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CRADD (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CRADD
Gene Name:  CASP2 and RIPK1 domain containing adaptor with death domain
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH179852.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040738

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 600nts         Open reading frame : 1 to 600
Reference Sequence Alignment

ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 680
Start on reference sequence 81
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CRADD
Symbol Nomenclature : CRADD
SYNONYM : MRT34; RAIDD
Designation : RIP-associated ICH1/CED3-homologous protein with death domain|RIP-associated protein with a death domain|caspase and RIP adaptor with death domain|death adaptor molecule RAIDD|death domain-containing protein CRADD
Full Nomenclature : CASP2 and RIPK1 domain containing adaptor with death domain
GENEID : 8738
HGNC : 2340
MIM : 603454
Vega : OTTHUMG00000170322
Target GenBank: BC017042

 

Reference Sequence Alignment

  

HsCD00040738      ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
NM_003805.3       ATGGAGGCCAGAGACAAACAAGTACTCCGCTCACTTCGCCTGGAGCTGGGTGCAGAGGTA
                  ************************************************************

HsCD00040738      TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
NM_003805.3       TTGGTGGAGGGACTGGTTCTTCAGTACCTCTACCAGGAAGGAATCTTGACGGAAAACCAT
                  ************************************************************

HsCD00040738      ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
NM_003805.3       ATTCAAGAAATCAATGCTCAAACCACAGGCCTCCGGAAAACAATGCTCCTGCTGGATATC
                  ************************************************************

HsCD00040738      CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
NM_003805.3       CTACCTTCCAGGGGCCCTAAAGCATTTGATACATTCCTAGATTCCCTACAGGAGTTTCCC
                  ************************************************************

HsCD00040738      TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
NM_003805.3       TGGGTCAGGGAGAAGCTGAAGAAGGCAAGGGAAGAGGCCATGACCGACCTGCCTGCAGGT
                  ************************************************************

HsCD00040738      GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
NM_003805.3       GACAGATTGACTGGGATCCCCTCGCACATCCTCAACAGCTCCCCATCAGACCGGCAGATT
                  ************************************************************

HsCD00040738      AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
NM_003805.3       AACCAGCTGGCCCAGAGGCTGGGCCCTGAGTGGGAGCCCATGGTGCTGTCTCTGGGACTG
                  ************************************************************

HsCD00040738      TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
NM_003805.3       TCCCAGACGGATATCTACCGCTGTAAGGCCAACCACCCCCACAACGTGCAGTCGCAGGTG
                  ************************************************************

HsCD00040738      GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
NM_003805.3       GTGGAGGCCTTCATCCGTTGGCGGCAGCGCTTCGGGAAGCAGGCCACCTTCCAGAGCCTG
                  ************************************************************

HsCD00040738      CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTTG
NM_003805.3       CACAACGGGCTGCGGGCTGTGGAGGTGGACCCCTCGCTGCTCCTGCACATGTTGGAGTGA
                  ********************************************************** .

Pathways

NCI : Caspase Cascade in Apoptosis
NCI : Ceramide signaling pathway
NCI : HIV-1 Nef: Negative effector of Fas and TNF-alpha
Panther : Apoptosis signaling pathway
WikiPathway : Apoptosis
WikiPathway : Apoptosis Modulation and Signaling

Clone

Cloning Information : 179852
HIP Master Clone ID : 154953
Original Clone ID : FLH179852.01L

Organism


TAX_ID : 9606
Species Specific ID: 8738

Genome

Chromosome : 12
Map Location : 12q21.33-q23.1
Ensembl : ENSG00000169372

Reagents

Labome : CRADD-antibody