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UBOX5 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UBOX5
Gene Name:  U-box domain containing 5
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH182323.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
HsCD00040967

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1626nts         Open reading frame : 1 to 1626
Reference Sequence Alignment

ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
TTCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1741
Start on reference sequence 116
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBOX5
Symbol Nomenclature : UBOX5
SYNONYM : RNF37; UBCE7IP5; UIP5
Designation : RING finger protein 37|U-box domain-containing protein 5|ubiquitin conjugating enzyme 7 interacting protein 5|ubiquitin-conjugating enzyme 7-interacting protein 5
Full Nomenclature : U-box domain containing 5
GENEID : 22888
Locus Tag : RP5-1187M17.9
HGNC : 17777
Vega : OTTHUMG00000031731
Target GenBank: BC000515

 

Reference Sequence Alignment

  

HsCD00040967        ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_001267584.1      ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_014948.3         ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
                    ************************************************************

HsCD00040967        GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_001267584.1      GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_014948.3         GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
                    ************************************************************

HsCD00040967        TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_001267584.1      TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_014948.3         TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
                    ************************************************************

HsCD00040967        GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_001267584.1      GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_014948.3         GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
                    ************************************************************

HsCD00040967        CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_001267584.1      CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_014948.3         CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
                    ************************************************************

HsCD00040967        ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_001267584.1      ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_014948.3         ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
                    ************************************************************

HsCD00040967        GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_001267584.1      GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_014948.3         GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
                    ************************************************************

HsCD00040967        CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_001267584.1      CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_014948.3         CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
                    ************************************************************

HsCD00040967        AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_001267584.1      AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_014948.3         AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
                    ************************************************************

HsCD00040967        ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_001267584.1      ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_014948.3         ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
                    ************************************************************

HsCD00040967        TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_001267584.1      TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_014948.3         TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
                    ************************************************************

HsCD00040967        GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_001267584.1      GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_014948.3         GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
                    ************************************************************

HsCD00040967        GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_001267584.1      GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_014948.3         GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
                    ************************************************************

HsCD00040967        GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_001267584.1      GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_014948.3         GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
                    ************************************************************

HsCD00040967        GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_001267584.1      GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_014948.3         GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
                    ************************************************************

HsCD00040967        CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_001267584.1      CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_014948.3         CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
                    ************************************************************

HsCD00040967        CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_001267584.1      CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_014948.3         CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
                    ************************************************************

HsCD00040967        CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_001267584.1      CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_014948.3         CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
                    ************************************************************

HsCD00040967        TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_001267584.1      TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_014948.3         TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
                    ************************************************************

HsCD00040967        GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_001267584.1      GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_014948.3         GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
                    ************************************************************

HsCD00040967        AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_001267584.1      AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_014948.3         AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
                    ************************************************************

HsCD00040967        CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_001267584.1      CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_014948.3         CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
                    ************************************************************

HsCD00040967        TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_001267584.1      TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_014948.3         TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
                    ************************************************************

HsCD00040967        GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
NM_001267584.1      GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCG-----GCCTGGGAGCATCCTGGGC
NM_014948.3         GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
                    ************************************     *******************

HsCD00040967        CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_001267584.1      CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_014948.3         CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
                    ************************************************************

HsCD00040967        CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
NM_001267584.1      CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGA----------------
NM_014948.3         CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
                    ********************************************                

HsCD00040967        CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
NM_001267584.1      ------------------------------------------------------------
NM_014948.3         CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
                                                                                

HsCD00040967        TTCTTG
NM_001267584.1      ------
NM_014948.3         TTCTGA
                          

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : Ubox : Modified RING finger domain
UniProt : Q86X87
UniProt : O94941
HPRD : 15605

Clone

Cloning Information : 182323
HIP Master Clone ID : 157267
Original Clone ID : FLH182323.01L

Organism


TAX_ID : 9606
Species Specific ID: 22888

Genome

Chromosome : 20
Map Location : 20p13
Ensembl : ENSG00000185019

Reagents

Labome : UBOX5-antibody