGCTGCAACTGCACTTGCCCGCCAACCGGTTG
NM_138961.2       AGTGCCCTCGCGCCCCCCTCGCGGGCCCAGCTGCAACTGCACTTGCCCGCCAACCGGTTG
                  ************************************************************

HsCD00040726      CAGGCGGTGGAGGGAGGGGAAGTGGTGCTTCCAGCGTGGTACACCTTGCACGGGGAGGTG
NM_138961.2       CAGGCGGTGGAGGGAGGGGAAGTGGTGCTTCCAGCGTGGTACACCTTGCACGGGGAGGTG
                  ************************************************************

HsCD00040726      TCTTCATCCCAGCCATGGGAGGTGCCCTTTGTGATGTGGTTCTTCAAACAGAAAGAAAAG
NM_138961.2       TCTTCATCCCAGCCATGGGAGGTGCCCTTTGTGATGTGGTTCTTCAAACAGAAAGAAAAG
                  ************************************************************

HsCD00040726      GAGGATCAGGTGTTGTCCTACATCAATGGGGTCACAACAAGCAAACCTGGAGTATCCTTG
NM_138961.2       GAGGATCAGGTGTTGTCCTACATCAATGGGGTCACAACAAGCAAACCTGGAGTATCCTTG
                  ************************************************************

HsCD00040726      GTCTACTCCATGCCCTCCCGGAACCTGTCCCTGCGGCTGGAGGGTCTCCAGGAGAAAGAC
NM_138961.2       GTCTACTCCATGCCCTCCCGGAACCTGTCCCTGCGGCTGGAGGGTCTCCAGGAGAAAGAC
                  ************************************************************

HsCD00040726      TCTGGCCCCTACAGCTGCTCCGTGAATGTGCAAGACAAACAAGGCAAATCTAGGGGCCAC
NM_138961.2       TCTGGCCCCTACAGCTGCTCCGTGAATGTGCAAGACAAACAAGGCAAATCTAGGGGCCAC
                  ************************************************************

HsCD00040726      AGCATCAAAACCTTAGAACTCAATGTACTGGTTCCTCCAGCTCCTCCATCCTGCCGTCTC
NM_138961.2       AGCATCAAAACCTTAGAACTCAATGTACTGGTTCCTCCAGCTCCTCCATCCTGCCGTCTC
                  ************************************************************

HsCD00040726      CAGGGTGTGCCCCATGTGGGGGCAAACGTGACCCTGAGCTGCCAGTCTCCAAGGAGTAAG
NM_138961.2       CAGGGTGTGCCCCATGTGGGGGCAAACGTGACCCTGAGCTGCCAGTCTCCAAGGAGTAAG
                  ************************************************************

HsCD00040726      CCCGCTGTCCAATACCAGTGGGATCGGCAGCTTCCATCCTTCCAGACTTTCTTTGCACCA
NM_138961.2       CCCGCTGTCCAATACCAGTGGGATCGGCAGCTTCCATCCTTCCAGACTTTCTTTGCACCA
                  ************************************************************

HsCD00040726      GCATTAGATGTCATCCGTGGGTCTTTAAGCCTCACCAACCTTTCGTCTTCCATGGCTGGA
NM_138961.2       GCATTAGATGTCATCCGTGGGTCTTTAAGCCTCACCAACCTTTCGTCTTCCATGGCTGGA
                  ************************************************************

HsCD00040726      GTCTATGTCTGCAAGGCCCACAATGAGGTGGGCACTGCCCAATGTAATGTGACGCTGGAA
NM_138961.2       GTCTATGTCTGCAAGGCCCACAATGAGGTGGGCACTGCCCAATGTAATGTGACGCTGGAA
                  ************************************************************

HsCD00040726      GTGAGCACAGGGCCTGGAGCTGCAGTGGTTGCTGGAGCTGTTGTGGGTACCCTGGTTGGA
NM_138961.2       GTGAGCACAGGGCCTGGAGCTGCAGTGGTTGCTGGAGCTGTTGTGGGTACCCTGGTTGGA
                  ************************************************************

HsCD00040726      CTGGGGTTGCTGGCTGGGCTGGTCCTCTTGTACCACCGCCGGGGCAAGGCCCTGGAGGAG
NM_138961.2       CTGGGGTTGCTGGCTGGGCTGGTCCTCTTGTACCACCGCCGGGGCAAGGCCCTGGAGGAG
                  ************************************************************

HsCD00040726      CCAGCCAATGATATCAAGGAGGATGCCATTGCTCCCCGGACCCTGCCCTGGCCCAAGAGC
NM_138961.2       CCAGCCAATGATATCAAGGAGGATGCCATTGCTCCCCGGACCCTGCCCTGGCCCAAGAGC
                  ************************************************************

HsCD00040726      TCAGACACAATCTCCAAGAATGGGACCCTTTCCTCTGTCACCTCCGCACGAGCCCTCCGG
NM_138961.2       TCAGACACAATCTCCAAGAATGGGACCCTTTCCTCTGTCACCTCCGCACGAGCCCTCCGG
                  ************************************************************

HsCD00040726      CCACCCCATGGCCCTCCCAGGCCTGGTGCATTGACCCCCACGCCCAGTCTCTCCAGCCAG
NM_138961.2       CCACCCCATGGCCCTCCCAGGCCTGGTGCATTGACCCCCACGCCCAGTCTCTCCAGCCAG
                  ************************************************************

HsCD00040726      GCCCTGCCCTCACCAAGACTGCCCACGACAGATGGGGCCCACCCTCAACCAATATCCCCC
NM_138961.2       GCCCTGCCCTCACCAAGACTGCCCACGACAGATGGGGCCCACCCTCAACCAATATCCCCC
                  ************************************************************

HsCD00040726      ATCCCTGGTGGGGTTTCTTCCTCTGGCTTGAGCCGCATGGGTGCTGTGCCTGTGATGGTG
NM_138961.2       ATCCCTGGTGGGGTTTCTTCCTCTGGCTTGAGCCGCATGGGTGCTGTGCCTGTGATGGTG
                  ************************************************************

HsCD00040726      CCTGCCCAGAGTCAAGCTGGCTCTCTGGTATTG
NM_138961.2       CCTGCCCAGAGTCAAGCTGGCTCTCTGGTATGA
                  ******************************* .

Pathways

Reactome : Cell surface interactions at the vascular wall
Reactome : Hemostasis

Protein

SMART domain : IGc2 : Immunoglobulin C-2 Type
SMART domain : IG : Immunoglobulin
UniProt : Q96AP7
HPRD : 13280

Clone

Cloning Information : 179749
HIP Master Clone ID : 154850
Original Clone ID : FLH179749.01L

Organism


TAX_ID : 9606
Species Specific ID: 90952

Genome

Chromosome : 11
Map Location : 11q24.2
Ensembl : ENSG00000149564

Reagents

Labome : ESAM-antibody